ABSTRACT
BACKGROUND AND AIMS: Inflammatory bowel disease [IBD] can impair patients' functional capacity with significant negative effects on their quality of life. Our aim was to determine the impact of IBD diagnosis on fitness levels and to assess the levels of engagement in physical activity and fatigue in IBD patient before and after diagnosis. METHODS: A prospective multi-centre cross-sectional study was performed. Patients diagnosed with IBD in the previous 18 months were recruited. Inclusion criteria included clinical remission and/or no treatment changes within the previous 6 months. Physical exercise levels were assessed by the Godin score and fatigue levels was assessed by the functional assessment of chronic illness therapy [FACIT] score. RESULTS: In total, 158 patients (100 Crohn's disease [CD]) were recruited. Mean age was 35.1 years (95% confidence interval [CI] ± 2.0). Gender distribution was approximately equal [51.3% male]. The Mean Harvey Bradshaw and Simple Clinical Colitis Activity indices were 2.25 [95% CI ± 0.40] and 1.64 [95% CI ± 0.49], respectively. The mean Godin score difference before and after IBD diagnosis was 6.94 [p = 0.002]. Patients with ulcerative colitis [UC] [41.8%] were more likely than patients with CD [23.0%] to reduce their exercise levels [p = 0.04]. FACIT scores were lower in patients who had experienced relapses [p = 0.012] and had severe disease [p = 0.011]. Approximately one-third of patients reduced their activity level following IBD diagnosis. CONCLUSIONS: Patients were significantly less physically active after a diagnosis of IBD and this was more apparent in UC. Identification of the risk factors associated with loss of fitness levels would help to address the reduced patient quality of life.
Subject(s)
Exercise , Inflammatory Bowel Diseases/psychology , Adolescent , Adult , Colitis, Ulcerative/diagnosis , Colitis, Ulcerative/psychology , Crohn Disease/diagnosis , Crohn Disease/psychology , Cross-Sectional Studies , Exercise/psychology , Female , Humans , Inflammatory Bowel Diseases/diagnosis , Male , Prospective Studies , Severity of Illness Index , Young AdultABSTRACT
Durante los últimos años, se ha acumulado evidencia quedemuestra que la regulación del ciclo celular de las células es esencialpara establecer tolerancia y suprimir autoinmunidad. Aunquela apoptosis se ha considerado un mecanismo importante enel control del desarrollo de tolerancia y autoinmunidad, la regulacióndel ciclo celular también constituye una vía alternativaen la prevención de la autoreactividad. Diferentes moléculas asociadasal ciclo celular actúan como supresoras de la autoinmunidad.Se ha demostrado recientemente que los inhibidores delciclo celular p21 y p27 controlan la tolerancia de las células T,mientras que p21 también limita el desarrollo de la autoinmunidad.En esta revisión exploraremos los efectos de p21 y p27 enla inducción de la tolerancia de las células T y discutiremos la asociaciónentre pérdida de tolerancia y desarrollo de autoinmunidaden ratones p21/
Over the last few years, evidence has accumulated showingthat cell cycle regulation of T cells is essential to establish toleranceand to suppress autoimmunity. Although apoptosis hasbeen considered an important mechanism in the control of toleranceand autoimmunity development, cell cycle regulation alsoconstitutes a pivotal alternative pathway in the prevention of autoreactivity.Several cell cycle-associated molecules act as autoimmunitysuppressors. The cell cycle inhibitors p21 and p27 haverecently been shown to control T cell tolerance, while p21 also restrainsdevelopment of autoimmunity. In this review, we willexplore the effects of p21 and p27 in T cell tolerance induction anddiscuss the association between tolerance loss and autoimmunitydevelopment in p21/ mice (AU)
Subject(s)
Humans , Cell Cycle/physiology , Autoimmunity/physiology , Homeostasis/physiology , Apoptosis/physiology , T-Lymphocytes/physiology , Gene Products, rex/physiology , Proto-Oncogene Proteins p21(ras)/physiology , Lupus Vulgaris/immunologyABSTRACT
E2Fs are important regulators of proliferation, differentiation, and apoptosis. Here we characterize the phenotype of mice deficient in E2F2. We show that E2F2 is required for immunologic self-tolerance. E2F2(-/-) mice develop late-onset autoimmune features, characterized by widespread inflammatory infiltrates, glomerular immunocomplex deposition, and anti-nuclear antibodies. E2F2-deficient T lymphocytes exhibit enhanced TCR-stimulated proliferation and a lower activation threshold, leading to the accumulation of a population of autoreactive effector/memory T lymphocytes, which appear to be responsible for causing autoimmunity in E2F2-deficient mice. Finally, we provide support for a model to explain E2F2's unexpected role as a suppressor of T lymphocyte proliferation. Rather than functioning as a transcriptional activator, E2F2 appears to function as a transcriptional repressor of genes required for normal S phase entry, particularly E2F1.
