ABSTRACT
A produção de bebidas enlatadas segue normas para controle sanitário, porém não é possível estimar as condições higiênicas dessas latas quando chegam ao consumidor. O objetivo do presente trabalho foi analisar externamente latas de cerveja sem selo de proteção, coletadas em prateleiras de supermercado, à temperatura ambiente, quanto à contagem microbiana. As latas foram coletadas, aleatoriamente, em prateleiras de supermercados de Juiz de Fora, MG, à temperatura ambiente e foram submetidas à contagem de micro-organismos aeróbios mesófilos pelo método do esfregaço de suabe. Os resultados demonstraram que as latas testadas sem higienização prévia apresentaram contagem microbiana elevada e que processos de higienização simples, como a lavagem somente com água, podem reduzir consideravelmente a quantidade de micro-organismos na embalagem. A população deve ser esclarecida e instruída a realizar o processo de higienização das latas antes do consumo.
Subject(s)
Beer/microbiology , Food Packaging , Food Hygiene/methodsABSTRACT
INTRODUCTION: In this study, we evaluated the chemical composition of a commercial sample of essential oil from Eucalyptus smithii R.T. Baker and its antifungal activity against Microsporum canis ATCC 32903, Microsporum gypseum ATCC 14683, Trichophyton mentagrophytes ATCC 9533, T. mentagrophytes ATCC 11480, T. mentagrophytes ATCC 11481, and Trichophyton rubrum CCT 5507. METHODS: Morphological changes in these fungi after treatment with the oil were determined by scanning electron microscopy (SEM). The antifungal activity of the oil was determined on the basis of minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values. RESULTS: The compound 1,8-cineole was found to be the predominant component (72.2%) of the essential oil. The MIC values of the oil ranged from 62.5µg·mL-1 to >1,000µg·mL-1, and the MFC values of the oil ranged from 125µg·mL-1 to >1,000µg·mL-1. SEM analysis showed physical damage and morphological alterations in the fungi exposed to this oil. CONCLUSIONS: We demonstrated the potential of Eucalyptus smithii essential oil as a natural therapeutic agent for the treatment of dermatophytosis.
Subject(s)
Antifungal Agents/pharmacology , Eucalyptus/chemistry , Microsporum/drug effects , Oils, Volatile/pharmacology , Plant Extracts/pharmacology , Trichophyton/drug effects , Antifungal Agents/chemistry , Antifungal Agents/isolation & purification , Microbial Sensitivity Tests , Microscopy, Electron, Scanning , Microsporum/classification , Microsporum/ultrastructure , Oils, Volatile/chemistry , Plant Extracts/chemistry , Trichophyton/classification , Trichophyton/ultrastructureABSTRACT
ABSTRACT INTRODUCTION: In this study, we evaluated the chemical composition of a commercial sample of essential oil from Eucalyptus smithii R.T. Baker and its antifungal activity against Microsporum canis ATCC 32903, Microsporum gypseum ATCC 14683, Trichophyton mentagrophytes ATCC 9533, T. mentagrophytes ATCC 11480, T. mentagrophytes ATCC 11481, and Trichophyton rubrum CCT 5507. METHODS: Morphological changes in these fungi after treatment with the oil were determined by scanning electron microscopy (SEM). The antifungal activity of the oil was determined on the basis of minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values. RESULTS: The compound 1,8-cineole was found to be the predominant component (72.2%) of the essential oil. The MIC values of the oil ranged from 62.5μg·mL−1 to >1,000μg·mL−1, and the MFC values of the oil ranged from 125μg·mL−1 to >1,000μg·mL−1. SEM analysis showed physical damage and morphological alterations in the fungi exposed to this oil. CONCLUSIONS: We demonstrated the potential of Eucalyptus smithii essential oil as a natural therapeutic agent for the treatment of dermatophytosis.
