Estudo de incidência de acometimentos cardiovasculares e pulmonares causados pelo cigarro eletrônico: uma revisão de escopo / Incidence study of cardiovascular and pulmonary disorders caused by electronic cigarettes: a scope review

Introdução: O uso de cigarros eletrônicos (CE) tem aumentado, mas sem um estudo aprofundado sobre seus efeitos na saúde cardiovascular e pulmonar. Uma pergunta norteadora foi elaborada: Os cigarros eletrônicos causam acometimentos cardiovasculares e pulmonares? Esta revisão de escopo pontua a existência dos acometimentos cardiovasculares e pulmonares causados pelo uso de cigarros eletrônicos. Metodologia: Bancos de dados: MEDLINE-Pubmed, LILACS e Web of Science. Descritores: "Electronic Nicotine Delivery System", "E-Cigarette Vapor", "COPD", "Lung Neoplasms", "Myocardial Infarction" e "Pneumonia". Primeira busca: 28 artigos. Critérios de Exclusão: artigos que não se encaixam na classificação de estudos primários e estudos duplicados. Busca final: 6 artigos, descritos na íntegra. Resultados: Dos artigos encontrados: 83,3% (EUA); 16,7% (Coreia do Sul); 16,7% estudo transversal; 16,7% estudo longitudinal; 33,3% ensaio clínico randomizado com pessoas; 33,3% estudos de coorte. Discussão: Os artigos demonstraram que o CE pode estar associado a acometimentos cardiovasculares, como infarto e acidente vascular, apesar de não ser tão influente quando comparado ao cigarro tradicional. Quanto aos acometimentos pulmonares, não houve consenso entre os dados dos artigos pesquisados. Conclusão: Faz-se necessária a realização de mais pesquisas sobre o tema, devido a divergência entre os dados presentes na literatura.

Introduction: The use of electronic cigarettes (EC) has increased, but without an in-depth study on their effects on cardiovascular and pulmonary health. A guiding question was formulated: Do electronic cigarettes cause cardiovascular and pulmonary disorders? This scoping review points out the existence of cardiovascular and pulmonary disorders caused by the use of electronic cigarettes. Methodology: Databases: MEDLINE-Pubmed, LILACS and Web of Science. Descriptors: "Electronic Nicotine Delivery System", "E-Cigarette Vapor", "COPD", "Lung Neoplasms", "Myocardial Infarction" and "Pneumonia". First search: 28 articles. Exclusion Criteria: articles that do not fit into the classification of primary studies and duplicated studies. Final search: 6 articles, described in full. Results: Of the articles found: 83.3% (USA); 16.7% (South Korea); 16.7% cross-sectional study; 16.7% longitudinal study; 33.3% randomized clinical trial with people; 33.3% cohort studies. Discussion: The articles showed that the EC may be associated with cardiovascular disorders, such as infarction and stroke, although it is not as influential when compared to traditional cigarettes. As for pulmonary disorders, there was no consensus among the data from the researched articles. Conclusion: More research on the topic is necessary, due to the divergence between the presented data in the literature.

Spatially Coherent Tip-Enhanced Raman Spectroscopy Measurements of Electron-Phonon Interaction in a Graphene Device.

Coherence length (Lc) of the Raman scattering process in graphene as a function of Fermi energy is obtained with spatially coherent tip-enhanced Raman spectroscopy. Lc decreases when the Fermi energy is moved into the neutrality point, consistent with the concept of the Kohn anomaly within a ballistic transport regime. Since the Raman scattering involves electrons and phonons, the observed results can be rationalized either as due to unusually large variation of the longitudinal optical phonon group velocity vg, reaching twice the value for the longitudinal acoustic phonon, or due to changes in the electron energy uncertainty, both properties being important for optical and transport phenomena that might not be observable by any other technique.

First report of Neopestalotiopsis foedans causing pestalotia spot in leaf on coconut in Brazil.

