ABSTRACT
ABSTRACT Canine distemper causes demyelinating leucoencephalitis, like human multiple sclerosis. The encephalic microenvironment, including the extracellular matrix, is an important key factor of this lesion, already described in multiple sclerosis but not proved in canine distemper. Thereby, the aim of this work is to characterize the extracellular matrix in the encephalon of dogs with canine distemper. Samples of cortex and cerebellum of 14 naturally infected dogs with canine distemper virus were collected after being sent for necropsy in the Animal Pathology Laboratory of the Veterinary Hospital of Uberlândia Federal University. The samples were processed as routine, stained with Hematoxylin and Eosin (H.E), Masson Trichrome (MT), Periodic Acid-Schiff (PAS) and Reticulin, and then described. Areas of demyelination and necrosis were quantified in percentage of stain. The TM samples showed blue stain around vessels and meninge, which indicates a higher deposition of collagen in lesioned areas. At necrotic areas, reticulin stain pointed to a disorganization in the vascular wall and PAS-stained pink granules in macrophages. We conclude that the extracellular matrix seems to participate in the pathogeny of canine distemper. More research should be done to better detail the involvement of these molecules in the course of this disease.
RESUMO A cinomose canina, assim como a esclerose múltipla, provoca leucoencefalite desmeilinizante. O microambiente encefálico, incluindo a matriz extracelular, atua como fator adjuvante na esclerose múltipla, porém não há comprovação do mesmo na cinomose canina. Portanto o objetivo neste trabalho é caracterizar a matriz extracelular do encéfalo de cães com cinomose canina. Foram coletados fragmentos de córtex frontal e cerebelo de 14 cães naturalmente infectados com cinomose canina encaminhados para necropsia no Laboratório de Patologia Animal do Hospital Veterinário da Universidade Federal de Uberlândia. Os fragmentos foram processados rotineiramente, corados com Hematoxilina e Eosina (H.E.), Tricrômico de Masson (TM), Ácido-Periódico de Schiff (PAS) e Reticulina e então foram descritas. As áreas com desmielinização e/ou necrose também foram quantificadas. A coloração de TM evidenciou marcação azulada em vasos e meninge, indicando maior deposição de fibras colágenas nas lesões. Nas áreas de necrose notou-se parede vascular desorganizada, a partir da reticulina, além de pigmentos róseos em macrófagos corados com PAS. Conclui-se que a matriz extracelular aparenta participar na patogenia da cinomose. Outros estudos são necessários para maiores detalhes da ação dessas moléculas na patogenia da cinomose canina.
ABSTRACT
Objetivou-se neste estudo descrever a técnica de xenotransplante gonadal em camundongosimunossuprimidos por meio de ovariectomia unilateral total videolaparoscópica (OVL) em ovelhas. Foramutilizadas quatro ovelhas como doadoras do tecido reprodutivo e camundongos em fase reprodutiva (n = 12)como receptores. As coletas foram realizadas por videocirurgia, e foram avaliados o tempo de cirurgia, as respostascomportamentais antiálgicas, o apetite, o escore corporal e a locomoção durante 10 dias pós-operatórios. Osxenotransplantes foram realizados na cápsula renal dos camundongos, sendo avaliados histologicamente 60 diasapós o transplante. O tempo de cirurgia foi de 18 ± 3 min. A técnica OVL apresentou-se minimamente invasiva,sem complicações cirúrgicas, proporcionando mínimo desconforto e ótima recuperação dos animais, e pode serempregada em ovelhas adultas e pré-púberes para coleta de tecidos reprodutivos para conservação de gametas. Parahistologia dos tecidos xenotransplantados, verificou-se viabilidade tecidual, com neovascularização e ausência defocos necróticos, porém não se visualizaram folículos nas diferentes fases. Concluiu-se que a OVL pode ser atécnica empregada em ovelhas pré-púberes e adultas para recuperação de tecido reprodutivo paraxenotransplante. Para o xenotransplante, sugere-se que os fragmentos ovarianos selecionados contenham umnúmero suficiente de folículos primordiais para o possível sucesso da biotécnica.(AU)
The aim of this study was to describe the technique of gonadal xenotransplantation inimmunosuppressed mice from total unilateral laparoscopic ovariectomy (OVL) in sheep. Four sheep were usedas donors of reproductive tissue in mice in the reproductive phase (n = 12) as receivers. Samples were collectedby laparoscopic surgery, evaluating the time of surgery, observation of behavioral anti-painful responses,monitoring appetite, body condition score and locomotion for 10 days postoperatively. The xenografts wereperformed in the renal capsule of mice, as evaluated histologically 60 days after transplantation. The operationtime was 18 ± 3 min, the technique presented OVL minimally invasive surgical complications without providingminimum discomfort and optimum recovery of the animals and can be used in adult and prepubertal sheep forcollecting reproductive tissues for the conservation of gametes. Histology tissue xenotransplanteted was a viabletissue, with neovascularization and absence of necrotic foci, but follicles at different stages were not visualized.It was concluded that the OVL technique can be employed in sheep and adult prepubertal for recoveringreproductive tissue for xenotransplantation. For xenotransplantation, it is suggested that the ovarian fragmentsselected contain a sufficient number of primordial follicles for the possible success of biotech.(AU)
