ABSTRACT
This study was conducted to evaluate the effect of different concentrations of Limoneno (S)-(-) (50 µM,100 µM and 150 µM) in supplementing the diluter of bulls freezing semen. Thirty-six ejaculated from fourCurraleiro-Pé-Duro bulls were used for cryopreservation. The cryopreserved spermatozoa were submitted to postthawmotility sperm of computer assisted analysis (CASA) to evaluate the characteristics of spermatic kinetics.Observed that The different concentrations of limonene (S) - (-) did not affect the parameters of kinetics spermaticexcept for linearity (LIN). The addition of 150 μM limonene (S) - (-) significantly increased (P <0.05) the LINcompared to the control. The results obtained in the present study allow us to conclude that the supplementationof limonene (S) - (-) in cryopreservation bovine semen diluent did not interfere in kinetics sperm.
Subject(s)
Male , Animals , Cattle , Cattle/embryology , Cryopreservation/methods , Cryopreservation/veterinary , Limonene/administration & dosage , Limonene/analysisABSTRACT
The present study evaluated the effect of melatonin on the degree of embryonic quality during theprocess of in vitro production of embryos. 512 cumulus-oocyte complexes of type I and II were submitted to invitro production of embryos with 0 and 100 µM of melatonin in the maturation medium. There was no differencerelative to the rate of cleavage 36.44% vs 32.93% and total viable embryos 16.44% vs 12.16% (P <0.05).Likewise, there was no difference in the degree of embryonic quality (P <0.05). Therefore, the mediumsupplemented with melatonin was not able to improve the cleavage rate, total viable embryos and quality ofembryos fertilized in vitro.
Subject(s)
Female , Animals , Cattle , Cattle/embryology , Embryo, Mammalian , Melatonin , AntioxidantsABSTRACT
We evaluated the addition of folic acid to the extender TRIS-egg yolk used in cryopreservation, seekingimprovements for the sperm kinetics and mitochondrial activity post-thawing. Were evaluated sevenejaculates/animal from six Santa Inês by Artificial Vagina, with minimum values of motility by 70.0% and vigor3. We evaluated the seminal pool for concentration and morphology. We diluted in Tris-egg yolk extender forcryopreservation, dividing it in control, Group 2 we added 10.000 uM and Group 3 we added 5000 uM of folicacid. We froze in straws(0.25 mL). Was evaluated for motility and vigor, post-thawing by slow term-resistancetest, the times T0, T60, T120 and T180 minutes one week after. For the mitochondrial activity evaluation, wasused cationic lipophilic fluorochrome JC-1. The folic acid effects were analyzed using ANOVA followed byStudent Newman-Keuls test. The groups with folic acid showed significant higher motility. Mitochondrialactivity did not differ between groups.
Subject(s)
Male , Animals , Antioxidants/analysis , Cryopreservation/methods , Cryopreservation/veterinary , Sheep/embryology , Folic Acid/analysis , Folic Acid/analogs & derivativesABSTRACT
This study aims to evaluate the effects of tea of copaíba extract (Copaifera luetzelburgii), in differentconcentrations, added to the Tris-egg yolk extender, the functionality of the plasma membrane sperm ofcryopreserved goats. Were used four goats breeds, adults, male, six of each breed, aged between 1 and 6 years,a pool of ejaculates were formed to analyze. The animals were randomly divided into four experimentaltreatments: TI without adding of tea of copaíba extract (control); TII, added 0,1 mg of tea of copaíba extract;TIII, 0,2 mg; and TIV, 0,5 mg. The samples were frozen and stored, after 30 days were thawed for analysis byhisposmotic test (HOST). Adding tea of copaíba extract showed no significant difference between treatments forHOST, so the use of tea of copaíba extract the seminal dilution was not effective in preserving the functionalityplasma membrane sperm of goats post-thawing.
