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1.
Front Cell Infect Microbiol ; 13: 1125482, 2023.
Article in English | MEDLINE | ID: mdl-36875521

ABSTRACT

α-helical coiled-coils are ubiquitous protein structures in all living organisms. For decades, modified coiled-coils sequences have been used in biotechnology, vaccine development, and biochemical research to induce protein oligomerization, and form self-assembled protein scaffolds. A prominent model for the versatility of coiled-coil sequences is a peptide derived from the yeast transcription factor, GCN4. In this work, we show that its trimeric variant, GCN4-pII, binds bacterial lipopolysaccharides (LPS) from different bacterial species with picomolar affinity. LPS molecules are highly immunogenic, toxic glycolipids that comprise the outer leaflet of the outer membrane of Gram-negative bacteria. Using scattering techniques and electron microscopy, we show how GCN4-pII breaks down LPS micelles in solution. Our findings suggest that the GCN4-pII peptide and derivatives thereof could be used for novel LPS detection and removal solutions with high relevance to the production and quality control of biopharmaceuticals and other biomedical products, where even minuscule amounts of residual LPS can be lethal.


Subject(s)
Glycolipids , Lipopolysaccharides , Protein Domains , Saccharomyces cerevisiae
2.
Biophys J ; 122(11): 2147-2161, 2023 06 06.
Article in English | MEDLINE | ID: mdl-36523159

ABSTRACT

Gram-negative bacteria are equipped with a cell wall that contains a complex matrix of lipids, proteins, and glycans, which form a rigid layer protecting bacteria from the environment. Major components of this outer membrane are the high-molecular weight and amphiphilic lipopolysaccharides (LPSs). They form the extracellular part of a heterobilayer with phospholipids. Understanding LPS properties within the outer membrane is therefore important to develop new antimicrobial strategies. Model systems, such as giant unilamellar vesicles (GUVs), provide a suitable platform for exploring membrane properties and interactions. However, LPS molecules contain large polysaccharide parts that confer high water solubility, which makes LPS incorporation in artificial membranes difficult; this hindrance is exacerbated for LPS with long polysaccharide chains, i.e., the smooth LPS. Here, a novel emulsification step of the inverted emulsion method is introduced to incorporate LPS in the outer or the inner leaflet of GUVs, exclusively. We developed an approach to determine the LPS content on individual GUVs and quantify membrane asymmetry. The asymmetric membranes with outer leaflet LPS show incorporations of 1-16 mol % smooth LPS (corresponding to 16-79 wt %), while vesicles with inner leaflet LPS reach coverages of 2-7 mol % smooth LPS (28-60 wt %). Diffusion coefficient measurements in the obtained GUVs showed that increasing LPS concentrations in the membranes resulted in decreased diffusivity.


Subject(s)
Biomimetics , Lipopolysaccharides , Lipopolysaccharides/metabolism , Phospholipids/metabolism , Membranes, Artificial , Unilamellar Liposomes/metabolism , Bacteria/metabolism , Cell Membrane/metabolism , Bacterial Outer Membrane Proteins/metabolism
3.
EMBO Mol Med ; 14(7): e12435, 2022 07 07.
Article in English | MEDLINE | ID: mdl-35620963

ABSTRACT

Bacteriophages (phages) are selective viral predators of bacteria. Abundant and ubiquitous in nature, phages can be used to treat bacterial infections (phage therapy), including refractory infections and those resistant to antibiotics. However, despite an abundance of anecdotal evidence of efficacy, significant hurdles remain before routine implementation of phage therapy into medical practice, including a dearth of robust clinical trial data. Phage-bacterium interactions are complex and diverse, characterized by co-evolution trajectories that are significantly influenced by the environments in which they occur (mammalian body sites, water, soil, etc.). An understanding of the molecular mechanisms underpinning these dynamics is essential for successful clinical translation. This review aims to cover key aspects of bacterium-phage interactions that affect bacterial killing by describing the most relevant published literature and detailing the current knowledge gaps most likely to influence therapeutic success.


