Polymorphism of growth hormone (GH) gene and its association with performance and body conformation of Harnali sheep.

The present study was carried out to study the polymorphism in the GH gene and its association with various performance and body conformation traits, viz., birth weight (B-WT), weaning weight (W-WT), six-month body weight (6 M-WT), one-year body weight (Y-WT), annual greasy fleece weight (AGFW), body length (BL), body height (BH), heart girth (HG) and paunch girth (PG) in 138 Harnali sheep. PCR-RFLP was performed to identify polymorphism in the targeted region of the GH gene. The PCR product of 422 bp size of the GH gene was amplified encompassing partial exon 2 and inton 3 in Harnali sheep. The PCR product was digested with HaeIII restriction enzyme for the detection of Single nucleotide polymorphism (SNP). The digested products revealed the presence of two genotypes, i.e. AA and AB in the studied population. A > G mutation (A781G) was observed in our resource population. The AA genotype was found to be the predominant genotype (0.62). Chi square value revealed that resource population was not under Hardy-Weinberg equilibrium with respect to target locus. Period of birth was found to have significant effect on W-WT, Y-WT, BL, BH and PG. Sex of animal was found to have significant (P < 0.05) effect on W-WT and highly significant (P < 0.01) effect on 6 M-WT, Y-WT and AGFW in Harnali sheep. The effect of genotype was found to be significant (P < 0.05) on annual greasy fleece weight. AB genotype was found to be associated with higher annual greasy fleece weight and can be used as a potential candidate marker in selection criteria for improving greasy fleece weight in Harnali sheep.

Temporal variation and risk factors associated with reproductive disorders in Hardhenu (Bos taurus × Bos indicus) dairy cattle.

This study aimed to investigate the incidence and effect of non-genetic factors on reproductive disorders in the Hardhenu cattle population over a period of 11 years (2010-2021). The study included a total of 1340 cattle for calculating the incidence of reproductive disorders and analysed the occurrence of different reproductive disorders. In the present study, retention of placenta had highest frequency, accounting for approximately 18.36% of the incidence, followed by repeater cows (11.19%), metritis (6.27%), dystocia (0.60%), anestrus (0.45%) and prolapse (0.37%). Chi-square analysis revealed a significant relationship between the year and the incidence of reproductive disorders (p < .05). Logistic regression analysis of temporal variability of reproductive disorders was also analysed, with an increase in the odds from 2014 to 2020, peaking in 2017-2018 and 2018-2019. Logistic regression analysis showed that season and parity did not significantly influence the incidence of reproductive disorders. However, period of calving had a significant impact. Cows calving between 2008 and 2014 had a significantly lower odds ratio (0.13, 95% CI: 0.06-0.30), indicating a decreased risk, while those calving between 2014 and 2017 had a higher odds ratio (0.59, 95% CI: 0.38-0.90), suggesting an increased risk of reproductive disorders. Compared to parity 3, parity 1 (1.344, 95% CI: 0.869-2.08) and parity 2 (1.193, 95% CI: 0.768-1.851) showed highest odds ratio. Similarly, the odds ratio for winter (1.64, 95% CI: 0.93-2.89), summer (1.71, 95% CI: 0.89-3.26) and rainy season (1.64, 95% CI: 0.92-2.91) was higher as compared to autumn. The present results provided valuable insights into the incidence of various reproductive disorders and associated risk factors in Hardhenu cattle. These results can be utilized for targeted interventions to enhance reproductive health and overall productivity in dairy cattle.

Association of CXCR1 gene polymorphism with clinical mastitis, reproductive disorders and performance traits in Hardhenu (Bos taurus × Bos indicus) cattle.

