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1.
Sci Signal ; 7(333): ra65, 2014 Jul 08.
Article in English | MEDLINE | ID: mdl-25005229

ABSTRACT

Eukaryotic anion/proton exchangers of the CLC (chloride channel) family mediate anion fluxes across intracellular membranes. The Arabidopsis thaliana anion/proton exchanger AtCLCa is involved in vacuolar accumulation of nitrate. We investigated the role of AtCLCa in leaf guard cells, a specialized plant epidermal cell that controls gas exchange and water loss through pores called stomata. We showed that AtCLCa not only fulfilled the expected role of accumulating anions in the vacuole during stomatal opening but also mediated anion release during stomatal closure in response to the stress hormone abscisic acid (ABA). We found that this dual role resulted from a phosphorylation-dependent change in the activity of AtCLCa. The protein kinase OST1 (also known as SnRK2.6) is a key signaling player and central regulator in guard cells in response to ABA. Phosphorylation of Thr(38) in the amino-terminal cytoplasmic domain of AtCLCa by OST1 increased the outward anion fluxes across the vacuolar membrane, which are essential for stomatal closure. We provide evidence that bidirectional activities of an intracellular CLC exchanger are physiologically relevant and that phosphorylation regulates the transport mode of this exchanger.


Subject(s)
Abscisic Acid/pharmacology , Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Chloride Channels/metabolism , Plant Growth Regulators/pharmacology , Plant Stomata/metabolism , Signal Transduction/drug effects , Abscisic Acid/metabolism , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Chloride Channels/genetics , Phosphorylation/drug effects , Plant Growth Regulators/metabolism , Plant Stomata/genetics , Protein Kinases/genetics , Protein Kinases/metabolism , Signal Transduction/physiology
2.
Plant Mol Biol ; 78(4-5): 431-46, 2012 Mar.
Article in English | MEDLINE | ID: mdl-22294207

ABSTRACT

In mammals, the Voltage-dependent anion channels (VDACs) are predominant proteins of the outer mitochondrial membrane (OMM) where they contribute to the exchange of small metabolites essential for respiration. They were shown to be as well associated with the plasma membrane (PM) and act as redox enzyme or are involved in ATP release for example. In Arabidopsis, we show that four out of six genomic sequences encode AtVDAC proteins. All four AtVDACs are ubiquitously expressed in the plant but each of them displays a specific expression pattern in root cell types. Using two complementary approaches, we demonstrate conclusively that the four expressed AtVDACs are targeted to both mitochondria and plasma membrane but in differential abundance, AtVDAC3 being the most abundant in PM, and conversely, AtVDAC4 almost exclusively associated with mitochondria. These are the first plant proteins to be shown to reside in both these two membranes. To investigate a putative function of AtVDACs, we analyzed T-DNA insertion lines in each of the corresponding genes. Knock-out mutants for AtVDAC1, AtVDAC2 and AtVDAC4 present slow growth, reduced fertility and yellow spots in leaves when atvdac3 does not show any visible difference compared to wildtype plants. Analyses of atvdac1 and atvdac4 reveal that yellow areas correspond to necrosis and the mitochondria are swollen in these two mutants. All these results suggest that, in spite of a localization in plasma membrane for three of them, AtVDAC1, AtVDAC2 and AtVDAC4 have a main function in mitochondria.