Subject(s)
Autoimmune Diseases/genetics , Autoimmunity/immunology , Cell Cycle Proteins , DNA-Binding Proteins , Gene Expression Regulation/immunology , Repressor Proteins/physiology , Self Tolerance/immunology , T-Lymphocytes/cytology , Transcription Factors/physiology , Animals , Apoptosis , Autoimmune Diseases/immunology , Autoimmune Diseases/pathology , Autoimmunity/genetics , Cell Division , Chimera , Clonal Deletion , E2F Transcription Factors , E2F1 Transcription Factor , E2F2 Transcription Factor , Glomerulonephritis, Membranoproliferative/genetics , Glomerulonephritis, Membranoproliferative/immunology , H-Y Antigen/genetics , H-Y Antigen/immunology , Humans , Immunologic Memory , Inflammation , Jurkat Cells , Lymphocyte Activation , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout , Mutagenesis, Site-Directed , Receptors, Antigen, T-Cell/immunology , Recombinant Fusion Proteins/immunology , Repressor Proteins/genetics , S Phase/genetics , Self Tolerance/genetics , Splenomegaly/genetics , Splenomegaly/immunology , T-Lymphocytes/immunology , T-Lymphocytes/pathology , Thymus Gland/immunology , Thymus Gland/pathology , Transcription Factors/biosynthesis , Transcription Factors/deficiency , Transcription Factors/genetics , TransfectionABSTRACT
To trigger an effective immune response, lymphocytes must proliferate. In addition to their direct involvement in cell-cycle progression, cell-cycle regulators might thus control immune functions. Recent evidence suggests that these regulators are essential for T-cell function; we argue that their study will provide clues for dissecting anergy and tolerance mechanisms, as well as for intervention in autoimmune diseases.
Subject(s)
Cell Cycle/immunology , Immunity , Animals , Autoimmunity , Humans , Immune Tolerance , Lymphocyte Activation , Models, BiologicalABSTRACT
Alterations in the cell division:cell death ratio induce multiple autoimmune and transformation processes. Phosphoinositide 3-kinase (PI3K) controls cell division and cell death in vitro, but its effect on the function of the cellular immune system and on tumor formation in mammals is poorly characterized. Here we show that transgenic mice expressing in T lymphocytes an active form of PI3K derived from a thymic lymphoma, p65(PI3K), developed an infiltrating lymphoproliferative disorder and autoimmune renal disease with an increased number of T lymphocytes exhibiting a memory phenotype and reduced apoptosis. This pathology was strikingly similar to that described in mice exhibiting heterozygous loss of the tumor suppressor PTEN, a lipid and protein phosphatase. We show that overexpression of PTEN selectively blocks p65(PI3K)-induced 3T3 fibroblast transformation. Moreover, the early development of T cell lymphomas in p65(PI3K) Tg p53(-/-) mice indicated that PI3K contributes to tumor development. These observations demonstrate that constitutive activation of PI3K extends T cell survival in vivo, affects T cell homeostasis, and contributes to tumor generation, supporting a model in which selective increases in one type of PTEN substrate, the PI3K-derived lipid products, induce tumorigenesis. PI3K thus emerges as a potential target in autoimmune disease and cancer therapy.
Subject(s)
Cell Transformation, Neoplastic/genetics , Lymphoproliferative Disorders/enzymology , Phosphatidylinositol 3-Kinases/metabolism , Tumor Suppressor Proteins , Animals , Autoimmune Diseases/genetics , CD4-Positive T-Lymphocytes/enzymology , CD4-Positive T-Lymphocytes/immunology , CD4-Positive T-Lymphocytes/metabolism , Genes, Tumor Suppressor , Kidney Diseases/genetics , Loss of Heterozygosity , Mice , Mice, Transgenic , PTEN Phosphohydrolase , Phosphatidylinositol 3-Kinases/genetics , Phosphatidylinositols/metabolism , Phosphoric Monoester Hydrolases/genetics , Phosphoric Monoester Hydrolases/metabolismABSTRACT
Here we show that the cell-cycle regulator p21 is involved in immune system function. T lymphocytes from p21-/- mice exhibit significant proliferative advantage over wild-type cells following prolonged stimulation, but not after primary activation. Consistent with this, p21-deficient mice accumulate abnormal amounts of CD4+ memory cells, and develop loss of tolerance towards nuclear antigens. Similar to human lupus, female p21-deficient mice develop antibodies against dsDNA, lymphadenopathy, and glomerulonephritis, leading to decreased viability. These data demonstrate a specialized role for p21 in the control of T-cell proliferation, tolerance to nuclear antigens, and female-prone lupus. These findings could be the basis for new therapeutic approaches to lupus.