The present study investigated the presence of CXCR1 gene polymorphisms and their association with clinical mastitis, reproductive disorders and performance traits in Hardhenu cattle. Genotyping of the targeted SNP rs211042414 (C>T) at the g.106216468 loci of the CXCR1 gene was performed through PCR amplification and Bsa1 restriction enzyme digestion. The genotypic frequencies revealed three genotypes: CC, CT and TT, with the C allele being the most prevalent. Significant associations were found between the targeted SNP and clinical mastitis occurrence using chi-square and logistic regression analyses. The CC genotype showed higher susceptibility to clinical mastitis with a higher odds ratio of 3.47 compared to TT (1.00) and CT (2.90) genotypes (p < .05). Furthermore, least squares analysis revealed significant associations between genotypes and performance traits such as total milk yield, 305-day milk yield and peak yield (p < .05). The CC genotype exhibited higher milk yields than CT and TT genotypes, indicating a positive association between the C allele and increased milk production. These findings have practical implications for the genetic improvement of Hardhenu cattle. Incorporating the identified CXCR1 gene polymorphisms into existing selection criteria can help enhance disease resistance and milk production traits. However, further validation with a larger sample size is necessary to strengthen the observed associations and ensure their practical applicability.

Association between age at first calving, first lactation traits and lifetime productivity in Murrah buffaloes.

OBJECTIVE: This study was conducted to estimate the association of age at first calving (AFC) with first lactation traits as well as lifetime performance traits in Murrah buffaloes. METHODS: Data on first lactation and life time performance of Murrah buffaloes (n = 679), maintained at Indian Council of Agricultural Research-Central Institute for Research on Buffaloes, Hisar, India during the period 1983 through 2017, were deduced to calculate heritability estimates, genetic and phenotypic correlation of different first lactation and lifetime traits. The univariate animal model was fitted to estimate variance components and heritability separately for each trait, while bivariate animal models were set to estimate genetic and phenotypic correlations between traits under study. RESULTS: The heritability was high for first peak milk yield (FPY, 0.64±0.08), moderate for AFC (0.48±0.07) and breeding efficiency (BE 0.39±0.09). High genetic correlations of first lactation total milk yield (FLTMY) with first lactation standard milk yield (FLSMY, 305 days or less), FPY, and first lactation length (FLL) was seen. Likewise, genetic correlation of AFC was positive with FLTMY, FLL, first dry period (FDP), first service period (FSP), first calving interval (FCI), herd life (HL) and productive days (PD). Significant phenotypic correlation of FLTMY was observed with HL, productive life (PL), PD, total lifetime milk yield (LTMY), standard lifetime milk yield (standard LTMY). Moreover, positive genetic and phenotypic correlation of FPY was observed with HL, PL, PD, total LTMY and standard LTMY. CONCLUSION: This study reports that AFC had positive genetic correlation with FDP, FSP, FCI, and unproductive days while, negative association of AFC was observed with FLSMY, PL, total LTMY, standard LTMY, and BE. This suggests that reduction of AFC would results in improvement of lifetime performance traits.

Evaluation of candidate point mutation of Kisspeptin 1 gene associated with litter size in Indian Goat breeds and its effect on transcription factor binding sites.

Kisspeptin gene (Kiss1) has a significant role in reproductive processes in mammals. However, only little information is available about the association of Kiss1 gene with litter size in Indian goat breeds. Thus, blood samples from 285 randomly selected animals were collected for DNA isolation and SNP profiling. The PCR product of 242 bp size harboring g.2540C>T mutation of Kiss1 gene was digested with the restriction enzyme Sac1. Least squares analysis revealed that Barbari goats showed significantly higher average litter size (2.86±0.08) compared to Beetal, Sirohi and Sojat breeds (P < 0.01). SNP locus g.2540C>T of Kiss1 gene also showed significant effect on litter size (P < 0.01). Goats with Genotype CT (2.66 ± 0.07) and TT (2.67 ± 0.26) had significantly higher (P < 0.01) litter size than CC (1.50 ± 0.05). From the transcription factor binding site analysis, it was predicted that due to g.2540C>T SNP, both native and mutant variant forms coded for putative binding sites for different transcription Factor. Allele T had putative binding sites for the androgen receptor which plays a significant role in the signaling pathway involved in increase in ovulation rate; which consequently can have a tremendous effect on average litter size.

