ABSTRACT
We studied the expression of activation markers CD25 and CD69 by blood lymphocytes in white mice vaccinated with Brucella abortus 19 BA in antigen-specific tests in vitro. During incubation of blood lymphocytes with brucellosis polysaccharide-protein antigen, a statistically significant increase in the expression of CD25 by B cells and CD69 by T cells was observed; brucellin increased the expression of CD25 by B and T cells. Comparative analysis of the action of antigen preparations B. abortus showed that only brucellin has antigen-specific activity against CD19+CD25+ cells. The used method can be considered as a promising test for evaluation of the effectiveness of brucellosis immunoprophylaxis, which substantiates the need for further research.
Subject(s)
Antigens, Bacterial/administration & dosage , B-Lymphocytes/drug effects , Brucella abortus/immunology , Brucellosis/immunology , T-Lymphocytes/drug effects , Animals , Antigens, Bacterial/immunology , Antigens, CD/genetics , Antigens, CD/immunology , Antigens, Differentiation, T-Lymphocyte/genetics , Antigens, Differentiation, T-Lymphocyte/immunology , B-Lymphocytes/immunology , B-Lymphocytes/microbiology , Brucellosis/diagnosis , Brucellosis/microbiology , Female , Flow Cytometry , Gene Expression , Immunophenotyping , Interleukin-2 Receptor alpha Subunit/genetics , Interleukin-2 Receptor alpha Subunit/immunology , Lectins, C-Type/genetics , Lectins, C-Type/immunology , Lymphocyte Activation/drug effects , Mice , T-Lymphocytes/immunology , T-Lymphocytes/microbiology , VaccinationABSTRACT
The dynamics of LPO marker malondialdehyde formation and peroxidase-destroying activity was studied in homogenized organs of guinea pigs, immunized with thermoextracts from S and L forms Brucella abortus I-206. The L form brucella thermoextract exhibited a lower reactogenicity and adequately activated the antioxidant system, due to which the destructive effects of ROS could be partially neutralized during the vaccinal process.
