ABSTRACT
The aim of this study was to evaluate and compare the effects of 5-lipoxygenase enzyme (5-LO) inhibitor zileuton and cysteinyl leukotriene receptor (CysLT1R) antagonist montelukast in testicular torsion/detorsion (T/D) injury model in rats. Rats were anaesthetised with 75 mg kg(-1) ketamine hydrochloride and 8 mg kg(-1) xylazine intraperitoneal before the operation. Torsion was created by rotating the right testis 720° clockwise and maintained by fixing the testis. The rats were treated with CysLT1R antagonist montelukast (10 mg kg(-1); i.p.), 5-LO inhibitor zileuton (3 mg kg(-1); i.p.), and vehicle, at 30 min prior detorsion. After 1 h of torsion, the testis was counter-rotated to the natural position and replaced into the scrotum. Malondialdehyde (MDA) level was measured in testicular tissue after 3 h of reperfusion. Histological examination was performed after 24 h of reperfusion. T/D caused a significant increase in MDA level and histopathological injury in testes. Montelukast and zileuton treatments prevented the T/D-induced augmentation in MDA levels. Only zileuton treatment significantly reduced the T/D-induced histopathological injury. In this study, we demonstrated for the first time that zileuton had protective effects on testicular T/D injury. We have also found that zileuton is more effective than montelukast on histopathological injury.
Subject(s)
Acetates/therapeutic use , Hydroxyurea/analogs & derivatives , Quinolines/therapeutic use , Spermatic Cord Torsion/drug therapy , Animals , Cyclopropanes , Hydroxyurea/therapeutic use , Male , Malondialdehyde/metabolism , Rats , Rats, Wistar , Spermatic Cord Torsion/metabolism , SulfidesABSTRACT
The antiulcerogenic effect of a hexane extract (HRe-1) from Hippophae rhamnoides (Eleagnaceae) was tested on indomethacin- and stress-induced ulcer models. As a result HRe-1 was found to be active in preventing gastric injury.
Subject(s)
Anti-Ulcer Agents/therapeutic use , Phytotherapy , Plant Extracts/therapeutic use , Rosales , Stomach Ulcer/drug therapy , Animals , Disease Models, Animal , Famotidine/therapeutic use , Fruit , Indomethacin , Male , Misoprostol/therapeutic use , Omeprazole/therapeutic use , Rats , Rats, Wistar , Stomach Ulcer/chemically induced , Stress, PhysiologicalABSTRACT
In this study, the effect of an aqueous extract of Rumex patientia L. (Polygonaceae) (D-1) on capillary permeability which was induced by xylol and hyaluronidase was investigated. Experiments were conducted on rabbits according to Monakova and Matusis methods. The effects of D-1 were compared to those of indomethacin, which was used as a control throughout the experiment. Both D-1 (100 mg/kg) and indomethacin (10 mg/kg) were administered orally. As a result, D-1 inhibited capillary permeability, which was induced by xylol and hyaluronidase, and it was found that it was as effective as indomethacin.
Subject(s)
Capillary Permeability/drug effects , Hyaluronoglucosaminidase/pharmacology , Plants, Medicinal/chemistry , Polygonaceae/chemistry , Xylenes/pharmacology , Animals , Plant Extracts/pharmacology , RabbitsABSTRACT
Pharmacological studies were conducted with the aqueous extract of roots of Rumex patientia L. (Polygonaceae) on experimental animals. For evaluating the antiinflammatory activity, carrageenan, histamine, dextrane, serotonine formaldehyde-induced oedema tests, cotton-pellet granuloma, and Kabak tests in rats were used. The extract was found to possess antiinflammatory activity. Acute toxicity studies revealed that the extract up to a dose of 3 mg/kg orally was nontoxic.
