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J Magn Reson ; 191(2): 202-18, 2008 Apr.
Article in English | MEDLINE | ID: mdl-18280189

ABSTRACT

Inter-spin distances between 1 nm and 4.5 nm are measured by continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) methods for a series of nitroxide-spin-labelled peptides. The upper distance limit for measuring dipolar coupling by the broadening of the CW spectrum and the lower distance limit for the present optimally-adjusted double electron electron resonance (DEER) set-up are determined and found to be both around 1.6-1.9 nm. The methods for determining distances and corresponding distributions from CW spectral line broadening are reviewed and further developed. Also, the work shows that a correction factor is required for the analysis of inter-spin distances below approximately 2 nm for DEER measurements and this is calculated using the density matrix formalism.


Subject(s)
Algorithms , Electron Spin Resonance Spectroscopy/methods , Models, Chemical , Peptides/chemistry , Computer Simulation , Spin Labels
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