Subject(s)
Arabidopsis Proteins/metabolism , Gene Expression Regulation, Plant , Mitochondria/metabolism , Voltage-Dependent Anion Channels/metabolism , Arabidopsis/genetics , Arabidopsis/growth & development , Arabidopsis Proteins/genetics , Cell Membrane/metabolism , DNA, Bacterial , Gene Knockout Techniques , Mitochondria/genetics , Necrosis , Plant Leaves/genetics , Plant Leaves/metabolism , Voltage-Dependent Anion Channels/genetics
3.
Annu Rev Plant Biol ; 62: 25-51, 2011.
Article in English | MEDLINE | ID: mdl-21275645

ABSTRACT

Anion channels/transporters are key to a wide spectrum of physiological functions in plants, such as osmoregulation, cell signaling, plant nutrition and compartmentalization of metabolites, and metal tolerance. The recent identification of gene families encoding some of these transport systems opened the way for gene expression studies, structure-function analyses of the corresponding proteins, and functional genomics approaches toward further understanding of their integrated roles in planta. This review, based on a few selected examples, illustrates that the members of a given gene family exhibit a diversity of substrate specificity, regulation, and intracellular localization, and are involved in a wide range of physiological functions. It also shows that post-translational modifications of transport proteins play a key role in the regulation of anion transport activity. Key questions arising from the increasing complexity of networks controlling anion transport in plant cells (the existence of redundancy, cross talk, and coordination between various pathways and compartments) are also addressed.


Subject(s)
Antiporters/physiology , Arabidopsis Proteins/physiology , Arabidopsis/metabolism , Antiporters/genetics , Antiporters/metabolism , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Biological Transport/physiology , Cell Membrane/chemistry , Cell Membrane/metabolism , Gene Expression Regulation, Plant , Models, Biological , Multigene Family/physiology , Nitrates/metabolism , Phosphorylation , Protein Processing, Post-Translational , Substrate Specificity
4.
Plant Signal Behav ; 5(11): 1347-52, 2010 Nov.
Article in English | MEDLINE | ID: mdl-21051946

ABSTRACT

Plant genomes code for channels involved in the transport of cations, anions and uncharged molecules through membranes. Although the molecular identity of channels for cations and uncharged molecules has progressed rapidly in the recent years, the molecular identity of anion channels has lagged behind. Electrophysiological studies have identified S-type (slow) and R-type (rapid) anion channels. In this brief review, we summarize the proposed functions of the R-type anion channels which, like the S-type, were first characterized by electrophysiology over 20 years ago, but unlike the S-type, have still yet to be cloned. We show that the R-type channel can play multiple roles.


Subject(s)
Calcium Channels, R-Type/physiology , Gene Expression Regulation, Plant/physiology , Plant Proteins/physiology , Plants/metabolism , Calcium Signaling , Ion Channel Gating/physiology
6.
Plant J ; 64(4): 563-76, 2010 Nov.
Article in English | MEDLINE | ID: mdl-20822503

ABSTRACT

In plant cells, anion channels and transporters are essential for key functions such as nutrition, resistance to biotic or abiotic stresses, and ion homeostasis. In Arabidopsis, members of the chloride channel (CLC) family located in intracellular organelles have been shown to be required for nitrate homeostasis or pH adjustment, and previous results indicated that AtCLCc is involved in nitrate accumulation. We investigated new physiological functions of this CLC member in Arabidopsis. Here we report that AtCLCc is strongly expressed in guard cells and pollen and more weakly in roots. Use of an AtCLCc:GFP fusion revealed localization to the tonoplast. Disruption of the AtCLCc gene by a T-DNA insertion in four independent lines affected physiological responses that are directly related to the movement of chloride across the tonoplast membrane. Opening of clcc stomata was reduced in response to light, and ABA treatment failed to induce their closure, whereas application of KNO3 but not KCl restored stomatal opening. clcc mutant plants were hypersensitive to NaCl treatment when grown on soil, and to NaCl and KCl in vitro, confirming the chloride dependence of the phenotype. These phenotypes were associated with modifications of chloride content in both guard cells and roots. These data demonstrate that AtCLCc is essential for stomatal movement and salt tolerance by regulating chloride homeostasis.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Chloride Channels/metabolism , Plant Stomata/physiology , Salt Tolerance , Abscisic Acid/metabolism , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Chloride Channels/genetics , Gene Expression Regulation, Plant , Light , Plant Epidermis/metabolism , Plant Roots/metabolism , Pollen/metabolism , Salinity , Sodium Chloride , Up-Regulation
7.
Plant J ; 63(5): 861-9, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20598093

