Degradation and mechanism analysis of chloroxylenol in aqueous solution by gas-liquid discharge plasma combined with ozonation.

Gas-liquid discharge non-thermal plasma (NTP) coupled with an ozonation reactor was used to investigate the removal of a broad-spectrum antibacterial agent, chloroxylenol (PCMX), from aqueous solution. Under the same experimental conditions (discharge power of 50.25 W, the initial concentration of PCMX of 60 mg L-1, oxygen flow of 1.0 L min-1 and PCMX solution flow of 150 mL min-1), the PCMX degradation rates in the ozonation-only, NTP-only and NTP/O3 systems were 29.25%, 67.04% and 79.43%, respectively. Correspondingly, the energy efficiency has also been greatly improved, and increased to 0.45, 1.03 and 1.21 g kW-1 h-1. In addition, the effects of the initial concentration of PCMX, initial pH, the flow rate of oxygen, the addition of H2O2 and the addition of a radical scavenger on the degradation rate of PCMX were investigated in the NTP/O3 system. The degradation rate in acidic solutions was higher than that in alkaline solutions. During the removal process of PCMX, the rate of degradation was strongly increased with the addition of H2O2 and acutely decreased with the addition of the radical scavenger. Compared with deionized water the degradation rates of PCMX in secondary effluent were inhibited. Four main intermediates of PCMX degradation by the NTP/O3 system were identified by gas chromatography-mass spectrometry (GC-MS) and a possible degradation pathway of PCMX was proposed. The changes in toxicity of the PCMX solution during the NTP/O3 system oxidation process were also evaluated using bioluminescent bacteria and Quantitative Structure Activity Relationship (QSAR) models with the help of the ECOSAR software.

The Determinant of DNA Repair Pathway Choices in Ionising Radiation-Induced DNA Double-Strand Breaks.

Ionising radiation- (IR-) induced DNA double-strand breaks (DSBs) are considered to be the deleterious DNA lesions that pose a serious threat to genomic stability. The major DNA repair pathways, including classical nonhomologous end joining, homologous recombination, single-strand annealing, and alternative end joining, play critical roles in countering and eliciting IR-induced DSBs to ensure genome integrity. If the IR-induced DNA DSBs are not repaired correctly, the residual or incorrectly repaired DSBs can result in genomic instability that is associated with certain human diseases. Although many efforts have been made in investigating the major mechanisms of IR-induced DNA DSB repair, it is still unclear what determines the choices of IR-induced DNA DSB repair pathways. In this review, we discuss how the mechanisms of IR-induced DSB repair pathway choices can operate in irradiated cells. We first briefly describe the main mechanisms of the major DNA DSB repair pathways and the related key repair proteins. Based on our understanding of the characteristics of IR-induced DNA DSBs and the regulatory mechanisms of DSB repair pathways in irradiated cells and recent advances in this field, We then highlight the main factors and associated challenges to determine the IR-induced DSB repair pathway choices. We conclude that the type and distribution of IR-induced DSBs, chromatin state, DNA-end structure, and DNA-end resection are the main determinants of the choice of the IR-induced DNA DSB repair pathway.

New evidence and insight for abnormalities in early embryonic development after short-term spaceflight onboard the Chinese SJ-10 satellite.

During space travel, radiation and microgravity are recognized to be major hazardous factors in the overall health and well-being of astronauts. Although some efforts have been made to elucidate the effects of short-term space travel on the reproductive health of astronauts and multiple  other species in a variety of in vitro and in vivo studies, it is still unclear whether space travel can cause abnormal embryonic development or if it poses any reproductive risks. Recently, Lei et al. (2020) investigated the effects of short-term spaceflight onboard the Chinese SJ-10 satellite on murine preimplantation embryonic development. In the article, the authors claimed that the developmental abnormalities after short-term spaceflight onboard the SJ-10 satellite were attributed to space radiation and that these alterations in space was equivalent to those induced by a 2 mGy dose of gamma-rays in a ground-based facility. In this commentary, we discuss the possible space environmental factors and associated mechanisms that contribute to abnormalities in early embryonic development, and the potential health risks to mammals after short-term space travel. This commentary provides new evidence and a fresh perspective on whether and how short-term space travel poses potential reproductive risks in mammals.

Identification of potential radiation-responsive biomarkers based on human orthologous genes with possible roles in DNA repair pathways by comparison between Arabidopsis thaliana and homo sapiens.

