C-reactive protein (CRP) and Erythrocyte Sedimentation Rate (ESR) Trends following Total Hip and Knee Arthroplasties in an Indian Population - A Prospective Study.

INTRODUCTION: To evaluate the trends of C-reactive protein (CRP) and Erythrocyte sedimentation rate (ESR) in the first three weeks after uncomplicated total hip (THR) and total knee (TKR) arthroplasty/replacement in the Indian population and to compare it with available literature. MATERIALS AND METHODS: A total of 90 patients were enrolled for this prospective study, of which 30 were unilateral THR, 30 were unilateral TKR (U/L TKR) and 30 were simultaneous bilateral TKR (B/L TKR). Serum CRP and ESR were measured on the day before surgery and post-operatively on day 1st, 2nd, 3rd, 7th, 12th, and at the end of 3rd week. RESULTS: CRP showed a peak at day 2nd with normalisation to pre-operative value by the end of 3rd week. While ESR showed a peak on day 3rd and continued to remain elevated even at end of 3rd week post-operatively. Both mean CRP and ESR values were higher in THR patients followed by in B/L TKR and then in U/L TKR patients. CONCLUSION: CRP persists to be the best acute phase reactant in the early post-operative phase with a relatively typical pattern as compared to ESR. CRP values peak at post-operative day 2nd and then show a gradual decline. However, its normalisation to pre-operative baseline values may vary among different groups of population.

C-reactive protein (CRP) and Erythrocyte Sedimentation Rate (ESR) Trends following Total Hip and Knee Arthroplasties in an Indian Population – A Prospective Study

@#Introduction: To evaluate the trends of C-reactive protein (CRP) and Erythrocyte sedimentation rate (ESR) in the first three weeks after uncomplicated total hip (THR) and total knee (TKR) arthroplasty/replacement in the Indian population and to compare it with available literature. Material and methods: A total of 90 patients were enrolled for this prospective study, of which 30 were unilateral THR, 30 were unilateral TKR (U/L TKR) and 30 were simultaneous bilateral TKR (B/L TKR). Serum CRP and ESR were measured on the day before surgery and postoperatively on day 1st, 2nd, 3rd, 7th, 12th, and at the end of 3rd week. Results: CRP showed a peak at day 2nd with normalisation to pre-operative value by the end of 3rd week. While ESR showed a peak on day 3rd and continued to remain elevated even at end of 3rd week post-operatively. Both mean CRP and ESR values were higher in THR patients followed by in B/L TKR and then in U/L TKR patients. Conclusion: CRP persists to be the best acute phase reactant in the early post-operative phase with a relatively typical pattern as compared to ESR. CRP values peak at postoperative day 2nd and then show a gradual decline. However, its normalisation to pre-operative baseline values may vary among different groups of population.

Effects of the intramyocardial implantation of stromal vascular fraction in patients with chronic ischemic cardiomyopathy.

BACKGROUND: Stromal vascular fraction (SVF) can easily be obtained from a mini-lipoaspirate procedure of fat tissue. The SVF contains a mixture of cells including ADSCs and growth factors and has been depleted of the adipocyte (fat cell) population. We evaluated the safety and efficacy of administering SVF intra-myocardially into patients with chronic ischemic cardiomyopathy. METHODS: A total of 28 patients underwent a local tumescent liposuction procedure to remove approximately 60 ml of fat tissue. The fat was separated to isolate the SVF and the cells were delivered into the akinetic myocardial scar region using a transendocardial delivery system (MyoCath(®)) in patients who had experienced a previous myocardial infarct. The subjects were then monitored for adverse events, ejection fraction via echocardiogram and six-minute walk test (6MWT) over a period of 6 months. RESULTS: The average EF was 29 % at baseline and significantly increased to 35 % at both 3 and 6 months. Patients walked an average of 349 m at baseline and demonstrated a statistically significant improvement at 3 and 6 months' post treatment of more than 80 m. CONCLUSIONS: Overall, patients were pleased with the treatment results. More importantly, the procedure demonstrated a strong safety profile with no severe adverse events or complications linked to the therapy. Trial registration NCT01502514 Name of registry: http://www.clinicaltrials.gov URL: https://www.clinicaltrials.gov/ct2/show/NCT01502514?term=adipose+cells+heart&rank=4 Date of registration: December 27, 2011 Date of enrollment: January 2012.

Modulation of macrophage structure and function by low level He-Ne laser irradiation.

Studies have shown that He-Ne laser irradiation can affect the biological functions of macrophages. The aim of the present study was to investigate the effect of He-Ne laser irradiation on the various functional parameters of macrophages and look for possible correlations in the effects to understand the mechanisms involved. Mice peritoneal macrophages were irradiated with a He-Ne laser (632.8 nm, approximately 10 W m(-2)) at energy densities ranging from 100 to 600 J m(-2) and the activities of lysozyme and cathepsin, phagocytosis, and cell spreading (markers of cell activation), as well as changes in NAD(P)H autofluorescence, were monitored. He-Ne laser irradiation was observed to lead to significant changes in all the parameters investigated. While lysozyme activity and spreading of the peripheral membrane were found to increase with the irradiation dose over the dose range investigated, the phagocytotic activity of macrophages, the activity of cathepsin, the observed decease in cell membrane fluidity and the observed increase in NAD(P)H level showed a peak at 200 J m(-2). Possible reasons for and the significance of the observed correlations are discussed.

