ABSTRACT
MicroRNA 144 (miR-144), a small non-coding RNA, is frequently dysregulated in human several tumour progression, but its role and the underlying mechanisms in hepatocellular carcinoma (HCC) is poorly investigated. In the present study, the expression of miR-144 was firstly analysed in datasets derived from GSE21362 and TCGA, and then detected in HCC tissues and cell lines by quantitative RT-PCR (qRT-PCR) analysis. MiR-144 was shown to be significantly down-regulated in HCC tissues and cell lines. Subsequently, overexpression of miR-144 was transfected into HCC cell lines so as to investigate its biological function, including MTT, colony formation, and transwell assays. Gain of function assay revealed miR-144 remarkably inhibited cell proliferation, migration and invasion. In addition, bioinformatical analysis and luciferase reporter assay identified ZFX as a novel target of miR-144 in HCC cells, as confirmed by qRT-PCR and Western blot. Furthermore, ZFX was found to be significantly up-regulated using Oncomine database analysis. Loss of function assay further indicated knockdown of ZFX had similar effects of miR-144-mediated HCC cell proliferation and invasion. Therefore, miR-144 has been demonstrated to act as a tumour suppressor in HCC cell growth and motility by directly targeting ZFX, which implicates its potential applications in the development of HCC treatment.
Subject(s)
Carcinoma, Hepatocellular/genetics , Kruppel-Like Transcription Factors/biosynthesis , Liver Neoplasms/genetics , MicroRNAs/genetics , Apoptosis/genetics , Carcinoma, Hepatocellular/pathology , Cell Movement/genetics , Cell Proliferation/genetics , Gene Expression Regulation, Neoplastic , Hep G2 Cells , Humans , Kruppel-Like Transcription Factors/genetics , Liver Neoplasms/pathology , MicroRNAs/biosynthesis , Neoplasm Invasiveness/geneticsABSTRACT
Photothermographic material (PTG) is one of the frontier subjects in the field of photographic science during the last decade. In the present paper, the silver-intermediate in development process of PTG material-disilver phthalate (Ag2PA) was prepared by the liquid deposition method, and Ag2PHZ2PA x H2O complex was synthesized successfully by liquid dispersion means using Ag2PA and phthalazine (PHZ) as the reactants. The composition of Ag2PA and Ag2PHZ2PA x H2O was determined by ICP-AES and elemental analysis, and the two silver-intermediates were characterized with XRD, FTIR, TG and DSC. As a result, the silver-intermediates in the development process of PTG material, Ag2PA and Ag2PHZ2PA x H2O, were qualitatively analyzed respectively.
