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Virol J ; 9: 79, 2012 Apr 11.
Article in English | MEDLINE | ID: mdl-22495040

ABSTRACT

Viral diseases constitute obstacles to pepper production in the world. In Nigeria, pepper plants are primarily affected by pepper veinal mottle virus (PVMV), Cucumber mosaic virus (CMV), Pepper leaf curl Virus (TLCV), Tobacco mosaic virus (TMV), Pepper mottle virus (PMV) and a host of other viruses. The experiment was carried out with a diagnostic survey on the experimental field of the National Horticultural Research Institute, Ibadan, Nigeria and on pepper farms in six local government areas within Ibadan Oyo State, Nigeria, forty samples were collected from each of the farms. Diseased samples were obtained from the field and taken to the laboratory for indexing. In ELISA test some of the samples from the pepper farms showed positive reaction to single infection with PVMV (36.79%), CMV (22.14%) while some others showed positive reaction to mixed infection of the two viruses (10%) but some also negative reaction to PVMV and CMV antisera (31.07).


Subject(s)
Capsicum/virology , Cucumovirus/isolation & purification , Plant Diseases/virology , Potyvirus/isolation & purification , Enzyme-Linked Immunosorbent Assay/methods , Nigeria , Virology/methods
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