ABSTRACT
A series of novel alkyl amide functionalized trifluoromethyl substituted pyrazolo[3,4-b]pyridine derivatives 5, 6 and 7 were prepared starting from 6-phenyl-4-(trifluoromethyl)-1H-pyrazolo[3,4-b]pyridin-3-amine 3 via selective N-alkylation, followed by reaction with different primary aliphatic amines, cyclic secondary amines or l-amino acids under different set of conditions. All the synthesized compounds 5, 6 and 7 were screened for anticancer activity against four cancer cell lines such as A549-Lung cancer (CCL-185), MCF7-Breast cancer (HTB-22), DU145-Prostate cancer (HTB-81) and HeLa-Cervical cancer (CCL-2). The compounds 5i and 6e are found to have promising bioactivity at micro molar concentration.
Subject(s)
Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Pyrazoles/chemistry , Pyrazoles/pharmacology , Pyridines/chemistry , Pyridines/pharmacology , Alkylation , Amides/chemistry , Antineoplastic Agents/chemical synthesis , Cell Line, Tumor , Halogenation , Humans , Neoplasms/drug therapy , Pyrazoles/chemical synthesis , Pyridines/chemical synthesis , Structure-Activity RelationshipABSTRACT
Tuberculosis is at the present time a worldwide endemic disease. Extrapulmonary tuberculosis is less frequent than the pulmonary form and its diagnosis represents a challenge. Laryngeal tuberculosis accounts for less than 1 percent of extrapulmonary tuberculosis. In this report, we present a case of laryngeal tuberculosis diagnosed in a 12 years old girl at the pediatric department of a general hospital. The patient had a six months history of hoarseness and persistent cough. At the time she was admitted at the hospital she complainted about stridor, odynophagia, weakness and respiratory distress. A cervical mass was the main finding at her physical exam. A laryngeal nodule was shown after practicing a direct laryngoscopy. The biopsy of this lesion revealed presence of a granulomatous inflammatory process compatible with tuberculosis. Besides the samples of sputum taken at her admission for acid -fast bacilli direct examination and culture were positive. We would like to emphasize the delay in diagnosing this case, because of the outpatient clinical study failed in proposing to carry out a direct laryngoscopy in this patient, despite she complainted of hoarseness and persistent cough.
La tuberculosis (TBC) es en la actualidad una endemia a nivel mundial. La tuberculosis extrapulmonar, menos frecuente que la forma pulmonar constituye un desafío diagnóstico. La forma laríngea representa menos del 1 por ciento de las tuberculosis extrapulmonares. En esta publicación, presentamos un caso de tuberculosis laríngea diagnosticada en una escolar de nuestro servicio de pediatría hospital Dr. Gustavo Fricke, cuyos síntomas cardinales fueron disfonía y tos presentes durante 6 meses previo al diagnóstico. Al momento de ingreso al servicio se había agregado compromiso del estado general, odinofagia estridor y dificultad respiratoria. Al examen físico destacaba la presencia de masa cervical. El diagnóstico se realizó mediante laringoscopia directa que evidenció nódulo en cuerda vocal derecha cuya biopsia reveló presencia de proceso inflamatorio crónico granulomatoso compatible con TBC. Las baciloscopías y cultivo de Koch de expectoración, realizados al momento del ingreso, resultaron positivos. Cabe destacar el retraso diagnóstico del caso, debido a que nunca se planteó realizar laringoscopia durante el estudio ambulatorio, pese a la presencia de disfonía y tos persistente.
Subject(s)
Humans , Female , Child , Laryngoscopy/methods , Tuberculosis, Laryngeal/diagnosis , Radiography, Thoracic , Tomography, X-Ray Computed , Cough/etiology , Tuberculosis, Laryngeal/complicationsABSTRACT
In this study we compared the sensitivity of molecular, serologic and parasitologic methods for diagnosis of equine trichinellosis in two abattoirs, one rural and one federal inspection type. Diaphragm muscle samples were obtained from 170 slaughter horses and examined by artificial digestion and PCR. Serum samples from these horses were also analyzed by ELISA. No Trichinella muscle larvae were detected by artificial digestion. However, specific antibodies against Trichinella were detected in 17% and 7% of the serum samples examined from the rural and the federal abattoirs respectively. By PCR, 15% and 2% of the samples from these two abattoirs were Trichinella positive.
Subject(s)
Horse Diseases/diagnosis , Muscle, Skeletal/parasitology , Trichinellosis/veterinary , Abattoirs , Animals , DNA, Helminth/isolation & purification , Enzyme-Linked Immunosorbent Assay/methods , Horse Diseases/parasitology , Horses , Polymerase Chain Reaction/methods , Reproducibility of Results , Trichinella/isolation & purification , Trichinellosis/diagnosisABSTRACT
En un trabajo de gestión coordinada de un equipo de salud hospitalario, se presenta la experiencia del equipo directivo del Hospital Gustavo Fricke, establecimiento terciario y centro de referencia del Servicio de Salud Viña del Mar-Quillota, en la organización y desarrollo de la denominada Campaña de Invierno, programa destinado a enfrentar la crisis de sobredemanda de atención causada por las emmergencias respiratorias infantiles durante el invierno de 1998. Se describe en detalle el método y específicamente las fuerzas de tarea que son necesarias para llevar a cabo dicho programa y sus resultados