Subject(s)
Male , Animals , Fabaceae/physiology , Fabaceae/chemistry , Cell Membrane/classification , Cell Membrane/chemistry , Ruminants/embryology , Ruminants/physiology , Cryopreservation/methods , Cryopreservation/veterinaryABSTRACT
The semen cryopreservation process causes damages in spermatozoa characteristics. This study aimedto evaluate the effects of tea of Copaíba extract (Copaifera luetzelburgii), in different concentrations, added tothe Tris-egg yolk extender, in the quality of post-cryopreserved spermatozoa from locally adapted breeds ofgoats. Were used four (4) goats Breeds (Azul, Canindé, Moxotó e Repartido) adults, male, six of each breed,aged between 1 and 6 years, a pool of ejaculates were formed to analyze. The animals were randomly dividedinto four experimental treatments: TI (Control), TII (0,1mg), TIII (0,2mg) and TIV (0,5mg). The samples werefrozen and stored, after 30 days they were thawed for analysis by term-resistance test (TTR). The addition of teaof copaíba extract showed no significant difference between treatments by TTR, therefore the use of tea copaíbaextract in seminal diluting not increment the sperm longevity post-thawing.
Subject(s)
Male , Animals , Cryopreservation/methods , Cryopreservation/veterinary , Fabaceae/chemistry , RuminantsABSTRACT
This work aimed to evaluate the quantitative parameters of fresh and thawed sperm of Canindé goats in theState of Piauí. Ten sperm samples were collected, using artificial vagina, from four goats at reproductive age. Eachejaculate was evaluated for volume, sperm concentration, mass motility, total and progressive motility, percentage ofmotile spermatozoa and vigor, for further dilution in ACP-101. Then, the sperm was filled in 0.25 mL straws,cryopreserved using the TK3000 machine and stored in nitrogen cylinders at -196 °C, for further analysis at SCA. Thesperm parameters were submitted to the T test for comparison between the fresh and thawed groups. The values forfresh sperm relative to total and progressive motility, vigor and sperm viability were higher (P < 0.05) whencompared to thawed, although both fresh and thawed sperm of these animals could be used in programs of artificial insemination (IA).
Subject(s)
Male , Animals , Cryopreservation/methods , Cryopreservation/veterinary , Ruminants/embryology , Ruminants/physiologyABSTRACT
This study evaluated the quality of ejaculated from adapted breeds of goats as the morphologic aspects,functional and thermoresistance. An aliquot of the pool of each breed was used for morphological evaluation bywet preparation, quantifying sperm defects and dividing them into classes. The samples were cryopreservedusing TK3000 machine and evaluated by thermoresistance test by checking motility and sperm vigor every hour.For plasma membrane functionality analysis, was used hiposmotic test. There was no difference (P > 0.05)between the breeds in all sperm defects before and after cryopreservation, as well as for the thermoresistancetimes for motility and sperm vigor (P > 0.05). There was a significant reduction in motility and sperm vigor 120minutes after thawing of the samples. There was no difference (P > 0.05) between the percentage of sperm withfunctional membrane between the evaluated breeds. Therefore, the evaluated breeds have morphology,functionality and similar sperm thermoresistance.
Subject(s)
Male , Animals , Ruminants/anatomy & histology , Ruminants/abnormalities , Ruminants/embryology , Heat Stress Disorders/diagnosis , Heat Stress Disorders/veterinaryABSTRACT
This study aimed to evaluate physically and structurally ejaculates from locally adapted goats in therainy season. Semen pool formed by the four goats of each breed was evaluated for physical, diluted in Tris-eggyolk and frozen. After thawing, it was diluted in Tris and incubated with carboxyfluorescein diacetate andpropidium iodide, to evaluate the integrity of the plasma membrane and JC1 to assess mitochondrial activity.The percentage of cells with damaged membrane and mitochondrial activity in spermatozoa between breed werecompared, every hour for three hours. There was no significant difference (P > 0,05) in relation to thepercentage of cells with damaged plasma membrane, as well as between the incubation times. The Azul breedhad lower mitochondrial activity (P < 0,05). The evaluated semen of all breeds showed no significant damage tothe plasma membrane in the rainy season, but the Azul breed had lower mitochondrial activity in sperm cells.
