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2.
Genet Test Mol Biomarkers ; 26(11): 543-549, 2022 Nov.
Article in English | MEDLINE | ID: mdl-36378841

ABSTRACT

Background: Previous studies have reported conflicting results regarding the potential association between the risk of Parkinson's disease (PD) and the single nucleotide polymorphism, rs11558538 (Thr105Ile), in the histamine N-methyltransferase (HNMT) gene. We performed a systematic review and meta-analysis to improve our understanding of the association between them. Methods: We systematically searched several online databases to identify relevant studies regarding the association between rs11558538 and PD. We extracted data on the frequencies of genotypes (Thr/Thr, Thr/Ile, and Ile/Ile) and alleles (Thr and Ile) at the rs11558538 locus in patients with PD and healthy controls. Associations between genotype and PD risk were assessed in terms of odds ratios (OR) and 95% confidence intervals (CI). Results: The final meta-analysis included six case-control studies and data from the International Parkinson's Disease Genomics Consortium (IPDGC) data base on the association between HNMT rs11558538 and PD, involving 22,855 patients and 65,367 controls. Among the studies, substantial heterogeneity was observed (I2 = 84.42 for genotype and I2 = 73.39 for allele). Both the Ile (log OR: -0.31; 95% CI: -0.5 to -0.12; p < 0.001) and Thr/Ile+Ile genotypes (log OR: -0.32; 95% CI: -0.55 to -0.08; p < 0.001) were associated with a decreased risk of sporadic PD across all study populations. Subgroup analysis showed the protective effect of Thr/Ile+Ile genotypes in non-Chinese cohorts (log OR: -0.66; 95% CI: -0.67 to -0.04; p < 0.001) but not in Chinese cohorts (log OR: -0.26; 95% CI: -0.63 to 0.11; p = 0.13). Conclusion: Our findings suggest that the HNMT rs11558538T polymorphism may protect against PD, particularly in patients from the United States and Europe.


Subject(s)
Histamine N-Methyltransferase , Parkinson Disease , Humans , Histamine N-Methyltransferase/genetics , Parkinson Disease/genetics , Genetic Predisposition to Disease/genetics , Polymorphism, Single Nucleotide/genetics , Asian People/genetics
3.
Front Chem ; 10: 981579, 2022.
Article in English | MEDLINE | ID: mdl-36311425

ABSTRACT

In this article, long fiber reinforced polybutylene terephthalate (LGF/PBT/DOPO-HQ) flame-retardant composites were prepared using 10-(2,5-dihydroxy phenyl)-10H-9-oxa-10-phosphaphenanthrene-10-oxide (DOPO-HQ) as the conjugated flame-retardant. The effects of different flame-retardant contents on the combustion properties of the composites were investigated. The results showed that after adding 14% of DOPO-HQ, the flame-retardant effect of the composite reached the V-0 level of UL-94 fire rating with an ultimate oxygen index (LOI) of 26.4%. The average heat release rate (Av-HRR), peak heat release rate (PHRR), and total heat release rate (THR) decreased by 45.9, 56.5, and 32.6%, respectively. This shows that LGF/PBT/DOPO-HQ composite has good flame-retardant properties. Meanwhile, the flame-retardant mechanism of cohesive phase and gas-phase synergy during the combustion of flame retardants was analyzed by carbon layer morphology and dynamic thermal decomposition.

4.
Front Chem ; 10: 956322, 2022.
Article in English | MEDLINE | ID: mdl-35923254

ABSTRACT

The conjugated flame retardants have rarely been studied. A conjugate flame-retardant 4, 4'-{1″, 4″-phenylene-bis [amino- (10‴-oxy10‴-hydro-9‴-hydrogen- 10‴λ5-phosphaphenanthrene-10″-yl)-methyl]}-diphenol (P-PPD-Ph) was synthesized and added into the polylactic acid (PLA) matrix. The P-PPD-Ph-conjugated flame-retardant structure was tested by FTIR, 1H, and 31P NMR analysis. The thermal and rheological properties of PLA/P-PPD-PH-conjugated flame-retardant composites were investigated. The results showed that P-PPD-Ph-conjugated flame retardant affects PLA/P-PPD-PH-conjugated flame-retardant composites for promoting the formation of a carbon layer when the P-PPD-Ph-conjugated flame-retardant content was 15% and the residual carbon ratio for PLA/P-PPD-PH-conjugated flame-retardant composites increased by 4.2%.

