ABSTRACT
Multi-unit activity was recorded in awake cats from the crossed olivocochlear bundle (COCB). Miniature stainless stell concentric electrodes were chronically implanted onto the floor of the fourth ventricle of six animals. There was no activity at the electrode tip in anesthetized animals, while in awake cats a great deal of unit activity could be seen. A correlation between COCB activity and ongoing behavioral activity such as scratching, grooming, yawning, or orientation could not be established. It was found, however, that the multi-unit responses in the COCB statistically increased their firing rate during acoustic stimulation, and a 500-Hz tone was found to be most effective. The electrode locations were histologically confirmed. The present results are similar to other data that describe unit activity in the olivocochlear bundle of decerebrate cats. The capacity to record from this fiber tract in awake animals, however, provides a new tool for studying the peripheral efferent pathway of the auditory system.
Subject(s)
Cochlear Nerve/physiology , Olivary Nucleus/physiology , Acoustic Stimulation , Animals , Behavior, Animal , Cats , Electrodes, Implanted , Evoked Potentials , Motor Activity , Neurons, Efferent/physiologyABSTRACT
A sensitive and specific radioimmunoassay of dog and human plasma pentazocine is described. Rabbit antiserum and the second antibody method separated bound from free pentazocine. The radioimmunoassay employed an 125I-labeled radioligand and required extraction from the sample prior to quantitation. The method had a detection limit of approximately 200 pg/assay tube (1 ng/ml). The assay was used successfully to measure pentazocine in the plasma of beagle hounds given 0.3 mg of pentazocine/kg iv. The decline in plasma levels fitted a two-compartment body model with a 100-min mean overall half-life and a 3.2-liters/hr mean plasma clearance rate.
Subject(s)
Pentazocine/blood , Animals , Antibody Specificity , Dogs , Evaluation Studies as Topic , Female , Humans , Male , Methods , Models, Biological , Pentazocine/immunology , RadioimmunoassayABSTRACT
A sensitive method is described for the radioimmunoassay of danazol in monkey and human plasma. Antiserum was developed in rabbits, and a second antibody was used to separate bound from free danazol. The radioimmunoassay was specific for danazol, and the limit of detection ranged from 1.4 to 2.8 ng/ml. Exogeneous danazol could be quantitated accurately in both monkey and human plasma. The radioimmunoassay results agreed with values obtained by inverse isotope dilution after intravenous administration of 14C-danazol to monkeys. The assay was used successfully to measure danazol in plasma from human volunteers receiving 200 mg of danazol.
