ABSTRACT
OBJECTIVE: To evaluate the effectiveness of a polyclonal immunoglobulin (Ig) preparation containing IgG, IgM, and IgA as an adjunctive therapy for septic shock. DESIGN: Prospective, randomized clinical trial. SETTING: A clinical immunology ward at the center for internal medicine in a university hospital. PATIENTS: Fifty-five patients with septic shock were randomly allocated to two groups according to criteria of septic shock. INTERVENTION: One group of patients (n = 27) received a commercially available immunoglobulin preparation (containing high titers of antibodies specific for determinants to bacterial endotoxin) during the first 3 days after inclusion in the study. The other randomized group (n = 28) did not receive any immunoglobulin preparation. MEASUREMENTS AND MAIN RESULTS: During the period of less than or equal to 6 wks after the beginning of clinically apparent septic shock, death related to the septic process occurred in one (4%) of 27 patients who received immunoglobulin. By comparison, nine (32%) of 28 control group patients died during this period (p less than .01). Within the first 48 hrs after onset of the clinically apparent septic process, significantly increased activity of circulating endotoxin and simultaneously decreased specific IgG serum titers to lipid A were detected in the group of nonsurvivors. CONCLUSION: Administration of a polyclonal immunoglobulin preparation in the early phase of septic shock was associated with significantly improved survival.
Subject(s)
Bacterial Infections/therapy , Gram-Negative Bacteria , Immunization, Passive , Shock, Septic/therapy , Antibodies, Bacterial/blood , Bacterial Infections/immunology , Bacterial Infections/mortality , Endotoxins/blood , Female , Gram-Negative Bacteria/immunology , Humans , Immunization, Passive/methods , Immunoglobulin G/analysis , Immunoglobulin M/analysis , Lipopolysaccharides/immunology , Male , Prospective Studies , Shock, Septic/immunology , Shock, Septic/mortalitySubject(s)
DNA Polymerase I/metabolism , DNA-Directed DNA Polymerase/metabolism , Melanoma/enzymology , RNA-Directed DNA Polymerase/metabolism , DNA Polymerase II/metabolism , DNA Polymerase III/metabolism , DNA-Directed DNA Polymerase/immunology , Epitopes , Humans , RNA-Directed DNA Polymerase/immunologyABSTRACT
An RNA-direct DNA polymerase was purified from human melanoma tissue by successive column chromatography on DEAE-cellulose (DE-23 and DE-52) and phosphocellulose. The purified reverse transcriptase has a mol. wt. of 68,000, a pH optimum of 8.0, a Mn2+ optimum of 0.6 mM, and a KCl optimum of 60 mM. The purified enzyme transcribes (rA)n - (dT)12, (rC)n - (dG)18, (Ome-rC)n - (dG)18 and a 70s RNA from Rauscher leukemia virus (RLV), but failed to transcribe (dA)n - (dT)12. This enzyme has no terminal deoxynucleotidyl transferase activity. Serological studies have shown that the reverse transcriptase from human melanoma tissue is antigenically not related to DNA polymerases from Simian sarcoma virus (SiSV), Avian myeloblastosis virus (AMV), RLV, and human spleen of a patient with myelofibrosis. The purified enzyme showed a close antigenic resemblance to DNA polymerases from baboon endogenous virus (BEV) and rhabdomyosarcoma virus (RD-114), the endogenous virus of the cat.
Subject(s)
Melanoma/enzymology , RNA-Directed DNA Polymerase/metabolism , Animals , Cats , Cross Reactions , Epitopes , Haplorhini , Humans , Melanoma/microbiology , RNA-Directed DNA Polymerase/immunology , RNA-Directed DNA Polymerase/isolation & purification , Retroviridae/enzymologyABSTRACT
Two protein bands of water soluble extracts of psoriatic scales, which appear intensified in disc-electrophoretical separation, were isolated and their amino acid compositions were determined. It could be demonstrated that band 9 was rich of glycine, alanine, aspartic acid, leucine, and serine and that band 10 was rich of glycine, threonine, and serine. Both proteins contained an amino-sugar (galactosamine).
Subject(s)
Amino Acids/analysis , Proteins/analysis , Psoriasis/metabolism , Alanine/analysis , Electrophoresis, Disc , Galactosamine/analysis , Glycine/analysis , Humans , Leucine/analysis , Proteins/isolation & purification , Serine/analysis , Skin/analysis , Threonine/analysisABSTRACT
Disc-electrophoretic investigations of psoriatic scale homogenates (15000 x g supernatant) revealed several different phosphatase activities. At pH 5 either five different phosphatase active bands (substrates: p-nitrophenylphosphate, naphthylphosphate) or three different bands (substrate: glycerophosphate) could be obtained. At pH 7 only one band (substrate: p-nitrophenylphosphate) showed phosphatase activity. At pH 9.9 either two bands (substrate: p-nitrophenylphosphate) or three bands (substrate: glycerophosphate) could be demonstrated. the acid p-nitrophenylphosphatase activity of the psoriatic specific bands "9" and "10" could be distinguished by their different fluoride and tartrate susceptibility. Also the alkaline glycerophosphatase activities could be differentiated by distinct Ca and Mg susceptibility.