5.
Front Chem ; 10: 933716, 2022.
Article in English | MEDLINE | ID: mdl-35774856

ABSTRACT

A bridged 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide derivative (DiDOPO) with conjugated structure was utilized as a novel conjugated flame retardant, Polypropylene(PP)/DiDOPO conjugated flame retardant composites were papered by being melt-extruding with a twin-screw extruder. The flame retardant efficiency of PP/DiDOPO conjugated flame retardant composites were investigated by cone calorimetry, limiting oxygen index (LOI), vertical burning test (UL-94). Besides, the rheological behavior of PP/DiDOPO conjugated flame retardant composites are measured by ARES rheometer. The results showed that when the content of DiDOPO with conjugated structure was 16 wt%, the LOI values of PP/DiDOPO conjugated flame retardant composites was 24%, and PP/DiDOPO conjugated flame retardant composites reaches V-0 grade. The heat release rate (HRR), total heat release rate (THR) and CO2 of PP/DiDOPO conjugated flame retardant composites decreased, so PP/DiDOPO conjugated flame retardant composites had excellent flame retardant effect. Rheological analysis results indicated that DiDOPO with conjugated structure suppressed the melt dripping of PP/DiDOPO conjugated flame retardant composites by enhancing the melt stability. The results showed that the DiDOPO with conjugated structure can significantly enhance the flame retardancy effect of PP/DiDOPO conjugated flame retardant composites. In addition, the materials PP/DiDOPO might be with low conductivity and charge transport mobility.

6.
Front Chem ; 10: 894112, 2022.
Article in English | MEDLINE | ID: mdl-35646831

ABSTRACT

The DOPO derivative-conjugated flame retardant 4, 4'-{1'', 4'' - phenylene - bis [amino - (10‴ - oxy -10‴-hydro-9‴-hydrogen-10‴ λ5 -phosphaphenanthrene-10''-yl)-methyl]}-diphenol (P-PPD-Ph) with two hydroxyl groups was synthesized. Polylactic acid conjugated flame-retardant composites with P-PPD-Ph were papered by using a twin-screw extruder. The flame-retardant properties of polylactic acid-conjugated flame-retardant composites were investigated. The flame-retardant properties of PLA-conjugated flame-retardant composites were characterized by the limiting oxygen index (LOI) and the vertical burning test (UL94). The results showed that the PLA-conjugated flame-retardant composites achieved a V-0 rating (UL-94, 3.2 mm) when the conjugated flame retardant was added at 5 wt%, and increase in LOI value from 22.5% to 31.4% relative to composites without added conjugated flame retardant. The flame-retardant mechanism of PLA-conjugated flame-retardant composites were further studied by TG-FTIR, the results showed that the P-PPD-Ph promoted the PLA-conjugated flame-retardant composites to decompose and also released fragments with quenching and dilution, which suggests that P-PPD-Ph for PLA-conjugated flame-retardant composites mainly play a role of the gas-phase flame retardant.

7.
Ann Palliat Med ; 10(10): 10797-10803, 2021 10.
Article in English | MEDLINE | ID: mdl-34763441

ABSTRACT

BACKGROUND: This study investigated the advantages and disadvantages of contrast media administration by gravity drip and manual push injection during cholangiography. METHODS: A total of 100 patients who presented to the Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, for a cholangiography between June 2019 to June 2020 were enrolled in this study. Patients were randomly divided into 2 treatment groups. One group of patients with manual injection of contrast (the N group, n=50), received the contrast agent via the traditional manual injection method whereby the doctor injects 50 mL of prepared contrast agent into the right side of the patient while continuously observing the effects on the bile duct. The other group of patients with gravity drip administration of contrast media (the O group, n=50), received the contrast agent via gravity drip at a rate of 80 drops per minute, and both clinicians and radiologists monitored the entire cholangiography process from a safe distance. Patients were followed up and angiographic satisfaction was assessed after two weeks. RESULTS: All 100 patients completed cholangiography without allergic reaction to the contrast medium. In the traditional injection group (N group), nine patients experienced upper abdominal discomfort with nausea, abdominal pain, chills, high fever, and other symptoms, and residual gallstones were observed in 12 patients. In patients in the gravity drip group (O group), four patients felt upper abdominal discomfort accompanied by nausea, abdominal pain, chills, high fever, and other symptoms, with residual gallstones detected in six patients. CONCLUSIONS: Patients who underwent gravity drip cholangiography had significantly reduced adverse reactions compared to patients who underwent traditional manual infusion cholangiography. Furthermore, gravity drip cholangiography resulted in clearer images and reduced X-ray exposure for medical staff. Thus, increased implementation of gravity drip cholangiography in the clinical setting should be considered. TRIAL REGISTRATION: Chinese Clinical Trial Registry ChiCTR1800018202.


