ABSTRACT
A comparative experimental study of biomicroscopic appearance of lenses in cataracts of different genesis (age-related, ultraviolet and other radiation-induced or combined) has been performed on animals (mice). It is shown that identical lens opacification can be provoked by aging (endogenous factor), as well as ultraviolet and other radiation exposure (exogenous physical factors). The only differential sign is the severity of the damage. These factors can be arranged in the following ascending order by their damaging ability: aging --> ultraviolet --> gamma rays --> gamma rays + ultraviolet. Anti-cataract effect of a chaperone-like combined drug (N-acetylcarnosin and D-pantetin) has been studied in vivo on a "prolonged" model of induced cataract in rats. The use of the combined drug (1:1 mixture of the two peptides) in the form of ocular instillations and intraperitoneal injections helped slowing the progression of the ultraviolet-induced cataract in vivo.
Subject(s)
Cataract/etiology , Gamma Rays/adverse effects , Lens, Crystalline , Molecular Chaperones/toxicity , Ultraviolet Rays/adverse effects , Animals , Cataract/pathology , Disease Models, Animal , Disease Progression , Dose-Response Relationship, Drug , Dose-Response Relationship, Radiation , Lens, Crystalline/drug effects , Lens, Crystalline/pathology , Lens, Crystalline/radiation effects , Male , Mice , Mice, Inbred C57BL , Microscopy, Acoustic , Risk FactorsABSTRACT
Microscopic lens changes are studied in mice (F1C57B1XCBA) depending on age and after ultraviolet (UV), gamma-irradiation and their combination. In all animals compared to young 3-months animals nonspecific changes due to aging were revealed: microvacuoles in cytoplasm of fiber cells, flattening of epithelial cells and nuclei fragmentation, swelling and confluence of cortical layer cells in anterior and posterior subcapsular zones. In epithelial cells the following changes were noted: nuclei polymorphism, cavities in a cell layer formation of multilayered structures of fibroblast-like cells and cell desquamation. Degree of nuclei vacuolization was significantly lower in groups with gamma- and gamma+UV-irradiation but higher in a group of UV exposure. The number of cells per unit area was significantly decreased in a group of gamma-irradiation after 7 and 10 months of exposure. Aging and UV exposure caused decrease of nuclei area, whereas affect of gamma-irradiation depended on proliferative capacity of cells: size of central cells increased and of peripheral cells reduced. Specific changes were not revealed for any of the factors.
Subject(s)
Aging , Cataract , Gamma Rays/adverse effects , Lens, Crystalline , Ultraviolet Rays/adverse effects , Aging/pathology , Animals , Cataract/etiology , Cataract/pathology , Disease Models, Animal , Dose-Response Relationship, Radiation , Lens, Crystalline/pathology , Lens, Crystalline/radiation effects , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Microscopy/methods , Severity of Illness IndexABSTRACT
Comparative study of lens biomicroscopy in cataract of different etiology (senile, ultraviolet, radioactive and combined ultraviolet- radioactive exposure) is performed in experiment on animals (mice). Lens opacification pattern was showed to be similar in aging as an endogenous factor and ultraviolet (UV) and radioactive exposure as exogenous physical factors. Specificity of these factors is expressed in degree of damage only. Depending on damaging potential the factors may be arranged in a following way (in increasing manner): aging, ultraviolet irradiation, gamma-irradiation, gamma+UV-irradiation.
Subject(s)
Aging/physiology , Cataract , Gamma Rays/adverse effects , Lens, Crystalline , Ultraviolet Rays/adverse effects , Animals , Cataract/diagnosis , Cataract/etiology , Cataract/physiopathology , Diagnostic Techniques, Ophthalmological , Disease Models, Animal , Dose-Response Relationship, Radiation , Humans , Lens, Crystalline/physiopathology , Lens, Crystalline/radiation effects , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Microscopy/methods , Risk Factors , Severity of Illness IndexABSTRACT
Using differential electrophoresis protein composition of lens major proteins in hybrid mice F1 (C57B1XCBA) with cataracts of different etiology (senile, ultraviolet, radioactive and combined ultraviolet-radioactive exposure) was studied Changes that may be specific for cataract caused by aging, ultraviolet and/or gamma-irradiation were not revealed in water-soluble and water-insoluble protein fractions.
Subject(s)
Aging/metabolism , Cataract , Crystallins , Gamma Rays/adverse effects , Lens, Crystalline , Solubility/radiation effects , Ultraviolet Rays/adverse effects , Animals , Cataract/etiology , Cataract/metabolism , Crystallins/metabolism , Crystallins/radiation effects , Disease Models, Animal , Dose-Response Relationship, Radiation , Electrophoresis/methods , Lens, Crystalline/metabolism , Lens, Crystalline/radiation effects , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Severity of Illness IndexABSTRACT
Four randomized groups of male mice F1 (C57Black/CBA) were investigated: a) UV-irraidated (UV-A, 15 min daily during 9 months, dose 5.6 + 1.2 W/m2), b) gamma-rays irradiated (2 Gy), single, c) influence for combination of UV- and gamma-rays treatment, d) aging. The lens opacities were measured on 7th and 10th month. The expert method based on six grade scale was used for cataract measure. On 7th month the median of lens opacities were Aging group = 0; UV-irradiated group = 2.5; gamma-irradiated group = 4.75; gamma- and UV-irradiated group = 6.0. The difference between all groups was significant (p < 0.004, Kruskall-Wallis ANOVA test). The Conover post hock test has shown the significant difference for all comparison pairs (p < 0.002) with the exception of UV-irradiated group, gamma-irradiated group, gamma- and UV-irradiated group. On 10th month the lens opacities were strongly increased: Aging group = 2.5; UV-irradiated group = 5.0; gamma-irradiated group = 6.5; gamma- and UV-irradiated group = 7.5 (median). The difference between groups was significant (p < 0.0001, Kruskall-Wallis ANOVA test). The Conover post hock test has shown the significant difference for all comparison pairs (p <0.003) with the exception ofthe UV-irradiated group and gamma-irradiated group. The formation of the specific lens opacities for any group was not found. Morphology and protein composition have been investigated on 10th month. The results of morphological changes study show destructive and degenerative impairments of capsule, epithelium cells and lens fibers. However, no specific changes related to some particular actions have been found. In addition, there were no specific changes of protein composition of both water-soluble and water-insoluble fractions estimated with Differential Gel Electrophoresis technique. Obtained data mean that aging, UV-treatment, and gamma-radiation causes the similar changes of lens. It was supposed that UV-treatment or/and gamma-radiation act as an aging factor on the lens.