The coconut (Cocos nucifera L., Arecaceae) is one of the most important tropical species used by humans. In Brazil, its cultivation has been expanding in the recent years (Souza et al. 2020) and many diseases have emerged. The pestalotia spot, caused by Pestalotiopsis guepinii (Desm.), is a leaf disease of the coconut characterized by elliptic lesions with defined dark borders varying in size from 3 to 5 mm (Cardoso et al. 2003). In January of 2018, leaves with symptoms of pestalotia spot were obtained from ten year old coconut plants "dwarf variety" in a commercial planting in the city of Neópolis (10°20'S/36°42'W), Sergipe, Brazil. Disease incidence was 80% on 60 plants observed. Twenty samples of symptomatic tissues were collected and disinfested for 2 min in 1% sodium hypochlorite, washed in sterile water, placed on PDA (potato dextrose agar), and incubated at 25 ± 1°C with a 12-h photoperiod for 4 days. Five isolates were obtained, and pure cultures deposited in Phytopathogen Collection of the Federal University of Alagoas, accession numbers: COUFAL0240 to COUFAL0244. Seven day old colonies grown on PDA at 25°C, were whitish with aerial mycelium on the surface and abundant production of black conidiomata. Conidia were fusiform, straight to slightly curved with five cells, three median cells with brown coloring being the second and third being darker and the apical and basal cells, hyaline. Fifty conidia were measured and varied in size from 20.02-24.26 x 5.37-7.50 µm. The conidia presented two to four apical appendages and one basal appendage (Fig. S1). The morphological characteristics coincide with the Neopestalotiopsis foedans (Sacc. & Ellis), Maharachchikumbura et al. (2014). Molecular identification was conducted using partial nucleotide sequences from the ITS (ITS1/ITS4) region (GenBank no. MT605375 to MT605379) and from the genes TUB2 (Bt2a/Bt2B) (no. MT634202 to MT634206) and TEF-1α (526F/1567R) (no. MT634197 to MT634201). Besides that, the isolates grouped with the ex-type N. foedans species (CGMCC 3.9123) in a phylogenetic tree of Bayesian inference using concatenated sequences (Fig. S2). The pathogenicity was confirmed on seedling from coconut plants "dwarf variety" maintained in a greenhouse. Four plants were used, being one as a control. Spore suspensions of 106 conidia mL-1 was prepared from a 7 days old culture (cultivated at 25ºC). Inoculations were performed by spraying the conidial suspension on two whole leaves per plant (wounded and unwounded). In the control, sterilized distilled water was used. Plants were incubated at 25 ± 1°C and 100% relative humidity. Ten days after inoculation, depressed and necrotic lesions were observed in 100% on the inoculated leaves with wound. No symptoms observed on unwounded leaves, nor in the control treatment. To complete Koch's postulates, the N. foedans fungus was successfully re-isolated from the symptomatic leaves and identified phenotypically in optical microscope. Neopestalotiopsis foedans has already been reported in Calliandra haematocephala (Hassk), Neodypsis decaryi (Jum.), Rhizophora mangle (L.), Thuja occidentalis and Psidium guajava (L.) (Saccardo, 1882; Maharachchikumbura et al. 2014, Solarte et al. 2018). However, this is first report of N. foedans causing leaf spot in coconut in the world. The pestalotia spot is commonly observed in Brazil in C. nucifera and should be considered an important disease for this culture, as this can significantly reduce its photosynthetic area.

Cost and effectiveness of 3-dimensionally printed model using three different printing layer parameters and two resins.