ABSTRACT

Nitrate, the major nitrogen source for plants, can be accumulated in the vacuole. Its transport across the vacuolar membrane is mediated by AtCLCa, an antiporter of the chloride channel (CLC) protein family. In contrast to other CLC family members, AtCLCa transports nitrate coupled to protons. Recently, the different behaviour towards nitrate of CLC proteins has been linked to the presence of a serine or proline in the selectivity filter motif GXGIP. By monitoring AtCLCa activity in its native environment, we show that if proline 160 in AtCLCa is changed to a serine (AtCLCa(P160S) ), the transporter loses its nitrate selectivity, but the anion proton exchange mechanism is unaffected. We also performed in vivo analyses in yeast and Arabidopsis. In contrast to native AtCLCa, expression of AtCLCa(P160S) does not complement either the ΔScCLC yeast mutant grown on nitrate or the nitrate under-accumulation phenotype of clca knockout plants. Our results confirm the significance of this amino acid in the conserved selectivity filter of CLC proteins and highlight the importance of the proline in AtCLCa for nitrate metabolism in Arabidopsis.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Chloride Channels/metabolism , Nitrates/metabolism , Proline/metabolism , Amino Acid Motifs/genetics , Amino Acid Sequence , Amino Acid Substitution , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Chloride Channels/genetics , Genetic Complementation Test , Ion Transport , Membrane Potentials , Molecular Sequence Data , Mutation , Patch-Clamp Techniques , Proline/genetics , Protoplasts/metabolism , Protoplasts/physiology , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism , Sequence Homology, Amino Acid , Transfection
8.
Plant Physiol ; 152(4): 1986-99, 2010 Apr.
Article in English | MEDLINE | ID: mdl-20181755

ABSTRACT

Manganese (Mn) is an essential element, acting as cofactor in numerous enzymes. In particular, a Mn cluster is indispensable for the function of the oxygen-evolving complex of photosystem II. Metal transporters of the Natural Resistance-Associated Macrophage Protein (NRAMP) family have the ability to transport both iron and Mn. AtNRAMP3 and AtNRAMP4 are required for iron mobilization in germinating seeds. The results reported here show that, in adult Arabidopsis (Arabidopsis thaliana) plants, AtNRAMP3 and AtNRAMP4 have an important role in Mn homeostasis. Vacuolar Mn accumulation in mesophyll cells of rosette leaves of adult nramp3nramp4 double mutant plants was dramatically increased when compared with the wild type. This suggests that a considerable proportion of the cellular Mn pool passes through the vacuole and is retrieved in an AtNRAMP3/AtNRAMP4-dependent manner. The impaired Mn release from mesophyll vacuoles of nramp3nramp4 double mutant plants is associated with reduced growth under Mn deficiency. However, leaf AtNRAMP3 and AtNRAMP4 protein levels are unaffected by Mn supply. Under Mn deficiency, nramp3nramp4 plants contain less functional photosystem II than the wild type. These data are consistent with a shortage of Mn to produce functional photosystem II, whereas mitochondrial Mn-dependent superoxide dismutase activity is maintained under Mn deficiency in both genotypes. The results presented here suggest an important role for AtNRAMP3/AtNRAMP4-dependent Mn transit through the vacuole prior to the import into chloroplasts of mesophyll cells.


