The role of offloading intentions on future-oriented thinking.

Offloading (e.g., using Google calendar reminders) has been shown to improve prospective memory (PM). One unstudied aspect of PM offloading is whether having reminders reduces our future-oriented thinking about PM intentions in contexts in which the intention cannot be fulfilled. In the current study, participants were given two blocks of an ongoing lexical decision task. Prior to beginning the task, participants formed an intention to make a special response to PM targets only in block 2. Participants in the reminder condition had the PM intention displayed at the top of the screen in block 2, whereas those in the no-reminder condition did not. To assess activation of the intention out of context, PM lures (Experiment 1) or thought probes (Experiments 2 and 3) were presented in block 1. Results showed that reminders improved PM performance in block 2 but did not reduce lure interference or PM-related thoughts in block 1. These findings suggest that offloaded memory representations remain as activated and accessible as non-offloaded representations outside the context in which intentions can be fulfilled.

Task sequencing does not systematically affect the factor structure of cognitive abilities.

Latent variable analyses of cognitive abilities are among the major means by which cognitive psychologists test theories regarding the structure of human cognition. Models are fit to observed variance-covariance structures, and the fit of those models are compared to assess the merits of competing theories. However, an often unconsidered and potentially important methodological issue is the precise sequence in which tasks are delivered to participants. Here we empirically tested whether differences in task sequences systematically affect the observed factor structure. A large sample (N = 587) completed a battery of 12 cognitive tasks measuring four constructs: working memory, long-term memory, attention control, and fluid intelligence. Participants were assigned to complete the assessment in one of three sequences: fixed and grouped by construct vs. fixed and interleaved across constructs vs. random by participant. We generated and tested two hypotheses: grouping task sequences by construct (i.e., administering clusters of tasks measuring a cognitive construct consecutively) would (1) systematically increase factor loadings and (2) systematically decrease interfactor correlations. Neither hypothesis was supported. The measurement models were largely invariant across the three conditions, suggesting that latent variable analyses are robust to such subtle methodological differences as task sequencing.

Strategic monitoring improves prospective memory: A meta-analysis.

Monitoring the environment for target events that trigger prospective memory (PM) retrieval requires cognitive resources, reflected by costs to ongoing task performance (i.e., worse accuracy and/or slower response times). Strategic monitoring refers to the use of context to engage or disengage monitoring when a PM target is anticipated or unanticipated. Laboratory strategic monitoring studies have found mixed results as to whether context specification improves PM performance. This study employed a meta-analytic technique to assess the overall effect of context specification on PM performance and ongoing task metrics of strategic monitoring. Overall, context specification improved PM performance when the target was anticipated and improved ongoing task performance (speed and accuracy) when the target was not anticipated. Moderator analyses revealed the degree of slowing in anticipated contexts predicted how much context specification improved PM performance. However, the benefits to PM performance from context specification differed by the type of procedure used. PM performance was improved when context changes could be predicted during blocked or proximity procedures, but not when context varied randomly in trial-level procedures. These results provide insights into the mechanisms underlying strategic monitoring and guidance for researchers on which procedures to be use depending on the theory-driven questions.

Dissociating proactive and reactive control in older adults.

The Dual Mechanisms of Control framework predicts that age-related declines should be most prominent for tasks that require proactive control, while tasks requiring reactive control should show minimal age differences in performance. However, results from traditional paradigms are inconclusive as to whether these two processes are independent, making it difficult to understand how these processes change with age. The present study manipulated the proportion congruency in a list-wide (Experiments 1 and 2) or item-specific (Experiment 1) fashion to independently assess proactive and reactive control, respectively. In the list-wide task, older adults were unable to proactively bias attention away from word processing based on list-level expectancies. Proactive control deficits replicated across multiple task paradigms, with different Stroop stimuli (picture-word, integrated color-word, separated color-word), and different behavioral indices (Stroop interference, secondary prospective memory). In contrast, older adults were successfully able to reactively filter the word dimension based on item-specific expectancies. These findings provide unambiguous support that aging is associated with declines in proactive, but not reactive, control. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

Adult age differences in subjective context retrieval in dual-list free recall.