Subject(s)
Male , Animals , Spermatozoa/classification , Spermatozoa/growth & development , Spermatozoa/chemistry , Ruminants/physiology , Fluorescent Dyes/analysis , Fluorescent Dyes/chemistryABSTRACT
This study evaluate the effect of melatonin supplementation to TRIS-GEMA diluter in plasmatic andmitochondria membrane integrity of semen ram cryopreserved. Was used seven ejaculates of six ram,whichwere pooled in pool, according to the experimental groups: Control; T1 - 1x10-7 mol / L and T2 - 1x10-9 mol / Lmelatonin. Posteriorly the diluting the samples were packaged in straws of 0.25 ml and cryopreserved. Afterthawing at 37°C for 30 seconds, the samples were analyzed for Test thermoresistance slow (TTR) andacrosomal membrane integrity. For statistical analysis we used the Duncan test (α = 0.05) for meancomparison. Treatments 1 and 2 differed statistically control for plasma membrane integrity at times 60, 120and 180min. It was concluded that supplementation 1x10-7mol / L and 1x10-9mol / L of melatonin increased theintegrity of the plasma membrane to 60,120 and 180 minutes.
Subject(s)
Animals , Cell Membrane/classification , Cell Membrane/chemistry , Sheep/embryology , Sheep/physiology , Cryopreservation/veterinary , Melatonin/analysis , Melatonin/adverse effectsABSTRACT
This study to evaluate the effect of melatonin supplementation to TRIS-GEMA diluter in sperm viabilityof semen ram cryopreserved. Was used seven ejaculates of six ram,which were pooled in pool, according to theexperimental groups: T1 - control; T2 - 1x10-7 mol / L and T3 - 1x10-9 mol / L melatonin. Posteriorly thediluting the samples were packaged in straws of 0.25 ml and cryopreserved. After thawing at 37°C for 30seconds, the samples were analyzed for Test thermoresistance slow (TTR) and acrosomal membrane integrity.For statistical analysis we used the Duncan test (α = 0.05) for mean comparison. The results demonstrated thatto the TTR at time 0 min, T2 showed 54.28% motility, and T1obtained lower values in the times 0 and 60 minutesfor force. Supplementation of 1x10-9mol / L of melatonin improved total motility.
Subject(s)
Male , Animals , Cryopreservation/veterinary , Melatonin/analysis , Sheep , Cell SurvivalABSTRACT
This study aimed to evaluate among the seminal characteristics, sperm concentration of Santa Inessheep, aged 22-36 months treated with recombinant bovine somatotropin (rbST). Divided into groups randomly:GI (2mL / 0.9% NaCl), G-II (100mg / rbST) and G-III (125mg / rbST), receiving treatments subcutaneouslyevery 14 days (D0,14,28, 42,56,70). They were measured scrototesticular biometrics. The ejaculates werecollected by artificial vagina, with dummy sheep, and analyzed for volume, vortex, motility, vigor, morphologyand sperm concentration. There was no significant difference (P > 0.05) in PE (30,1cm) and in almost all semenparameters. However, on sperm concentration (sperm / mL) in G-III, there was an increase (787.69 ± 480.72)compared to groups. It was concluded that the dose of 125mg / rbST increases the sperm concentration of ovineSanta Ines.
Subject(s)
Animals , Growth Hormone/adverse effects , Growth Hormone/physiology , Sheep/embryology , Sheep/genetics , Sperm CapacitationABSTRACT
We evaluated the effect of Recombinant Bovine Somatotropin (rbST) seven days before the start of thesynchronization protocol on pregnancy rate in goats. Were used 101 females without defined breed in semiintensivesystem, randomly divided into two groups. Seven days before the start of estrus synchronization, G1 (n= 49) received 125 mg of rbST, IM, and G2 (n = 52) physiological saline, subcutaneously. In D0, the groupsreceived 1 mL of BE, IM, and were synchronized with intravaginal sponges impregnated with 60 mg ofmedroxyprogesterone for 11 days. In D9, they received 300 IU of eCG and 75 µg of PGF2α. On D11, thesponges were removed, we performed IATF 36 and 48 hours later, transcervically. It was used pool of AngloNubians semen. Gestational diagnosis was performed 30 days later. A single dose administration of rbST, sevendays before estrus synchronization, did not increase the pregnancy rate.
