Accuracy and feasibility of three different methods for software-based image fusion in whole-body PET and CT.

BACKGROUND: Even as PET/CT provides valuable diagnostic information in a great number of clinical indications, availability of hybrid PET/CT scanners is mainly limited to clinical centers. A software-based image fusion would facilitate combined image reading of CT and PET data sets if hardware image fusion is not available. To analyze the relevance of retrospective image fusion of separately acquired PET and CT data sets, we studied the accuracy, practicability and reproducibility of three different image registration techniques. METHODS: We evaluated whole-body 18F-FDG-PET and CT data sets of 71 oncologic patients. Images were fused retrospectively using Stealth Station System, Treon (Medtronic Inc., Louisville, CO, USA) equipped with Cranial4 Software. External markers fixed to a vacuum mattress were used as reference for exact repositioning. Registration was repeated using internal anatomic landmarks and Automerge software, assessing accuracy for all three methods, measuring distances of liver representation in CT and PET with reference to a common coordinate system. RESULTS: On first measurement of image fusions with external markers, 53 were successful, 16 feasible and 2 not successful. Using anatomic landmarks, 42 were successful, 26 feasible and 3 not successful. Using Automerge Software only 13 were successful. The mean distance between center points in PET and CT was 7.69±4.96 mm on first, and 7.65±4.2 mm on second measurement. Results with external markers correlate very well and inaccuracies are significantly lower (P<0.001) than results using anatomical landmarks (10.38±6.13 mm and 10.83±6.23 mm). Analysis revealed a significantly faster alignment using external markers (P<0.001). CONCLUSIONS: External fiducials in combination with immobilization devices and breathing protocols allow for highly accurate image fusion cost-effectively and significantly less time, posing an attractive alternative for PET/CT interpretation when a hybrid scanner is not available.

[18F]choline positron emission tomography in prostate cancer patients with biochemical recurrence after radical prostatectomy: influence of antiandrogen therapy - a preliminary study.

OBJECTIVE: Our purpose was to evaluate whether antiandrogen therapy (AAT) influences [(18)F]choline PET results in patients with biochemical recurrence after radical prostatectomy (RPE). METHODS: Through a retrospective study we evaluated two groups of patients, both with histologically proven carcinoma of the prostate, who had undergone RPE and a subsequent [(18)F]choline PET because of biochemical failure (<4 ng/dl). One group consisted of 13 patients under AAT at the time of the PET examination (age range, 55-80 years; median, 68). The other group who had not undergone AAT consisted of 22 patients (age range, 48-72 years; median, 67). Our results were correlated with follow-up information related to histopathology, changes in prostate-specific antigen levels, other imaging modalities and clinical examination. Mean follow-up was 27 months. RESULTS: In patients who had undergone AAT, [(18)F]choline PET was true positive in eight out of 10 patients. The overall sensitivity in this group was 80%. In two cases [(18)F]choline PET turned out to be false negative, missing local relapse. Of the patients treated only with RPE, 10 out of 20 turned out to be true positive, resulting in a sensitivity of 50%.In all, in four patients biochemical recurrence could not be correlated to pathological findings in any of the available modalities. The difference in sensitivity between patients with and without AAT was statistically not significant (P=0.235). CONCLUSION: In patients with biochemical recurrence during AAT after RPE, [(18)F]choline PET can yield true-positive findings, even at prostate-specific antigen values of less than 4 ng/dl, and is an accurate technique for the detection of recurrence.

First experience with proteasome inhibitor treatment of radioiodine nonavid thyroid cancer using bortezomib.