Age-related episodic memory deficits imply that older and younger adults differentially retrieve and monitor contextual features that indicate the source of studied information. Such differences have been shown in subjective reports during recognition and cued recall as well as process estimates derived from computational models of free recall organisation. The present study extends the subject report method to free recall to characterise age differences in context retrieval and monitoring, and to test assumptions from a context-based computational model. Older and younger adults studied two lists of semantically related words and then recalled from only the first or second list. After each recall, participants indicated their subjective context retrieval using remember/know judgments. Compared to younger adults, older adults showed lower recall accuracy and subjective reports of context retrieval (i.e., remember judgments) that were less specific to correct recalls. These differences appeared after first-recall attempts. Recall functions conditioned on serial positions were more continual across correct recalls from target lists and intrusions from non-target lists for older than younger adults. Together with other analyses of context retrieval and monitoring reported here, these findings suggest that older adults retrieved context less distinctively across the recall period, leading to greater perceived similarity for temporally contiguous lists.

Benefits from prospective memory offloading depend on memory load and reminder type.

Prospective memory (PM) refers to the ability to remember to complete a task at the appropriate moment in the future. Past research has found reminders can improve PM performance in both laboratory and naturalistic settings, but few projects have examined the circumstances when and what types of reminders are most beneficial. Three experiments in the present study tested the influence of reminders in an event-based PM task under different cognitive loads. An additional study examined how effective reminders of different types were. In Experiments 1 (specific targets) and 2 (nonspecific targets), load was manipulated by having participants respond to a single (low load) or multiple (high load) targets. In Experiment 3, the association between target-action word pairs was manipulated by presenting strongly associated pairs (low load) or weakly associated pairs (high load). Experiment 4 used target-action word pairs and varied the type of reminder. Participants in the reminder conditions had target (Experiments 1 and 2), target and action (Experiment 3) or target and/or action (Experiment 4) reminders listed at the top of the screen throughout the PM task. Across the first 3 experiments, it was found that the benefit of reminders was greater under high load than low load conditions. Experiment 4 found that target-action reminders improved PM, while target-only or action-only reminders did not. Importantly, the improvements in PM from reminders occurred without cost to ongoing task performance. Together these results suggest that reminders can be beneficial for reducing PM failures, particularly under high load, without the potential downside of increased effort expenditure. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

A multimodal analysis of sustained attention in younger and older adults.

Age-related cognitive decline has been attributed to processing speed differences, as well as differences in executive control and response inhibition. However, recent research has shown that healthy older adults have intact, if not superior, sustained attention abilities compared to younger adults. The present study used a combination of reaction time (RT), thought probes, and pupillometry to measure sustained attention in samples of younger and older adults. The RT data revealed that, while slightly slower overall, older adults sustained their attention to the task better than younger adults, and did not show a vigilance decrement. Older adults also reported fewer instances of task-unrelated thoughts and reported feeling more motivated and alert than younger adults, despite finding the task more demanding. Additionally, older adults showed larger, albeit later-peaking, task-evoked pupillary responses (TEPRs), corroborating the behavioral and self-report data. Finally, older adults did not show a shallowing of TEPRs across time, corroborating the finding that their RTs also did not change across time. The present findings are interpreted in light of processing speed theory, resource-depletion theories of vigilance, and recent neurological theories of cognitive aging. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

Individual differences in memory and attention processes in prospective remembering.

Successful prospective memory (PM) involves not only detecting that an environmental cue requires action (i.e., prospective component), but also retrieval of what is supposed to be done at the appropriate moment (i.e., retrospective component). The current study examined the role of attention and memory during PM tasks that placed distinct demands on detection and retrieval processes. Using a large-scale individual differences design, participants completed three PM tasks that placed high demands on detection (but low demands on retrieval) and three tasks that placed high demands on retrieval (but low demands on detection). Additionally, participants completed three attention control, retrospective memory, and working memory tasks. Latent variable structural equation modeling showed that the prospective and retrospective components of PM were jointly influenced by multiple cognitive abilities. Critically, attention and retrospective memory fully mediated the relation between working memory and prospective memory. Furthermore, only attention uniquely predicted PM detection, whereas only retrospective memory uniquely predicted PM retrieval. These findings highlight the value of independently assessing different PM components and suggest that both attention and memory abilities must be considered to fully understand the dynamic processes underlying prospective remembering.