Subject(s)
Cell Division/physiology , Cyclins/physiology , Genetic Linkage , Lupus Vulgaris/pathology , T-Lymphocytes/cytology , Animals , Antibodies, Antinuclear/immunology , Cyclin-Dependent Kinase Inhibitor p21 , DNA/immunology , Female , Glomerulonephritis/immunology , Immunologic Memory , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Sex Factors , T-Lymphocytes/immunologyABSTRACT
Although systemic lupus erythematosus appears to be a humorally mediated disease, both Th1 and Th2 type responses have been implicated in its pathogenesis. The Th1 response, as exemplified by IFN-gamma production, has been uniformly shown in mouse lupus models to be critical for disease induction. The role of Th2 type responses, however, is more complicated, with some studies showing detrimental and others beneficial effects of IL-4 in these models. To further address this issue, we generated and analyzed IL-4 gene-deficient BXSB mice. Mice homozygous for this deletion had significantly lower serum levels of total IgG1 compared with wild-type BXSB, consistent with the lack of IL-4. However, no significant differences were observed in mortality, spleen weight, severity of glomerulonephritis, levels of anti-chromatin and anti-ssDNA Abs, or frequency of activated (CD44high) CD4+ T cells. The anti-chromatin Ab isotype response was virtually all Th1 type in both the knockout and wild-type BXSB. These findings directly demonstrate that IL-4 and, by inference, Th2 cells are not obligatory participants in the induction and maintenance of lupus in this strain.
Subject(s)
Interleukin-4/deficiency , Interleukin-4/genetics , Lupus Nephritis/genetics , Lupus Nephritis/immunology , Animals , Autoantibodies/blood , Crosses, Genetic , Glomerulonephritis/genetics , Glomerulonephritis/immunology , Glomerulonephritis/pathology , Immunoglobulin G/biosynthesis , Immunologic Memory/genetics , Interleukin-4/physiology , Lupus Nephritis/mortality , Lupus Nephritis/pathology , Lymphoid Tissue/pathology , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Survival Analysis , T-Lymphocyte Subsets/immunology , T-Lymphocyte Subsets/metabolism , Th2 Cells/immunology , Th2 Cells/metabolismABSTRACT
Imbalances of Th1- and Th2-type responses have been postulated to be a predisposing factor for both humoral and cellular mediated autoimmune diseases. To further define their roles in systemic autoimmunity, IL-4 and IFN-gamma gene knockout mice were studied for susceptibility to the prototypic Th2-mediated mercury-induced autoimmunity. A predominant Th2-type response following HgCl2 treatment of wild-type B10.S mice was confirmed by the findings of a significant increase in splenic IL-4 and hypergammaglobulinemia primarily of the IgG1 isotype, without an increase in IFN-gamma levels. Paradoxically, IL-4-deficient mice developed the characteristic anti-nucleolar autoantibodies and tissue deposition of immune complexes, while IFN-gamma-deficient mice had very low autoantibody levels and essentially normal immunohistology. Studies to define defects in Ab responses of IFN-gamma-deficient mice, using the T-dependent Ag (4-hydroxy-3-nitrophenyl)acetyl, revealed an attenuated IgG response to low and to a lesser extent high doses of (4-hydroxy-3-nitrophenyl)acetyl-hemocyanin, but maintenance of affinity maturation. These results indicate that Th1/Th2 imbalance does not directly play a role in susceptibility to mercury-induced autoimmunity, and suggest that the dependence on Th1-type responses in certain autoimmune diseases is due to the requirement for IFN-gamma for Ab production to weakly antigenic self molecules.