Genotype of MTNR1A gene regulates the conception rate following melatonin treatment in water buffalo.

Buffaloes have tendency to show seasonal reproduction and remain in anestrus due to limited ovarian activity during summer. The seasonal reproductive behavior is ascribed the effect of melatonin related to photoperiod. Treating animals with melatonin could be a possible strategy to overcome the problem. The role of MTNR1A gene has not been fully explained in the buffalo. Therefore, we conducted a study on 114 buffalo heifers to detect the polymorphic site in MTNR1A gene and further treated them with melatonin implants to investigate the role of most frequent genotype following melatonin treatment on pregnancy. The present investigation is the first to investigate the association between melatonin treated different MTNR1A genotype buffalo and pregnancy. We confirmed SNP at position 72 in 812 bp fragment exon II of MTNR1A gene. RFLP of PCR products with Hpa I enzyme resulted in three genotypes: TT (812bp), CT (812, 743, 69bp) and CC (743, 69bp). Next, buffaloes of each genotype (TT, CC, CT; n = 28 for each) were treated with melatonin implants to compare the conception rate with their corresponding untreated control (n = 10 for each genotype). Melatonin concentrations were higher (P < 0.05) for the treatment groups of all genotypes compared to their respective untreated control from day 1-28. The pregnancy rate was significantly associated with the MTNR1A genotype. The conception rate was higher (P < 0.05) for TT genotype than for the other genotypes of buffaloes treated with melatonin. Furthermore, buffaloes of TT genotype treated with melatonin started exhibiting estrus activity soon from second week of melatonin treatment (14.1 ±â€¯2.1; range: 10-17 days) and were found to be 7.8 times more likely to become pregnant compared to other genotypes following melatonin treatment. In conclusion, TT genotype of MTNR1A gene is more sensitive to melatonin treatment that favours pregnancy in buffaloes during summer.

Livestock vaccination in India: an analysis of theory and practice among multiple stakeholders.

An effective and efficient disease prevention and control strategy is of paramount importance to improve the quality and quantity of livestock production in the Indian context. Although livestock vaccination is considered an emerging innovation of socioeconomic importance in the Indian dairy industry, the rate of adoption and diffusion of vaccination technology is very low at field level. In this context, the authors examined the efforts of the Government of India to protect livestock health and control disease, considered the lessons learnt from rinderpest eradication, looked at field practices and the reality on the ground, and studied the perceptions of multiple stakeholders with regards to the relevance, profitability and sustainability of vaccination. In this study, the authors consider policy implications for the Indian dairy industry using the responses of 360 dairy farmers, 80 research scientists and 40 extension workers in India. The study revealed that scientists and extension experts rated vaccination highly in terms of its relevance, profitability and sustainability, while the perception of farmers was less favourable. The study also observed that, even after implementation of various disease control programmes with heavy financial expenditure, there was a wide gap between farmers and scientists and between farmers and extension workers with regards to their perceptions of the relevance, profitability and sustainability of vaccination, while the gap was very narrow between scientists and extension workers. It can be concluded, therefore, that there is a need to generate innovations for disease control that are perceived as relevant, profitable and sustainable so as to encourage higher levels of diffusion and adoption at field level. This study recommends that farmers partner with researchers and extension workers to ensure effective generation and transfer of new dairying technologies, leading to higher production and productivity in the Indian dairy sector.