Subject(s)
Animals , Goats/embryology , Goats/genetics , Growth Hormone/analysis , Growth Hormone/adverse effects , Insemination, Artificial , OvulationABSTRACT
This study aims to evaluate the fertility rate of sheep semen diluted and cooled to 4ºC in powdercoconut water (ACP-102®) with and without egg yolk in artificial insemination by cervical route in sheep. Tworam lambs and 72 ewe lambs were used, divided into 3 groups: ACP-102® without egg yolk and cooled to 4ºCfor 12 hours, ACP-102® with 2,5% egg yolk also cooled as the first group and a control group with fresh semendiluted in ACP-102® without egg yolk. Pregnancy diagnosis was performed 30 days later. Pregnancy rates weresignificantly different (P < 0.05) between ACP-SGF (58,3%) and ACP-SG4 (20,8%) groups. The ACP-CG4(37,5%) group did not differ in relation to the ACP-SGF. Therefore, the addition of egg yolk to ACP-102®extender favors the viability of sheep semen, cooled to 4ºC for 12 hours and fertility of artificially inseminatedsheep by cervical route.
Subject(s)
Animals , Semen Analysis/veterinary , Egg Yolk/chemistry , Insemination, Artificial/veterinary , Ruminants , Indicator Dilution TechniquesABSTRACT
This study aims to evaluate the fertility rate of sheep semen diluted and cooled to 4ºC in powdercoconut water (ACP-102®) with and without egg yolk in artificial insemination by cervical route in sheep. Tworam lambs and 72 ewe lambs were used, divided into 3 groups: ACP-102® without egg yolk and cooled to 4ºCfor 12 hours, ACP-102® with 2,5% egg yolk also cooled as the first group and a control group with fresh semendiluted in ACP-102® without egg yolk. Pregnancy diagnosis was performed 30 days later. Pregnancy rates weresignificantly different (P < 0.05) between ACP-SGF (58,3%) and ACP-SG4 (20,8%) groups. The ACP-CG4(37,5%) group did not differ in relation to the ACP-SGF. Therefore, the addition of egg yolk to ACP-102®extender favors the viability of sheep semen, cooled to 4ºC for 12 hours and fertility of artificially inseminatedsheep by cervical route.(AU)
Subject(s)
Animals , Semen Analysis/veterinary , Egg Yolk/chemistry , Insemination, Artificial/veterinary , Ruminants , Indicator Dilution TechniquesABSTRACT
This study aimed to evaluate among the seminal characteristics, sperm concentration of Santa Inessheep, aged 22-36 months treated with recombinant bovine somatotropin (rbST). Divided into groups randomly:GI (2mL / 0.9% NaCl), G-II (100mg / rbST) and G-III (125mg / rbST), receiving treatments subcutaneouslyevery 14 days (D0,14,28, 42,56,70). They were measured scrototesticular biometrics. The ejaculates werecollected by artificial vagina, with dummy sheep, and analyzed for volume, vortex, motility, vigor, morphologyand sperm concentration. There was no significant difference (P > 0.05) in PE (30,1cm) and in almost all semenparameters. However, on sperm concentration (sperm / mL) in G-III, there was an increase (787.69 ± 480.72)compared to groups. It was concluded that the dose of 125mg / rbST increases the sperm concentration of ovineSanta Ines.(AU)
Subject(s)
Animals , Growth Hormone/adverse effects , Growth Hormone/physiology , Sheep/embryology , Sheep/genetics , Sperm CapacitationABSTRACT
This study evaluate the effect of melatonin supplementation to TRIS-GEMA diluter in plasmatic andmitochondria membrane integrity of semen ram cryopreserved. Was used seven ejaculates of six ram,whichwere pooled in pool, according to the experimental groups: Control; T1 - 1x10-7 mol / L and T2 - 1x10-9 mol / Lmelatonin. Posteriorly the diluting the samples were packaged in straws of 0.25 ml and cryopreserved. Afterthawing at 37°C for 30 seconds, the samples were analyzed for Test thermoresistance slow (TTR) andacrosomal membrane integrity. For statistical analysis we used the Duncan test (α = 0.05) for meancomparison. Treatments 1 and 2 differed statistically control for plasma membrane integrity at times 60, 120and 180min. It was concluded that supplementation 1x10-7mol / L and 1x10-9mol / L of melatonin increased theintegrity of the plasma membrane to 60,120 and 180 minutes.(AU)
Subject(s)
