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1.
Clin Exp Immunol ; 192(3): 284-291, 2018 06.
Article in English | MEDLINE | ID: mdl-29878323

ABSTRACT

This is the second report of the United Kingdom Primary Immunodeficiency (UKPID) registry. The registry will be a decade old in 2018 and, as of August 2017, had recruited 4758 patients encompassing 97% of immunology centres within the United Kingdom. This represents a doubling of recruitment into the registry since we reported on 2229 patients included in our first report of 2013. Minimum PID prevalence in the United Kingdom is currently 5·90/100 000 and an average incidence of PID between 1980 and 2000 of 7·6 cases per 100 000 UK live births. Data are presented on the frequency of diseases recorded, disease prevalence, diagnostic delay and treatment modality, including haematopoietic stem cell transplantation (HSCT) and gene therapy. The registry provides valuable information to clinicians, researchers, service commissioners and industry alike on PID within the United Kingdom, which may not otherwise be available without the existence of a well-established registry.


Subject(s)
Epidemiological Monitoring , Immunologic Deficiency Syndromes/epidemiology , Registries/statistics & numerical data , Female , Humans , Immunologic Deficiency Syndromes/immunology , Immunologic Deficiency Syndromes/therapy , Male , United Kingdom/epidemiology
2.
Clin Exp Immunol ; 191(2): 212-219, 2018 02.
Article in English | MEDLINE | ID: mdl-28990652

ABSTRACT

Immunoglobulin replacement therapy enhances survival and reduces infection risk in patients with agammaglobulinaemia. We hypothesized that despite regular immunoglobulin therapy, some patients will experience ongoing respiratory infections and develop progressive bronchiectasis with deteriorating lung function. One hundred and thirty-nine (70%) of 199 patients aged 1-80 years from nine cities in the United Kingdom with agammaglobulinaemia currently listed on the UK Primary Immune Deficiency (UKPID) registry were recruited into this retrospective case study and their clinical and laboratory features analysed; 94% were male, 78% of whom had Bruton tyrosine kinase (BTK) gene mutations. All patients were on immunoglobulin replacement therapy and 52% had commenced therapy by the time they were 2 years old. Sixty per cent were also taking prophylactic oral antibiotics; 56% of patients had radiological evidence of bronchiectasis, which developed between the ages of 7 and 45 years. Multivariate analysis showed that three factors were associated significantly with bronchiectasis: reaching 18 years old [relative risk (RR) = 14·2, 95% confidence interval (CI) = 2·7-74·6], history of pneumonia (RR = 3·9, 95% CI = 1·1-13·8) and intravenous immunoglobulin (IVIG) rather than subcutaneous immunoglobulin (SCIG) = (RR = 3·5, 95% CI = 1·2-10·1), while starting immunoglobulin replacement after reaching 2 years of age, gender and recent serum IgG concentration were not associated significantly. Independent of age, patients with bronchiectasis had significantly poorer lung function [predicted forced expiratory volume in 1 s 74% (50-91)] than those without this complication [92% (84-101)] (P < 0·001). We conclude that despite immunoglobulin replacement therapy, many patients with agammaglobulinaemia can develop chronic lung disease and progressive impairment of lung function.


Subject(s)
Agammaglobulinemia/epidemiology , Bronchiectasis/epidemiology , Immunoglobulins, Intravenous/therapeutic use , Lung/metabolism , Respiratory Tract Infections/epidemiology , Adolescent , Adult , Agammaglobulinemia/therapy , Aged , Aged, 80 and over , Bronchiectasis/therapy , Child , Child, Preschool , Female , Humans , Infant , Lung/pathology , Male , Middle Aged , Respiratory Tract Infections/therapy , United Kingdom , Young Adult
3.
Vox Sang ; 110(3): 282-4, 2016 Apr.
Article in English | MEDLINE | ID: mdl-26529032

ABSTRACT

Surveillance of 75 immunodeficiency patients exposed to UK-sourced immunoglobulin, including batches derived from donors who went on to develop vCJD, has not detected any clinical cases of vCJD, or of asymptomatic infection in 15 patients with available tissue samples of sufficient quality for testing.


Subject(s)
Creutzfeldt-Jakob Syndrome/transmission , Immunoglobulins, Intravenous/administration & dosage , Immunologic Deficiency Syndromes/drug therapy , Adult , Asymptomatic Infections , Blood Donors , Genotype , Humans , Immunologic Deficiency Syndromes/pathology , Polymorphism, Genetic , Prion Proteins/genetics
4.
Clin Exp Immunol ; 184(1): 73-82, 2016 Apr.
Article in English | MEDLINE | ID: mdl-26646609