PURPOSE: Radioiodine nonavid thyroid cancer (TC) is a rare disease entity with a poor prognosis. Despite a multimodal therapeutic approach including surgery, chemotherapy, and external beam radiation, radioiodine nonavid TC accounts for a high number of TC-associated deaths. The aim of this investigation was to evaluate the response rate of progressive TC patients to treatment with the proteasome inhibitor bortezomib. MATERIALS AND METHODS: Seven patients with inoperable, metastasized progressive TC proven to be radioiodine nonavid were included into this pilot study. Patients received bortezomib intravenously with a standardized dose of 1.3 mg/m on days 1, 4, 8, and 11. All patients underwent 3 therapeutic cycles with an interval of 10 days. [F]2-deoxy-2-fluoro-D-glucose positron emission tomography (F-FDG PET) and measurements of thyroglobulin levels were performed before, during, and after therapy, with a 6-week interval to post-therapeutic follow-up. RESULTS: Stable disease was seen after proteasome inhibitor therapy in 4 of the 7 patients. Two of the 7 patients showed decrease of maximum standardized uptake value in both post-therapeutic follow-up investigations, and one of these cases also had decreasing thyroglobulin levels. Two patients experienced stable disease during the posttherapeutic follow-up. Two patients showing a mixed response had an improvement in their clinical situation. One patient had rapidly progressive disease, and died 3 months after the last therapeutic cycle. Adverse events included mild polyneuropathy in 2 patients and alterations of the blood count up to WHO (World Health Organization) grade 2 in 5 patients. CONCLUSION: Proteasome inhibitor treatment with bortezomib is a promising therapeutic approach in TC patients without an established treatment alternative. The development of a specific therapeutic regimen for the treatment of radioiodine nonavid TC is warranted.

Multipolar radiofrequency ablation using 4-6 applicators simultaneously: a study in the ex vivo bovine liver.

In this study the volume and shape of coagulation zones after multipolar radiofrequency ablation (RFA) with simultaneous use of 4-6 applicators in the ex vivo bovine liver were investigated. The RF-applicators were positioned in 13 different configurations to simulate ablation of large solitary tumors and simultaneous ablation of multiple lesions with 120 kJ of applied energy/session. In total, 110 coagulation zones were induced. Standardized measurements of the volume and shape of the coagulation zones were carried out on magnetic resonance images and statistically analyzed. The coagulation zones induced with solitary applicators and with 2 applicators were imperceptibly small and incomplete, respectively. At 20mm applicator distance, the total ablated volume was significantly larger if all applicators were arranged in a single group compared to placement in 2 distant applicator groups, each consisting of 3 applicators (p=.001). The mean total coagulated volume ranged from immeasurably small (if 6 solitary applicators were applied simultaneously) to 74.7 cc (if 6 applicators at 30 mm distance between neighboring applicators were combined to a single group). Applicator distance, number and positioning array impacted time and shape. The coagulation zones surrounding groups with 4-6 applicators were regularly shaped, homogeneous and completely fused, and the axial diameters were almost constant. In conclusion, multipolar RFA with 4-6 applicators is feasible. The multipolar simultaneous mode should be applied for large and solitary lesions only, small and multiple tumors should be ablated consecutively in standard multipolar mode with up to 3 applicators.

Two-dimensional fluoroscopic navigation in posterior cruciate ligament reconstruction: a preclinical cadaver study.

OBJECTIVES: To assess the feasibility and accuracy of frameless stereotactic two-dimensional fluoroscopy-assisted guide pin (GP) placement in posterior cruciate ligament (PCL) reconstruction in human cadavers. MATERIALS AND METHODS: A total of 13 pins were placed in 7 cadaver specimens, using a fluoroscopic-based navigation technique. The knees were fixed noninvasively on a carbon baseplate. Interventions were planned on intraoperatively acquired perpendicular fluoroscopic images. A stereotactic aiming device was mounted to the carbon baseplate and adjusted according to the planned trajectories. GPs were advanced through the aiming device to the precalculated depth. GP positions were verified by image fusion of the fluoroscopic planning and control data, respectively. Measurements were scored on three occasions by one independent observer. In order to assess interobserver reliability, measurements were scored by two further independent observers on one occasion. RESULTS: The femoral cohort included seven GP placements in seven cadavers. Mean GP placement accuracy according to plan was 1.3 mm (SD 0.9 mm, range 0.3-3.8 mm) at the target point. The recorded femoral angular misalignment of GPs was 1.1 degrees (SD 0.9 degrees , range 0.2 degrees -3.3 degrees ). The tibial cohort included six GP placements in six cadavers. Mean GP placement accuracy according to the plan was 1.8 mm (SD 2.1 mm, range 0.3-9.5 mm). The recorded tibial angular misalignment of GPs was 1.4 degrees (SD 1.1 degrees , range 0.1 degrees -5 degrees ). Navigated GP implantation, as planned, was optimal in six out of seven cases in the femoral cohort and in four out of six cases in the tibial cohort. CONCLUSION: Our preliminary cadaver study suggests that the use of fluoroscopic-based navigation combined with a stereotactic targeting device may be a helpful tool to improve PCL reconstruction. In addition, this method may also be used for other minimal invasive skeletal interventions.