Subject(s)
Bacterial Infections , Bacteriophages , Phage Therapy , Animals , Anti-Bacterial Agents/therapeutic use , Bacteria , Bacterial Infections/microbiology , Bacterial Infections/therapy , Mammals
4.
J Biol Chem ; 296: 100286, 2021.
Article in English | MEDLINE | ID: mdl-33450228

ABSTRACT

Pathogenic microorganisms often reside in glycan-based biofilms. Concentration and chain length distribution of these mostly anionic exopolysaccharides (EPS) determine the overall biophysical properties of a biofilm and result in a highly viscous environment. Bacterial communities regulate this biofilm state via intracellular small-molecule signaling to initiate EPS synthesis. Reorganization or degradation of this glycan matrix, however, requires the action of extracellular glycosidases. So far, these were mainly described for bacteriophages that must degrade biofilms for gaining access to host bacteria. The plant pathogen Pantoea stewartii (P. stewartii) encodes the protein WceF within its EPS synthesis cluster. WceF has homologs in various biofilm forming plant pathogens of the Erwinia family. In this work, we show that WceF is a glycosidase active on stewartan, the main P. stewartii EPS biofilm component. WceF has remarkable structural similarity with bacteriophage tailspike proteins (TSPs). Crystal structure analysis showed a native trimer of right-handed parallel ß-helices. Despite its similar fold, WceF lacks the high stability found in bacteriophage TSPs. WceF is a stewartan hydrolase and produces oligosaccharides, corresponding to single stewartan repeat units. However, compared with a stewartan-specific glycan hydrolase of bacteriophage origin, WceF showed lectin-like autoagglutination with stewartan, resulting in notably slower EPS cleavage velocities. This emphasizes that the bacterial enzyme WceF has a role in P. stewartii biofilm glycan matrix reorganization clearly different from that of a bacteriophage exopolysaccharide depolymerase.


Subject(s)
Bacterial Proteins/chemistry , Biofilms/growth & development , Glycoside Hydrolases/chemistry , Pantoea/enzymology , Polysaccharides, Bacterial/chemistry , Viral Tail Proteins/chemistry , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Bacteriophages/chemistry , Bacteriophages/enzymology , Binding Sites , Carbohydrate Sequence , Cloning, Molecular , Crystallography, X-Ray , Escherichia coli/genetics , Escherichia coli/metabolism , Gene Expression , Genetic Vectors/chemistry , Genetic Vectors/metabolism , Glycoside Hydrolases/genetics , Glycoside Hydrolases/metabolism , Models, Molecular , Oligosaccharides/chemistry , Oligosaccharides/metabolism , Pantoea/genetics , Plants/microbiology , Polysaccharides, Bacterial/metabolism , Protein Binding , Protein Conformation, alpha-Helical , Protein Conformation, beta-Strand , Protein Interaction Domains and Motifs , Protein Multimerization , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Structural Homology, Protein , Viral Tail Proteins/genetics , Viral Tail Proteins/metabolism
5.
Front Microbiol ; 11: 510638, 2020.
Article in English | MEDLINE | ID: mdl-33072001

ABSTRACT

Bacteriophages use a large number of different bacterial cell envelope structures as receptors for surface attachment. As a consequence, bacterial surfaces represent a major control point for the defense against phage attack. One strategy for phage population control is the production of outer membrane vesicles (OMVs). In Gram-negative host bacteria, O-antigen-specific bacteriophages address lipopolysaccharide (LPS) to initiate infection, thus relying on an essential outer membrane glycan building block as receptor that is constantly present also in OMVs. In this work, we have analyzed interactions of Salmonella (S.) bacteriophage P22 with OMVs. For this, we isolated OMVs that were formed in large amounts during mechanical cell lysis of the P22 S. Typhimurium host. In vitro, these OMVs could efficiently reduce the number of infective phage particles. Fluorescence spectroscopy showed that upon interaction with OMVs, bacteriophage P22 released its DNA into the vesicle lumen. However, only about one third of the phage P22 particles actively ejected their genome. For the larger part, no genome release was observed, albeit the majority of phages in the system had lost infectivity towards their host. With OMVs, P22 ejected its DNA more rapidly and could release more DNA against elevated osmotic pressures compared to DNA release triggered with protein-free LPS aggregates. This emphasizes that OMV composition is a key feature for the regulation of infective bacteriophage particles in the system.

6.
Chemistry ; 26(32): 7263-7273, 2020 Jun 05.
Article in English | MEDLINE | ID: mdl-32189378

ABSTRACT

Broad and unspecific use of antibiotics accelerates spread of resistances. Sensitive and robust pathogen detection is thus important for a more targeted application. Bacteriophages contain a large repertoire of pathogen-binding proteins. These tailspike proteins (TSP) often bind surface glycans and represent a promising design platform for specific pathogen sensors. We analysed bacteriophage Sf6 TSP that recognizes the O-polysaccharide of dysentery-causing Shigella flexneri to develop variants with increased sensitivity for sensor applications. Ligand polyrhamnose backbone conformations were obtained from 2D 1 H,1 H-trNOESY NMR utilizing methine-methine and methine-methyl correlations. They agreed well with conformations obtained from molecular dynamics (MD), validating the method for further predictions. In a set of mutants, MD predicted ligand flexibilities that were in good correlation with binding strength as confirmed on immobilized S. flexneri O-polysaccharide (PS) with surface plasmon resonance. In silico approaches combined with rapid screening on PS surfaces hence provide valuable strategies for TSP-based pathogen sensor design.