A solid liquid extraction and preconcentration method for the atomic absorption spectrometric determination of trace cobalt in various alloys and biological samples.

Cobalt is quantitatively retained as cobalt-2-nitroso-4-sulfonic acid (nitros-S)-tetradecyldimethylbenzylammonium (TDBA+) ion-pair complex on microcrystalline naphthalene in the pH range 6.2-9.0 from a large volume of its aqueous solution of various complex samples. After filtration, the solid mass consisting of cobalt complex and naphthalene is dissolved in 5 ml of dimethylformamide (DMF) and the metal is determined by flame atomic absorption spectrometry (FAAS). Cobalt complex can alternatively be quantitatively adsorbed on tetradecyldimethylbenzylammonium chloride-naphthalene adsorbent packed in a column and determined similarly. The calibration curve is linear over the concentration range 0.2-11.5 micrograms of cobalt in 5 ml of the final DMF solution. In this case, 0.2 microgram of cobalt may be concentrated in a column from 250 ml of aqueous sample where its concentration is as low as 0.8 ng/ml. Eight replicate determinations of 3.0 micrograms of cobalt in the final DMF solution give a mean absorbance of 0.045 with a relative standard deviation of 1.8%. The sensitivity for 1% absorption is 59 ng/ml. The interference of a large number of anions and cations on the determination of cobalt has been studied and the optimum conditions developed utilized for its trace determination in various alloys and biological samples. The method may also be used for the determination of cobalt in some environmental samples.

Hybridization and selection for improving seed protein in barley.

Hybridization followed by continuous selection of lines of barley from different cross-combinations involving high protein-high lysine genotypes and the agronomically superior strains resulted in breaking the negative correlations between 1000 grain weight and high protein content and high DBC values. The methodology of DBC-Kjeldahl protein adopted in the present study is likely to be useful in identifying high lysine lines. The present study has shown considerable variability with respect to protein content and grain weight and has provided interesting genotypes which can be used in synthesising lines with improved nutritional quality and productivity in barley. The success in breaking the undesirable linkages to factors that impair the endosperm development is due to sufficient genetic variability in the initial breeding material as well as the use of suitable breeding procedures like the full-sib mating in the early segregating generations.

Effect of autopolyploidy on quantity and quality of protein in barley.

The range and mean protein content of autotetraploids of high-lysine Notch-2 mutants of barley were consistently higher than the diploids in C3 and C4 generations of colchicine treated seeds. Amino acid analysis of whole grain meal of diploid Notch-2 and one strain of its autotetraploid revealed differences in the amino acid composition. The proportion of albumin in the diploid and the autotetraploid Notch-2 was higher by 21% and 45% respectively, in comparison to the parent 'N.P. 113', whereas the glutelin fraction was significantly higher in the autotetraploid. The autotetraploid, with increased glutelin and decreased prolamin, showed no increase in lysine. It is possible that the recessive high-lysine gene may be lacking dosage effect, resulting in no increase in lysine in the autotetraploid, whereas protein content, a polygenically controlled trait, is enhanced due to genome duplication.

Effect of the essential oils of garlic and onion on alimentary hyperlipemia.

SUMMARY: The effect of garlic and onion on alimentary hyperlipemia, induced by feeding 100 g butter, has been studied in 10 healthy subjects. The freshly extracted juice of 50 g of garlic or onion, as well as an equivalent amount of their ether-extracted essential oils, was administered randomly on four different days during a one-week period. Garlic and onion have a significant protective action against fat-induced increases in serum cholesterol and plasma fibrinogen and decreases in coagulation time and fibrinolytic activity. The essential oil fraction, which contains all the taste and odour, exactly duplicated the beneficial effects of whole garlic and onion. It is, therefore, concluded that the active principle of garlic and onion is the essential oil, which chemically is a combination of sulphur-containing compounds, mainly allyl propyl disulphide and diallyl disulphide.

The multiple-cluster mutation complex in mutagenesis with higher plants.

After treatment of dry and pre-soaked seeds of barley with gamma-rays, EMS, NEU and EI, the frequency of multiple mutations (multimutations) was higher with EMS and NEU treatment, while cluster mutations appeared in greater numbers following treatment with gamma rays and NEU. Pre-soaking the seeds led to a reduction in the frequency of total mutations, cluster mutations and multimutations. This has been explained as a result of the application of lower doses and the induction of mutations at a relatively later stage in ontogenetic development in the case of pre-soaked seeds.Some new mutation types in barley have been described and some of the old types have been given names representing the mutation characters more precisely.The compound mutation frequency of different seedling mutation types, when taken separately, was found to be independent of the mutagen employed and the stage of treatment. The size of mutated chimeras in M 1 plants, as indicated by the segregation ratio of mutants in M 2, was largest in albina, xantha, chlorina, albina-tigrina, chl-terminalis and eceriferum, and lowest in viridis, viridoalbina etc. This could be expected if the unstable premutations induced by mutagenic treatment are resolved into mutations at different intervals after their initiation, or it can be explained by the induction of dominant mutations, or lethal changes together with visible mutations.