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Surg Infect (Larchmt) ; 19(3): 352-357, 2018 Apr.
Article in English | MEDLINE | ID: mdl-29466092

ABSTRACT

BACKGROUND: Despite improved peri-operative care, prosthetic graft infections continue to cause substantial morbidity and mortality. Contemporary graft infection models have tested a conduit's infectability using varying concentrations without standardization. Using a static assay in vitro model, we sought to evaluate the impact of inoculation concentration on vascular conduit attachment. METHODS: The 2-hour and 24-hour attachment of Staphylococcus aureus TCH1516 and Pseudomonas aeruginosa PA01-UW were determined on polytetrafluoroethylene (PTFE), Dacron®, nitinol, cobalt chromium, and Viabahn® (W.L. Gore and Associates, Newark, DE) endoprotheses. Individually and in combination, concentrations at 104, 105, 106, 107, and 108 were tested on 2-mm sections of each graft. After each time interval, the prosthetics were rinsed to remove non-attached bacteria, sonicated to release the attached bacteria, spiral plated, and then analyzed for the attached concentration. RESULTS: After two hours, the higher initial inoculation concentration translated into a higher attachment percentage, but the mean attachment percentage was only 14.8% in the 108 group. Pseudomonas aeruginosa had the greatest mean attachment across all material and concentration groups. The sequence of attachment on the conduits followed a constant order: Dacron, PTFE, cobalt, nitinol, and Viabahn with no difference between Dacron and PTFE. Although there were still differences at the 24-hour mark, the median attachment at each concentration was greater than the highest initial concentration (108). CONCLUSIONS: Initial attachment percentage is poor consistently regardless of inoculation concentration, however, Staphylococcus aureus and Pseudomonas aeruginosa are still able to achieve full attachment after 24 hours. A concentration of less than 107 should be used in vascular graft infection models to ensure adequate bacterial attachment.


Subject(s)
Bacterial Adhesion , Blood Vessel Prosthesis/microbiology , Models, Biological , Prosthesis-Related Infections , Colony Count, Microbial , Humans , Prosthesis-Related Infections/microbiology , Prosthesis-Related Infections/prevention & control , Pseudomonas aeruginosa/pathogenicity , Staphylococcus aureus/pathogenicity , Time Factors
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