Subject(s)
Cholangiography , Humans
8.
3 Biotech ; 11(6): 258, 2021 Jun.
Article in English | MEDLINE | ID: mdl-33987074

ABSTRACT

To explore the potential function of miR-9-5p in wear-particle-induced osteoclastogenesis, we examined the expression of SIRT1 and miR-9-5p in particle-induced osteolysis (PIO) mice calvariae and polyethylene (PE)-induced RAW 264.7 cells and found that SIRT1 expression was downregulated while miR-9-5p expression was upregulated in both models. We then verified that miR-9-5p targets SIRT1. miR-9-5p was found to promote PE-induced osteoclast formation from RAW 264.7 cells by tartrate-resistant acid phosphatase staining and detection of osteoclast markers, and miR-9-5p activation of the SIRT1/NF-kB signaling pathway was found in cells by detecting the expression of SIRT1/NF-kB pathway-related proteins and rescue assays. In conclusion, we found that miR-9-5p activated the SIRT1/NF-κB pathway to promote wear-particle-induced osteoclastogenesis. miR-9-5p may be a useful therapeutic target for PIO remission and treatment.

9.
Trials ; 20(1): 399, 2019 Jul 04.
Article in English | MEDLINE | ID: mdl-31272502

ABSTRACT

BACKGROUND: The morbidity of knee arthritis is increasing among aged people and total knee arthroplasty has been its mainstream treatment to date. Postoperative rehabilitation is an important part of the procedure. However, the intense pain during the functional exercise involved has always been a challenge for both patients and health care professionals. The aim of this study is to test the analgesic effect of a mixture of nitrous oxide/oxygeb (1:1) inhalation for patients who are doing functional exercise 1 month after total knee arthroplasty. METHODS/DESIGN: This double-blind, randomized, placebo-controlled study will be implemented in the Rehabilitation Department in the General Hospital of Ningxia Medical University. Patients aged between 50 and 75 years who underwent a primary unilateral total knee arthroplasty are eligible for inclusion. The key exclusion criteria include: epilepsy, pulmonary embolism, intestinal obstruction, aerothorax. The treatment group (A) will receive a pre-prepared nitrous oxide/oxygen mixture plus conventional treatment (no analgesics), and the control group (B) will receive oxygen plus conventional treatment (no analgesics). Patients, physicians, therapists, and data collectors are all blind to the experiment. Assessments will be taken immediately after functional exercise begins (T0), 5 min (T1) after functional exercise begins, and 5 min after functional exercise has finished (T2). Patients will be randomly allocated between a treatment group (A) and a control group (B) in a ratio of 1:1. Primary outcome, including pain severity in the procedure, will be taken for each group. Secondary outcomes include blood pressure, heart rate, oxygen saturation, side effects, knee joint range of motion, Knee Society Score (KSS), rescue analgesia need, and satisfaction from both therapists and patients. DISCUSSION: This study will focus on exploring a fast and efficient analgesic for patients who are doing functional exercise after total knee arthroplasty. Our previous studies suggested that the prefixed nitrous oxide/oxygen mixture was an efficacious analgesic for the management of burn-dressing pain and breakthrough cancer pain. The results of this study should provide a more in-depth insight into the effects of this analgesic method. If this treatment proves successful, it could be implemented widely for patients doing functional exercise in the rehabilitation department. TRIAL REGISTRATION: ChiCTR-INR-17012891 . Registered on 6 October 2017.


