[Effects of SiRNA-EGFR on the expression of hyaluronidase gene EGFR in human breast cancer cells].

OBJECTIVE: To study the effect of SiRNA-EGFR on the expression of hyaluronidase gene in human breast cancer cells. METHODS: Reverse transcription-polymerse chain reaction was used to detect the changes in the expression of EGFR mRNA in human breast cancer cell lines MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 after transfection by SiRNA-EGFR. RESULTS: After transfection with SiRNA-EGFR, the expression levels of EGFR were significantly inhibited in MDA-MB-231, MDA-MB-435S, ZR-75 and ZR-75-30 cells (P<0.05). CONCLUSION: Transfection by SiRNA-EGFR can inhibit the expression of EGFR mRNA in human breast cancer cells.