Economic vulnerability assessment of EU countries to the impact of COVID-19 pandemic with the revised CEV index.

The COVID-19 pandemic had a wide range of detrimental consequences for the global and national economies. It is vital to identify particularly susceptible areas to adopt effective strategies to alleviate the adverse effects of a pandemic. The objective of the paper is to assess the economic vulnerability of EU countries to the COVID-19 pandemic impact using the revised CEV Index. In the study, methods of multivariate statistics were used to analyse the effects of the pandemic. The revised CEVI replaces the 20-dimensional set of features with one aggregate measure, estimated for 27 EU Member States. According to the study, the economic vulnerability of EU countries to the COVID-19 pandemic varies significantly. The most vulnerable countries are in southern Europe, where the tourism sector plays a significant role in GDP composition. Highly susceptible are also Baltic countries: Latvia and Lithuania. The pandemic's harmful impact was the least seen in Germany and Scandinavian countries. The results of this study can be used as a tool for the formulation of policies aimed at overcoming the adverse consequences of economic vulnerability. The CEVI indicates certain areas in the country's economy that make it more fragile. Thus, it can play a significant role in the decision-making process. In the event of a pandemic shock, the CEVI, in combination with other tools, can be an effective instrument for improving the economy's resilience and helping it recover faster.

Covid-19 Economic Vulnerability Index: EU Evidence.

The COVID-19 pandemic outbreak caused many negative effects on both the global and national economies. To implement effective policies to mitigate the negative impact of a pandemic, it is necessary to identify particularly vulnerable areas. The objective of this paper is to rank the EU countries in terms of the level of vulnerability of their economies to the impact of the pandemic. For this purpose, the COVID-19 Economic Vulnerability Index (CEVI) was constructed. It replaces the 15-dimensional set of characteristics of the countries with one aggregate, synthetic indicator estimated for 27 EU member states. In the study multivariate statistical methods, including agglomerative clustering and multi-attribute methods of object assessment were used to analyse the effects of the pandemic. The research shows that EU countries have different levels of economic vulnerability to the impact of the COVID-19 pandemic. The southern European countries (Spain, Croatia, Greece and Italy), where the tourism sector plays an important role in GDP composition, are the most fragile. Germany and the Scandinavian countries proved to be the least sensitive to the negative impact of the pandemic. The CEVI can be an important part of the decision support system. It enables the identification of countries that show greater vulnerability to the economic impact of the COVID-19 pandemic and may help support countries that need help the most. The proposed index also indicates certain areas in the country's economy that make it more vulnerable. The CEVI in combination with other instruments can be a very useful tool to improve the economy's resilience and help it recover faster in the event of a pandemic shock.

Cloning, expression, and purification of a recombinant cold-adapted beta-galactosidase from antarctic bacterium Pseudoalteromonas sp. 22b.

The gram-negative antarctic bacterium Pseudoalteromonas sp. 22b, isolated from the alimentary tract of krill Thyssanoessa macrura, synthesizes an intracellular cold-adapted beta-galactosidase. The gene encoding this beta-galactosidase has been PCR amplified, cloned, expressed in Escherichia coli, purified, and characterized. The enzyme is active as a homotetrameric protein, and each monomer consists of 1028 amino acid residues. The enzyme was purified to homogeneity (50% recovery of activity) by using the fast, two-step procedure, including affinity chromatography on PABTG-Sepharose. Enzymatic properties of the recombinant protein are identical to those of native Pseudoalteromonas sp. 22b beta-galactosidase. The enzyme is cold-adapted and at 10 degrees C retains 20% of maximum activity. The purified enzyme displayed maximum activity close to 40 degrees C and at pH of 6.0-8.0. PNPG was its preferred substrate (58% higher activity than against ONPG). The enzyme was particularly thermolabile, losing all activities within 10 min at 50 degrees C. The hydrolysis of lactose in a milk assay revealed that 90% of milk lactose was hydrolyzed during 6 h at 30 degrees C and during 28 h at 15 degrees C. Because of its attributes, the recombinant Pseudoalteromonas sp. 22b beta-galactosidase could be applied at refrigeration temperatures for production of lactose-reduced dairy products.