STATEMENT OF PROBLEM: When 3-dimensional printing casts, the operator can change the type of resin and the printing layer thickness, reducing the fabrication time. However, how these parameters affect the accuracy of 3-dimensionally printed casts is unknown. PURPOSE: The purpose of this in vitro study was to evaluate the accuracy of 3-dimensionally printed casts by using a stereolithography (SLA) 3-dimensional printer (Forms2) with 3 different layer thickness (25, 50, and 100 µm) and 2 different resins (Gray and Cast) and by comparing the time to obtain each cast. MATERIAL AND METHODS: One master cast was scanned, and a single file was printed several times. The printed casts were then scanned by using a laboratory scanner. The standard tessellation language (STL) files provided by the laboratory scanner were superimposed and compared by using a software program (Geomagic Control; 3D Systems). The 2-way ANOVA test was used for the trueness evaluation, followed by the Tukey test to identify differences among the groups (α=.05). RESULTS: No statistically significant differences in accuracy were found among the 3 different layers for either resin (P>.05). Printing time doubled as layer thickness decreased. CONCLUSIONS: This study showed that when printing casts, the fastest printing settings can be used without losing accuracy and that the laboratory digital workflow can be accelerated with selection of the resin and cast layer, as the type of resin and layer thickness did not influence the quality of the casts.

Prevalence of non-carious cervical lesions and orthodontic treatment: a retrospective study.

BACKGROUND: This study aimed to assess the distribution of non-carious cervical lesions (NCCLs) by tooth type, investigate the prevalence of NCCLs in patients undergoing orthodontic treatment, and identify the possible associated factors. MATERIAL AND METHODS: A total of 160 patients were enrolled in this retrospective study. Data on the following variables were collected from pre-and post-orthodontic treatment records: age, sex, Angle's malocclusion, facial pattern, number of activation sessions, compensatory treatment, and retreatment. Frontal, right and left lateral intraoral photographs of each patient were evaluated to identify the presence or absence of NCCLs in each tooth and assess the distribution of NCCLs in the 3840 teeth from the enrolled patients. Furthermore, patients were classified as NCCLs present, irrespective of the number of NCCLs on the teeth or NCCL absent. Bivariate and multivariate Poisson regression analyses with robust variance were used to assess the association between the NCCLs and each independent variable. Prevalence ratio and 95% confidence intervals were calculated and p < 0.05 was considered statistically significant. RESULTS: The prevalence of NCCLs before and after orthodontic treatment was 22.71% and 30.91%, respectively. Premolars were the most affected teeth, followed by the first molars, canines, and incisors. After statistical analysis, age was found to be the variable factor that influenced the prevalence ratio, with NCCL being the most prevalent when orthodontic treatment was performed in adulthood. CONCLUSIONS: Premolars were most commonly affected by NCCLs. Furthermore, age seemed to contribute to the increased prevalence of NCCLs in adults undergoing orthodontic treatment.

Performance of disabled swimmers in protocols or tests and competitions: a systematic review and meta-analysis.

Biomechanical and coordination measurements are useful tools to assess swimming performance. Regarding Paralympic swimming, function and technique make these measurements complex. The aim of this study was to perform a systematic review of studies on biomechanics, coordination and performance in disabled swimmers following swimming protocols and in competitions. Data sources: PubMed, EMBASE, ISI Web of Knowledge, SPORTDiscus and Academic Search Premier. We selected complete studies, published until June 2018. Eighteen studies satisfied the inclusion criteria and were selected for qualitative analysis; nine of these were included in the meta-analysis. Swimming speed and stroke length increase with less impact of physical, visual and intellectual impairment on performing specific swimming tasks. Stroke rate is more stable all through the sport classes than both swimming speed and stroke length. Most physically disabled swimmers adopt the catch-up coordination model. Stroke rate is responsible for most of the intracycle velocity variation in swimmers with amputations or malformations of the upper limbs. No study was found on propulsive efficiency. Swimmers with disabilities should work more on stroke rate, with small decreases in stroke length to achieve higher swimming speeds, lower swim coordination index (more negative) and lower speed variations.