Subject(s)
Bacteriophages/chemistry , O Antigens/chemistry , Shigella flexneri/chemistry , Viral Tail Proteins/chemistry , Binding Sites , Glycoside Hydrolases , Ligands , Molecular Dynamics Simulation , Viral Tail Proteins/metabolism
7.
Biomacromolecules ; 20(10): 3842-3854, 2019 10 14.
Article in English | MEDLINE | ID: mdl-31478651

ABSTRACT

Biofilms are complex mixtures of proteins, DNA, and polysaccharides surrounding bacterial communities as protective barriers that can be biochemically modified during the bacterial life cycle. However, their compositional heterogeneity impedes a precise analysis of the contributions of individual matrix components to the biofilm structural organization. To investigate the structural properties of glycan-based biofilms, we analyzed the diffusion dynamics of nanometer-sized objects in matrices of the megadalton-sized anionic polysaccharide, stewartan, the major biofilm component of the plant pathogen, Pantoea stewartii. Fluorescence correlation spectroscopy and single-particle tracking of nanobeads and bacteriophages indicated notable subdiffusive dynamics dependent on probe size and stewartan concentration, in contrast to free diffusion of small molecules. Stewartan enzymatic depolymerization by bacteriophage tailspike proteins rapidly restored unhindered diffusion. We, thus, hypothesize that the glycan polymer stewartan determines the major physicochemical properties of the biofilm, which acts as a selective diffusion barrier for nanometer-sized objects and can be controlled by enzymes.


Subject(s)
Bacteriophages/metabolism , Nanoparticles/metabolism , Polysaccharides/metabolism , Biofilms , Pantoea/metabolism , Polymers/metabolism , Polysaccharides, Bacterial/metabolism
8.
J Biol Chem ; 294(31): 11751-11761, 2019 08 02.
Article in English | MEDLINE | ID: mdl-31189652

ABSTRACT

Myoviruses, bacteriophages with T4-like architecture, must contract their tails prior to DNA release. However, quantitative kinetic data on myovirus particle opening are lacking, although they are promising tools in bacteriophage-based antimicrobial strategies directed against Gram-negative hosts. For the first time, we show time-resolved DNA ejection from a bacteriophage with a contractile tail, the multi-O-antigen-specific Salmonella myovirus Det7. DNA release from Det7 was triggered by lipopolysaccharide (LPS) O-antigen receptors and notably slower than in noncontractile-tailed siphoviruses. Det7 showed two individual kinetic steps for tail contraction and particle opening. Our in vitro studies showed that highly specialized tailspike proteins (TSPs) are necessary to attach the particle to LPS. A P22-like TSP confers specificity for the Salmonella Typhimurium O-antigen. Moreover, crystal structure analysis at 1.63 Šresolution confirmed that Det7 recognized the Salmonella Anatum O-antigen via an ϵ15-like TSP, DettilonTSP. DNA ejection triggered by LPS from either host showed similar velocities, so particle opening is thus a process independent of O-antigen composition and the recognizing TSP. In Det7, at permissive temperatures TSPs mediate O-antigen cleavage and couple cell surface binding with DNA ejection, but no irreversible adsorption occurred at low temperatures. This finding was in contrast to short-tailed Salmonella podoviruses, illustrating that tailed phages use common particle-opening mechanisms but have specialized into different infection niches.


Subject(s)
DNA, Viral/metabolism , Salmonella Phages/metabolism , Salmonella typhimurium/virology , Crystallography, X-Ray , Glycoside Hydrolases , Lipopolysaccharides/pharmacology , O Antigens/metabolism , Protein Structure, Tertiary , Salmonella Phages/drug effects , Salmonella typhimurium/metabolism , Viral Tail Proteins/chemistry , Viral Tail Proteins/metabolism
9.
Adv Sci (Weinh) ; 5(8): 1800432, 2018 Aug.
Article in English | MEDLINE | ID: mdl-30128249