En Inde, l'amélioration qualitative et quantitative de la productivité des élevages passe nécessairement par la mise en place de stratégies efficaces et efficientes de prévention et de contrôle des maladies animales. Les producteurs de lait et de produits laitiers considèrent la vaccination du bétail comme une innovation importante au plan socio-économique mais le niveau d'adoption et de diffusion des technologies vaccinales demeure très faible sur le terrain. Ce constat a incité les auteurs à effectuer une étude visant à analyser les efforts déployés par le gouvernement indien pour protéger la santé du bétail et pour lutter contre les maladies, à déterminer le niveau de prise en compte des leçons de l'éradication de la peste bovine, et à décrire les pratiques et la situation concrète sur le terrain ainsi que la manière dont les diverses parties prenantes perçoivent la pertinence, la rentabilité économique et les effets dans le temps de la vaccination. Dans cette étude, les auteurs ont interrogé 360 éleveurs de vaches laitières, 80 chercheurs et 40 agents de vulgarisation en Inde afin de déterminer les effets des politiques zoosanitaires sur le secteur de l'élevage laitier indien. L'étude a montré que la pertinence, la rentabilité économique et les effets dans le temps de la vaccination étaient perçus très favorablement par les chercheurs et par les agents de vulgarisation, tandis que la perception des éleveurs était moins positive. L'étude a également permis de constater que l'écart manifeste entre la perception des éleveurs d'un côté et celle des chercheurs et des agents de vulgarisation de l'autre concernant la pertinence, la rentabilité et l'effet dans le temps de la vaccination subsistait même après la mise en oeuvre à grands frais de programmes de lutte contre les maladies animales, tandis que cet écart était minime entre les chercheurs et les agents de vulgarisation. Les auteurs concluent sur la nécessité de veiller à ce que les innovations mises en place en matière de lutte contre les maladies animales soient perçues comme pertinentes, rentables et durables, afin de promouvoir une meilleure appropriation et diffusion de ces méthodes sur le terrain. Les auteurs préconisent le recours à des partenariats entre les éleveurs, les chercheurs et les agents de vulgarisation afin d'assurer une conception et un transfert efficaces des technologies dans le secteur de la production de lait et d'améliorer ainsi la production et la productivité du secteur laitier indien.

En el contexto de la India, para mejorar la producción bovina tanto en calidad como en cantidad es de capital importancia disponer de una estrategia eficaz y eficiente de prevención y control de enfermedades. Aunque la vacunación del ganado está considerada una innovación incipiente de gran importancia socioeconómica para el sector lechero del país, sobre el terreno se dan índices muy bajos de adopción y difusión de las técnicas de vacunación. En tales circunstancias, los autores examinaron las iniciativas del Gobierno de la India para proteger la salud del ganado y combatir sus enfermedades, repasaron las enseñanzas extraídas de la erradicación de la peste bovina, observaron la praxis y las realidades sobre el terreno e indagaron en la percepción que tienen numerosos interlocutores del sector de la pertinencia, rentabilidad y sostenibilidad de las vacunaciones. A partir de las respuestas aportadas por 360 productores, 80 investigadores y 40 agentes de extensión del país, los autores estudian las repercusiones normativas para la industria lechera india. El proceso puso de manifiesto que los científicos y especialistas en extensión otorgan a la vacunación un lugar de privilegio por lo que respecta a su pertinencia, rentabilidad y sostenibilidad, mientras que a los productores les merece una opinión menos positiva. Los autores observaron igualmente que, aun tras la implantación de diversos programas de lucha zoosanitaria que supusieron cuantiosas inversiones económicas, existía un gran desfase entre los productores, por un lado, y los científicos y agentes de extensión, por el otro, en cuanto a la valoración que hacían de la pertinencia, rentabilidad y sostenibilidad de las vacunaciones, mientras que las opiniones de científicos y agentes de extensión apenas diferían. Cabe concluir, por lo tanto, que para inducir sobre el terreno mayores niveles de adopción y difusión es indispensable generar innovaciones de lucha zoosanitaria que sean percibidas como algo pertinente, rentable y sostenible. Los autores recomiendan que los productores trabajen en asociación con los investigadores y agentes de extensión para lograr así que se generen y transfieran eficazmente nuevas tecnologías de producción lechera, lo que a su vez redundará en un aumento de la producción y productividad del sector lechero indio.