Subject(s)
Arabidopsis Proteins/physiology , Arabidopsis/physiology , Cation Transport Proteins/physiology , Manganese/metabolism , Photosynthesis , Vacuoles/metabolism , Arabidopsis/enzymology , Arabidopsis/growth & development , Arabidopsis/metabolism , Biological Transport , Plant Leaves/metabolism , Protoplasts/metabolism , Superoxide Dismutase/metabolism
9.
J Biol Chem ; 284(39): 26526-32, 2009 Sep 25.
Article in English | MEDLINE | ID: mdl-19636075

ABSTRACT

Nitrate, one of the major nitrogen sources for plants, is stored in the vacuole. Nitrate accumulation within the vacuole is primarily mediated by the NO(3)(-)/H(+) exchanger AtCLCa, which belongs to the chloride channel (CLC) family. Crystallography analysis of hCLC5 suggested that the C-terminal domain, composed by two cystathionine beta-synthetase motifs in all eukaryotic members of the CLC family is able to interact with ATP. However, interaction of nucleotides with a functional CLC protein has not been unambiguously demonstrated. Here we show that ATP reversibly inhibits AtCLCa by interacting with the C-terminal domain. Applying the patch clamp technique to isolated Arabidopsis thaliana vacuoles, we demonstrate that ATP reduces AtCLCa activity with a maximum inhibition of 60%. ATP inhibition of nitrate influx into the vacuole at cytosolic physiological nitrate concentrations suggests that ATP modulation is physiologically relevant. ADP and AMP do not decrease the AtCLCa transport activity; nonetheless, AMP (but not ADP) competes with ATP, preventing inhibition. A molecular model of the C terminus of AtCLCa was built by homology to hCLC5 C terminus. The model predicted the effects of mutations of the ATP binding site on the interaction energy between ATP and AtCLCa that were further confirmed by functional expression of site-directed mutated AtCLCa.


Subject(s)
Adenosine Triphosphate/metabolism , Arabidopsis Proteins/metabolism , Chloride Channels/metabolism , Adenosine Diphosphate/pharmacology , Adenosine Monophosphate/pharmacology , Adenosine Triphosphate/pharmacology , Algorithms , Amino Acid Sequence , Arabidopsis Proteins/chemistry , Arabidopsis Proteins/genetics , Binding Sites/genetics , Chloride Channels/chemistry , Chloride Channels/genetics , Dose-Response Relationship, Drug , Ion Transport/drug effects , Kinetics , Membrane Potentials/drug effects , Models, Molecular , Molecular Sequence Data , Nitrates/metabolism , Protein Binding , Protein Structure, Tertiary , Protoplasts/cytology , Protoplasts/metabolism , Sequence Homology, Amino Acid , Static Electricity , Vacuoles/drug effects , Vacuoles/metabolism
10.
New Phytol ; 183(1): 88-94, 2009.
Article in English | MEDLINE | ID: mdl-19402883

ABSTRACT

* In plants, the knowledge of the molecular identity and functions of anion channels are still very limited, and are almost restricted to the large ChLoride Channel (CLC) family. In Arabidopsis thaliana, some genetic evidence has suggested a role for certain AtCLC protein members in the control of plant nitrate levels. In this context, AtClCa has been demonstrated to be involved in nitrate transport into the vacuole, thereby participating in cell nitrate homeostasis. * In this study, analyses of T-DNA insertion mutants within the AtClCa and AtClCe genes revealed common phenotypic traits: a lower endogenous nitrate content; a higher nitrite content; a reduced nitrate influx into the root; and a decreased expression of several genes encoding nitrate transporters. * This set of nitrate-related phenotypes, displayed by clca and clce mutant plants, showed interconnecting roles of AtClCa and AtClCe in nitrate homeostasis involving two different endocellular membranes. * In addition, it revealed cross-talk between two nitrate transporter families participating in nitrate assimilation pathways. The contribution to nitrate homeostasis at the cellular level of members of these different families is discussed.