ABSTRACT

Biomembranes are constantly remodeled and in cells, these processes are controlled and modulated by an assortment of membrane proteins. Here, it is shown that such remodeling can also be induced by photoresponsive molecules. The morphological control of giant vesicles in the presence of a water-soluble ortho-tetrafluoroazobenzene photoswitch (F-azo) is demonstrated and it is shown that the shape transformations are based on an increase in membrane area and generation of spontaneous curvature. The vesicles exhibit budding and the buds can be retracted by using light of a different wavelength. In the presence of F-azo, the membrane area can increase by more than 5% as assessed from vesicle electrodeformation. To elucidate the underlying molecular mechanism and the partitioning of F-azo in the membrane, molecular dynamics simulations are employed. Comparison with theoretically calculated shapes reveals that the budded shapes are governed by curvature elasticity, that the spontaneous curvature can be decomposed into a local and a nonlocal contribution, and that the local spontaneous curvature is about 1/(2.5 µm). The results show that exo- and endocytotic events can be controlled by light and that these photoinduced processes provide an attractive method to change membrane area and morphology.

10.
Viruses ; 10(8)2018 08 15.
Article in English | MEDLINE | ID: mdl-30111705

ABSTRACT

Bacteriophage research is gaining more importance due to increasing antibiotic resistance. However, for treatment with bacteriophages, diagnostics have to be improved. Bacteriophages carry adhesion proteins, which bind to the bacterial cell surface, for example tailspike proteins (TSP) for specific recognition of bacterial O-antigen polysaccharide. TSP are highly stable proteins and thus might be suitable components for the integration into diagnostic tools. We used the TSP of bacteriophage Sf6 to establish two applications for detecting Shigella flexneri (S. flexneri), a highly contagious pathogen causing dysentery. We found that Sf6TSP not only bound O-antigen of S. flexneri serotype Y, but also the glucosylated O-antigen of serotype 2a. Moreover, mass spectrometry glycan analyses showed that Sf6TSP tolerated various O-acetyl modifications on these O-antigens. We established a microtiter plate-based ELISA like tailspike adsorption assay (ELITA) using a Strep-tag®II modified Sf6TSP. As sensitive screening alternative we produced a fluorescently labeled Sf6TSP via coupling to an environment sensitive dye. Binding of this probe to the S. flexneri O-antigen Y elicited a fluorescence intensity increase of 80% with an emission maximum in the visible light range. The Sf6TSP probes thus offer a promising route to a highly specific and sensitive bacteriophage TSP-based Shigella detection system.


Subject(s)
Bacterial Typing Techniques , Bacteriophages/chemistry , Biological Assay , O Antigens/chemistry , Podoviridae/chemistry , Shigella flexneri/isolation & purification , Viral Tail Proteins/chemistry , Bacteriophages/genetics , Bacteriophages/metabolism , Carbohydrate Sequence , Cloning, Molecular , Enzyme-Linked Immunosorbent Assay , Escherichia coli/genetics , Escherichia coli/metabolism , Fluorescent Dyes/chemistry , Gene Expression , Genetic Vectors/chemistry , Genetic Vectors/metabolism , Glycoside Hydrolases , Models, Molecular , O Antigens/metabolism , Oxadiazoles/chemistry , Podoviridae/genetics , Podoviridae/metabolism , Protein Binding , Protein Structure, Secondary , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Serogroup , Shigella flexneri/chemistry , Shigella flexneri/metabolism , Staining and Labeling/methods , Viral Tail Proteins/genetics , Viral Tail Proteins/metabolism
11.
J Am Chem Soc ; 140(33): 10447-10455, 2018 08 22.
Article in English | MEDLINE | ID: mdl-30044908

ABSTRACT

The principles of protein-glycan binding are still not well understood on a molecular level. Attempts to link affinity and specificity of glycan recognition to structure suffer from the general lack of model systems for experimental studies and the difficulty to describe the influence of solvent. We have experimentally and computationally addressed energetic contributions of solvent in protein-glycan complex formation in the tailspike protein (TSP) of E. coli bacteriophage HK620. HK620TSP is a 230 kDa native trimer of right-handed, parallel beta-helices that provide extended, rigid binding sites for bacterial cell surface O-antigen polysaccharides. A set of high-affinity mutants bound hexa- or pentasaccharide O-antigen fragments with very similar affinities even though hexasaccharides introduce an additional glucose branch into an occluded protein surface cavity. Remarkably different thermodynamic binding signatures were found for different mutants; however, crystal structure analyses indicated that no major oligosaccharide or protein topology changes had occurred upon complex formation. This pointed to a solvent effect. Molecular dynamics simulations using a mobility-based approach revealed an extended network of solvent positions distributed over the entire oligosaccharide binding site. However, free energy calculations showed that a small water network inside the glucose-binding cavity had the most notable influence on the thermodynamic signature. The energy needed to displace water from the glucose binding pocket depended on the amino acid at the entrance, in agreement with the different amounts of enthalpy-entropy compensation found for introducing glucose into the pocket in the different mutants. Studies with small molecule drugs have shown before that a few active water molecules can control protein complex formation. HK620TSP oligosaccharide binding shows that similar fundamental principles also apply for glycans, where a small number of water molecules can dominate the thermodynamic signature in an extended binding site.