Rapid and reliable ionization radiation (IR) exposure estimation has become increasingly important in environment due to the urgent requirement of medical evaluation and treatment in the event of nuclear accident emergency. Human DNA repair genes can be identified as important candidate biomarkers to assess IR exposure, while how to find the enough sensitive and specific biomarkers in the DNA repair networks is still challenged and not fully determined. The conserved features of DNA repair pathways may facilitate interdisciplinary studies that cross the traditional boundaries between animal and plant biology, with the aim of identifying undiscovered human DNA repair genes for potential radiation-responsive biomarkers. In this work, an in silico method of homologous comparison was performed to identify the human orthologues of A. thaliana DNA repair genes, and thereby to explore the sensitive and specific human radiation-responsive genes to evaluate the IR exposure levels. The results showed that a total of 16 putative candidate genes were involved in the human DNA repair pathways of homologous recombination (HR) and non-homologous end joining (NHEJ), and most of them were confirmed by previous experiments. Additionally, we analyzed the gene expression patterns of these 16 candidate genes in several human transcript microarray datasets with different IR treatments. The results indicated that most of the gene expression levels for these candidate genes were significantly changed under different radiation treatments. Based on these results, we integrated these putative human DNA repair genes into the DNA repair pathways to propose new insights of the HR and NHEJ pathways, which can also provide the potential targets for the development of radiation biomarkers. Notably, two putative DNA repair genes, named ERCC1 and ESCO2, were identified and were considered to be the sensitive and specific biomarkers in response to γ-ray exposures.

In situ measurement of the two-dimensional temperature field of a dual-jet direct-current arc plasma.

In this paper, a real time method for an in situ measurement of the two-dimensional (2-D) temperature filed of thermal plasmas is developed with the combination of the visible image processing technique and the spectroscopic line-ratio method at two specified wavelengths. After the calibration of the gray scale values of the recorded images with the CCD cameras by the emission intensity received using a spectrometer, the 2-D temperature field of the plasma arc-jet can be obtained conveniently based on the derived gray scale values of the CCD images at two specified wavelengths and the formula similar to that of spectroscopic line-ratio method. The experimental results show that the obtained temperature fields of the plasma arc-jet at different times are qualitatively reasonable and consistent with the modeling result. This newly developed method can be employed to measure the transient temperature fields of the plasmas with fluctuations during discharges effectively.

Note: Stability control of intermediate frequencies of a three laser far-infrared polarimeter-interferometer system.

Stability of the intermediate frequency (IF) in the far-infrared polarimeter-interferometer diagnostic system is critically important for the long pulse discharge experiments on the EAST tokamak. In this note, a real-time remote/local IF stability control system is described. The measured plasma parameters, including the Faraday rotation angle, electron density, lower hybrid wave, and plasma current, are obtained with the aid of this newly developed IF stability control system.

Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool.

Developing rapid and diverse microbial mutation tool is of importance to strain modification. In this review, a new mutagenesis method for microbial mutation breeding using the radio-frequency atmospheric-pressure glow discharge (RF APGD) plasma jets is summarized. Based on the experimental study, the helium RF APGD plasma jet has been found to be able to change the DNA sequences significantly, indicating that the RF APGD plasma jet would be a powerful tool for the microbial mutagenesis with its outstanding features, such as the low and controllable gas temperatures, abundant chemically reactive species, rapid mutation, high operation flexibility, etc. Then, with the RF APGD plasma generator as the core component, a mutation machine named as atmospheric and room temperature plasma (ARTP) mutation system has been developed and successfully employed for the mutation breeding of more than 40 kinds of microorganisms including bacteria, fungi, and microalgae. Finally, the prospect of the ARTP mutagenesis is discussed.

[Operating conditions for the rapid mutation of the oleaginous yeast by atmospheric and room temperature plasmas and the characteristics of the mutants].

To obtain oleaginous yeast mutants with improved lipid production and growth rates, an atmospheric and room temperature plasma (ARTP) jet was used with a 96-well plate for high throughput screening. Mutants with changes in growth rates and lipid contents were obtained. At a lethality rate of 99%, the positive mutation rate of the yeast cells was 27.2% evaluated by the growth rates of the mutants and the comparison with the wild strain. The fermentation in a medium composed of yeast extract (10 g/L), peptone (10 g/L) and D-glucose (20 g/L) resulted in the lipid yield of the mutant (C4) with 4.07% (W/W) compared with that of the wild strain (1.87%).

[Spectroscopic methodology for the measurement of tokamak plasma].

The states and the distributions of the plasma in the tokamak can be obtained by measuring the spectrum. Since there are many different objects to be measured, many different apparatuses are used to get a better result. Some methods for the measurement are listed in this article. Also the measuring range of each method and some results are listed. On the other hand, a new method was developed according to the conditions of the lab.