Subject(s)
Anion Transport Proteins/metabolism , Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Chloride Channels/metabolism , Genes, Plant , Ion Transport/physiology , Nitrates/metabolism , Nitrites/metabolism , Anion Transport Proteins/genetics , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Chloride Channels/genetics , DNA, Bacterial , Intracellular Membranes/metabolism , Metabolic Networks and Pathways , Mutation , Nitrate Transporters , Phenotype , Receptor Cross-Talk , Signal Transduction , Vacuoles/metabolism
11.
Philos Trans R Soc Lond B Biol Sci ; 364(1514): 195-201, 2009 Jan 27.
Article in English | MEDLINE | ID: mdl-18957376

ABSTRACT

Plants need nitrate for growth and store the major part of it in the central vacuole of cells from root and shoot tissues. Based on few studies on the two model plants Arabidopsis thaliana and rice, members of the large ChLoride Channel (CLC) family have been proposed to encode anion channels/transporters involved in nitrate homeostasis. Proteins from the Arabidopsis CLC family (AtClC, comprising seven members) are present in various membrane compartments including the vacuolar membrane (AtClCa), Golgi vesicles (AtClCd and AtClCf) or chloroplast membranes (AtClCe). Through a combination of electrophysiological and genetic approaches, AtClCa was shown to function as a 2NO3-/1H+ exchanger that is able to accumulate specifically nitrate into the vacuole, in agreement with the main phenotypic trait of knockout mutant plants that accumulate 50 per cent less nitrate than their wild-type counterparts. The set-up of a functional complementation assay relying on transient expression of AtClCa cDNA in the mutant background opens the way for studies on structure-function relationships of the AtClCa nitrate transporter. Such studies will reveal whether important structural determinants identified in bacterial or mammalian CLCs are also crucial for AtClCa transport activity and regulation.


Subject(s)
Anions/metabolism , Chloride Channels/metabolism , Plant Cells , Plants/metabolism , Plant Proteins/metabolism
12.
New Phytol ; 181(3): 637-50, 2009.
Article in English | MEDLINE | ID: mdl-19054339

ABSTRACT

The ability of metal hyperaccumulating plants to tolerate and accumulate heavy metals results from adaptations of metal homeostasis. NRAMP metal transporters were found to be highly expressed in some hyperaccumulating plant species. Here, we identified TcNRAMP3 and TcNRAMP4, the closest homologues to AtNRAMP3 and AtNRAMP4 in Thlaspi caerulescens and characterized them by expression analysis, confocal imaging and heterologous expression in yeast and Arabidopsis thaliana. TcNRAMP3 and TcNRAMP4 are expressed at higher levels than their A. thaliana homologues. When expressed in yeast TcNRAMP3 and TcNRAMP4 transport the same metals as their respective A. thaliana orthologues: iron (Fe), manganese (Mn) and cadmium (Cd) but not zinc (Zn) for NRAMP3; Fe, Mn, Cd and Zn for NRAMP4. They also localize at the vacuolar membrane in A. thaliana protoplasts. Inactivation of AtNRAMP3 and AtNRAMP4 in A. thaliana results in strong Cd and Zn hypersensitivity, which is fully rescued by TcNRAMP3 or TcNRAMP4 expression. However, metal tolerance conferred by TcNRAMP expression in nramp3nramp4 mutant does not exceed that of wild-type A. thaliana. Our data indicate that the difference between TcNRAMP3 and TcNRAMP4 and their A. thaliana orthologues does not lie in a different protein function, but probably resides in a different expression level or expression pattern.


Subject(s)
Metals/metabolism , Plant Proteins/metabolism , Thlaspi/metabolism , Arabidopsis/drug effects , Arabidopsis/metabolism , Biological Transport/drug effects , Cadmium/toxicity , Cloning, Molecular , DNA, Complementary/genetics , DNA, Plant/metabolism , Gene Expression Regulation, Plant/drug effects , Genetic Complementation Test , Genome, Plant/genetics , Green Fluorescent Proteins/metabolism , Intracellular Membranes/drug effects , Intracellular Membranes/metabolism , Molecular Sequence Data , Mutation/genetics , Plant Proteins/genetics , Recombinant Fusion Proteins/metabolism , Saccharomyces cerevisiae/cytology , Saccharomyces cerevisiae/drug effects , Thlaspi/drug effects , Thlaspi/genetics , Vacuoles/drug effects , Vacuoles/metabolism , Zinc/toxicity
13.
Funct Plant Biol ; 36(9): 832-843, 2009 Sep.
Article in English | MEDLINE | ID: mdl-32688693