Subject(s)
Oligosaccharides/chemistry , Proteins/chemistry , Solvents/chemistry , Thermodynamics , Binding Sites , Coliphages/chemistry , Crystallography, X-Ray , Glycoside Hydrolases , Molecular Dynamics Simulation , Protein Conformation , Viral Tail Proteins/chemistry
12.
Viruses ; 10(6)2018 05 29.
Article in English | MEDLINE | ID: mdl-29843473

ABSTRACT

Gram-negative bacteria protect themselves with an outermost layer containing lipopolysaccharide (LPS). O-antigen-specific bacteriophages use tailspike proteins (TSP) to recognize and cleave the O-polysaccharide part of LPS. However, O-antigen composition and structure can be highly variable depending on the environmental conditions. It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance. In this work, we have analyzed how LPS preparations in vitro trigger particle opening and DNA ejection from the E. coli podovirus HK620. Fluorescence-based monitoring of DNA release showed that HK620 phage particles in vitro ejected their genome at velocities comparable to those found for other podoviruses. Moreover, we found that HK620 irreversibly adsorbed to the LPS receptor via its TSP at restrictive low temperatures, without opening the particle but could eject its DNA at permissive temperatures. DNA ejection was solely stimulated by LPS, however, the composition of the O-antigen dictated whether the LPS receptor could start the DNA release from E. coli phage HK620 in vitro. This finding can be significant when optimizing bacteriophage mixtures for therapy, where in natural environments O-antigen structures may rapidly change.


Subject(s)
DNA, Viral/metabolism , Lipopolysaccharides/pharmacology , Podoviridae/drug effects , Podoviridae/genetics , Bacteriophage P22/genetics , Escherichia coli/virology , Glycoside Hydrolases , Temperature , Viral Tail Proteins/metabolism
13.
Mol Microbiol ; 105(3): 353-357, 2017 Aug.
Article in English | MEDLINE | ID: mdl-28618013

ABSTRACT

Tailed bacteriophages specific for Gram-negative bacteria encounter lipopolysaccharide (LPS) during the first infection steps. Yet, it is not well understood how biochemistry of these initial interactions relates to subsequent events that orchestrate phage adsorption and tail rearrangements to initiate cell entry. For many phages, long O-antigen chains found on the LPS of smooth bacterial strains serve as essential receptor recognized by their tailspike proteins (TSP). Many TSP are depolymerases and O-antigen cleavage was described as necessary step for subsequent orientation towards a secondary receptor. However, O-antigen specific host attachment must not always come along with O-antigen degradation. In this issue of Molecular Microbiology Prokhorov et al. report that coliphage G7C carries a TSP that deacetylates O-antigen but does not degrade it, whereas rough strains or strains lacking O-antigen acetylation remain unaffected. Bacteriophage G7C specifically functionalizes its tail by attaching the deacetylase TSP directly to a second TSP that is nonfunctional on the host's O-antigen. This challenges the view that bacteriophages use their TSP only to clear their way to a secondary receptor. Rather, O-antigen specific phages may employ enzymatically active TSP as a tool for irreversible LPS membrane binding to initiate subsequent infection steps.


Subject(s)
O Antigens/metabolism , Viral Tail Proteins/metabolism , Bacteriophage P22/metabolism , Bacteriophages/physiology , Lipopolysaccharides/metabolism , O Antigens/physiology , Salmonella typhimurium/metabolism , Structure-Activity Relationship
14.
BMC Microbiol ; 16: 207, 2016 09 07.
Article in English | MEDLINE | ID: mdl-27604475

