ABSTRACT
The actions of Hydrocortisone, Peptichemio and of Peptichmio + Hydrocortisone mixture in HeLa cells have been experimented. The results show an increase of cell number in the controls and a lower, but not statistically different growth, in the Hydrocortisone-treated cultures. In the cultures treated with peptichemio the growth is hardly evident, in the ones treated with the Hydrocortisone + Peptichemio mixture, the cell number is decreasing with time. ALP activity in cells treated with drugs is much higher than in the controls ones. The results either on cellular growth and on ALP activity, are compared to the Ara C ones. No significant difference in media ALP activity between drug-treated cultures and controls is evidentiated. The results show in the Peptichemio + Hydrocortisone mixture, that the action of Hydrocortisone is overcome by the Peptichemio one. By the cytochemical technique the enzyme localization is particularly evident in the cytoplasmic inclusions, which are very numerous in the cells treated with Peptichemio.
Subject(s)
Alkaline Phosphatase/metabolism , HeLa Cells/drug effects , Hydrocortisone/pharmacology , Melphalan/analogs & derivatives , Peptichemio/pharmacology , Drug Synergism , HumansABSTRACT
The biochemical and cytochemical effects of cytosine arabinoside (Ara C) and of hydrocortisone on HeLa cell in vitro have been studied. In cultures treated with Ara C, a relationship exists between the cytocidal effects of the drug and an increase in alkaline phosphatase. Although hydrocortisone had no influence on the proliferative rhythm, it induced an increase in alkaline phosphatase. Results obtained with the cytochemical technique were evaluated, particularly in relation to enzyme localization.