ABSTRACT

Plants are constantly exposed to environmental biotic and abiotic stresses. Plants cells perceive these factors and trigger early responses followed by delayed and complex adaptation processes. Using cell suspensions of Arabidopsis thaliana (L.) as a cellular model, we investigated the role of plasma membrane anion channels in Reactive Oxygen Species (ROS) production and in cell death which occurs during non-host pathogen infection. Protoplasts derived from Arabidopsis suspension cells display two anion currents with characteristics very similar to those of the slow nitrate-permeable (S-type) and rapid sulfate-permeable (R-type) channels previously characterised in hypocotyl cells and other cell types. Using seven inhibitors, we showed that the R-type channel and ROS formation in cell cultures present similar pharmacological profiles. The efficiency of anion channel blockers to inhibit ROS production was independent of the nature of the triggering signal (osmotic stress or general elicitors of plant defence), indicating that the R-type channel represents a crossroad in the signalling pathways leading to ROS production. In a second step, we show that treatment with R-type channel blockers accelerates cell death triggered by the non-specific plant pathogen Xanthomonas campestris. Finally, we discuss the hypothesis that the R-type channel is involved in innate immune response allowing cell defence via antibacterial ROS production.

14.
Curr Biol ; 18(10): 730-734, 2008 May 20.
Article in English | MEDLINE | ID: mdl-18485707

ABSTRACT

In bacterial and animal systems, mechanosensitive (MS) ion channels are thought to mediate the perception of pressure, touch, and sound [1-3]. Although plants respond to a wide variety of mechanical stimuli, and although many mechanosensitive channel activities have been characterized in plant membranes by the patch-clamp method, the molecular nature of mechanoperception in plant systems has remained elusive [4]. Likely candidates are relatives of MscS (Mechanosensitive channel of small conductance), a well-characterized MS channel that serves to protect E. coli from osmotic shock [5]. Ten MscS-Like (MSL) proteins are found in the genome of the model flowering plant Arabidopsis thaliana[4, 6, 7]. MSL2 and MSL3, along with MSC1, a MscS family member from green algae, are implicated in the control of organelle morphology [8, 9]. Here, we characterize MSL9 and MSL10, two MSL proteins found in the plasma membrane of root cells. We use a combined genetic and electrophysiological approach to show that MSL9 and MSL10, along with three other members of the MSL family, are required for MS channel activities detected in protoplasts derived from root cells. This is the first molecular identification and characterization of MS channels in plant membranes.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/metabolism , Mechanotransduction, Cellular , Membrane Proteins/metabolism , Plant Roots/metabolism , Amino Acid Sequence , Cell Membrane/metabolism , Molecular Sequence Data , Protoplasts/metabolism
15.
Plant Signal Behav ; 3(9): 726-9, 2008 Sep.
Article in English | MEDLINE | ID: mdl-19704841

ABSTRACT

Plant cells, like those of animals and bacteria, are able to sense physical deformation of the plasma membrane. Mechanosensitive (MS) channels are proteins that transduce mechanical force into ion flux, providing a mechanism for the perception of mechanical stimuli such as sound, touch and osmotic pressure. We recently identified AtMSL9 and AtMSL10, two mechanosensitive channels in Arabidopsis thaliana, as molecular candidates for mechanosensing in higher plants.1 AtMSL9 and AtMSL10 are members of a family of proteins in Arabidopsis that are related to the bacterial MS channel MscS, termed MscS-Like (or MSL).2 MscS (Mechanosensitive channel of Small conductance) is one of the best-characterized MS channels, first identified as an electrophysiological activity in the plasma membrane (PM) of giant E. coli spheroplasts.3,4 Activation of MscS is voltage-independent, but responds directly to tension applied to the membrane and does not require other cellular proteins for this regulation.5,6 MscS family members are widely distributed throughout bacterial and archaeal genomes, are present in all plant genomes yet examined, and are found in selected fungal genomes.2,7,8 MscS homolgues have not yet been identified in animals.

