ABSTRACT
Tricyrtis xianjuensis Li, Chen & Ma 2014 is a rare and endangered species endemic to Zhejiang province, with fewer than 200 individuals in the wild. In our present study, the complete chloroplast genome of T. xianjuensis was assembled by using high-throughput sequencing data, and its genomic features were described and comparative genomic analyses within Liliaceae family were performed. The complete chloroplast genome of T. xianjuensis was 155,748 bp in length, exhibiting a GC content of 37.3%. This genome structure contained two inverted repeats (IRs), as well as a small single-copy (SSC) and a large single-copy (LSC) region. The IR region measured 26,371 bp, while the SSC and LSC regions were 17,729 bp and 85,277 bp in length, respectively. A total of 137 genes were identified, including 85 protein-coding genes, 38 tRNA genes, eight rRNA genes, and six pseudogenes. Phylogenic analysis revealed T. xianjuensis shared a clade with T. formosana Baker 1879 and T. macropoda Miq. 1867, with a support rate of 100%. The assembly and analysis of T. xianjuensis chloroplast genome provided an insight into further studies on the conservation genetics of this endangered species.
ABSTRACT
Impatiens huangyanensis Jin and Ding 2002 is a plant species with very small populations, and it distributes only in Huangyan, Zhejiang Province, China. In this study, the complete chloroplast genome of I. huangyanensis was assembled by using high-throughput Illumina paired-end sequences. Its genomic feature was determined, and comparative genomic analysis of the genus Impatiens was performed. The results revealed that the full-length chloroplast genome of I. huangyanensis was 152,156 bp with a GC content of 36.8%. The chloroplast genome contains a typical quadripartite structure, comprising two copies of inverted repeats (IRs), a small single-copy (SSC) region, and a large single-copy (LSC) region. The sequence lengths of IR, SSC, and LSC were 25,756 bp, 17,662 bp, and 82,982 bp, respectively. The chloroplast genome consisted of 134 genes, including 84 protein-coding genes, 37 transfer RNA genes, eight ribosomal RNA genes, and five pseudogenes. Phylogenic results indicated I. huangyanensis shared a clade with I. davidii Franchet 1883, I. macrovexilla Chen 2000, I. fanjingshanica Chen 1999, and I. piufanensis Hook 1908, with a support rate of 100%. Our study provided insight into further studies on the conservation genetics of I. huangyanensis.