Subject(s)
Analgesics, Non-Narcotic/administration & dosage , Arthralgia/prevention & control , Arthritis/surgery , Arthroplasty, Replacement, Knee/rehabilitation , Exercise Therapy , Knee Joint/surgery , Nitrous Oxide/administration & dosage , Oxygen Inhalation Therapy , Pain, Postoperative/prevention & control , Aged , Analgesics, Non-Narcotic/adverse effects , Arthralgia/diagnosis , Arthralgia/etiology , Arthralgia/physiopathology , Arthritis/diagnosis , Arthritis/physiopathology , Arthroplasty, Replacement, Knee/adverse effects , China , Double-Blind Method , Exercise Therapy/adverse effects , Female , Humans , Knee Joint/physiopathology , Male , Middle Aged , Nitrous Oxide/adverse effects , Oxygen Inhalation Therapy/adverse effects , Pain Measurement , Pain, Postoperative/diagnosis , Pain, Postoperative/etiology , Pain, Postoperative/physiopathology , Randomized Controlled Trials as Topic , Time Factors , Treatment Outcome
10.
Biomed Pharmacother ; 104: 300-306, 2018 Aug.
Article in English | MEDLINE | ID: mdl-29775898

ABSTRACT

BACKGROUND: Sirtuin 1 (SIRT1) downregulation has been found to be induced by wear particles in aseptic prosthesis loosening (APL). Osteoclastogenesis and osteoclast activation are the main pathological factors associated with APL. However, whether SIRT1 downregulation contributes to the formation and activation of osteoclasts through the induction of endoplasmic reticulum (ER) stress is unclear. METHODS: To address this, an osteolysis mouse model was used in which animals were treated with the SIRT1 activator, resveratrol (RES), or an ER stress inhibitor, 4-PBA, for two weeks. Osteolysis, osteoclastogenesis, and morphologic alteration of calvariae were observed by toluidine blue, TRAP, and H&E staining. SIRT1 expression and ER stress were evaluated by western blot analysis. In vitro, mouse macrophage RAW 264.7 cells were treated with polyethylene (PE) particles alone or combined with either RES or 4-PBA, and SIRT1 expression and ER stress were measured using western blot assays. Osteoclast differentiation was determined through TRAP staining. Osteoclast activation was evaluated by culturing osteoclast cells on bone slices followed by toluidine blue staining. Mechanistically, osteoclastogenesis-related MAPK activation, NFATc1 and c-Fos expression, and NF-κB translocation were determined. RESULTS: Both in vivo and in vitro experimental results indicated that PE particles induced SIRT1 downregulation and enhanced ER stress. SIRT1 activator RES and ER stress inhibitor 4-PBA significantly suppressed PE particle-induced osteoclast differentiation and osteolysis. In vitro experimental results showed that 4-PBA suppressed PE particle-induced ERK1/2, p38, and JNK activation, NFATc1 and c-Fos upregulation, as well as NF-κB p65 nucleus translocation. CONCLUSIONS: PE particle-induced downregulation of SIRT1 enhances ER stress and promotes osteoclast proliferation and bone resorption through regulation of c-Fos, NFATc1, and the MAPK and NF-κB signaling pathways.


Subject(s)
Down-Regulation/genetics , Endoplasmic Reticulum Stress/genetics , Osteogenesis/genetics , Osteolysis/genetics , Sirtuin 1/genetics , Animals , Cell Differentiation/genetics , Cell Line , Disease Models, Animal , Female , Mice , Mice, Inbred C57BL , Mitogen-Activated Protein Kinases/genetics , NF-kappa B/genetics , NFATC Transcription Factors/genetics , Osteoclasts , Proto-Oncogene Proteins c-fos/genetics , RAW 264.7 Cells , Signal Transduction/genetics
12.
Sheng Wu Gong Cheng Xue Bao ; 32(12): 1715-1726, 2016 Dec 25.
Article in Chinese | MEDLINE | ID: mdl-29034639

ABSTRACT

To obtain sufficient purified and active fusion protein-hepatocyte-targeting peptide-human endostatin (HTP-rES), we studied the growth curve and the optimal induction timing of BL21/pET21b-HTP-rES. Different conditions of pH value, induction time, induction concentration and induction temperature were optimized by univariate analysis. After washing, refolding and purifying, the activity of fusion protein was identified by flow cytometry and 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT). Results show that the logarithmic growth phase of BL21/pET21b-HTP-rES was from 1.5 h to 3.5 h, the optimum expression conditions were pH 8.0, 0.06 mmol/L IPTG, at 42 ℃ for 5 h. The purity of inclusion bodies was up to 60% after washing. The purity of target protein was more than 95% after refolding and purification. Our findings provide the foundation for further biological activity and drug development.


