ABSTRACT
Plant plasma membranes (PMs) contain pattern recognition receptors (PRRs), lately believed to be associated within multicomponent complexes, which perceive microbe-associated molecular pattern (MAMP) molecules like lipopolysaccharides (LPSs) and result in signal transduction events that lead to activated immune defense responses. As such, Arabidopsis thaliana leaves were treated with LPS from Escherichia coli (LPSE.coli) over time, and PM fractions isolated and evaluated using gel-based and subsequent mass spectrometry approaches for identification of LPS-responsive proteins. From the identified protein bands and spots, it is concluded that perception of hexaacylated LPS and resulting signal transduction occurs via PM-associated protein(s), amongst others, receptor-like kinases (RLKs) including G-type lectin S-receptor kinase and BAK1, and mostly likely within specialized perception domains.
Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Lipopolysaccharides/pharmacokinetics , Membrane Proteins/metabolism , Proteome/metabolism , Plant Leaves/metabolismABSTRACT
Canis familiaris oral papillomavirus, formerly canine oral papillomavirus, is a causative agent of the self-resolving canine oral papillomatosis and was first described in 1994. This is the first report of two full-length genome sequences described in South Africa and indicates the highly conserved nature of Canis familiaris oral papillomavirus.