16.
J Exp Bot ; 58(12): 3385-93, 2007.
Article in English | MEDLINE | ID: mdl-17872921

ABSTRACT

Though numerous pieces of evidence point to major physiological roles for anion channels in plants, progress in the understanding of their biological functions is limited by the small number of genes identified so far. Seven chloride channel (CLC) members could be identified in the Arabidopsis genome, amongst which AtCLCe and AtCLCf are both more closely related to bacterial CLCs than the other plant CLCs. It is shown here that AtCLCe is targeted to the thylakoid membranes in chloroplasts and, in agreement with this subcellular localization, that the clce mutants display a phenotype related to photosynthesis activity. The AtCLCf protein is localized in Golgi membranes and functionally complements the yeast gef1 mutant disrupted in the single CLC gene encoding a Golgi-associated protein.


Subject(s)
Arabidopsis/metabolism , Chloride Channels/metabolism , Golgi Apparatus/metabolism , Thylakoids/metabolism , Arabidopsis/genetics , Arabidopsis/physiology , Blotting, Western , Electrophoresis, Polyacrylamide Gel , Genome, Plant , Photosynthesis , Spectrometry, Fluorescence
17.
Mol Cell Proteomics ; 6(11): 1980-96, 2007 Nov.
Article in English | MEDLINE | ID: mdl-17644812

ABSTRACT

The proteomics of plasma membrane has brought to date only scarce and partial information on the actual protein repertoire. In this work, the plant plasma membrane proteome of Arabidopsis thaliana was investigated. A highly purified plasma membrane fraction was washed by NaCl and Na2CO3 salts, and the insoluble fractions were further analyzed by nano-LC-MS/MS. With 446 proteins identified, we hereby describe the largest plasma membrane proteome diversity reported so far. Half of the proteins were predicted to display transmembrane domains and/or to be anchored to the membrane, validating a posteriori the pertinence of the approach. A fine analysis highlighted two main specific and novel features. First, the main functional category is represented by a majority of as yet unreported signaling proteins, including 11% receptor-like kinases. Second, 16% of the identified proteins are predicted to be lipid-modified, specifically involving double lipid linkage through N-terminal myristoylation, S-palmitoylation, C-terminal prenylation, or glycosylphosphatidylinositol anchors. Thus, our approach led for the first time to the identification of a large number of peripheral proteins as part of the plasma membrane and allowed the functionality of the plasma membrane in the cell context to be reconsidered.


Subject(s)
Arabidopsis Proteins/analysis , Arabidopsis/chemistry , Lipoylation , Membrane Proteins/analysis , Proteome/analysis , Cell Membrane/chemistry , Chromatography, Liquid , Lipids/analysis , Mass Spectrometry , Phosphotransferases/analysis , Proteomics , Salts/chemistry
18.
FEBS Lett ; 581(12): 2367-74, 2007 May 25.
Article in English | MEDLINE | ID: mdl-17434490

ABSTRACT

Anion channels/transporters appear as key players in signaling pathways leading to the adaptation of plant cells to abiotic and biotic environmental stresses, in the control of metabolism and in the maintenance of electrochemical gradients. Focusing on the most recent advances, this review aims at providing a description of the role of these channels in various physiological functions such as control of stomatal movements, plant-pathogen interaction, xylem loading, compartmentalization of metabolites and coupling with proton gradients. These functions have been demonstrated by a combination of electrophysiology, pharmacology and genetics approaches, the key issue being to identify the corresponding proteins and genes.


