ABSTRACT
BACKGROUND: Metallothioneins (MTs) gene polymorphisms have been associated with the ability of free radical scavenging and detoxification of heavy metals leading to cancer development. Our aim was to revisit, in a Brazilian population, single-nucleotide polymorphisms (SNPs) of the MT gene family previously associated with oral squamous cell carcinoma (OSCC). MATERIAL AND METHODS: A case-control investigation with 28 OSCC patients and 45 controls was conducted, using conventional risk factors (tobacco use and alcohol consumption) as covariates. SNPs genotyping for rs8052334 (MT1B), rs964372 (MT1B), and rs1610216 (MT2A) was performed by PCR-RFLP, and SNPs for rs11076161 (MT1A) were analyzed by TaqMan assay. RESULTS: The only SNP associated with increased risk for OSCC was the MT-1A AA genotype (OR = 4.7; p = 0.01). We have also evidenced for the first time a significant linkage disequilibrium between the SNPs of MT-2A and MT-1A in this population with the highest frequency (30%) of the unfavorable haplotype G/A/C/T (rs1610216 / rs11076161 / rs964372 / rs8052334) of MT gene polymorphisms (OR = 6.2; p = 0.04). Interestingly, after removing the effects of conventional risk factors, we have uncovered the significance of the AA genotype of the rs11076161 with increased odds of 19-fold higher towards OSCC development. CONCLUSIONS: This is the first demonstration that a significant linkage disequilibrium among gene polymorphisms of the MT family may affect susceptibility to oral cancer, which is conditioned by the G/A/C/T haplotype (rs1610216/rs11076161/rs964372/ rs8052334) and the MT-1A gene polymorphism has a potential clinical utility for the OSCC risk assessment.
Subject(s)
Carcinoma, Squamous Cell , Head and Neck Neoplasms , Mouth Neoplasms , Brazil , Carcinoma, Squamous Cell/genetics , Case-Control Studies , Genetic Predisposition to Disease , Genotype , Humans , Metallothionein/genetics , Mouth Neoplasms/genetics , Polymorphism, Single Nucleotide , Risk Factors , Squamous Cell Carcinoma of Head and NeckABSTRACT
Avaliou-se o efeito da substituição do milho por farelo de manga em dietas para ovinos. As características avaliadas foram: consumo e digestibilidade aparente da matéria seca (MS), da proteína bruta (PB), da fibra em detergente neutro (FDN), da matéria orgânica (MO), do extrato etéreo (EE), da energia bruta (EB), da energia digestível (ED), dos carboidratos não fibrosos (CNF) e dos carboidratos totais (CT). Vinte e quatro cordeiros Santa Inês foram distribuídos em delineamento inteiramente ao acaso, com quatro tratamentos, constituídos pelas porcentagens de substituição do milho pelo farelo de manga - 0, 33, 66 e 100% -, com seis repetições. A matéria seca fecal foi estimada utilizando-se o indicador LIPE®. Não houve efeito da inclusão do farelo de manga sobre o consumo dos nutrientes e nos coeficientes de digestibilidade. Os consumos de EB e ED, em kcal/gMS, aumentaram linearmente com a inclusão do farelo de manga. O farelo de manga pode substituir o milho em dietas para ovinos, sem comprometer o consumo e a digestibilidade dos nutrientes, indicando a possibilidade de uso na dieta desses animais como alimento alternativo.
The effect of replacing corn meal with mango meal in diets of feedlot lambs was evaluated. Apparent digestibility and intakes of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), organic matter (OM) (EE), gross energy (GE), digestible energy (DE), non-fiber carbohydrates (NFC) and total carbohydrates (TC) were evaluated. Twenty-four Santa Inês lambs were distributed in a randomized design with four treatments, consisting of the replacement levels of corn meal by mango (0, 33, 66 and 100%) with six replicates. The fecal dry matter was estimated using LIPE ® external marker. There is no effect of mango meal inclusion levels on the intake and apparent digestibility. The intakes of GE and DE in kcal/gDM increase linearly with mango meal inclusion. The mango meal can replace corn in diets for lambs without compromising the intake and digestibility of nutrients, indicating potential as an alternative feed.