Accuracy of minimally invasive navigated acetabular and iliosacral fracture stabilization using a targeting and noninvasive registration device.

BACKGROUND: To assess the feasibility and accuracy of guide pin (GP) placement using a combined noninvasive patient immobilization and stereotactic targeting system in computer-assisted percutaneous pelvic fracture stabilization. METHODS: A total of 12 patients with negligible dislocated unstable pelvic fractures were enrolled in this study, performed between February 2002 and October 2005. Our original plans included 13 GP placements in the iliosacral area (SF) and 8 in the acetabular (AF) area. Patients were bedded on a noninvasive dual-vacuum immobilization device. Interventions were planned on a navigation system using intraoperatively acquired CT data. Radiodense markers glued to the skin and the immobilization device provided synchronization between virtual data set and real anatomical situation. A stereotactic targeting device was used for stabilization of GP tracking. GP positions were verified intraoperatively by CT, followed by fracture stabilization with cannulated screws. RESULTS: Mean GP placement accuracy according to plan: (1) SF-cohort: 2.8 mm (SD 2.0 mm, range 0.5-9.0 mm) at the bony entry point and 3.8 mm (SD 2.3 mm, range 0.6-9.5 mm) at the target point. (2) AF-cohort: 3.0 mm (SD 0.9 mm, range 1.6-4.9 mm) at the bony entry point and 3.9 mm (SD 1.9 mm, range 1.6-7.5 mm) at the target point. GP placement succeeded optimally in 11 out of 13 cases in the SF-cohort, and 6 out of 8 cases in the AF-cohort. The individual average dose-length product (DLP) per successful finished procedure was 1,576 mGy x cm (SD 812 mGy x cm, range 561-2,739 mGy x cm). CONCLUSION: Our findings substantiate application of the noninvasive patient immobilization and stereotactic targeting system as effective in computer-assited percutaneous stabilization of sacral bone fractures/SI joint disruptions and coronally oriented acetabular dome fractures. We recommend according to the ALARA (as low as reasonable achievable) principle: first, the kV and mAs values have to be reduced. Second, the scanned volume has to be strictly limited to the area of interest. Third, the number of control CTs have to be minimized. Also, the IsoC might be a better choice for implant tracking below 12 cm to reduce the radiation dose to the minimum. We believe that for all high-precise GP placements in the acetabular column area, further improvements in GP guidance (inhibiting pin tip slipping and detecting intraosseous GP deflection) are necessary.

Stereotactic CT-guided percutaneous stabilization of posterior pelvic ring fractures: a preclinical cadaver study.

PURPOSE: To determine the accuracy of frameless stereotactic computed tomographic (CT)-guided wire placement for percutaneous fixation of posterior pelvic ring fractures in human cadavers. MATERIALS AND METHODS: Four intact human cadavers were fixated in a double-vacuum immobilization system. A 2.5-mm helical CT dataset was obtained and transferred to the three-dimensional (3D) navigation system. In every specimen, two paths on each side (total number, 16) were defined on multiplanar reconstructions of the 3D CT datasets, simulating fixation of the iliosacral joint. An aiming device was adjusted according to the plan, and a 2.5-mm pin was advanced through the aiming device to the precalculated target point. To determine the accuracy of pin placement, a control CT scan was co-registered to the planning CT scan (with the planned trajectories). The distance between the planned and achieved positions of the pins (3D accuracy) was calculated in millimeters. RESULTS: The mean 3D accuracy was 1.84 mm +/- 0.9 (standard deviation) at the bone entrance point and 2.5 mm +/- 1.2 at the target, as determined with image fusion between the planning CT scan and the control CT scan with the pins in place. CONCLUSIONS: The described technique enables accurate placement of pins in the pelvis and may be useful for percutaneous orthopedic procedures.

Frameless stereotactic cannulation of the foramen ovale for ablative treatment of trigeminal neuralgia.

