ABSTRACT
Antecedentes y objetivo: En México y en el mundo cada vez son más frecuentes los casos de cáncer de cérvix y aunque se sabe que existen diversos factores de riesgo para padecer este tipo de cáncer, se ha sugerido que las alteraciones en el microbioma cervical pueden desempeñar un papel importante en el establecimiento y progresión del cáncer, específicamente hablando de la presencia de aquellas bacterias asociadas a vaginosis bacteriana como lo son Atopobium vaginae y Gardnerella vaginalis. Material y métodos: Se estudió el ADN de un total de 177 muestras de raspado de cérvix, de las cuales 104 pertenecían a mujeres sin alteraciones citológicas o colposcópicas y 73 muestras de lesiones precursoras con antecedente de infección por virus del papiloma humano (VPH). Todas las muestras fueron sometidas a detección de secuencias de Atopobium vaginae, Gardnerella vaginalis y VPH por medio de PCR. Resultados: El VPH fue encontrado en mayor proporción en las muestras precursoras, y poco más del 30% de las muestras sin lesión fueron positivas para VPH. La mayoría de las muestras contienen secuencias de ambas bacterias sin asociación con la presencia viral, lo que podría indicar que estos microorganismos serían parte del microbioma cervical en población mexicana. Conclusiones: Los resultados obtenidos indican que las bacterias analizadas potencialmente forman parte del bioma normal en las mujeres mexicanas por lo que es de especial importancia reconsiderar el papel patógeno de estos microorganismos
Background and objective: Cervical cancer is an important health problem in our country. It is known that there are several risk factors for this neoplasm, and it has been suggested that cervical microbiome alterations could play a role in the development and progress of cancer. Bacterial vaginosis associated bacteria such as Atopobium vaginae and Gardnerella vaginalis has been suggested as potential risk factor for cervical lesions and cervical cancer. Material and methods: DNA from 177 cervical scraping samples was studied: 104 belonged to women without cytological or colposcopic alterations and 73 samples from precursor lesions with previous human papillomavirus (HPV) infection history. All samples were screened for Atopobium vaginae, Gardnerella vaginalis and HPV by PCR. Results: High HPV prevalence was found in precursor samples, and 30% of samples without lesions were positive for HPV. Virtually all samples contained sequences of both bacteria, and interestingly, there was not HPV association observed; these results could suggest that these microorganisms could be part of the cervical microbiome in Mexican population. Conclusions: The results obtained indicate that the bacteria analysed could be part of normal biome in Mexican women, suggesting a potential reconsideration of the pathogen role of these microorganisms
Subject(s)
Humans , Female , Vaginosis, Bacterial/diagnosis , Papillomavirus Infections/complications , Bacteria/isolation & purification , Vagina/microbiology , Vaginosis, Bacterial/epidemiology , Gardnerella vaginalis/cytology , Gardnerella vaginalis/isolation & purification , Polymerase Chain Reaction/methods , Cross-Sectional Studies , Uterine Cervical Neoplasms/epidemiologyABSTRACT
BACKGROUND AND OBJECTIVE: Cervical cancer is an important health problem in our country. It is known that there are several risk factors for this neoplasm, and it has been suggested that cervical microbiome alterations could play a role in the development and progress of cancer. Bacterial vaginosis associated bacteria such as Atopobium vaginae and Gardnerella vaginalis has been suggested as potential risk factor for cervical lesions and cervical cancer. MATERIAL AND METHODS: DNA from 177 cervical scraping samples was studied: 104 belonged to women without cytological or colposcopic alterations and 73 samples from precursor lesions with previous human papillomavirus (HPV) infection history. All samples were screened for Atopobium vaginae, Gardnerella vaginalis and HPV by PCR. RESULTS: High HPV prevalence was found in precursor samples, and 30% of samples without lesions were positive for HPV. Virtually all samples contained sequences of both bacteria, and interestingly, there was not HPV association observed; these results could suggest that these microorganisms could be part of the cervical microbiome in Mexican population. CONCLUSIONS: The results obtained indicate that the bacteria analysed could be part of normal biome in Mexican women, suggesting a potential reconsideration of the pathogen role of these microorganisms.
