Subject(s)
Humans , Lung Diseases, Interstitial , Autoimmune Diseases , Biomarkers , Connective TissueABSTRACT
Presentamos el caso de una mujer con diagnóstico de tromboembolia aguda sintomática provocada de riesgo intermedio-altocon posterior inestabilización hemodinámica, convirtiéndose en una embolia de alto riesgo que precisa tratamiento trombolíticourgente. Sin embargo, al presentar una escala BACS de 3 puntos, y tratarse de una paciente de alto riesgo de sangrado, sedecide realizar tratamiento mediante trombectomía percutánea. Así revisamos brevemente la bibliografía disponible actual-mente sobre el tema.(AU)
We present the case of a woman with a diagnosis of acute symptomatic provoked pulmonary embolism of intermediate-highrisk with subsequent hemodynamic inestabilization, turning into a high-risk embolism that requires urgent thrombolytic treat-ment. However, when presenting a 3-point BACS scale, and being a patient at high risk of bleeding, we decided to performtreatment by percutaneous thrombectomy. Thus we briefly review the currently available bibliography on the subject.(AU)
Subject(s)
Humans , Female , Aged, 80 and over , Thrombectomy/methods , Pulmonary Embolism/drug therapy , Pulmonary Embolism/prevention & control , Venous Thrombosis , Metabolic Syndrome , Breast Neoplasms , Therapeutics , Respiratory Tract DiseasesABSTRACT
Se desconoce el significado pronóstico de las anormalidades pulmonares intersticiales (API) observadas en tomografíacomputarizadas realizadas a pacientes sin sospecha de enfermedad pulmonar intersticial. Por ello, presentamos una revisión de la literatura actual para estudiar su evolución y su manejo.(AU)
The prognostic significance of interstitial lung abnormalities (ILAs) observed on computed tomography performed in pa-tients without suspected interstitial lung disease is unknown. Therefore, we present a review of the current literature to study its evolution and management.(AU)
Subject(s)
Humans , Lung Diseases, Interstitial , Prognosis , Tomography, X-Ray Computed , Radiotherapy , Respiratory Tract DiseasesABSTRACT
Las enfermedades pulmonares intersticiales difusas (EPID) se consideran un grupo heterogéneo de patologías que comparten en su mayoría manifestaciones clínicas, radiológicas y funcionales. El diagnóstico de las EPID se basa en la combinación de información clínica y pruebas funcionales, radiológica mediante la tomografía axial computarizada de alta resolución (TCAR) y/o histológica. Sin embargo, el uso de la ecografía pulmonar en la patología intersticial está poco desarrollada, tanto en el screening como en el seguimiento. Realizamos una revisión de la literatura para determinar la utilidad y las limitaciones de la ecografía en dicha patología (AU)
Diffuse interstitial lung diseases (ILD) are considered a heterogeneous group of pathologies that mostly share clinical, radiological and functional manifestations. The diagnosis of ILD is based on the combination of clinical information and functional tests, radiological by high resolution computed tomography (HRCT) and / or histological. However, the use of lung ultrasound in interstitial pathology is poorly developed, both in screening and in follow-up. We conducted a literature review to determine the usefulness and limitations of ultrasound in this pathology (AU)
Subject(s)
Humans , Lung Diseases, Interstitial/diagnostic imaging , Ultrasonography , Follow-Up StudiesABSTRACT
Se describe el caso de una paciente de 50 años que fue ingresada durante la pandemia de SARS-CoV-2 (COVID-19) por neumonía bilateral, atribuida a la infección por dicho virus. Sin embargo, tras realizar pruebas complementarias, se diagnostica una pieza dentaria localizada en fosa nasal como foco infeccioso del proceso. Se concluye con la necesidad de efectuar una correcta anamnesis para valorar otras posibles causas de neumonía durante la pandemia por SARS-CoV-2 (AU)
We describe the case of a 50-year-old patient who was admitted during the SARS-Cov-2 (COVID-19) pandemic for bilateral pneumonia, attributed to infection by this virus. However, after complementary tests, a tooth located in the nostril is diagnosed as an infectious focus of the process. It concludes with the need to carry out a correct anamnesis to assess other possible causes of pneumonia during the SARS-CoV-2 pandemic (AU)
Subject(s)
Humans , Female , Middle Aged , /diagnosis , Pneumonia/diagnosis , Pneumonia/etiology , Tooth Eruption, Ectopic/complications , Nasal Cavity , Diagnosis, DifferentialABSTRACT
Awareness that traditional two-dimensional (2D) in vitro and nonrepresentative animal models may not completely emulate the 3D hierarchical complexity of tissues and organs is on the rise. Therefore, posterior translation into successful clinical application is compromised. To address this dearth, on-chip biomimetic microenvironments powered by microfluidic technologies are being developed to better capture the complexity of in vivo pathophysiology. Here, we describe a "tumor-on-a-chip" model for assessment of precision nanomedicine delivery on which we validate the efficacy of drug-loaded nanoparticles in a gradient fashion. The model validation was performed by viability studies integrated with live imaging to confirm the dose-response effect of cells exposed to the CMCht/PAMAM nanoparticle gradient. This platform also enables the analysis at the gene expression level, where a down-regulation of all the studied genes (MMP-1, Caspase-3, and Ki-67) was observed. This tumor-on-chip model represents an important development in the use of precision nanomedicine toward personalized treatment.