Performance on the processing portion of complex working memory span tasks is related to working memory capacity estimates.

Individual differences in working memory capacity (WMC) have long been known to relate to performance in domains outside of WM, including attentional control, long-term memory, problem-solving, and fluid intelligence to name a few. Complex span WM tasks, composed of a processing component and a storage component, are often used to index WMC in these types of investigations. Capacity estimates are derived from performance on the storage component only, while processing performance is often largely ignored. Here, we explore the relationship between processing performance and WMC in a large dataset for each of three complex span tasks to better characterize how the components of these tasks might be related. We provide evidence that enforcing an 85% or better accuracy criterion for the processing portion of the task results in the removal of a disproportionate number of individuals exhibiting lower WMC estimates. We also find broad support for differences in processing task performance, characterized according to both accuracy and reaction time metrics, as a function of WMC. We suggest that researchers may want to include processing task performance measures, in addition to capacity estimates, in studies using complex span tasks to index WMC. This approach may better characterize the relationships between complex span task performance and performance in disparate domains of cognition.

Individual Differences in Disqualifying Monitoring Underlie False Recognition of Associative and Conjunction Lures.

The current study leveraged experimental and individual differences methodology to examine whether false memories across different list-learning tasks arise from a common cause. Participants completed multiple false memory (associative and conjunction lure), working memory (operation and reading span), and source monitoring (verbal and picture) tasks. Memory discriminability in the associative and conjunction tasks loaded onto a single (general) factor and were unaffected by warnings provided at encoding. Consistent with previous research, source-monitoring ability fully mediated the relation between working memory and false memories. Moreover, individuals with higher source monitoring-ability were better able to recall contextual information from encoding to correctly reject lures. These results suggest that there are stable individual differences in false remembering across tasks. The commonality across tasks may be due, at least in part, to the ability to effectively use disqualifying monitoring processes.

Proteome and secretome profiling of zinc availability in Cryptococcus neoformans identifies Wos2 as a subtle influencer of fungal virulence determinants.

BACKGROUND: Fungal infections impact over 25% of the global population. For the opportunistic fungal pathogen, Cryptococcus neoformans, infection leads to cryptococcosis. In the presence of the host, disease is enabled by elaboration of sophisticated virulence determinants, including polysaccharide capsule, melanin, thermotolerance, and extracellular enzymes. Conversely, the host protects itself from fungal invasion by regulating and sequestering transition metals (e.g., iron, zinc, copper) important for microbial growth and survival. RESULTS: Here, we explore the intricate relationship between zinc availability and fungal virulence via mass spectrometry-based quantitative proteomics. We observe a core proteome along with a distinct zinc-regulated protein-level signature demonstrating a shift away from transport and ion binding under zinc-replete conditions towards transcription and metal acquisition under zinc-limited conditions. In addition, we revealed a novel connection among zinc availability, thermotolerance, as well as capsule and melanin production through the detection of a Wos2 ortholog in the secretome under replete conditions. CONCLUSIONS: Overall, we provide new biological insight into cellular remodeling at the protein level of C. neoformans under regulated zinc conditions and uncover a novel connection between zinc homeostasis and fungal virulence determinants.

Clinical, pathologic, and epidemiologic features of nocardioform placentitis in the mare.