ABSTRACT

Idiopathic hypogammaglobulinaemia, including common variable immune deficiency (CVID), has a heterogeneous clinical phenotype. This study used data from the national UK Primary Immune Deficiency (UKPID) registry to examine factors associated with adverse outcomes, particularly lung damage and malignancy. A total of 801 adults labelled with idiopathic hypogammaglobulinaemia and CVID aged 18-96 years from 10 UK cities were recruited using the UKPID registry database. Clinical and laboratory data (leucocyte numbers and serum immunoglobulin concentrations) were collated and analysed using uni- and multivariate statistics. Low serum immunoglobulin (Ig)G pre-immunoglobulin replacement therapy was the key factor associated with lower respiratory tract infections (LRTI) and history of LRTI was the main factor associated with bronchiectasis. History of overt LRTI was also associated with a significantly shorter delay in diagnosis and commencing immunoglobulin replacement therapy [5 (range 1-13 years) versus 9 (range 2-24) years]. Patients with bronchiectasis started immunoglobulin replacement therapy significantly later than those without this complication [7 (range 2-22) years versus 5 (range 1-13) years]. Patients with a history of LRTI had higher serum IgG concentrations on therapy and were twice as likely to be on prophylactic antibiotics. Ensuring prompt commencement of immunoglobulin therapy in patients with idiopathic hypogammaglobulinaemia is likely to help prevent LRTI and subsequent bronchiectasis. Cancer was the only factor associated with mortality. Overt cancer, both haematological and non-haematological, was associated with significantly lower absolute CD8(+) T cell but not natural killer (NK) cell numbers, raising the question as to what extent immune senescence, particularly of CD8(+) T cells, might contribute to the increased risk of cancers as individuals age.


Subject(s)
Agammaglobulinemia/diagnosis , Bronchiectasis/diagnosis , Common Variable Immunodeficiency/diagnosis , Lung Neoplasms/diagnosis , Registries , Respiratory Tract Infections/diagnosis , Adolescent , Adult , Agammaglobulinemia/drug therapy , Agammaglobulinemia/immunology , Agammaglobulinemia/mortality , Aged , Aged, 80 and over , Bronchiectasis/drug therapy , Bronchiectasis/immunology , Bronchiectasis/mortality , Common Variable Immunodeficiency/drug therapy , Common Variable Immunodeficiency/immunology , Common Variable Immunodeficiency/mortality , Female , Humans , Immunoglobulins/blood , Immunoglobulins, Intravenous/therapeutic use , Leukocyte Count , Lung/drug effects , Lung/immunology , Lung/pathology , Lung Neoplasms/drug therapy , Lung Neoplasms/immunology , Lung Neoplasms/mortality , Male , Middle Aged , Phenotype , Respiratory Tract Infections/drug therapy , Respiratory Tract Infections/immunology , Respiratory Tract Infections/mortality , Risk Factors , Survival Analysis , Time Factors , United Kingdom
6.
J Clin Immunol ; 35(2): 199-205, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25663093

ABSTRACT

INTRODUCTION: Complement immunodeficiencies (excluding hereditary angioedema and mannose binding lectin deficiency) are rare. Published literature consists largely of case reports and small series. We collated data from 18 cities across Europe to provide an overview of primarily homozygous, rather than partial genotypes and their impact and management. METHODS: Patients were recruited through the ESID registry. Clinical and laboratory information was collected onto standardized forms and analyzed using SPSS software. RESULTS: Seventy-seven patients aged 1 to 68 years were identified. 44 % presented in their first decade of life. 29 % had C2 deficiency, defects in 11 other complement factors were found. 50 (65 %) had serious invasive infections. 61 % of Neisseria meningitidis infections occurred in patients with terminal pathway defects, while 74 % of Streptococcus pneumoniae infections occurred in patients with classical pathway defects (p < 0.001). Physicians in the UK were more likely to prescribe antibiotic prophylaxis than colleagues on the Continent for patients with classical pathway defects. After diagnosis, 16 % of patients suffered serious bacterial infections. Age of the patient and use of prophylactic antibiotics were not associated with subsequent infection risk. Inflammatory/autoimmune diseases were not seen in patients with terminal pathway, but in one third of patients classical and alternative pathway defects. CONCLUSION: The clinical phenotypes of specific complement immunodeficiencies vary considerably both in terms of the predominant bacterial pathogen, and the risk and type of auto-inflammatory disease. Appreciation of these phenotypic differences should help both immunologists and other specialists in their diagnosis and management of these rare and complex patients.


Subject(s)
Complement System Proteins/deficiency , Complement System Proteins/genetics , Immunologic Deficiency Syndromes/epidemiology , Immunologic Deficiency Syndromes/genetics , Adolescent , Adult , Aged , Child , Child, Preschool , Complement Activation/genetics , Complement Activation/immunology , Complement System Proteins/immunology , Consanguinity , Databases, Factual , Disease Management , Europe/epidemiology , Female , Genotype , Humans , Immunologic Deficiency Syndromes/diagnosis , Immunologic Deficiency Syndromes/therapy , Infant , Male , Middle Aged , Young Adult
7.
Clin Exp Immunol ; 175(1): 68-78, 2014 Jan.
Article in English | MEDLINE | ID: mdl-23841717

ABSTRACT

This report summarizes the establishment of the first national online registry of primary immune deficency in the United Kingdom, the United Kingdom Primary Immunodeficiency (UKPID Registry). This UKPID Registry is based on the European Society for Immune Deficiency (ESID) registry platform, hosted on servers at the Royal Free site of University College, London. It is accessible to users through the website of the United Kingdom Primary Immunodeficiency Network (www.ukpin.org.uk). Twenty-seven centres in the United Kingdom are actively contributing data, with an additional nine centres completing their ethical and governance approvals to participate. This indicates that 36 of 38 (95%) of recognized centres in the United Kingdom have engaged with this project. To date, 2229 patients have been enrolled, with a notable increasing rate of recruitment in the past 12 months. Data are presented on the range of diagnoses recorded, estimated minimum disease prevalence, geographical distribution of patients across the United Kingdom, age at presentation, diagnostic delay, treatment modalities used and evidence of their monitoring and effectiveness.