ABSTRACT

BACKGROUND: Non-typhoid Salmonella Typhimurium (S. Typhimurium) accounts for a high number of registered salmonellosis cases, and O-serotyping is one important tool for monitoring epidemiology and spread of the disease. Moreover, variations in glucosylated O-antigens are related to immunogenicity and spread in the host. However, classical autoagglutination tests combined with the analysis of specific genetic markers cannot always reliably register phase variable glucose modifications expressed on Salmonella O-antigens and additional tools to monitor O-antigen glucosylation phenotypes of S. Typhimurium would be desirable. RESULTS: We developed a test for the phase variable O-antigen glucosylation state of S. Typhimurium using the tailspike proteins (TSP) of Salmonella phages 9NA and P22. We used this ELISA like tailspike adsorption (ELITA) assay to analyze a library of 44 Salmonella strains. ELITA was successful in discriminating strains that carried glucose 1-6 linked to the galactose of O-polysaccharide backbone (serotype O1) from non-glucosylated strains. This was shown by O-antigen compositional analyses of the respective strains with mass spectrometry and capillary electrophoresis. The ELITA test worked rapidly in a microtiter plate format and was highly O-antigen specific. Moreover, TSP as probes could also detect glucosylated strains in flow cytometry and distinguish multiphasic cultures differing in their glucosylation state. CONCLUSIONS: Tailspike proteins contain large binding sites with precisely defined specificities and are therefore promising tools to be included in serotyping procedures as rapid serotyping agents in addition to antibodies. In this study, 9NA and P22TSP as probes could specifically distinguish glucosylation phenotypes of Salmonella on microtiter plate assays and in flow cytometry. This opens the possibility for flow sorting of cell populations for subsequent genetic analyses or for monitoring phase variations during large scale O-antigen preparations necessary for vaccine production.


Subject(s)
O Antigens/analysis , Salmonella typhimurium/isolation & purification , Serotyping/methods , Viral Tail Proteins/analysis , Binding Sites , Electrophoresis, Capillary , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Glycoside Hydrolases , Lipopolysaccharides/analysis , Oligosaccharides/analysis , Phenotype , Polysaccharides/analysis , Salmonella Phages , Salmonella typhimurium/metabolism , Species Specificity , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Virus Diseases
15.
J Am Chem Soc ; 138(29): 9109-18, 2016 07 27.
Article in English | MEDLINE | ID: mdl-27045683

ABSTRACT

Understanding interactions of bacterial surface polysaccharides with receptor protein scaffolds is important for the development of antibiotic therapies. The corresponding protein recognition domains frequently form low-affinity complexes with polysaccharides that are difficult to address with experimental techniques due to the conformational flexibility of the polysaccharide. In this work, we studied the tailspike protein (TSP) of the bacteriophage Sf6. Sf6TSP binds and hydrolyzes the high-rhamnose, serotype Y O-antigen polysaccharide of the Gram-negative bacterium Shigella flexneri (S. flexneri) as a first step of bacteriophage infection. Spectroscopic analyses and enzymatic cleavage assays confirmed that Sf6TSP binds long stretches of this polysaccharide. Crystal structure analysis and saturation transfer difference (STD) NMR spectroscopy using an enhanced method to interpret the data permitted the detailed description of affinity contributions and flexibility in an Sf6TSP-octasaccharide complex. Dodecasaccharide fragments corresponding to three repeating units of the O-antigen in complex with Sf6TSP were studied computationally by molecular dynamics simulations. They showed that distortion away from the low-energy solution conformation found in the octasaccharide complex is necessary for ligand binding. This is in agreement with a weak-affinity functional polysaccharide-protein contact that facilitates correct placement and thus hydrolysis of the polysaccharide close to the catalytic residues. Our simulations stress that the flexibility of glycan epitopes together with a small number of specific protein contacts provide the driving force for Sf6TSP-polysaccharide complex formation in an overall weak-affinity interaction system.


Subject(s)
Bacteriophages , Molecular Dynamics Simulation , O Antigens/metabolism , Shigella flexneri/chemistry , Viral Tail Proteins/metabolism , Binding Sites , Glycoside Hydrolases , O Antigens/chemistry , Protein Binding , Protein Conformation , Viral Tail Proteins/chemistry
16.
J Phys Chem B ; 118(9): 2523-34, 2014 Mar 06.
Article in English | MEDLINE | ID: mdl-24559142

ABSTRACT

O-Antigen polysaccharides constitute the outer protective layer of most Gram-negative bacteria, important for the bacterium's survival and adaption within its host. Although important for many functions, the three-dimensional structure of the dense polysaccharide coat remains to be elucidated. In this study, we present a systematic numerical investigation of O-antigen polysaccharide chains of Shigella flexneri serotype Y composed of one up to four tetrasaccharide repeat units. To bridge the gap between atomistic and coarse-grained levels of description, we employ a genuine multiscale modeling approach. It reveals that even for a few repeat units polymer-like flexibility emerges, which is furthermore complemented by extreme, hairpin-like conformations. These can facilitate the formation of metastable compact states, but this conclusion depends sensitively on the force field used to model the carbohydrates. Thus, our computational analysis represents an essential prerequisite for developing reliable coarse-grained models that may help visualizing changes in O-antigen coat morphology upon variations in chain length distribution or chemical composition of the polysaccharide characterizing a certain serotype.


