ABSTRACT
Preserving the mature articular cartilage of joints is a critical focus in the prevention and treatment of osteoarthritis. We determined whether the genetic inactivation of high-temperature requirement A1 (HtrA1) can significantly attenuate the degradation of articular or condylar cartilage. Two types of mouse models of osteoarthritis were used, a spontaneous mutant mouse model [type XI collagen-haploinsufficient (Col11a1+/-) mice] and two post-traumatic mouse models [destabilization of the medial meniscus (DMM) on the knee and a partial discectomy (PDE) on the temporomandibular joint]. Three different groups of mice were generated: i) HtrA1 was genetically deleted from Col11a1+/- mice (HtrA1-/-;Col11a1+/-), ii) HtrA1-deficient mice (HtrA1-/-) were subjected to DMM, and iii) HtrA1-/- mice were subjected to PDE. Knee and temporomandibular joints from the mice were characterized for evidence of cartilage degeneration. The degradation of articular or condylar cartilage was significantly delayed in HtrA1-/-;Col11a1+/- mice and HtrA1-/- mice after DMM or PDE. The amount of collagen type VI was significantly higher in the articular cartilage in HtrA1-/-;Col11a1+/- mice, compared with that in Col11a1+/- mice. The genetic removal of HtrA1 may delay the degradation of articular or condylar cartilage in mice.
Subject(s)
High-Temperature Requirement A Serine Peptidase 1/metabolism , Osteoarthritis/enzymology , Animals , Cartilage, Articular/enzymology , Cartilage, Articular/pathology , Collagen Type X/genetics , Collagen Type X/metabolism , Disease Models, Animal , High-Temperature Requirement A Serine Peptidase 1/genetics , Knee Joint/enzymology , Knee Joint/pathology , Mandibular Condyle/enzymology , Mandibular Condyle/pathology , Mice , Mice, Knockout , Osteoarthritis/genetics , Osteoarthritis/pathologyABSTRACT
O Departamento de Enfermagem do Hospital Virtual Brasileiro tem sido desenvolvido pelo Núcleo de Informática Biomédica (NIB) da Universidade Estadual de Campinas (UNICAMP). É nosso objetivo criar dentro da rede brasileira um repositório de informações baseado na World Wide Web (WWW) sobre Enfermagem e disponibilizar esse material para a comunidade de usuários da Internet e profissionais de enfermagem. Serão utilizadas ferramentas de navegação pela rede e programação HTML (hyper text markup language) para a confecção das páginas WWW. O material reunido está sendo estruturado em áreas de concentração com subdivisões e interconecções.