Subject(s)
Anion Transport Proteins/metabolism , Ion Channels/metabolism , Plants/metabolism , Anions/metabolism , Cell Membrane/metabolism , Malates/metabolism , Models, Biological , Nitrate Transporters , Plant Cells , Proton Pumps/metabolism , Signal Transduction , Xylem/metabolism
19.
Proteomics ; 7(5): 750-4, 2007 Mar.
Article in English | MEDLINE | ID: mdl-17285564

ABSTRACT

An important goal for proteomic studies is the global comparison of proteomes from different genotypes, tissues, or physiological conditions. This has so far been mostly achieved by densitometric comparison of spot intensities after protein separation by 2-DE. However, the physicochemical properties of membrane proteins preclude the use of 2-DE. Here, we describe the use of in vivo labeling by the stable isotope 15N as an alternative approach for comparative membrane proteomic studies in plant cells. We confirm that 15N-metabolic labeling of proteins is possible and efficient in Arabidopsis suspension cells. Quantification of 14N versus 15N MS signals reflects the relative abundance of 14N and 15N proteins in the sample analyzed. We describe the use of 15N-metabolic labeling to perform a partial comparative analysis of Arabidopsis cells following cadmium exposure. By focusing our attention on plasma membrane proteins, we were able to confidently identify proteins showing up to 5-fold regulation compared to unexposed cells. This study provides a proof of principle that 15N-metabolic labeling is a useful technique for comparative membrane proteome studies.


Subject(s)
Arabidopsis/metabolism , Cell Membrane/metabolism , Nitrogen/metabolism , Proteomics/methods , Cells, Cultured , Nitrogen Isotopes
20.
Plant Mol Biol ; 63(4): 491-503, 2007 Mar.
Article in English | MEDLINE | ID: mdl-17103012

ABSTRACT

In Arabidopsis cell suspension, hyperosmotic stresses (mannitol and NaCl) were previously shown to activate nine sucrose non-fermenting 1 related protein kinases 2 (SnRK2s) whereas only five of them were also activated by abscisic acid (ABA) treatment. Here, the possible activation by phosphorylation/ dephosphorylation of each kinase was investigated by studying their phosphorylation state after osmotic stress, using the Pro-Q Diamond, a specific dye for phosphoproteins. All the activated kinases were phosphorylated after osmotic stress but the induced phosphorylation changes were clearly different depending on the kinase. In addition, the increase of the global phosphorylation level induced by ABA application was lower, suggesting that different mechanisms may be involved in SnRK2 activation by hyperosmolarity and ABA. On the other hand, SnRK2 kinases remain activated by hyperosmotic stress in ABA-deficient and ABA-insensitive mutants, indicating that SnRK2 osmotic activation is independent of ABA. Moreover, using a mutant form of SnRK2s, a specific serine in the activation loop was shown to be phosphorylated after stress treatments and essential for activity and/or activation. Finally, SnRK2 activity was sensitive to staurosporine, whereas SnRK2 activation by hyperosmolarity or ABA was not, indicating that SnRK2 activation by phosphorylation is mediated by an upstream staurosporine-insensitive kinase, in both signalling pathways. All together, these results indicate that different phosphorylation mechanisms and at least three signalling pathways are involved in the activation of SnRK2 proteins in response to osmotic stress and ABA.


Subject(s)
Abscisic Acid/metabolism , Arabidopsis Proteins/metabolism , Phosphoproteins/metabolism , Protein Serine-Threonine Kinases/metabolism , Amino Acid Sequence , Arabidopsis/enzymology , Arabidopsis/genetics , Arabidopsis Proteins/genetics , Base Sequence , DNA Primers , Molecular Sequence Data , Mutagenesis, Site-Directed , Osmolar Concentration , Phosphorylation , Protein Serine-Threonine Kinases/genetics , Protoplasts/enzymology , Recombinant Proteins/metabolism , Serine
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