Subject(s)
Drug Delivery Systems , Endostatins/pharmacology , Hepatocytes/drug effects , Escherichia coli , Humans , Inclusion Bodies , Peptides/pharmacology , Recombinant Fusion Proteins
13.
Protein Pept Lett ; 22(5): 470-9, 2015.
Article in English | MEDLINE | ID: mdl-25731590

ABSTRACT

A missense mutation of Aspartic to Asparagine acid in 113 position of liver-targeting peptide CSP I-plus modified rEndostatin (rES-CSP) happened unexpectedly results in the changes of protein secondary structure and a reduced bioactivity. With the aim to clarify the structure-function relationships featuring the fuse protein rES-CSP, the three-dimensional structural models of wild-type and mutant D113NrES-CSP were constructed by template-based modeling approach. To evaluate the effect of the single mutation on rES-CSP stability, the molecular dynamic simulation was used to reveal the structural and dynamic characteristics. Analysis on the bioactivity were conducted using a number of validated in vitro assays including proliferation, migration, cell cycle and apoptosis in HepG2 cells. Results showed that the mutant rES-CSP reduce the stability and loss of function, and the wild-type rES-CSP could both bind to the normal liver cells Chang's and the hepatoma cells HepG2 but significantly higher than non-targeted rEndostatin. rESCSP could inhibit the proliferation of hepatoma cells in a dose-dependent manner, and increase the proportion of G1 phase, reduce the proportion of S phase, promote the apoptosis on hepatoma cells. These results make a further complement of the mechanisms by which the fuse protein rES-CSP would provide a feasible and convenient approach to produce liver-targeting drugs for treatment of the liver disease.


Subject(s)
Carcinoma, Hepatocellular/drug therapy , Endostatins/chemistry , Endostatins/pharmacology , Liver Neoplasms/drug therapy , Peptides/chemistry , Peptides/pharmacology , Amino Acid Sequence , Apoptosis/drug effects , Carcinoma, Hepatocellular/metabolism , Carcinoma, Hepatocellular/pathology , Cell Cycle/drug effects , Cell Movement/drug effects , Cell Proliferation/drug effects , Endostatins/genetics , Hep G2 Cells , Humans , Liver/drug effects , Liver/metabolism , Liver/pathology , Liver Neoplasms/metabolism , Liver Neoplasms/pathology , Models, Molecular , Molecular Sequence Data , Mutation , Peptides/genetics , Protein Conformation , Protozoan Proteins/chemistry , Protozoan Proteins/genetics , Protozoan Proteins/pharmacology , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/pharmacology
14.
World J Surg Oncol ; 12: 285, 2014 Sep 13.
Article in English | MEDLINE | ID: mdl-25216551

ABSTRACT

BACKGROUND: This study aimed at identifying risk factors of recurrence for completely resected pathologic T1aN0M0 lung adenocarcinomas. METHODS: We reviewed the records of 177 T1aN0M0 invasive adenocarcinoma patients, and re-classified achieved surgical specimens according to the new International Association for the Study of Lung Cancer, American Thoracic Society, and European Respiratory Society (IASLC/ATS/ERS) lung adenocarcinoma classification. Impact on recurrence-free survival (RFS) for age, gender, smoking history, lymphovascular invasion (LVI) and new classification was analyzed by log-rank test and Cox regression. Two existing prognostic grouping schemes of new classification were compared, and subsequently, the correlation of high-grade group in the better prognostic grouping model with clinical data was investigated statistically. RESULTS: The 5-year recurrence-free rate was 83.7%. The LVI and new adenocarcinoma classification were significantly associated with 5-year RFS (P = 0.012; P = 0.022, respectively). The designation of papillary predominant subtype in the low-grade group, along with lepidic- and acinar predominant subtype had more prognostic significance than the model of combining papillary-, solid- and micropapillary predominant subtypes as the high-grade group (P = 0.005 versus P = 0.181). This high-grade group has increased risk of recurrence in a multivariate Cox regression (adjusted HR 2.815, 95% CI: 1.239 to 6.397, P = 0.013), and is associated significantly more with male gender (adjusted OR 2.214, 95% CI: 1.050 to 4.668, P = 0.037), and, with borderline significance, the presence of LVI (adjusted OR 2.091, 95% CI: 0.938 to 4.662, P = 0.071). CONCLUSIONS: Our results showed that the solid- and micropapillary predominant subtype of IASLC/ATS/ERS classification remains the only risk factor for post-operative recurrence of T1aN0M0 adenocarcinomas, suggesting that they can be indicators of aggressive tumor behaviors.