Subject(s)
Antifungal Agents/pharmacology , Eucalyptus/chemistry , Microsporum/drug effects , Oils, Volatile/pharmacology , Plant Extracts/pharmacology , Trichophyton/drug effects , Antifungal Agents/chemistry , Antifungal Agents/isolation & purification , Microbial Sensitivity Tests , Microscopy, Electron, Scanning , Microsporum/classification , Microsporum/ultrastructure , Oils, Volatile/chemistry , Plant Extracts/chemistry , Trichophyton/classification , Trichophyton/ultrastructureABSTRACT
This study evaluated the effectiveness of the essential oil of Pimenta pseudocaryophyllus in inhibiting the growth of the main bacteria responsible for bad perspiration odor (Staphylococcus epidermidis, Proteus hauseri, Micrococcus yunnanensis and Corynebacterium xerosis). The chemical profile of the essential oil was evaluated by high-resolution gas chromatography (HR-GC) and four constituents were identified, eugenol being the major component (88.6%). The antimicrobial activity was evaluated by means of the turbidimetric method, using the microdilution assay. The minimum inhibitory concentration (MIC) values of the essential oil ranged from 500 to 1,000 µg mL⻹. Scanning electron microscope (SEM) observations confirmed the physical damage and morphological alteration of the test bacteria treated with the essential oil, reference drugs and eugenol. The findings of the study demonstrated that this essential oil can be used in the formulation of personal care products.
Subject(s)
Anti-Infective Agents/pharmacology , Oils, Volatile/pharmacology , Pimenta , Plant Oils/pharmacology , Anti-Infective Agents/isolation & purification , Gram-Negative Aerobic Bacteria/drug effects , Gram-Negative Aerobic Bacteria/growth & development , Gram-Negative Aerobic Bacteria/ultrastructure , Gram-Positive Bacteria/drug effects , Gram-Positive Bacteria/growth & development , Gram-Positive Bacteria/ultrastructure , Microbial Sensitivity Tests , Microscopy, Electron, Scanning , Oils, Volatile/isolation & purification , Phytotherapy , Pimenta/chemistry , Plant Oils/isolation & purification , Plants, MedicinalABSTRACT
Objective: To confirm previous evidence suggesting an association between autism and low vitamin D serum levels. Methods: This preliminary exploratory study assessed the circulating levels of 25-hydroxyvitamin D (25-OHD) in pediatric patients with autism and in typically developing controls from Juiz de Fora, Brazil. Results: Serum levels of 25-OHD were lower in children with autism (26.48 ± 3.48 ng mL-1) when compared to typically developing subjects (40.52 ± 3.13 ng mL-1) (p < 0.001). Conclusion: Our findings attest to the importance of vitamin supplementation during pregnancy and in the treatment of children with autism, who tend to present low vitamin D consumption rates (AU)
Objetivo: Confirmar evidências prévias indicando uma associação entre autismo e baixos níveis séricos de vitamina D. Métodos: Este estudo preliminar avaliou os níveis circulantes de 25-hidroxivitamina D (25-OHD) em pacientes pediátricos com autismo e em controles apresentando desenvolvimento típico em Juiz de Fora, Brasil. Resultados: Os níveis séricos de 25-OHD foram menores em crianças com autismo (26,48 ± 3,48 ng mL-1) em comparação com indivíduos com desenvolvimento típico (40,52 ± 3,13 ng mL-1) (p < 0,001). Conclusão: Nossos resultados confirmam a importância da suplementação de vitamina durante a gravidez e no tratamento de crianças com autismo, que costumam apresentar um baixo consumo de vitamina D (AU)
Subject(s)
Humans , Male , Female , Child, Preschool , Child , Autistic Disorder/etiology , Vitamin D/analogs & derivatives , Vitamin D Deficiency/complications , Autistic Disorder/blood , Vitamin D/blood , Vitamin D Deficiency/blood , Case-Control Studies , Cross-Sectional StudiesABSTRACT
OBJECTIVE: To confirm previous evidence suggesting an association between autism and low vitamin D serum levels. METHODS: This preliminary exploratory study assessed the circulating levels of 25-hydroxyvitamin D (25-OHD) in pediatric patients with autism and in typically developing controls from Juiz de Fora, Brazil. RESULTS: Serum levels of 25-OHD were lower in children with autism (26.48 ± 3.48 ng mL-1) when compared to typically developing subjects (40.52 ± 3.13 ng mL-1) (p < 0.001). CONCLUSION: Our findings attest to the importance of vitamin supplementation during pregnancy and in the treatment of children with autism, who tend to present low vitamin D consumption rates.