Placentitis is the leading cause of infectious abortion in the horse and contributes to roughly 19% of all abortions in the United States. A type of placental infection, nocardioform placentitis (NP) is associated with gram-positive branching actinomycetes localized within the ventral body of the feto-maternal interface to create a lymphoplasmacytic mucoid lesion. While the etiology of this disease is poorly described, this placental infection continues to cause episodic abortions in addition to weak and/or growth retarded neonates. The goal of the present study was to perform a comprehensive analysis of pregnancies associated with a nocardioform-affected placenta and make inferences into the epidemiology of this elusive disease. To do so, 264 mares were enrolled in the study, with 145 as having suspected disease (n = 145; NP) either based on pregnancy-related complications or postpartum placental evaluation, while an additional 119 were enrolled as healthy pregnancies (n = 119; CON). Following diagnosis as either NP or CON based on gross and histopathology at the University of Kentucky Veterinary Diagnostic Laboratory, information was gathered on the mares and neonates for comparisons between diseased and healthy pregnancies. Clinically, a significant portion of diseased mares had clinical indications of NP, including premature mammary gland development, thickening of the placenta noted on transrectal ultrasonography, and separation between the chorioallantois and endometrium noted on abdominal ultrasonography, while vulvar discharge was not commonly noted. Additionally, NP was correlated with increased mare age, decreased gestational length, and decreased neonatal weight, although neonatal IgG and WBC were comparable to CON. Incidence of NP was not correlated with last breeding date, pre- and post-breeding therapeutics, parity, prophylactic medications, or housing. Additionally, NP did not affect postpartum fertility. While NP was associated with a poor neonatal outcome (abortion and/or growth retarded neonate), this did not appear to be influenced by the bacteria isolated (Amycolatopsis spp. vs. Crossiella equi), and mares diagnosed with NP do not appear to be infectious to other pregnant mares nor have repetitive years of the disease. Interestingly, lesion size was positively correlated with last breeding date, as mares bred later in the breeding season correlating with a larger placental lesion. In conclusion, while the etiology of NP continues to elude researchers, the epidemiology of this disease has gained clarity, providing inferences into the management of suspect mares.

The influence of semantic context on false memories.

Although false memories have largely been examined with the Deese-Roediger-McDermott (DRM) paradigm, little research has focused on the semantic context in which associates are encoded. Across three experiments, we varied semantic context during a sentence-processing task with DRM associates embedded within sentences. More meaningful sentences resulted in greater memory errors (Experiment 1). Furthermore, providing contextual information to discriminate old from new items did not reduce false alarms relative to encoding words in isolation when sentences converged on the meaning of the critical lure (Experiment 2), and actually increased memory errors (Experiment 3). These results suggest that semantic context that allows for meaningful relational processing of items within-lists and that converge on the semantic meaning of the critical lure increases the likelihood that the list theme is identified, resulting in more errors at test.

Serum amyloid A, Serum Amyloid A1 and Haptoglobin in pregnant mares and their fetuses after experimental induction of placentitis.

Serum amyloid A (SAA) and Haptoglobin (Hp) are acute phase proteins, produced during inflammation, such as placentitis. In horses, SAA and SAA1 are protein coding genes. Objectives were to analyze SAA and Hp concentrations and relative abundance of SAA, SAA1 and Hp mRNA transcript in maternal and fetal tissues after experimental induction of placentitis or mares of a control group. Serum Amyloid A family proteins were in marked abundance in the stroma of the endometrium and chorioallantois associated with inflammatory cells. Maternal plasma SAA concentrations were greater (P = 0.01) in mares with experimentally induced placentitis compared to those of the control group. Maternal Hp from the groups were not different, but fetal Hp concentrations of mares with experimentally induced placentitis were greater (P = 0.02). Maternal plasma SAA and Hp concentrations were greater than fetal plasma concentrations in mares with experimentally induced placentitis (P < 0.05). Relative abundance of SAA mRNA transcript was greater in the maternal, fetal liver and chorioallantois of mares with experimentally induced placentitis (P < 0.05) compared to those in the control group. Interestingly, relative abundance of SAA1 mRNA transcript was greater in the chorioallantois of mares with experimentally induced placentitis (P < 0.05). The SAA and Hp concentrations, therefore, were greater in mares with induced placentitis. Furthermore, relative abundance of SAA1 mRNA transcript is specifically greater in the chorioallantois of mares with placentitis, which warrants further studies to elucidate the immunological response of SAA1 in the chorioallantois of mares with placentitis.

