ABSTRACT
We sequenced for the first time the complete neurotoxin gene cluster of a nonproteolytic Clostridium botulinum type F. The neurotoxin gene cluster contained a novel gene arrangement that, compared to other C. botulinum neurotoxin gene clusters, lacked the regulatory botR gene and contained an intergenic is element between its orfX2 and orfX3 genes.
Subject(s)
Clostridium botulinum type F/genetics , Clostridium botulinum/genetics , Genes, Bacterial , Multigene Family , DNA Transposable Elements , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Molecular Sequence Data , Open Reading Frames , Sequence Analysis, DNA , Transcription Factors/geneticsABSTRACT
Ninety-two strains of Yersinia pestis recovered over a 21-year period were evaluated for susceptibility to traditional and newer antimicrobial agents. In vitro resistance was noted only against rifampin and imipenem (approximately 20% of strains). The most active compounds (MIC at which 90% of the isolates tested are inhibited) against Y. pestis were cefixime, ceftriaxone, trimethoprim-sulfamethoxazole, and trovafloxacin.