Subject(s)
Immunologic Deficiency Syndromes , Internet , Registries , Female , Humans , Immunologic Deficiency Syndromes/diagnosis , Immunologic Deficiency Syndromes/epidemiology , Immunologic Deficiency Syndromes/therapy , Male , United Kingdom/epidemiology
8.
Clin Exp Immunol ; 175(1): 59-67, 2014 Jan.
Article in English | MEDLINE | ID: mdl-23786259

ABSTRACT

Hereditary angioedema (HAE) and acquired angioedema (AAE) are rare life-threatening conditions caused by deficiency of C1 inhibitor (C1INH). Both are characterized by recurrent unpredictable episodes of mucosal swelling involving three main areas: the skin, gastrointestinal tract and larynx. Swelling in the gastrointestinal tract results in abdominal pain and vomiting, while swelling in the larynx may be fatal. There are limited UK data on these patients to help improve practice and understand more clearly the burden of disease. An audit tool was designed, informed by the published UK consensus document and clinical practice, and sent to clinicians involved in the care of HAE patients through a number of national organizations. Data sets on 376 patients were received from 14 centres in England, Scotland and Wales. There were 55 deaths from HAE in 33 families, emphasizing the potentially lethal nature of this disease. These data also show that there is a significant diagnostic delay of on average 10 years for type I HAE, 18 years for type II HAE and 5 years for AAE. For HAE the average annual frequency of swellings per patient affecting the periphery was eight, abdomen 5 and airway 0·5, with wide individual variation. The impact on quality of life was rated as moderate or severe by 37% of adult patients. The audit has helped to define the burden of disease in the UK and has aided planning new treatments for UK patients.


Subject(s)
Angioedemas, Hereditary , Cost of Illness , Medical Audit , Quality of Life , Adult , Angioedemas, Hereditary/diagnosis , Angioedemas, Hereditary/economics , Angioedemas, Hereditary/mortality , Angioedemas, Hereditary/therapy , Female , Humans , Male , Middle Aged , Time Factors , United Kingdom/epidemiology
9.
J Neurooncol ; 108(3): 395-402, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22382786

ABSTRACT

Despite numerous clinical trials over the past 2 decades, the overall survival for children diagnosed with diffuse intrinsic pontine glioma (DIPG) remains 9-10 months. Radiation therapy is the only treatment with proven effect and novel therapies are needed. Epidermal growth factor receptor variant III (EGFRvIII) is the most common variant of the epidermal growth factor receptor and is expressed in many tumor types but is rarely found in normal tissue. A peptide vaccine targeting EGFRvIII is currently undergoing investigation in phase 3 clinical trials for the treatment of newly diagnosed glioblastoma (GBM), the tumor in which this variant receptor was first discovered. In this study, we evaluated EGFRvIII expression in pediatric DIPG samples using immunohistochemistry with a double affinity purified antibody raised against the EGFRvIII peptide. Staining of pediatric DIPG histological samples revealed expression in 4 of 9 cases and the pattern of staining was consistent with what has been seen in EGFRvIII transfected cells as well as GBMs from adult trials. In addition, analysis of tumor samples collected immediately post mortem and of DIPG cells in culture by RT-PCR, western blot analysis, and flow cytometry confirmed EGFRvIII expression. We were therefore able to detect EGFRvIII expression in 6 of 11 DIPG cases. These data suggest that EGFRvIII warrants investigation as a target for these deadly pediatric tumors.


Subject(s)
Brain Stem Neoplasms/genetics , ErbB Receptors/genetics , Adult , Blotting, Western , Brain Stem Neoplasms/metabolism , Child, Preschool , ErbB Receptors/metabolism , Flow Cytometry , Humans , Immunoenzyme Techniques , In Situ Hybridization, Fluorescence , Peptide Fragments/immunology , Prognosis , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction
10.
Genes Chromosomes Cancer ; 34(4): 372-83, 2002 08.
Article in English | MEDLINE | ID: mdl-12112526