Alterations of Circulating Biomarkers During Late Term Pregnancy Complications in the Horse Part I: Cytokines.

Equine abortions are attributed to both infectious and noninfectious causes. Clinical extrapolations are often made from the experimental model for ascending placentitis towards other causes of fetal compromise, including various markers of inflammation, including the cytokines IL-2, 5, IL-6, IL-10, IFNγ, and TNF. It is unknown if these cytokine changes are noted under field conditions, or if they increase preceding other pregnancy related complications. To assess this, Thoroughbred mares (n = 702) had weekly blood obtained beginning in December 2013 and continuing until parturition. Fetal membranes were submitted to the UKVDL for complete gross and pathologic assessment and classified as either ascending placentitis (n = 6), focal mucoid placentitis (n = 6), idiopathic abortion (n = 6) or control (n = 20). Weekly serum samples were analyzed via immunoassay for concentrations of IL-2, IL-5, IL-6, IL-10, IFNγ, and TNF. For both focal mucoid placentitis and ascending placentitis, an increase (P < .05) in the concentrations of IL-2, IL-5, IL-6, IL-10, IFNγ, and TNF was noted preceding parturition in comparison to controls. Cytokine profiles preceding idiopathic abortion did not differ from controls. In conclusion, serum cytokines may be considered potential biomarkers for the prediction of placental infection, while no changes in cytokine profiles were noted when noninfectious causes of abortion occurred. Additionally, this is the first study to report an increase in cytokines during the disease process of focal mucoid placentitis, the etiology of which includes Nocardioform placentitis.

The imbalance of the Th17/Treg axis following equine ascending placental infection.

Ascending placentitis is a leading cause of abortion in the horse, but adaptive immune response to this disease is unknown. To evaluate this, sub-acute placentitis was experimentally-induced via trans-cervical inoculation of S. zooepidemicus, and endometrium and chorioallantois was collected 8 days later (n = 6 inoculated/n = 6 control). The expression of transcripts relating to Th1, Th2, Th17, and Treg maturation was assessed via RNASeq. IHC of transcription factors relating to each subtype in the same tissues (Th1: TBX21, Th2: GATA3, Th17: IRF4, Treg: FOXp3). An immunoassay was utilized to assess circulating cytokines (Th1: IFNg, IL-2; Th2: IL-4, IL-5; Th17: IL-17, IL-6; Treg: IL-10, GM-CSF). An increase in Th1 and Th17-related transcripts were noted in the chorioallantois, although no alterations were seen in the endometrium. Th2 and Treg-related transcripts altered in a dysregulated manner, as some transcripts increased in expression while others decreased. Immunolocalization of Th1, Th2, and Th17 cells was increased in diseased chorioallantois, while no Treg cells were noted in the diseased tissue. Secreted cytokines relating to Th1 (IFNg, IL-2), Th17 (IL-6), Th2 (IL-5), and Treg (IL-10) populations increased in maternal circulation eight days after inoculation. In conclusion, the Th1/Th17 response to ascending placentitis occurs primarily in the chorioallantois, indicating the adaptive immune response to occur in fetal derived placental tissue. Additionally, ascending placentitis leads to an increase in the helper T cell populations (Th1/Th17/Th2) while decreasing the Treg response. This increase in Th17-related responses alongside a diminishing Treg-related response may precede or contribute to fetal demise, abortion, or preterm labor.

Changes in circulating concentrations of testosterone and estrone sulfate after human chorionic gonadotropin administration and subsequent to castration of 2-year-old stallions.