Subject(s)
Adenocarcinoma/complications , Carcinoma, Non-Small-Cell Lung/complications , Carcinoma, Papillary/complications , Lung Neoplasms/complications , Neoplasm Recurrence, Local/diagnosis , Adenocarcinoma/mortality , Adenocarcinoma/pathology , Adenocarcinoma/surgery , Adult , Aged , Aged, 80 and over , Carcinoma, Non-Small-Cell Lung/mortality , Carcinoma, Non-Small-Cell Lung/pathology , Carcinoma, Non-Small-Cell Lung/surgery , Carcinoma, Papillary/mortality , Carcinoma, Papillary/pathology , Carcinoma, Papillary/surgery , Female , Follow-Up Studies , Humans , Lung Neoplasms/mortality , Lung Neoplasms/pathology , Lung Neoplasms/surgery , Male , Middle Aged , Neoplasm Grading , Neoplasm Recurrence, Local/etiology , Neoplasm Recurrence, Local/mortality , Neoplasm Staging , Prognosis , Prospective Studies , Retrospective Studies , Risk Factors , Survival Rate
15.
Zhonghua Fu Chan Ke Za Zhi ; 49(3): 204-7, 2014 Mar.
Article in Chinese | MEDLINE | ID: mdl-24820306

ABSTRACT

OBJECTIVE: To investigate the clinicopathologic characteristics of premalignant and malignant endometrial polyps (EP) in premenopausal and postmenopausal women. METHODS: A retrospective analysis was conducted in 42 cases of premalignant and malignant EP from 1993 to 2012. Polyps were classified into premenopausal (group A, 10 cases) and menopausal (group B, 32 cases), including 26 cases of endometrioid adenocarcinoma, 4 of clear cell carcinoma, 9 of serous adenocarcinoma, and 3 of atypical hyperplasia. RESULTS: The prevalence rate of premalignant and malignant EP was 1.42% (42/2 965), the prevalence rate of malignancy in postmenopausal and postmenopausal women was 0.48% (10/2 064) and 3.55% (32/901), respectively. The mean size of EP was (1.6 ± 0.8) cm, abnormal uterine bleeding was positive in 90% (38/42) of cases. The EP pathological diagnosis showed all were endometrioid adenocarcinoma in group A, while there were 4 of clear cell carcinoma, 9 of serous adenocarcinoma in group B. The mean size of EP was (1.1 ± 0.6) and (1.7 ± 0.9) cm in group A and B respectively (P < 0.05). According to immunohistochemistry, all cases of group A were ER positive, but 41% (11/27) of group B were ER negative (P = 0.059). The PR positive rate was 8/9 and 56% (15/27) in group A and B, respectively (P = 0.169). CONCLUSIONS: The risk of the EP malignancy rate is higher, while ER, PR positive rate are lower in postmenopausal women. Postmenopausal EP, especially accompanied by abnormal uterine bleeding and large polyps should be removed as soon as possible.


Subject(s)
Carcinoma, Endometrioid/pathology , Cystadenocarcinoma, Serous/pathology , Endometrial Neoplasms/pathology , Polyps/pathology , Aged , Carcinoma, Endometrioid/epidemiology , Cystadenocarcinoma, Serous/epidemiology , Endometrial Neoplasms/epidemiology , Endometrium/pathology , Female , Humans , Hysteroscopy , Middle Aged , Neoplasm Staging , Polyps/epidemiology , Postmenopause , Premenopause , Prevalence , Retrospective Studies , Risk Factors
16.
Tumori ; 97(5): 585-9, 2011.
Article in English | MEDLINE | ID: mdl-22158488