OBJECTIVE: Ablative neurosurgical treatment of trigeminal neuralgia, including percutaneous radiofrequency thermocoagulation, requires cannulation of the foramen ovale. To maximize patient security and cannulation success, a frameless stereotactic system was evaluated in a phantom study, a cadaveric study, and a preliminary clinical trial. METHODS: Frameless stereotaxy using an optical navigation system, an aiming device, and a noninvasive vacuum mouthpiece-based registration and patient fixation technique was used for the targeting of a test body based on 1-, 3-, and 5-mm axial computed tomographic slices and of the foramen ovale in three cadavers and 15 patients based on 3-mm axial computed tomographic slices. RESULTS: The mean normal (x/y) localization accuracy/standard deviation (n = 360) was 1.31/0.67 mm (1-mm slices), 1.38/0.65 mm (3-mm slices), and 1.84/0.96 mm (5-mm slices). Significantly better results were achieved with 1- and 3-mm slices when compared with 5-mm slices (P < 0.001). The foramen ovale (3 x 6 mm) was successfully cannulated at the first attempt in all cadavers and patients, which indicates clinical localization accuracies better than 1.5 mm in the anteroposterior and 3 mm in the medial-lateral directions. CONCLUSION: Based on the noninvasive Vogele-Bale-Hohner vacuum mouthpiece, there is no need for invasive head clamp fixation. Imaging, real laboratory simulation, and the actual surgical intervention can be separated in time and location. The presented data suggest that frameless stereotaxy is a predictable and reproducible procedure, which may enhance patient security and cannulation success independent of the surgeon's experience.

Reproducibility of patient positioning for fractionated extracranial stereotactic radiotherapy using a double-vacuum technique.

BACKGROUND AND PURPOSE: Precise reproducible patient positioning is a prerequisite for conformal fractionated radiotherapy. A fixation system based on double-vacuum technology is presented which can be used for conventional as well as hypofractionated stereotactic extracranial radiotherapy. MATERIAL AND METHODS: To form the actual vacuum mattress, the patient is pressed into the mattress with a vacuum foil which can also be used for daily repositioning and fixation. A stereotactic frame can be positioned over the region of interest on an indexed base plate. Repositioning accuracy was determined by comparing daily, pretreatment, orthogonal portal images to the respective digitally reconstructed radiographs (DRRs) in ten patients with abdominal and pelvic lesions receiving extracranial fractionated (stereotactic) radiotherapy. The three-dimensional (3-D) vectors and 95% confidence intervals (CI) were calculated from the respective deviations in the three axes. Time required for initial mold production and daily repositioning was also determined. RESULTS: The mean 3-D repositioning error (187 fractions) was 2.5 +/- 1.1 mm. The largest single deviation (10 mm) was observed in a patient treated in prone position. Mold production took an average of 15 min (10-30 min). Repositioning times are not necessarily longer than using no positioning aid at all. CONCLUSION: The presented fixation system allows reliable, flexible and efficient patient positioning for extracranial stereotactic radiotherapy.

Multimodality cranial image fusion using external markers applied via a vacuum mouthpiece and a case report.

PURPOSE: To present a simple and precise method of combining functional information of cranial SPECT and PET images with CT and MRI, in any combination. MATERIAL AND METHODS: Imaging is performed with a hockey mask-like reference frame with image modality-specific markers in precisely defined positions. This frame is reproducibly connected to the VBH vacuum mouthpiece, granting objectively identical repositioning of the frame with respect to the cranium. Using these markers, the desired 3-D imaging modalities can then be manually or automatically registered. This information can be used for diagnosis, treatment planning, and evaluation of follow-up, while the same vacuum mouthpiece allows precisely reproducible stereotactic head fixation during radiotherapy. RESULTS: 244 CT and MR data sets of 49 patients were registered to a root square mean error (RSME) of 0.9 mm (mean). 64 SPECT-CT fusions on 18 of these patients gave an RMSE of 1.4 mm, and 40 PET-CT data sets of eight patients were registered to 1.3 mm. An example of the method is given by means of a case report of a 52-year-old patient with bilateral optic nerve meningioma. CONCLUSION: This technique is a simple, objective and accurate registration tool to combine diagnosis, treatment planning, treatment, and follow-up, all via an individualized vacuum mouthpiece. Especially for low-resolution PET and even more so for some very diffuse SPECT data sets, activity can now be accurately correlated to anatomic structures.