ABSTRACT

Activation of the MDR1 (ABCB1) gene is a common event conferring multidrug resistance (MDR) in human cancers. We investigated MDR1 activation in MDR variants of a human sarcoma line, some of which express a mutant MDR1, which facilitated the study of allelic gene expression. Structural alterations of MDR1, gene copy numbers, and allelic expression were analyzed by cytogenetic karyotyping, oligonucleotide hybridization, Southern blotting, polymerase chain reaction, and DNA heteroduplex assays. Both chromosome 7 alterations and several cytogenetic changes involving the 7q21 locus are associated with the development of MDR in these sarcoma cells. Multistep-selected cells and their revertants contain three- to six-fold MDR1 gene amplification compared with that of the drug-sensitive parental cell line MES-SA and single-step doxorubicin-selected mutants. MDR1 gene amplification precedes the emergence of a mutant allele in cells that were coselected with doxorubicin and a cyclosporin inhibitor of P-glycoprotein (P-gp). Allele-specific oligonucleotide hybridization showed that the endogenous mutant allele was present as a single copy, with multiple copies of the normal allele. Reselection of revertant cells with doxorubicin in either the presence or the absence of the P-gp inhibitor resulted in exclusive reexpression of the mutant MDR1 allele, regardless of the presence of multiple wild-type MDR1 alleles. These data provide new insights into how multiple alleles are regulated in the amplicon of drug-resistant cancer cells and indicate that increased expression of an amplified gene can result from selective transcription of a single mutant allele of the gene.


Subject(s)
Alleles , Drug Resistance, Neoplasm/genetics , Gene Amplification/genetics , Gene Expression Regulation, Neoplastic/genetics , Genes, MDR/genetics , Multidrug Resistance-Associated Proteins/genetics , Mutation/genetics , Sarcoma/genetics , ATP Binding Cassette Transporter, Subfamily B, Member 1/biosynthesis , Antineoplastic Agents/pharmacology , Chromosome Painting/methods , Cytogenetic Analysis/methods , DNA Mutational Analysis , DNA, Neoplasm/analysis , DNA, Neoplasm/genetics , Doxorubicin/metabolism , Doxorubicin/pharmacology , Gene Dosage , Genetic Variation/genetics , Humans , Karyotyping , Multidrug Resistance-Associated Proteins/biosynthesis , RNA, Messenger/biosynthesis , Sarcoma/chemistry , Sarcoma/metabolism , Sarcoma/pathology , Tritium/metabolism , Tumor Cells, Cultured
11.
Cancer Genet Cytogenet ; 129(2): 155-60, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11566347

ABSTRACT

The AML1 (CBFA2) gene is the most frequent target of chromosomal rearrangements observed in human acute leukemia. These rearrangements include the commonly reported t(8;21)(q22;q22) or AML1/ETO fusion in AML-M2, the t(3;21)(q26;q22) or AML1 fusion with one of three genes, MDS1, EAP or EVI1, in therapy-related AML and MDS, as well as in blast crisis in CML and the t(12;21)(p13;q22) or TEL/AML1 fusion in B-cell ALL. In addition to the t(3;21), other AML1 translocations have also been reported in therapy-related MDS and AML, particularly after treatment with topoisomerase II inhibitors. AML1 gene rearrangements have also been observed less frequently with numerous other chromosomal partners. Here, we describe a patient with AML-M4 and a previously unreported rearrangement involving the AML1 locus and an unknown locus on the short arm of chromosome 1 at 1p32.


Subject(s)
Chromosomes, Human, Pair 1/genetics , Chromosomes, Human, Pair 21/genetics , DNA-Binding Proteins/genetics , Leukemia, Myelomonocytic, Acute/genetics , Proto-Oncogene Proteins , Transcription Factors/genetics , Translocation, Genetic/genetics , Adult , Bone Marrow Examination , Core Binding Factor Alpha 2 Subunit , Humans , Immunophenotyping , In Situ Hybridization, Fluorescence , Karyotyping , Leukemia, Myelomonocytic, Acute/diagnosis , Male
12.
J Pediatr Hematol Oncol ; 23(7): 452-5, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11878581

ABSTRACT

PURPOSE: The authors report the use of a cyclophosphamide, hydroxydaunorubicin, vincristine, and prednisone (CHOP)-based chemotherapy regimen in treating six children with posttransplantation lymphoproliferative disorder (PTLD) that developed after solid organ transplantation. MATERIALS AND METHODS: The chemotherapy regimen consisted of a 29-day induction with CHOP and then as many as 15 cycles of maintenance therapy using methotrexate and cytarabine alternating with vincristine, adriamycin, mercaptopurine, and prednisone. RESULTS: All patients attained remission. One patient died of sepsis while in remission. Four of the five remaining patients have been followed-up in remission for as long as 8 years without losing the graft. One of the patients experienced relapse after completing therapy and subsequently died with disease. CONCLUSIONS: The authors conclude that pediatric patients with PTLD after solid organ transplantation that fails conservative management can be treated successfully with CHOP-based chemotherapy.


Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Cyclophosphamide/therapeutic use , Doxorubicin/therapeutic use , Lymphoproliferative Disorders/drug therapy , Organ Transplantation/adverse effects , Prednisone/therapeutic use , Vincristine/therapeutic use , Adolescent , Child, Preschool , Humans , Infant , Lymphoproliferative Disorders/etiology , Neoplasm Staging
13.
Appl Immunohistochem Mol Morphol ; 8(3): 222-7, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10981875

ABSTRACT

Spindle cell lipoma demonstrates a distinctive histologic appearance and characteristic clinical presentation. We recently observed two cases of solitary subcutaneous neoplasm of the foot with histologic features of spindle cell lipoma that in one case includes a minor component of the overlapping tumor, pleomorphic lipoma. Because the foot is an unusual location for these neoplasms, immunoperoxidase and cytogenetic studies were performed. In both cases, staining was strongly positive for CD34 and negative for smooth muscle actin. Cytogenetic studies from the tumor with a pleomorphic component revealed features consistent with a lipomatous neoplasm, but are otherwise diagnostically nonspecific. An analysis of the literature reveals that although CD34 immunoreactivity is characteristic of spindle cell lipoma and helps exclude nonlipomatous neoplasms, it does not clearly eliminate other well-differentiated lipomatous tumors. Accordingly, without the aid of classic tumor location, the diagnosis of the spindle cell/pleomorphic lipoma group relies primarily on histologic features, with supportive but not definitive information provided by immunoperoxidase and cytogenetic studies. Obscuring this issue, however, are the imprecise histologic distinction between these tumors and those of the atypical lipoma/atypical lipomatous tumor/ well-differentiated liposarcoma group and the nomenclature controversy that surrounds the latter group of neoplasms. Despite these obstacles, both groups of well-differentiated lipomatous tumors are clinically benign when subcutaneously located.


Subject(s)
Antigens, CD34/biosynthesis , Foot Diseases/metabolism , Foot Diseases/pathology , Lipoma/metabolism , Lipoma/pathology , Neoplasms/metabolism , Neoplasms/pathology , Chromosome Banding , Female , Foot Diseases/genetics , Humans , Immunohistochemistry , Karyotyping , Lipoma/genetics , Male , Middle Aged , Neoplasms/genetics
14.
J Biol Chem ; 274(37): 26141-8, 1999 Sep 10.
Article in English | MEDLINE | ID: mdl-10473565

ABSTRACT

Normal human endothelial cells, like other somatic cells in culture, divide a limited number of times before entering a nondividing state called replicative senescence. Expression of the catalytic component of human telomerase, human telomerase reverse transcriptase (hTERT), extends the life span of human fibroblasts and retinal pigment epithelial cells beyond senescence without causing neoplastic transformation (Bodnar, A. G., Ouellette, M., Frolkis, M., Holt, S. E., Chiu, C. P., Morin, G. B., Harley, C. B., Shay, J. W., Lichtsteiner, S., and Wright, W. E. (1998) Science 279, 349-352; Jiang, X., Jimenez, G., Chang, E., Frolkis, M., Kusler, B., Sage, M., Beeche, M., Bodnar, A., Wahl, G., Tlsty, T., and Chiu, C.-P. (1999) Nat. Genet. 21, 111-114). Here, we show that both human large vessel and microvascular endothelial cells also bypass replicative senescence after introduction of hTERT. For the first time, we report that hTERT expression in these life-extended vascular cells does not affect their differentiated and functional phenotype and that these cells maintain their angiogenic potential in vitro. Furthermore, hTERT(+) microvascular endothelial cells have normal karyotype, and hTERT(+) endothelial cell strains do not exhibit a transformed phenotype. Relative to parental cells at senescence, hTERT-expressing endothelial cells exhibit resistance to induction of apoptosis by a variety of different conditions. Such characteristics are highly desirable for designing vascular transplantation and gene therapy delivery systems in vivo.


Subject(s)
Cellular Senescence , Endothelium, Vascular/cytology , Telomerase/metabolism , Apoptosis/drug effects , Base Sequence , Cell Cycle , Cell Division , Cells, Cultured , Cycloheximide/pharmacology , DNA Primers , Dactinomycin/pharmacology , Endothelium, Vascular/enzymology , Humans , Karyotyping , Lipopolysaccharides/pharmacology , Telomerase/genetics , Tumor Necrosis Factor-alpha/pharmacology
15.
Blood ; 92(9): 3318-27, 1998 Nov 01.
Article in English | MEDLINE | ID: mdl-9787169