Reproducibility of an endurance test for master swimmers

Abstract Aim: This study aimed to verify the reproducibility of an endurance test for master swimmers. Methods: Seventeen male swimmers, aged from 30 to 50 years (35.06 ± 5.36), weight (78.71 ± 13.06 kg), height (174.35 ± 0.07 cm), participated in this study. The participants underwent the Progressive Swim Test (PSwT), which corresponds to swimming progressively to exhaustion in a 25-meter pool, guided by the recording of sound signals indicating the swimming rhythm. We verified the following variables: heart rate before the test (BHR) and right after the end of the test (AHR), followed by the rate of perceived exertion (RPE) and a total of laps performed (SN - swimming number). For comparison between the three days of tests, the one-way repeated measures ANOVA with Tukey's Post-Hoc was performed. Relative reproducibility was performed by the r test and the intraclass correlation coefficient (ICC). Absolute reproducibility was determined using the coefficient of variation (CV) and the confidence interval (CI). Results: The results showed high reproducibility in (PSwT) in master swimmers in the analyzed variables of intergroup and intragroup with relative reproducibility of r > 0.60 and ICC > 0.70 and absolute reproducibility with CI 95% (lower −1.10 and upper 0.99) and CV < 10%. Most of the cases were within the limits of agreement of the Bland-Altman tests, all variables were considered a level of significance with (p <0.05). Conclusion: The Progressive Swim Test (PSwT) is a reproducible instrument in the assessment of endurance in master swimmers.

Influence of operator experience, scanner type, and scan size on 3D scans.

STATEMENT OF PROBLEM: Intraoral scanners (IOSs) have some inherent distortions caused by optical and/or software imperfections. However, how other factors such as operator experience, scan time, scanner type, and scan size influence scan accuracy is not clear. PURPOSE: The purpose of this in vitro study was to evaluate the trueness and precision of scans performed by 3 professionals with different levels of experience by using 2 IOSs. MATERIAL AND METHODS: Three operators with low, medium, and high levels of experience scanned a master model 10 times by using 2 IOSs (CEREC Omnicam; Dentsply Sirona and TRIOS 3; 3Shape), resulting in 10 standard tessellation language files for each group (N=60). Each standard tessellation language file was divided into 2 areas (prepared teeth and complete arch). Precision was evaluated by comparing the 10 scans from each examiner for each system. Trueness was evaluated by comparing each scan file with a reference scan obtained from a laboratory scanner (D2000; 3Shape). A 3D analysis software program (Geomagic Control; 3D Systems) was used to perform all the comparisons and superimpositions. The 3-way ANOVA test followed by the Tukey HSD test were used to assess precision and trueness. The 2-way ANOVA followed by the Tukey HSD test was used to assess scan time. The Pearson correlation test was performed between scan time and trueness for both scanners. An additional correlation was performed between scan time and number of images, as well as between number of images and trueness for the TRIOS 3. RESULTS: Statistically significant influences of operator (P<.001), scanner (P<.001), scan size (P<.001), operator and scan size (P<.001), and scanner and scan size (P<.001) were observed. The TRIOS 3 group reported higher precision than the CEREC Omnicam group for complete-arch scans (P<.001), although no difference was observed for scans of the prepared tooth. Medium- (P=.002) and low-experience operators (P<.001) reported lower precision for complete-arch scans performed with CEREC Omnicam when compared with TRIOS 3. The low-experience operator reported significantly worse results for complete-arch scans in comparison with the medium- (P=.008 and P<.001) and high-experience operators (P<.001 and P=.001), by using TRIOS 3 and CEREC Omnicam, respectively. Medium- and high-experience operators reported similar results among themselves. The CEREC Omnicam scanner reported lower trueness for complete-arch scans when compared with the prepared tooth (P<.001); for TRIOS 3, a difference was only observed for the low-experience operator when compared with the high-experience operator (P<.001). The CEREC Omnicam reported lower trueness than the TRIOS 3, except for the medium-experience operator with the prepared tooth scan. Comparing the trueness between operators and considering the same scanner and scan size, all groups were similar. The low-experience operator had a longer scanning time than the medium- and high-experience operators. For TRIOS 3, the low-experience operator obtained the highest number of images during each scan. CONCLUSIONS: The accuracy of intraoral scans was influenced by operator experience, type of IOSs, and scan size. More experienced operators and smaller scan sizes made for more accurate scans. In addition, more experienced operators made faster scans, and the TRIOS 3 was more accurate than the CEREC Omnicam for complete-arch scans.