Subject(s)
Colorectal Neoplasms/diagnosis , Lab-On-A-Chip Devices , Nanomedicine/methods , Precision Medicine/methods , Biomimetics , Caspase 3/metabolism , Cell Line, Tumor , Cell Survival , Coculture Techniques , Colorectal Neoplasms/metabolism , Dendrimers/chemistry , Gene Expression Regulation, Neoplastic , HCT116 Cells , Humans , Imaging, Three-Dimensional , Ki-67 Antigen/metabolism , Matrix Metalloproteinase 1/metabolism , Microfluidics , Nanoparticles/chemistryABSTRACT
Microfluidic screening platforms offer new possibilities for performing in vitro cell-based assays with higher throughput and in a setting that has the potential to closely mimic the physiological microenvironment. Integrating functional biomaterials into such platforms is a promising approach to obtain a deeper insight into the interactions occurring at the cell-material interface. The success of such an approach is, however, largely dependent on the ability to miniaturize the biomaterials as well as on the choice of the assay used to study the cell-material interactions. In this work, we developed a microfluidic device, the main component of which is made of a widely used biocompatible polymer, polylactic acid (PLA). This device enabled cell culture under different fluidic regimes, including perfusion and diffusion. Through a combination of photolithography, two-photon polymerization and hot embossing, it was possible to microstructure the surface of the cell culture chamber of the device with highly defined geometrical features. Furthermore, using pyramids with different heights and wall microtopographies as an example, adhesion, morphology and distribution of human MG63 osteosarcoma cells were studied. The results showed that both the height of the topographical features and the microstructural properties of their walls affected cell spreading and distribution. This proof-of-concept study shows that the platform developed here is a useful tool for studying interactions between cells and clinically relevant biomaterials under controlled fluidic regimes.
Subject(s)
Biocompatible Materials , Cell Adhesion , Cell Culture Techniques/instrumentation , Microfluidic Analytical Techniques/instrumentation , Biocompatible Materials/metabolism , Biocompatible Materials/pharmacology , Cell Adhesion/drug effects , Cell Adhesion/physiology , Cell Line, Tumor , Equipment Design , Humans , Microfluidic Analytical Techniques/methodsABSTRACT
An increasing demand exists for biomaterials that are able to actively participate in the process of repair and regeneration of damaged or diseased organs and tissues. Patterning of surfaces of biomaterials with distinct chemical or physical cues is an attractive way to obtain spatial control over their interactions with the biological system. In the current study, micromoulding in capillaries method was used to pattern silicon substrates with bioinert yttria-stabilised zirconia or with bioactive calcium phosphate ceramics, both widely used biomaterials in orthopaedics and dentistry. Micrometer-scale patterns consisted of parallel lines with varying width and spacing. Both ceramics were successfully deposited on the substrate in a pattern defined by the mould. While the yttria-stabilised zirconia pattern was highly homogenous and smooth (Rq = 5.5 nm), the calcium phosphate pattern, consisting of dicalcium phosphate anhydrous before, and of ß-tricalcium phosphate after annealing, exhibited a less homogenous morphology and higher roughness (Rq = 893 nm). Both materials allowed attachment and proliferation of the MG-63 osteosarcoma cell line, independent of the pattern used. While a preferential orientation of cells in the direction of the pattern lines was observed for all patterns, this effect was more pronounced on the lines with a width of up to 20 µm on both yttria-stabilised zirconia and calcium phosphate ceramics, as compared to wider patterns. Furthermore, the cells retained an elongated morphology for a longer period of time on narrow patterns. Micromoulding in capillaries appeared to be a suitable method to pattern both types of ceramics, however further optimisation is needed to improve homogeneity and obtain better control over the chemical phase and crystalline structure of calcium phosphate patterns.