ABSTRACT

We have developed culture conditions for the efficient expansion of cytotoxic effector cells from peripheral blood mononuclear cells (PBMNCs) by the timed addition of interferon-gamma (IFN-gamma), interleukin-2 (IL-2), and the monoclonal antibody (MoAb) OKT3. These cells, termed cytokine-induced killer (CIK) cells, are composed primarily of T cells, and the population of cells with the greatest cytotoxic activity is an otherwise rare population of CD3(+)CD56(+) cells that expand dramatically under these culture conditions. CIK cells were expanded from PBMNCs from 13 patients with chronic myeloid leukemia (CML). These cultures contained a variable number of T cells at the start of the culture (median 44%, range 1% to 64%), yet after 21 to 28 days of culture, virtually all of the cells were CD3(+) T cells (median 97%, range 90% to 99%). The CD3(+)CD56(+) subset of cells expanded significantly (median 25-fold, range 2.2- to 525-fold). CIK cells from all patients showed cytotoxicity against the tumor cell lines OCI-LY8 and K562. In four patients the expanded CIK cells suppressed colony growth of autologous CML blast cells and myeloid progenitor cells. Allogeneic CIK cells from normal donors also suppressed CML colony growth but did not inhibit growth of normal hematopoietic colonies. Twelve of the 13 cultures were exclusively composed of Philadelphia (Ph)-negative cells and one culture had 1 out of 20 Ph-positive metaphases after 4 weeks in culture. Intracellular cytokine production was assayed by fluorescence-activated cell sorter (FACS), and the expanded T-cell cultures produced IL-2, IFN-gamma, and tumor necrosis factor-alpha (TNF-alpha), but not IL-4. Both the CD4(+) and CD8(+) subsets secreted this cytokine profile. To test the in vivo activity of the expanded CIK cells, CML was engrafted into severe combined immunodeficiency disease (SCID) mice using matrigel. After 4 weeks, 4 x 10(7) autologous CIK cells were injected intravenously by tail vein injection into groups of mice, and the animals were sacrificed after a total of 18 weeks. Bcr-abl was detected in the bone marrow or spleen of 5 out of 6 control mice and only 2 out of 13 mice who received the autologous CIK cells (P = .02). In an additional series of animals, the mice did not engraft with CML but instead developed large human Epstein-Barr virus-associated lymphomas by 12 weeks. The mice who received autologous CIK cells at 4 weeks had either no tumor (5) or small tumors (5), whereas all 10 mice that received CIK cells at week 8 developed lymphomas; however, these were not as large as in the 10 control mice who did not receive CIK cells (P = . 03). This study shows that CIK cells, which are Ph chromosome-negative, can be expanded from patients with CML and have potent in vitro and in vivo efficacy against autologous tumor cells.


Subject(s)
Graft vs Tumor Effect/immunology , Immunotherapy, Adoptive , Interferon-gamma/pharmacology , Interleukin-2/pharmacology , Killer Cells, Natural/transplantation , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/therapy , Muromonab-CD3/pharmacology , T-Lymphocyte Subsets/immunology , T-Lymphocytes, Cytotoxic/immunology , Animals , CD3 Complex/analysis , CD56 Antigen/analysis , Cell Division/drug effects , Cells, Cultured/transplantation , Collagen , Drug Combinations , Epstein-Barr Virus Infections/transmission , False Negative Reactions , Fusion Proteins, bcr-abl/analysis , Hematopoietic Stem Cells/drug effects , Humans , Immunotherapy, Adoptive/adverse effects , Interferon-gamma/biosynthesis , Interleukin-2/biosynthesis , Karyotyping , Killer Cells, Natural/immunology , Killer Cells, Natural/virology , Laminin , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/immunology , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/pathology , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/virology , Leukemia, Myeloid, Chronic-Phase/pathology , Lymphoma, B-Cell/etiology , Lymphoma, B-Cell/virology , Mice , Mice, SCID , Neoplasm Transplantation , Neoplastic Stem Cells/drug effects , Philadelphia Chromosome , Proteoglycans , T-Lymphocyte Subsets/drug effects , T-Lymphocyte Subsets/virology , T-Lymphocytes, Cytotoxic/drug effects , T-Lymphocytes, Cytotoxic/virology , Transplantation, Heterologous/immunology , Transplantation, Homologous/immunology , Tumor Cells, Cultured , Tumor Necrosis Factor-alpha/biosynthesis , Tumor Stem Cell Assay
16.
Leukemia ; 8(8): 1359-68, 1994 Aug.
Article in English | MEDLINE | ID: mdl-8057674

ABSTRACT

Uncontrolled proliferation of acute myeloid leukemia (AML) cells is an important step during leukemogenesis. However, little is known about the mechanisms leading to growth autonomy. Studies using immortalized murine hematopoietic cell lines have suggested that autocrine production of growth factors, or the constitutive activation of molecules in growth factor signalling pathways, are involved. We have established six spontaneous factor-independent cell lines from the human growth factor-dependent TF-1 cell line. The factor-independent cells showed no detectable growth factor activity. Immunoblotting analyses of tyrosine phosphorylation, Raf-1 and extracellular signal-regulated kinase 2 (ERK-2) showed a similar pattern in all the cell lines including TF-1 cells. Furthermore, somatic-cell hybrids between TF-1 and the factor-independent cells grew in absence of growth factor. Taken together this data demonstrates that the factor independence in this system is dominant and suggests that the molecular event is located either downstream of the Raf-1 and MAP kinases pathway or on an alternative pathway. Finally, the karyotype analysis of one factor-independent cell line TF-1i1 and TF-1H- (G418 resistant, HAT sensitive TF-1 cells) and their hybrids demonstrated an unstable derivative chromosome [der(19) t(19;?) (q13.1;?)] which seemed to correlate with the factor-independence capacity. This model may help in our understanding of autonomous proliferation by human myeloid leukemias.