Subject(s)
Autoimmune Diseases/chemically induced , Interferon-gamma/physiology , Mercuric Chloride/immunology , Th1 Cells/immunology , Th2 Cells/immunology , Animals , Autoantibodies/biosynthesis , Autoimmune Diseases/genetics , Autoimmune Diseases/immunology , Disease Susceptibility , Dose-Response Relationship, Immunologic , Haptens/immunology , Interferon-gamma/genetics , Interleukin-4/biosynthesis , Interleukin-4/deficiency , Interleukin-4/genetics , Mice , Mice, Inbred C57BL , Mice, Knockout , Nitrophenols/immunology , Phenylacetates , Th1 Cells/metabolism , Th2 Cells/metabolismABSTRACT
Polypeptide phosphorylation and sialylation of the glycan moieties contribute to the charge heterogeneity of the class I major histocompatibility complex glycoproteins. The present study demonstrates that a unique acidic modification unrelated to phosphorylation or glycosylation also affects the charge heterogeneity of the H2-Kk heavy chain of BW5147 lymphoma cells. In vitro cultivation of BW5147 cells results in changes in charge heterogeneity of the H2-Kk heavy chains due to the unique acidic modification. Sequential papain digestion of the 45 000 Mr H2-Kk glycoprotein yields a 42 500 Mr glycopolypeptide initially, followed by production of a 39 000 Mr glycopolypeptide. Results from experiments designed to localize and characterize the novel acidic modification suggest that the modification resides in the segment of the H2-Kk polypeptide located between the two papain cleavage sites. This portion of the polypeptide consists of the transmembrane region and part of the cytoplasmic domain of the H2-Kk heavy chain. At steady state, 25% of the total cell surface H2-Kk possesses this modification. In addition, the modification is mutually exclusive with the phosphorylation of the H2-Kk heavy chain at Ser-333. The possible biological significance of the novel modification of class I antigens is discussed.
Subject(s)
Cytoplasm/metabolism , H-2 Antigens/chemistry , H-2 Antigens/metabolism , Protein Processing, Post-Translational , Amino Acid Sequence , Animals , Cell Culture Techniques , Electrophoresis, Gel, Two-Dimensional , Glycoproteins/chemistry , Glycoproteins/metabolism , Immunoglobulin Heavy Chains/chemistry , Immunoglobulin Heavy Chains/metabolism , Mice , Molecular Sequence Data , N-Acetylneuraminic Acid/metabolism , Neuraminidase/metabolism , Papain/metabolism , Peptide Fragments/chemistry , Peptide Fragments/metabolism , Phosphorylation , Phosphoserine/metabolism , Protein Isoforms/chemistry , Protein Isoforms/metabolism , Protein Structure, Tertiary , Static Electricity , Time Factors , Tumor Cells, CulturedABSTRACT
Congenic MRL-lpr mice homozygous and heterozygous for the IFN-gamma gene disruption were created to assess the role of this pleotropic cytokine on the lymphoaccumulation and lupus-like disease of Fas-defective mice. Early death was prevented, and glomerulonephritis severely reduced in IFN-gamma-/- mice. Hypergammaglobulinemia was maintained with a switch from IgG2a to IgG1 predominance, but the dramatic decrease in levels of the dominant IgG2a anti-dsDNA autoantibodies was not associated with a compensatory increase in TH2-associated IgG subclasses. Remarkably, early death and glomerulonephritis were also prevented in IFN-gamma+/- mice, although autoantibody levels and glomerular immune deposits were equivalent to IFN-gamma+/+ lpr mice, indicating the importance of additional locally-exerted disease-promoting effects of IFN-gamma. IFN-gamma-/- mice exhibited reduced lymphadenopathy concomitant to a decrease in DN B220(+) T cells. In vivo BrdU labeling showed reduced proliferation of DN B220(+) cells in IFN-gamma-/- vs. IFN-gamma+/+ lpr mice, while enhanced proliferation of all other T cell subsets was unaffected. Macrophages of IFN-gamma-/-lpr mice expressed markedly decreased levels of MHC class I and II molecules compared with controls. Moreover, the heightened expression of MHC class II molecules on proximal tubules of IFN-gamma+/+ lpr mice was significantly reduced in both IFN-gamma-/- and IFN-gamma+/- mice. The data indicate that IFN-gamma hyperproduction is required for lupus development, presumably by increasing MHC expression and autoantigen presentation to otherwise quiescent nontolerant anti-self T cells, and also by promoting local immune and inflammatory processes.