Subject(s)
O Antigens/chemistry , Shigella flexneri/metabolism , Molecular Dynamics Simulation , O Antigens/metabolism , Serotyping , Thermodynamics
17.
Glycobiology ; 23(4): 486-94, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23292517

ABSTRACT

Bacteriophage P22 recognizes O-antigen polysaccharides of Salmonella enterica subsp. enterica (S.) with its tailspike protein (TSP). In the serovars S. Typhimurium, S. Enteritidis, and S. Paratyphi A, the tetrasaccharide repeat units of the respective O-antigens consist of an identical main chain trisaccharide but different 3,6-dideoxyhexose substituents. Here, the epimers abequose, tyvelose and paratose determine the specific serotype. P22 TSP recognizes O-antigen octasaccharides in an extended binding site with a single 3,6-dideoxyhexose binding pocket. We have isolated S. Paratyphi A octasaccharides which were not available previously and determined the crystal structure of their complex with P22 TSP. We discuss our data together with crystal structures of complexes with S. Typhimurium and S. Enteritidis octasaccharides determined earlier. Isothermal titration calorimetry showed that S. Paratyphi A octasaccharide binds P22 TSP less tightly, with a difference in binding free energy of ∼7 kJ mol(-1) at 20°C compared with S. Typhimurium and S. Enteritidis octasaccharides. Individual protein-carbohydrate contacts were probed by amino acid replacements showing that the dideoxyhexose pocket contributes to binding of all three serotypes. However, S. Paratyphi A octasaccharides bind in a conformation with an energetically unfavorable ϕ/ψ glycosidic bond angle combination. In contrast, octasaccharides from the other serotypes bind as solution-like conformers. Two water molecules are conserved in all P22 TSP complexes with octasaccharides of different serotypes. They line the dideoxyhexose binding pocket and force the S. Paratyphi A octasaccharides to bind as nonsolution conformers. This emphasizes the role of solvent as part of carbohydrate binding sites.


Subject(s)
Bacteriophage P22/chemistry , O Antigens/chemistry , Salmonella paratyphi A/chemistry , Viral Tail Proteins/chemistry , Amino Acid Sequence , Binding Sites , Carbohydrate Conformation , Carbohydrate Sequence , Glycoside Hydrolases , Hexoses/chemistry , Molecular Docking Simulation , Molecular Sequence Data , Mutation , O Antigens/metabolism , Protein Binding , Salmonella paratyphi A/virology , Viral Tail Proteins/genetics , Viral Tail Proteins/metabolism
18.
Glycobiology ; 23(1): 59-68, 2013 Jan.
Article in English | MEDLINE | ID: mdl-22923442

ABSTRACT

Bacteriophage HK620 recognizes and cleaves the O-antigen polysaccharide of Escherichia coli serogroup O18A1 with its tailspike protein (TSP). HK620TSP binds hexasaccharide fragments with low affinity, but single amino acid exchanges generated a set of high-affinity mutants with submicromolar dissociation constants. Isothermal titration calorimetry showed that only small amounts of heat were released upon complex formation via a large number of direct and solvent-mediated hydrogen bonds between carbohydrate and protein. At room temperature, association was both enthalpy- and entropy-driven emphasizing major solvent rearrangements upon complex formation. Crystal structure analysis showed identical protein and sugar conformers in the TSP complexes regardless of their hexasaccharide affinity. Only in one case, a TSP mutant bound a different hexasaccharide conformer. The extended sugar binding site could be dissected in two regions: first, a hydrophobic pocket at the reducing end with minor affinity contributions. Access to this site could be blocked by a single aspartate to asparagine exchange without major loss in hexasaccharide affinity. Second, a region where the specific exchange of glutamate for glutamine created a site for an additional water molecule. Side-chain rearrangements upon sugar binding led to desolvation and additional hydrogen bonding which define this region of the binding site as the high-affinity scaffold.