Subject(s)
Calcium-Calmodulin-Dependent Protein Kinases/biosynthesis , Granulocyte-Macrophage Colony-Stimulating Factor/biosynthesis , Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology , Protein Serine-Threonine Kinases/biosynthesis , Protein-Tyrosine Kinases/biosynthesis , Proto-Oncogene Proteins/biosynthesis , Acute Disease , Animals , Base Sequence , Calcium-Calmodulin-Dependent Protein Kinases/isolation & purification , Cell Division/drug effects , Cell Line , DNA Primers , Granulocyte-Macrophage Colony-Stimulating Factor/genetics , Humans , Leukemia, Myeloid , Mice , Mitogen-Activated Protein Kinase 1 , Molecular Sequence Data , Polymerase Chain Reaction , Protein Serine-Threonine Kinases/isolation & purification , Protein-Tyrosine Kinases/isolation & purification , Proto-Oncogene Proteins/isolation & purification , Proto-Oncogene Proteins c-raf , Tumor Cells, Cultured
17.
Blood ; 82(1): 247-61, 1993 Jul 01.
Article in English | MEDLINE | ID: mdl-8100721

ABSTRACT

Immunosuppressed individuals are at high risk for the development of hematologic malignancies. The typical lymphomas arising in organ transplant recipients are B-cell non-Hodgkin's lymphomas that contain Epstein-Barr virus (EBV) DNA sequences. We investigated the characteristics of posttransplant lymphomas that lacked expression of the usual markers associated with EBV transformation. We describe four large-cell lymphomas seen recently at our institution. Two of these four cases were CD4+, one was CD8+, and in one staining for CD4 and CD8 expression was not performed. One CD4+ lymphoma was a CD30+, EBV- large-cell lymphoma from a 65-year-old kidney transplant recipient, the second was an EBV+ large-cell lymphoma from a 25-year-old heart transplant patient. Two T-cell lymphomas were EBV+ and had clonal T-cell receptor beta gene rearrangements. The other two lymphomas expressed T-cell markers CD4 and CD43, and lacked expression of B-cell markers CD19, CD20, CD21, CD22, CD23, and surface Ig. Both CD4+ lymphomas were tumorigenic after their heterotransplantation into severe combined immunodeficient (SCID) mice. Cytogenetics, immunophenotyping, and genotyping of the secondary tumors from SCID mice showed their clonality and identity with the patients' primary tumors. Novel CD4+ lymphoma cell lines, LH521/4 and LK418/4, were established from tumors that had been passaged in SCID mice. An immunodeficient environment may facilitate the growth of these T-cell or biphenotypic lymphomas; the etiology of their genesis can include transformation with EBV and other, as yet unidentified mechanisms.


Subject(s)
Antigens, Differentiation, T-Lymphocyte/analysis , Immunosuppression Therapy/adverse effects , Lymphoma/pathology , Transplantation/adverse effects , Adolescent , Animals , CD4-Positive T-Lymphocytes/pathology , Child, Preschool , Chromosome Aberrations/pathology , Chromosome Disorders , DNA, Viral/analysis , Gene Rearrangement, B-Lymphocyte , Gene Rearrangement, beta-Chain T-Cell Antigen Receptor , Genes, Immunoglobulin , Herpesvirus 4, Human/genetics , Humans , Immunophenotyping , Karyotyping , Lymphoma/genetics , Lymphoma/immunology , Male , Mice , Mice, SCID , Middle Aged , Neoplasm Transplantation , Pleural Effusion/pathology , Tumor Virus Infections/complications
18.
Blood ; 78(6): 1552-60, 1991 Sep 15.
Article in English | MEDLINE | ID: mdl-1884022

ABSTRACT

The t(14;18) chromosomal translocation that results in the juxtaposition of the bcl-2 proto-oncogene with the heavy chain JH locus is a common cytogenetic abnormality in human lymphoma. In particular, it is seen in about 85% of follicular lymphoma (FL) and up to one-third of diffuse lymphomas (DL). The chromosome 18 breakpoints have been shown to cluster into two regions. The major breakpoint region (mbr) within the 3' untranslated region of the bcl-2 proto-oncogene accounts for approximately 60% of the cases and the minor cluster region (mcr) 30 kb 3' of bcl-2 accounts for approximately 25% of the breakpoints. Because of variability in the position of the breakpoint, detection of the t(14;18) by Southern blot analysis provides an important clonal marker for the tumor. However, conventional electrophoresis (CE) fails to detect the translocation in 15% to 25% of cases. We have applied pulsed-field gel electrophoresis (PFGE) to the detection of the t(14;18) in a series of lymphoma prospectively analyzed by CE, polymerase chain reaction (PCR), and cytogenetic analysis. PFGE readily detected t(14;18) rearrangements as indicated by comigration of bands detected with probes for the mbr region (chromosome 18) and the JH locus (chromosome 14). In a series of 40 patients with FL, this method proved to be the most comprehensive for detection of the translocation compared with standard methods; in fact, in one case only PFGE was able to detect the chromosomal rearrangement. Ten percent of the FL cases were negative by all methods tested. In a separate analysis of matched tissue specimens from cases of tumor progression of FL to diffuse lymphoma, PFGE detected a common t(14;18) rearrangement confirming a clonal origin in seven of seven cases, whereas CE detected a rearrangement in only three of seven cases. Overall, PFGE was able to detect a translocation in 8 of 12 cases that were negative by CE and four of eight negative by cytogenetic analysis. In conclusion, PFGE analysis is more comprehensive than CE, PCR, and cytogenetic analysis for the detection of the t(14;18) breakpoint in tissue biopsies of malignant lymphoma.