Animals , Sheep/embryology , Sheep/physiology , Cell Membrane/chemistry , Cell Membrane/classification , Melatonin/adverse effects , Melatonin/analysis , Cryopreservation/veterinaryABSTRACT
This study aimed to evaluate physically and structurally ejaculates from locally adapted goats in therainy season. Semen pool formed by the four goats of each breed was evaluated for physical, diluted in Tris-eggyolk and frozen. After thawing, it was diluted in Tris and incubated with carboxyfluorescein diacetate andpropidium iodide, to evaluate the integrity of the plasma membrane and JC1 to assess mitochondrial activity.The percentage of cells with damaged membrane and mitochondrial activity in spermatozoa between breed werecompared, every hour for three hours. There was no significant difference (P > 0,05) in relation to thepercentage of cells with damaged plasma membrane, as well as between the incubation times. The Azul breedhad lower mitochondrial activity (P < 0,05). The evaluated semen of all breeds showed no significant damage tothe plasma membrane in the rainy season, but the Azul breed had lower mitochondrial activity in sperm cells.(AU)
Subject(s)
Animals , Male , Ruminants/physiology , Spermatozoa/chemistry , Spermatozoa/classification , Spermatozoa/growth & development , Fluorescent Dyes/analysis , Fluorescent Dyes/chemistryABSTRACT
We evaluated the effect of Recombinant Bovine Somatotropin (rbST) seven days before the start of thesynchronization protocol on pregnancy rate in goats. Were used 101 females without defined breed in semiintensivesystem, randomly divided into two groups. Seven days before the start of estrus synchronization, G1 (n= 49) received 125 mg of rbST, IM, and G2 (n = 52) physiological saline, subcutaneously. In D0, the groupsreceived 1 mL of BE, IM, and were synchronized with intravaginal sponges impregnated with 60 mg ofmedroxyprogesterone for 11 days. In D9, they received 300 IU of eCG and 75 µg of PGF2α. On D11, thesponges were removed, we performed IATF 36 and 48 hours later, transcervically. It was used pool of AngloNubians semen. Gestational diagnosis was performed 30 days later. A single dose administration of rbST, sevendays before estrus synchronization, did not increase the pregnancy rate.(AU)
Subject(s)
Animals , Insemination, Artificial , Goats/embryology , Goats/genetics , Growth Hormone/adverse effects , Growth Hormone/analysis , OvulationABSTRACT
This study to evaluate the effect of melatonin supplementation to TRIS-GEMA diluter in sperm viabilityof semen ram cryopreserved. Was used seven ejaculates of six ram,which were pooled in pool, according to theexperimental groups: T1 - control; T2 - 1x10-7 mol / L and T3 - 1x10-9 mol / L melatonin. Posteriorly thediluting the samples were packaged in straws of 0.25 ml and cryopreserved. After thawing at 37°C for 30seconds, the samples were analyzed for Test thermoresistance slow (TTR) and acrosomal membrane integrity.For statistical analysis we used the Duncan test (α = 0.05) for mean comparison. The results demonstrated thatto the TTR at time 0 min, T2 showed 54.28% motility, and T1obtained lower values in the times 0 and 60 minutesfor force. Supplementation of 1x10-9mol / L of melatonin improved total motility.(AU)
Subject(s)
Animals , Male , Sheep , Cryopreservation/veterinary , Cell Survival , Melatonin/analysisABSTRACT
The semen cryopreservation process causes damages in spermatozoa characteristics. This study aimedto evaluate the effects of tea of Copaíba extract (Copaifera luetzelburgii), in different concentrations, added tothe Tris-egg yolk extender, in the quality of post-cryopreserved spermatozoa from locally adapted breeds ofgoats. Were used four (4) goats Breeds (Azul, Canindé, Moxotó e Repartido) adults, male, six of each breed,aged between 1 and 6 years, a pool of ejaculates were formed to analyze. The animals were randomly dividedinto four experimental treatments: TI (Control), TII (0,1mg), TIII (0,2mg) and TIV (0,5mg). The samples werefrozen and stored, after 30 days they were thawed for analysis by term-resistance test (TTR). The addition of teaof copaíba extract showed no significant difference between treatments by TTR, therefore the use of tea copaíbaextract in seminal diluting not increment the sperm longevity post-thawing.(AU)