Subject(s)
Coliphages/metabolism , Oligosaccharides/metabolism , Viral Tail Proteins/chemistry , Amino Acids , Asparagine/genetics , Asparagine/metabolism , Aspartic Acid/genetics , Aspartic Acid/metabolism , Binding Sites , Catalytic Domain , Crystallography, X-Ray , Glycoside Hydrolases , Hydrogen Bonding , Models, Molecular , O Antigens/chemistry , O Antigens/metabolism , Oligosaccharides/chemistry , Protein Conformation , Surface Properties , Thermodynamics , Viral Tail Proteins/genetics , Viral Tail Proteins/metabolism
19.
Carbohydr Res ; 357: 118-25, 2012 Aug 01.
Article in English | MEDLINE | ID: mdl-22704196

ABSTRACT

We have analyzed the O-antigen polysaccharide of the previously uncharacterized Escherichia coli strain TD2158 which is a host of bacteriophage HK620. This bacteriophage recognizes and cleaves the polysaccharide with its tailspike protein (TSP). The polysaccharide preparation as well as oligosaccharides obtained from HK620TSP endoglycosidase digests were analyzed with NMR spectroscopy. Additionally, sugar analysis was performed on the O-antigen polysaccharide and MALDI-TOF MS was used in oligosaccharide analysis. The present study revealed a heterogeneous polysaccharide with a hexasaccharide repeating unit of the following structure: α-D-Glcp-(1→6|) →2)-α-L-Rhap-91→6)-α-D-Glcp-(1→4)-α-D-Ga|lp-(1→3)-α-D-GlcpNAc-(1→ ß-D-Glcp/ß-D-GlcpNAc-(1→3) A repeating unit with a D-GlcNAc substitution of D-Gal has been described earlier as characteristic for serogroup O18A1. Accordingly, we termed repeating units with D-Glc substitution at D-Gal as O18A2. NMR analyses of the polysaccharide confirmed that O18A1- and O18A2-type repeats were present in a 1:1 ratio. However, HK620TSP preferentially bound the D-GlcNAc-substituted O18A1-type repeating units in its high affinity binding pocket with a dissociation constant of 140 µM and disfavored the O18A2-type having a ß-D-Glcp-(1→3)-linked group. As a result, in hexasaccharide preparations, O18A1 and O18A2 repeats were present in a 9:1 ratio stressing the clear preference of O18A1-type repeats to be cleaved by HK620TSP.


Subject(s)
Escherichia coli/chemistry , Glycoside Hydrolases/chemistry , O Antigens/chemistry , Podoviridae/physiology , Viral Tail Proteins/chemistry , Carbohydrate Conformation , Carbohydrate Sequence , Catalytic Domain , Escherichia coli/virology , Hydrogen Bonding , Magnetic Resonance Spectroscopy , Models, Molecular , Podoviridae/enzymology , Surface Properties
20.
Mol Microbiol ; 83(6): 1244-53, 2012 Mar.
Article in English | MEDLINE | ID: mdl-22364412

ABSTRACT

Bacteriophages use specific tail proteins to recognize host cells. It is still not understood to molecular detail how the signal is transmitted over the tail to initiate infection. We have analysed in vitro DNA ejection in long-tailed siphovirus 9NA and short-tailed podovirus P22 upon incubation with Salmonella typhimurium lipopolysaccharide (LPS). We showed for the first time that LPS alone was sufficient to elicit DNA release from a siphovirus in vitro. Crystal structure analysis revealed that both phages use similar tailspike proteins for LPS recognition. Tailspike proteins hydrolyse LPS O antigen to position the phage on the cell surface. Thus we were able to compare in vitro DNA ejection processes from two phages with different morphologies with the same receptor under identical experimental conditions. Siphovirus 9NA ejected its DNA about 30 times faster than podovirus P22. DNA ejection is under control of the conformational opening of the particle and has a similar activation barrier in 9NA and P22. Our data suggest that tail morphology influences the efficiencies of particle opening given an identical initial receptor interaction event.


Subject(s)
Bacteriophage P22/metabolism , DNA, Viral/metabolism , Lipopolysaccharides/metabolism , Receptors, Virus/metabolism , Salmonella Phages/metabolism , Salmonella typhimurium/virology , Viral Tail Proteins/metabolism , Bacteriophage P22/chemistry , Bacteriophage P22/genetics , Caliciviridae/chemistry , Caliciviridae/genetics , Caliciviridae/metabolism , DNA, Viral/genetics , Protein Binding , Salmonella Phages/chemistry , Salmonella Phages/genetics , Salmonella typhimurium/metabolism , Viral Structures/chemistry , Viral Structures/genetics , Viral Structures/metabolism , Viral Tail Proteins/chemistry , Viral Tail Proteins/genetics
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