Subject(s)
Chromosomes, Human, Pair 14 , Chromosomes, Human, Pair 18 , DNA, Neoplasm/analysis , Electrophoresis, Agar Gel/methods , Translocation, Genetic/genetics , Chromosome Mapping , Humans , Karyotyping , Nucleic Acid Hybridization , Polymerase Chain Reaction , Prospective Studies , Proto-Oncogene Mas
19.
Blood ; 76(7): 1299-307, 1990 Oct 01.
Article in English | MEDLINE | ID: mdl-1698477

ABSTRACT

Marrow cells from 36 patients with myelodysplastic syndromes (MDS) (13 refractory anemia [RA], 14 refractory anemia with excess of blasts [RAEB], 9 RAEB in transformation [RAEB-T]) were evaluated for their in vitro proliferative and differentiative responsiveness to recombinant human granulocyte colony-stimulating factor (G-CSF) or granulocyte-monocyte CSF (GM-CSF). GM-CSF exerted a stronger proliferative stimulus than G-CSF for marrow myeloid clonal growth (CFU-GM) in these patients (44 v 12 colonies per 10(5) nonadherent buoyant bone marrow cells [NAB], respectively, P less than .025). GM-CSF stimulated increased CFU-GM growth in the 16 patients with abnormal marrow cytogenetics in comparison with the 20 patients who had normal cytogenetics (52 and 30 colonies per 10(5) NAB, respectively, P less than .05), whereas no such difference could be demonstrated with G-CSF (11 and 16 colonies per 10(5) NAB, respectively). In contrast, granulocytic differentiation of marrow cells was induced in liquid culture by G-CSF in 15 of 32 (47% patients), while GM-CSF did so in only 4 of 18 (22%) patients (P less than .025) including, for RAEB/RAEB-T patients: 9 of 18 versus 0 of 9, respectively (P less than .025). For MDS patients with normal cytogenetics, G-CSF- and GM-CSF-induced marrow cell granulocytic differentiation in 12 of 18 (67%) versus 3 of 11 (27%), respectively (P less than .025), contrasted with granulocytic induction in only 3 of 14 (21%) and 1 of 7 (14%) patients with abnormal cytogenetics, respectively. We conclude that G-CSF has greater granulocytic differentiative and less proliferative activity for MDS marrow cells than GM-CSF in vitro, particularly for RAEB/RAEB-T patients and those with normal cytogenetics.


Subject(s)
Bone Marrow/pathology , Hematopoiesis/drug effects , Myelodysplastic Syndromes/pathology , Aged , Aged, 80 and over , Bone Marrow/drug effects , Cell Differentiation/drug effects , Cell Division/drug effects , Cells, Cultured , Evaluation Studies as Topic , Female , Granulocyte Colony-Stimulating Factor/pharmacology , Granulocyte Colony-Stimulating Factor/therapeutic use , Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology , Granulocyte-Macrophage Colony-Stimulating Factor/therapeutic use , Humans , Karyotyping , Male , Middle Aged , Myelodysplastic Syndromes/drug therapy , Myelodysplastic Syndromes/genetics , Recombinant Proteins/pharmacology , Recombinant Proteins/therapeutic use
20.
Cancer ; 66(3): 474-9, 1990 Aug 01.
Article in English | MEDLINE | ID: mdl-2364360

ABSTRACT

The authors performed immunohistochemical and cytogenetic studies in a 73-year old man with malignant angioendotheliomatosis. The patient was referred for evaluation of fever of unknown origin, hepatic failure, and neurologic deterioration. Examination of a muscle biopsy revealed numerous, noncohesive atypical mononuclear cells within small vessels. These cells stained positively with a pan-leukocyte marker CD45(PD7/26/16) and with a B-cell marker L26 but negatively with Factor VIII-related antigen, an endothelial cell marker. Peripheral blood obtained before chemotherapy was cultured and analyzed by the G-band method. A new translocation and numerous chromosomal aberrations were identified. The major cell line karyotype was 53,XY, +X, +5q?,-6, +i(6p), +7, -10, +11, -12, +12p-, +12p-, +18, +mar1, +mar2, t(1;3)(p22;p21),3q+,8p+. This is the first cytogenetic study performed in a case of malignant angioendotheliomatosis. Our findings demonstrate that the neoplastic cells in this disorder circulate in the peripheral blood and provide further evidence that malignant angioendotheliomatosis is a diffuse intravascular neoplasm of lymphoid origin. Furthermore, the authors conclude that this malignant lymphoproliferative disorder should be reclassified as a primary intravascular (angiotropic) lymphoma.


Subject(s)
Hemangioendothelioma/classification , Lymphoma/classification , Skin Neoplasms/classification , Vascular Diseases/classification , Aged , Fever of Unknown Origin/etiology , Hemangioendothelioma/complications , Hemangioendothelioma/genetics , Hemangioendothelioma/pathology , Humans , Immunohistochemistry , Karyotyping , Lymphoma/complications , Lymphoma/genetics , Lymphoma/pathology , Male , Skin Neoplasms/complications , Skin Neoplasms/genetics , Skin Neoplasms/pathology , Vascular Diseases/complications , Vascular Diseases/genetics , Vascular Diseases/pathology
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