ABSTRACT

AIMS AND BACKGROUND: Epithelioid angiosarcoma is a rare histopathologic variant of angiosarcoma characterized by an epithelioid morphology. This subset can histologically mimic non-vascular neoplasms and impose serious challenges in reaching a correct diagnosis, especially in the context of limited tissue sampling (e.g., needle core biopsy). To improve recognition of epithelioid angiosarcoma - and the spectrum of morphologic diversity associated with this rare variant - and to avoid a misdiagnosis, we describe the clinical, histopathologic, and immunohistochemical findings of cases of epithelioid angiosarcoma diagnosed at our institution. METHODS AND STUDY DESIGN: Seven cases of epithelioid angiosarcoma with appropriate pathologic material were identified from our archives. Immunohistochemistry was used to detect the expression of CD31, CD34, Factor VIII, cytokeratin, epithelial membrane antigen, vimentin, HMB45, CD1a, CD68, lysozyme, CD45, desmin, and smooth muscle actin in all cases. Follow-up information was obtained by reviewing medical records or by direct communication with family members. RESULTS: The lesions involved the bone (n = 4) and soft tissues (n = 3). Microscopically, all tumors had a predominantly diffuse growth pattern, with a focal nested architecture in 6 cases, which closely mimicked metastatic carcinoma. The initial biopsy was performed in 2 of 6 patients and revealed the presence of a malignant neoplasm suggestive of metastatic carcinoma. Immunohistochemically, the epithelioid endothelial cells usually showed strong reactivity for CD31 (7/7), variable or focal positive staining for CD34 (5/7), Factor VIII (4/7), cytokeratin (6/7), epithelial membrane antigen (2/7), vimentin (7/7), and CD68 (3/7). In contrast, they were negative for CD1a, HMB45, lysozyme, CD45, desmin, and smooth muscle actin. Three patients died of disease within one year of the diagnosis, 2 patients developed local recurrence or metastases, and another 2 were disease-free at this writing. CONCLUSIONS: With any unusual epithelioid neoplasm displaying some or all of the morphologic features described above, epithelioid angiosarcoma should be included in the differential diagnosis. In such an instance, endothelial markers should be incorporated in the immunohistochemical analysis to avoid misdiagnosis, particularly with limited sampling.


Subject(s)
Biomarkers, Tumor/analysis , Bone Neoplasms/chemistry , Bone Neoplasms/pathology , Hemangiosarcoma/chemistry , Hemangiosarcoma/pathology , Actins/analysis , Adult , Aged , Aged, 80 and over , Antibodies, Monoclonal/analysis , Antibodies, Monoclonal, Humanized , Antigens, CD/analysis , Antigens, CD1/analysis , Antigens, CD34/analysis , Antigens, Differentiation, Myelomonocytic/analysis , Bone Neoplasms/therapy , Desmin/analysis , Female , Hemangiosarcoma/secondary , Hemangiosarcoma/therapy , Humans , Immunohistochemistry , Keratins/analysis , Male , Melanoma-Specific Antigens/analysis , Middle Aged , Muramidase/analysis , Platelet Endothelial Cell Adhesion Molecule-1/analysis , Soft Tissue Neoplasms/chemistry , Soft Tissue Neoplasms/pathology , Vimentin/analysis , gp100 Melanoma Antigen
17.
Chin J Cancer ; 30(9): 645-54, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21880186

ABSTRACT

Neoadjuvant chemotherapy (NACT), which can reduce the size and therefore increase the resectability of tumors, has recently evolved as a treatment for locally advanced cervical cancer. NACT has been reported to decrease the risk of pathologic factors related to prognosis of cervical cancer. To further assess the effects of NACT on surgery and the pathologic characteristics of cervical cancer, we reviewed 110 cases of locally advanced cervical cancer treated with radical hysterectomy with or without NACT at the People's Hospital of Peking University between January 2006 and December 2010. Of 110 patients, 68 underwent platinum-based NACT prior to surgery (NACT group) and 42 underwent primary surgery treatment (PST group). Our results showed 48 of 68 (70.6%) patients achieved a complete response or partial response to NACT. Estimated blood loss, operation time, and number of removed lymph nodes during surgery, as well as complication rates during and after surgery were not significantly different between the NACT group and the PST group. The rates of deep stromal invasion, positive parametria, positive surgical vaginal margins, and lymph node metastasis were not significantly different between the two groups. However, the rate of lymph-vascular space involvement (LVSI) was significantly lower in the NACT group than in the PST group (P = 0.021). In addition, the response rate of NACT was significantly higher in the patients with chemotherapeutic drugs administrated via artery than via vein. Our results suggest that NACT is a safe and effective treatment for locally advanced cervical cancer and significantly decreases the rate of LVSI.


Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Carcinoma, Squamous Cell/drug therapy , Neoadjuvant Therapy , Uterine Cervical Neoplasms/drug therapy , Adult , Bleomycin/therapeutic use , Carcinoma, Squamous Cell/pathology , Carcinoma, Squamous Cell/surgery , Cisplatin/therapeutic use , Female , Humans , Hysterectomy/methods , Ifosfamide/therapeutic use , Lymph Node Excision , Lymphatic Metastasis , Middle Aged , Neoplasm Invasiveness , Neoplasm Staging , Remission Induction , Retrospective Studies , Uterine Cervical Neoplasms/pathology , Uterine Cervical Neoplasms/surgery
19.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 43(12): 756-60, 2008 Dec.
Article in Chinese | MEDLINE | ID: mdl-19134356

ABSTRACT

OBJECTIVE: To investigate the roles of matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP) in dentinogenic ghost cell tumor (DGCT) and ghost cell odontogenic carcinoma (GCOC). METHODS: The expressions of MMP-2, MMP-9, MMP-14, TIMP-1 and TIMP-2 were examined in 15 DGCT cases and 9 GCOC cases by immunohistochemistry. Their mRNA expression in one DGCT case and one GCOC case were investigated by RT-PCT.MMP-2 and MMP-9 protein activities in the two cases were analyzed by gelatin zymography. RESULTS: MMP-9 and TIMP-1 expressions elevated greatly in GCOC, and there was a significant difference (P < 0.05) in TIMP-1 expression between GCOC and DGCT.Pro-MMP-9, MMP-9 activated form, pro-MMP-2, and MMP-2 activated forms were detected in the GCOC case, while pro-MMP-9 and MMP-9 activated form were very faint in the DGCT case. The mRNA level of MMP-9 elevated obviously in the GCOC case, which was similar to that of TIMP-1. CONCLUSIONS: The elevated expression of MMP-9 and TIMP-1 may influence the behaviour of GCOC.


Subject(s)
Dentin , Mandibular Neoplasms/metabolism , Matrix Metalloproteinases/metabolism , Odontogenic Tumors/metabolism , Tissue Inhibitor of Metalloproteinases/metabolism , Adolescent , Adult , Aged , Child , Humans , Mandibular Neoplasms/pathology , Middle Aged , Odontogenic Tumors/pathology , Young Adult
20.
Quintessence Int ; 38(2): 103-10, 2007 Feb.
Article in English | MEDLINE | ID: mdl-17263149

ABSTRACT

OBJECTIVE: To identify the clinicopathologic features of epulides in West China and to compare these data with those of previous studies from other countries. METHOD AND MATERIALS: Demographics; clinical data including gender, age, and chief complaints of the patients; and the type, size and location, duration, diagnosis, and histologic features of the lesion were studied from among biopsy specimens and clinical records at West China College of Stomatology from January 1951 to July 2005. RESULTS: A total of 2,439 epulides were identified. Epulides found were peripheral fibroma (PF: 1,489, 61.05%), peripheral ossifying fibroma (POF: 431, 17.67%), pyogenic granuloma (PG: 482, 19.76%), and peripheral giant cell granuloma (PGCG: 37, 1.52%). The total ratio of males to females was 1:1.40. Specifically, the ratios were 1:1.31 for PF, 1:1.99 for PG, and 1:1.47 for PGCG (all P < .001). The peak incidence of epulides fell in the third to sixth decade of life overall, the fifth to sixth decade for PF and POF, and the third to fourth decade for PG. CONCLUSIONS: Among the 4 kinds of reactive lesions, PF had the highest incidence and PG had the lowest incidence in Chinese patients. There were also differences in type, sex, age, location, duration, and histologic features: PF was the most common type; all of the epulides except PGCG showed a predilection for females; PG was more prevalent among young patients; and POF lasted much longer than other lesions. Differences in the distribution of the 4 types of lesion were also found among various ethnic groups.


Subject(s)
Bone Neoplasms/epidemiology , Fibroma, Ossifying/epidemiology , Gingival Diseases/epidemiology , Granuloma, Giant Cell/epidemiology , Granuloma, Pyogenic/epidemiology , Adolescent , Adult , Age Distribution , Aged , Aged, 80 and over , Bone Neoplasms/pathology , Chi-Square Distribution , Child , Child, Preschool , China/epidemiology , Female , Fibroma, Ossifying/pathology , Gingival Diseases/pathology , Granuloma, Giant Cell/pathology , Granuloma, Pyogenic/pathology , Humans , Male , Middle Aged , Retrospective Studies , Sex Distribution
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