Subject(s)
Interferon-gamma/physiology , Lupus Vulgaris/etiology , Animals , Histocompatibility Antigens Class I/analysis , Histocompatibility Antigens Class II/analysis , Interferon-gamma/analysis , Interferon-gamma/genetics , Lupus Vulgaris/immunology , Lupus Vulgaris/pathology , Lymphatic Diseases/etiology , Lymphocyte Activation , Mice , Mice, Inbred C57BL , Mice, Inbred MRL lpr , T-Lymphocytes/immunologyABSTRACT
To characterize the functional status of lpr T cells and determine whether activation is required for DN cell expansion, we performed in vivo labeling experiments in MRL-+/+, MRL-lpr, and p59fyn-/- MRL-lpr (Fyn-/-) mice. Multicolor FACS analysis of T cells from 8-wk-old mice receiving bromodeoxyuridine (BrdUrd) for 9 days showed that higher proportions of CD4+ and CD8+ lymph node cells were dividing (BrdUrd(high)) in lpr (15%) than in +/+ mice (3%), and the proportion of cycling cells was even higher in the DN (71%) and CD4+ B220+ (54%) lpr subsets. BrdUrd chase experiments documented that activation and division in most DN cells was initiated subsequent to their precursor CD8+ stage. Lymphadenopathy and other disease manifestations were greatly reduced in Fyn-/- lpr mice concomitant to decreased DN cells (from 77 to 20%). BrdUrd chase experiments showed that the division rate, signified by conversion of DN cells from BrdUrd(high) to BrdUrd(low), was severely reduced in Fyn-/- compared with conventional lpr mice, whereas division of all other T cell subsets (CD4+, CD8+, and CD4+ B220+) was equal in both types of mice. We conclude that 1) DN lpr T cells, rather than being end stage, retain the capacity to be activated and repeatedly divide before reaching an anergic or replicative senescence stage; 2) the CD3 zeta-chain-associated Fyn kinase is important to DN T cell signal transduction; 3) DN cells, as functional intermediates of the CD8+ subset, are highly dependent on Fas participation for apoptosis; and 4) DN cells contribute to the early development of the serologic and histologic features of the MRL-lpr lupus disease.
Subject(s)
Autoimmune Diseases/immunology , Lymphoproliferative Disorders/immunology , Proto-Oncogene Proteins/physiology , T-Lymphocyte Subsets/immunology , Animals , Antibodies, Antinuclear/blood , Autoimmune Diseases/genetics , Autoimmune Diseases/pathology , CD4 Antigens/analysis , CD8 Antigens/analysis , Cell Division , Leukocyte Common Antigens/analysis , Lymphocyte Activation , Lymphoid Tissue/immunology , Lymphoid Tissue/pathology , Lymphoproliferative Disorders/genetics , Lymphoproliferative Disorders/pathology , Mice , Mice, Inbred MRL lpr , Models, Immunological , Phosphorylation , Protein Processing, Post-Translational , Proto-Oncogene Proteins c-fyn , fas Receptor/physiologyABSTRACT
Recent studies have documented incomplete TCR V alpha-chain allelic exclusion and dual V alpha-bearing T cells. Herein, we show that V beta allelic exclusion is also incomplete, since a significant proportion of peripheral T cells express dual V beta in both TCR transgenic and normal mice. Studies in TCR transgenic mice indicated that although a small proportion of T cells escaped allelic exclusion in the thymus, dual V beta-expressing cells expanded dramatically in the periphery with age, and such expanded cells had an activated phenotype. Although not as pronounced, age-related increases in dual V beta-bearing cells were also observed in normal mice. These findings may have important implications for TCR selection and specificity, age-related repertoire changes, and autoimmune disease pathogenesis.
Subject(s)
Receptors, Antigen, T-Cell, alpha-beta/genetics , T-Lymphocytes/immunology , Alleles , Animals , Base Sequence , Immunoglobulin Variable Region/genetics , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Transgenic , Molecular Sequence Data , RNA/analysis , Receptors, Antigen, T-Cell, alpha-beta/chemistry , T-Lymphocytes/metabolismABSTRACT
Susceptibility to systemic lupus erythematosus has been unequivocally established to be an inherited trait, but the exact genes and how they confer susceptibility remain largely unknown. In this study of (NZB x NZW)F2 intercross mice, we used linkage analysis of markers covering > 90% of the autosomal genome and identified eight susceptibility loci (Lbw1 to -8, chromosomes 17, 4-7, 18, 1, 11, respectively) associated with antichromatin autoantibody production, glomerulonephritis, and/or mortality. Only one locus, the major histocompatibility complex, was linked to all three traits. Two other loci were associated with both glomerulonephritis and mortality, whereas the remaining loci were linked to one of the above traits. Two additional loci (Sbw1 and -2) that contributed to splenomegaly were also identified. The Sbw2 locus mapped to the identical region as Lbw2, a locus on chromosome 4 linked to glomerulonephritis and mortality, suggesting a single locus with pleiotropic effects. The results indicate that the immunopathologic features of lupus are affected by distinct, but additive, genetic contributions. Studies to determine the nature of the genes associated with these loci should help define the genetic mechanisms involved in this systemic autoimmune disease.