Neuromuscular and motor patterns in breaststroke technique / Padrões motores e musculares na técnica de bruços

Abstract The aim of this study was to analyze the inter-temporal neuromuscular and motor patterns in breaststroke technique. Five national level male swimmers performed 200 m breaststroke at maximal effort. Electromyography data onbiceps brachii, deltoid anterior, pectoralis major and triceps brachiiwere analysed. The relative duration of active and non-active phase and the average rectified value for the neuromuscular patterns were recorded. The swim bouts were videotaped in sagittal plane with a pair of cameras and the Theme software 5.0 was used toanalyse the detected patterns in each swimmer. The neuromuscular pattern revealed that by the average rectified value the biceps brachiiand triceps brachiiwere increased at the end of the test for swimmers 1 and 5, while biceps brachii. deltoid anteriorand pectoralis majorwere increased for swimmers 2 and 4. Different motor patterns between cycles, and between swimmers were observed.We found similarities between the swimmers, adjusting their style to the technical model. The absence of a neuromuscular pattern for all swimmers could be related to different technical models used by each swimmer, as presented in the motor patterns. These findings suggested that each swimmers adapted their own motor and neuromuscular pattern in a unique and distinct way.

Resumo O objetivo deste estudo consistiu em analisar a relação entre os padrões neuromusculares e motores na técnica do nado de peito. Cinco nadadores masculinos de nível nacional realizaram 200 mnado peito na máxima intensidade.Foram registrados dados de Electromiografia do bicepsbrachii, deltoid anterior, pectoralis major etricepsbrachii. A duração relativa da fase ativa e não ativa e o valor médio retificado dos padrões neuromusculares foram analisados. Os percursos de nado foram gravados no plano sagital e analisados para detectar os padrões motores de cadanadador através do software THÉME software 5.0. Os padrões neuromusculares indicaram, por meio do valor médio retificado que a atividade dos músculos bíceps brachii e tríceps brachii aumentam no final do teste para o nadador 1 e 5, enquanto que o bíceps brachii. deltoid anterior. pectoralis major aumentam para o nadador 2 e 4. Diferentes padrões motores entre ciclos e entre nadadores foram observados, indicando que existem semelhanças entre eles, levando a um ajuste do estilo e modelo técnico de nado. A ausência de um padrão neuromuscular para todos os nadadores poderá estar relacionada com os diferentes modelos técnicos utilizados por cada nadador, como demonstrado nos padrões motores. Esses resultados sugerem que cada nadador adapta o seu padrão motor e muscular de uma forma única e distinta.

Avaliação da higiene oral em prótese fixa múltipla sobre implantes expostos após necrose óssea ­ relato de caso / Evaluation of oral hygiene in multiple fixed prostheses on implants exposed after bone necrosis ­ case report

Alguns pacientes podem apresentar dificuldades motoras que inviabilizam a adequada higienização de próteses implantossuportadas. No entanto, a utilização de escovas elétricas pode compensar essas dificuldades e melhorar a qualidade de higiene oral. O presente trabalho relata um caso clínico que utilizou escova dental elétrica Philips Sonicar e AirFloss Ultra® como meio de higienização da área de implante osseointegrado exposta pós necrose óssea. Paciente dirigiu-se a um serviço de reabilitação oral com desconforto em prótese implantada ferulizada de três elementos localizada no arco inferior direito. Após análise clínica e diagnóstico, o paciente foi orientado quanto as etapas do tratamento, assim como a necessidade da manutenção dos tecidos peri-implantares através de uma correta higienização. Após um ano é possível verificar uma condição satisfatória da região avaliada e maior satisfação do paciente quanto ao método de limpeza empregado. (AU).

Some patients may present motor difficulties that prevent the adequate hygiene of implanted prosthesis. However, the use of electric toothbrushes can compensate these difficulties and improve the quality of oral hygiene. The present study reported the case of a patient who used Philips Sonicar electric brush and AirFloss Ultra® to clean an osseointegrated implant area exposed after bone necrosis. Patient went to an oral rehabilitation service with discomfort in a three-element splinted implanted prosthesis area, located in the lower right arch. After clinical analysis and diagnosis, the patient was advised on the steps of treatment as well as the necessity of the maintenance of the peri-implant tissues with a correct oral hygiene. After one year, it is still possible to verify a satisfactory condition of the evaluated region and greater satisfaction of the patient regarding the cleaning method employed. (AU).