Reproductive steroids testosterone (T) and estrone sulfate (E1S) are used as diagnostic markers for cryptorchidism in horses. The human chorionic gonadotropin (hCG) stimulation test is used as a diagnostic aid because administration of this hormone results in greater incremental differences in circulating steroid concentrations. Thoughts regarding optimal sampling times following hCG administration, however, are inconsistent. Additionally, determination of half-life of these steroids is important in postsurgical samples to confirm complete removal of testicular tissue. Objectives of this study, therefore, were to determine optimal sampling periods for peak T and E1S after hCG administration and half-life of these steroids after castration. Eight pony stallions were randomly assigned to control or treatment groups (5000 IU hCG). Blood samples were collected following hCG administration. Subsequently, stallions were castrated and blood samples were collected post-castration. The T concentrations were greatest at 72 h after hCG and were greater (P < 0.02) in samples from hCG-treated than control animals: 9,903.4 ± 384 and 784.0 ± 192 pg/mL, respectively (Mean ± SEM). The T concentrations were also greater at 1, 12, 24, 48 and 96 h. The E1S concentrations did not change after administration of hCG. The T response to hCG administration was biphasic with a maximal response between 48-96 h after administration. Half-lives of T and E1S were 1.1 and 0.7 h, respectively, and concentration of T and E1S was similar to that of geldings at 24 h post-castration, which, therefore, should be considered an optimal time to ensure complete castration has occurred.

Radiotherapy-specific interprofessional learning through simulation.

INTRODUCTION: Interprofessional learning (IPL) is a vital aspect of training in radiation oncology professions, yet is rarely delivered to those professionals who work most closely together in clinical practice. Scenario-based learning using simulation facilities provides a unique opportunity to facilitate this learning and this project aimed to determine the impact and value of this initiative. METHODS: Small groups comprising post-graduate diploma pre-registration therapeutic radiographers, medical physics trainees and radiation oncology registrars were challenged with 4 plausible and challenging radiotherapy scenarios within an academic simulation centre. Pre- and post-event completion of the "Readiness for Interprofessional Learning Scale" measured impact and a Likert-style survey gathered feedback from participants. RESULTS: The session increased participants' teamwork and collaboration skills as well as strengthening professional identities. Participants reported high levels of enjoyment related to collaborative working, communication and observing other professionals deploying their technical skills and specialist knowledge. CONCLUSION: Although beneficial, simulated scenarios offering equal opportunities for engagement across the professions are challenging to plan and timetabling issues between the 3 groups present significant difficulties. The safe environment and unique opportunity for these groups to learn together was particularly well received and future oncology-specific simulated scenario sessions are planned with larger cohorts. IMPLICATIONS FOR PRACTICE: Simulated scenario training can be used to improve team working across the radiotherapy interprofessional team and may have wider use in other specialist interdisciplinary team development.

Wait a second . . . Boundary conditions on delayed responding theories of prospective memory.

Research suggests that forcing participants to withhold responding for as brief as 600 ms eliminates one of the most reliable findings in prospective memory (PM): the cue focality effect. This result undermines the conventional view that controlled attentional monitoring processes support PM, and instead suggests that cue detection results from increased response thresholds that allow more time for PM information to accumulate. Given the significance of such findings, it is critical to examine the generalizability of the delay mechanism. Experiments 1-4 examined boundary conditions of the delay theory of PM, whereas Experiment 5 more directly tested contrasting theoretical predictions from monitoring theory (e.g., multiprocess framework) and delay theory. Using the same (Experiment 1) or conceptually similar (Experiment 2) delay procedure and identical cues (nonfocal "tor" intention) from the original study failed to show any influence of delay on performance. Using a different nonfocal intention (first letter "S") similarly did not influence performance (Experiment 3), and the difference between focal and nonfocal cue detection was never completely eliminated even with delays as long as 2,500 ms (Experiment 4). Experiment 5 did find the anticipated reduction in the focality effect with increased delays with a larger sample (n = 249). However, the focality effect was not moderated by attention control ability despite the fact that participants with impoverished attention control should benefit most from the delay procedure. These results suggest that any theory of PM that considers only a delay mechanism may not fully capture the dynamic attention processes that support cue detection. (PsycInfo Database Record (c) 2021 APA, all rights reserved).