Subject(s)
Animals , Female , Sheep , Transplantation, Heterologous , Mice , OvariectomyABSTRACT
Objetivou-se neste estudo descrever a técnica de xenotransplante gonadal em camundongosimunossuprimidos por meio de ovariectomia unilateral total videolaparoscópica (OVL) em ovelhas. Foramutilizadas quatro ovelhas como doadoras do tecido reprodutivo e camundongos em fase reprodutiva (n = 12)como receptores. As coletas foram realizadas por videocirurgia, e foram avaliados o tempo de cirurgia, as respostascomportamentais antiálgicas, o apetite, o escore corporal e a locomoção durante 10 dias pós-operatórios. Osxenotransplantes foram realizados na cápsula renal dos camundongos, sendo avaliados histologicamente 60 diasapós o transplante. O tempo de cirurgia foi de 18 ± 3 min. A técnica OVL apresentou-se minimamente invasiva,sem complicações cirúrgicas, proporcionando mínimo desconforto e ótima recuperação dos animais, e pode serempregada em ovelhas adultas e pré-púberes para coleta de tecidos reprodutivos para conservação de gametas. Parahistologia dos tecidos xenotransplantados, verificou-se viabilidade tecidual, com neovascularização e ausência defocos necróticos, porém não se visualizaram folículos nas diferentes fases. Concluiu-se que a OVL pode ser atécnica empregada em ovelhas pré-púberes e adultas para recuperação de tecido reprodutivo paraxenotransplante. Para o xenotransplante, sugere-se que os fragmentos ovarianos selecionados contenham umnúmero suficiente de folículos primordiais para o possível sucesso da biotécnica.
The aim of this study was to describe the technique of gonadal xenotransplantation inimmunosuppressed mice from total unilateral laparoscopic ovariectomy (OVL) in sheep. Four sheep were usedas donors of reproductive tissue in mice in the reproductive phase (n = 12) as receivers. Samples were collectedby laparoscopic surgery, evaluating the time of surgery, observation of behavioral anti-painful responses,monitoring appetite, body condition score and locomotion for 10 days postoperatively. The xenografts wereperformed in the renal capsule of mice, as evaluated histologically 60 days after transplantation. The operationtime was 18 ± 3 min, the technique presented OVL minimally invasive surgical complications without providingminimum discomfort and optimum recovery of the animals and can be used in adult and prepubertal sheep forcollecting reproductive tissues for the conservation of gametes. Histology tissue xenotransplanteted was a viabletissue, with neovascularization and absence of necrotic foci, but follicles at different stages were not visualized.It was concluded that the OVL technique can be employed in sheep and adult prepubertal for recoveringreproductive tissue for xenotransplantation. For xenotransplantation, it is suggested that the ovarian fragmentsselected contain a sufficient number of primordial follicles for the possible success of biotech.
Subject(s)
Female , Animals , Mice , Ovariectomy , Sheep , Transplantation, HeterologousABSTRACT
Gross and microscopic evaluations of the ovaries of sheep subjected to successive ovum collection were performed with the objective of identifying if there was interference in oocyte production and morphology of gonads. Gross evaluation of the internal genital tract was also performed. Eighteen ewes of the Santa Inês breed were randomly distributed into three experimental groups of six animals each; G0, G1 and G9 with no, one and nine repetitions, respectively. Estrous synchronization was achieved with a short protocol using MAP followed by single dose stimulation with 80mg of FSHp and 300 IU of eCG (IM). Laparoscopic ovum pick-up was performed 36h later, with 7-day intervals. The number of visualized follicles, aspirated follicles and oocytes recovery was recorded. After the last intervention, ovariectomy was performed for evaluation of gross and microscopical appearance and existence of lesions caused by follicular puncture, which were classified as absent (0), mild (1), moderate (2) and severe (3). The number of visualized follicles, aspirated follicles and oocytes recovery were 13.2±2.0, 11.3±3.0 and 5.8±2.3, respectively, with a recovery rate of 51.7%. No statistical difference was found between the nine sessions (P>0.05). Nine sessions of superovulation and ovum collection procedures did not cause ovarian lesions and did not interfere with the production of follicles in ewes of the Santa Inês breed.