Applicability of an indirect VO2max test: its association with the 400 meters freestyle performance

Abstract - The aim of this study was to evaluate the VO2max using a previously validated indirect test for non-expert adult swimmers and to verify its connection with the 400 meters freestyle test. A total of 17 non-expert male swimmers (21.5 ± 3.12 years) were evaluated. Body composition measurements included body weight (74 ± 9.41 kg), height (172.9 ± 5.21 cm) and body fat percentage (15.2 ± 4.15 %). Two tests were conducted on different days; the 400 meters freestyle (400 MF) and the Progressive Swim Test (PSwT), respectively. The participant's heart rate frequency before and after the test (BHR and AHR) was analyzed, as well as the subjective perception of effort (RPE), the number of laps covered (NLP), and the time of test execution measured in minutes. Significant differences were identified in all variables (p < 0.05) with the exception of BHR. An inverse correlation (r > - 0.60) was found between AHR and execution time (r > - 0.70), as well as between the VO2max estimated by the PSwT and the 400 MF performance test (r > - 0.70). The Bland-Altman Plot showed that the values discovered were within the established concordance limits of 95% (±1.96 SD). A negative correlation between a swimming test and a test that estimates the VO2max occurred, and the PSwT showed results of greater approximation of the aerobic power of non-expert swimmers. In conclusion, the PSwT is applicable for non-expert adult swimmers.

Floristic survey of herbaceous and subshrubby aquatic and palustrine angiosperms of Viruá National Park, Roraima, Brazil.

We provide and discuss a floristic survey of herbaceous and subshrubby aquatic and palustrine angiosperms of Viruá National Park (VNP). The VNP is located in the northern Amazon basin and displays phytophysiognomies distributed in a mosaic where these plants occur, as flooded forests, hydromorphic white-sand savannas, "buritizais" and waterbodies. After expeditions between February/2010 and January/2015 and the analysis of specimens from regional herbaria, we list 207 species of herbaceous and subshrubby aquatic and palustrine angiosperms for the VNP, distributed in 85 genera in 37 families. We recorded six new occurrences for Brazil, two for the northern Brazilian region and 21 for Roraima state. These new occurrences, added to the other species listed here, highlight the floristic similarity between the study site and the Guiana Shield, an adjacent phytogeographical unit and geologically related to the origin of white-sand savannas.

Resumo(Lista Florística de angiospermas herbáceas e subarbustivas aquáticas e palustres do Parque Nacional do Viruá, Roraima, Brasil) Esse estudo foi realizado objetivando a elaboração de uma lista florística das angiospermas herbáceas e subarbustivas aquáticas e palustres do Parque Nacional do Viruá (PNV). O PNV está localizado na região norte da Bacia Amazônica e apresenta fitofisionomias distribuídas em mosaico onde esse grupo de plantas ocorre, como florestas inundáveis, savanas sobre solos de areia branca hidromórficas, buritizais e corpos d'água. Após coletas entre Fevereiro/2010 e Janeiro/2015 e a análise de espécimes depositados em herbários regionais, foram listadas 207 espécies de angiospermas herbáceas e subarbustivas aquáticas e palustres no PNV, distribuídas em 85 gêneros e 37 famílias. Foram registradas seis novas ocorrências para o Brasil, duas para a região norte do Brasil e 21 para o estado de Roraima. Essas novas ocorrências, juntamente com as demais espécies listadas, evidenciam a similaridade florística entre a área de estudo e o Escudo das Guianas, uma unidade fitogeográfica adjacente e geologicamente relacionada com a origem das savanas sobre solos de areia branca.

Microsatellite markers isolated from Cabomba aquatica s.l. (Cabombaceae) from an enriched genomic library.

