Seed nano-priming with multiple nanoparticles enhanced the growth parameters of lettuce and mitigated cadmium (Cd) bio-toxicity: An advanced technique for remediation of Cd contaminated environments.

Seed nano-priming can be used as an advanced technology for enhancing seed germination, plant growth, and crop productivity; however, the potential role of seed nano-priming in ameliorative cadmium (Cd) bio-toxicity under Cd stress has not yet been sufficiently investigated. Therefore, in this study we investigated the beneficial impacts of seed priming with low (L) and high (H) concentrations of nanoparticles including nSiO2 (50/100 mg L-1), nTiO2 (20/60 mg L-1), nZnO (50/100 mg L-1), nFe3O4 (100/200 mg L-1), nCuO (50/100 mg L-1), and nCeO2 (50/100 mg L-1) on lettuce growth and antioxidant enzyme activities aiming to assess their efficacy for enhancing plant growth and reducing Cd phytotoxicity. The results showed a significant increase in plant growth, biomass production, antioxidant enzyme activities, and photosynthetic efficiency in lettuce treated with nano-primed nSiH + Cd (100 mg L-1), nTiH + Cd (60 mg L-1), and nZnL + Cd (50 mg L-1) under Cd stress. Moreover, nano-priming effectively reduced the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) in lettuce shoots. Interestingly, nano-primed nSiH + Cd, nTiH + Cd, and nZnL + Cd demonstrated efficient reduction of Cd uptake, less translocation factor of Cd with high tolerance index, ultimately reducing toxicity by stabilizing the root morphology and superior accumulation of critical nutrients (K, Mg, Ca, Fe, and Zn). Thus, this study provides the first evidence of alleviating Cd toxicity in lettuce by using multiple nanoparticles via priming strategy. The findings highlight the potential of nanoparticles (Si, Zn, and Ti) as stress mitigation agents for improved crop growth and yield in Cd contaminated areas, thereby offering a promising and advanced approach for remediation of Cd contaminated environments.

Assessing the effectiveness of 3, 4-dimethylpyrazole phosphate (DMPP) inhibitor in mitigating N2O emissions from contrasting Cd-contaminated soils.

Improving nitrogen use efficiency of chemical fertilizers is essential to mitigate the negative environmental impacts of nitrogen. Nitrification, the conversion of ammonium to nitrate via nitrite by soil microbes, is a prominent source of nitrogen loss in soil systems. The effectiveness of nitrification inhibitors in reducing nitrogen loss through inhibition of nitrification is well-documented, however, their efficacy in heavy metals-contaminated soils needs thorough investigations. The current study assessed the efficacy of nitrification inhibitor 3, 4-dimethylpyrazole phosphate (DMPP) in reducing nitrous oxide (N2O) emissions in cadmium (Cd) contaminated paddy and red soils under lab-controlled environment. Obtained results indicated the substantial reduction in N2O emissions with DMPP in paddy and red soil by 48 and 35 %, respectively. However, Cd contamination resulted in reduced efficacy of DMPP, thus decreased the N2O emissions by 36 and 25 % in paddy and red soil, respectively. It was found that addition of DMPP had a significant effect on the abundance of ammonia oxidizing bacteria (AOB) and archaea (AOA). Notably, the reduction in N2O emissions by DMPP varied with the abundance of AOB. Moreover, Cd pollution resulted in a significant (P < 0.05) reduction in the abundance of archaeal and bacterial amoA genes, as well as bacterial nirK, nirS, and nosZ genes. The combined treatment of Cd and DMPP had a detrimental impact on denitrifiers, thereby influencing the overall efficiency of DMPP. These findings provide novel insights into the application of DMPP to mitigate nitrification and its potential role in reducing N2O emissions in contaminated soils.

Effect of foliar application of nanoparticles on growth, physiology, and antioxidant enzyme activities of lettuce (Lactuca sativa L.) plants under cadmium toxicity.

Nanotechnology has attracted the interest of scientists due to its wide range of application specifically in agriculture. Nanoparticles (NPs) may act as a promising materials to alleviate cadmium (Cd) stress in plants. This study aims to assess the impact of multiple nanoparticles including nSiO2 (50 mg L-1:100 mg L-1), nTiO2 (20 mg L-1:60 mg L-1), nZnO (50 mg L-1:100 mg L-1), nFe3O4 (100 mg L-1:200 mg L-1), nCuO (50 mg L-1:100 mg L-1), and nCeO2 (50 mg L-1:100 mg L-1) in combination with CdCl2 (5 µM) to mitigate Cd toxicity in lettuce through foliar application in hydroponic solution. Current findings indicate that foliar application of nSiL + Cd (50 mg L-1), nZnL + Cd (50 mg L-1), and nTiL + Cd (20 mg L-1) is more effective in improving growth, biomass, root architecture, and elevated photosynthetic efficiency, which might be attributed to the increasing uptake of essential micronutrient (K, Mg, Ca, Fe, Zn) under Cd stress. Similarly, treatment with nanoparticles leads to reduced accumulation of ROS and MDA in lettuce, while enhancing the SOD, POD, CAT, and APX activities. The results showed that nanoparticles have high tolerance against Cd as depicted by the inhibition in Cd accumulation by 3.2-58% and 10-72% in roots as well as edible parts of lettuce, respectively. In addition, Cd alone reduces the morphological traits, antioxidant enzyme activity, and photosynthetic activity, while increasing the ROS, MDA, and Cd accumulation in lettuce. This comprehensive study suggests the role of nanoparticles in reducing Cd toxicity in lettuce, signifying their importance as stress mitigation agents. However, long-term pot, priming, and field trials are needed to identify the optimal nanoparticle for the lettuce under variable environmental conditions.