PREMISE OF THE STUDY: Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters. METHODS AND RESULTS: Using a selective hybridization method, we designed and tested 30 simple sequence repeat loci using two natural populations of C. aquatica s.l., resulting in 13 amplifiable loci. Twelve loci were polymorphic, and alleles per locus ranged from two to four across the 49 C. aquatica s.l. individuals. Observed heterozygosity, expected heterozygosity, and fixation index varied from 0.0 to 1.0, 0.0 to 0.5, and -1.0 to -0.0667, respectively, for the Manaus population and from 0.0 to 1.0, 0.0 to 0.6, and -1.0 to 0.4643 for the Viruá population. CONCLUSIONS: The developed markers will be used in further taxonomic and population studies within Cabomba. This set of microsatellite primers represents the first report on rapid molecular markers in the genus.

Longitudinal intra- and inter-individual variability in young swimmers' performance and determinant competition factors / Análise longitudinal da variabilidade intra- e inter-individual da performance e fatores determinantes em nadadores jovens / Análisis longitudinal de la variabilidade intra- y inter-individual del rendimiento y sus determinantes en nadadores jóvenes

The main purpose of this study was to follow-up the intra- and inter-individual variability of young swimmers' performance and determinant factors over two competitive seasons. Thirty young swimmers (14 boys: 12.33±0.65 years-old; 16 girls: 11.15±0.55 years-old) were followed-up throughout two consecutive seasons (seven evaluation moments). Performance (100m freestyle), anthropometric, kinematic, hydrodynamic and efficiency features were evaluated. A gender and skill-level effect was observed. Boys improved in a higher amount (%) comparing to girls. Overall, swimmers in skill-level 2 (both genders) presented a higher intra-individual variability. Performance and anthropometrics showed a significant inter-individual variability in most moments, but hydrodynamics, kinematics and efficiency did not. Within each skill-level hydrodynamics, kinematics and efficiency were the variables that showed a high inter-individual variability. As a gender and skill-level effect was noticed in an age-group of young swimmers, coaches and practitioners should put the focus in specific and customized training plans for each skill-level of swimmers...

"Análise longitudinal da variabilidade intra- e inter-individual da performance e fatores determinantes em nadadores jovens." O objetivo principal do estudo foi acompanhar a variabilidade intra- e inter-individual da performance e dos seus fatores determinantes de nadadores jovens durante duas temporadas competitivas. Trinta nadadores jovens (14 nadadores: 12.33±0.65 anos; 16 nadadoras: 11.15±0.55 anos) foram avaliados ao longo das duas épocas (7 momentos de avaliação). Foi avaliada a performance (100m livres), e variáveis antropométricas, cinemáticas, hidrodinâmicas e de eficiência. Foi observado um efeito do sexo e do nível competitivo. Os nadadores melhoraram em maior quantidade (%) do que as nadadoras. Os nadadores no nível competitivo 2 (ambos os sexos) apresentaram uma intra-variabilidade superior. A performance e as variáveis antropométricas apresentaram uma inter-variabilidade significativa na maior parte dos momentos de avaliação, ao contrário das variáveis hidrodinâmicas, cinemáticas e de eficiência. Dentro de cada nível competitivo as variáveis hidrodinâmicas, cinemáticas e de eficiência foram as que apresentaram uma variabilidade inter-individual superior. Como foi verificado um efeito do sexo e do nível competitivo, treinadores e praticantes devem colocar o foco em treinos específicos e personalizados para cada grupo de nadadores de acordo com o nível competitivo...

"Análisis longitudinal de la variabilidade intra- y inter-individual del rendimiento y sus determinantes en nadadores jóvenes." El objetivo principal del estudio fue el seguimiento de la variabilidad intra- e inter-individual del rendimiento y sus factores determinantes de nadadores jóvenes en dos temporadas competitivas. Treinta nadadores jóvenes (14 niños: 12.33±0.65 años; 16 niñas: 11.15±0.55 años) fueron seguidos a lo largo de dos temporadas competitivas (7 momentos de evaluación). Se evaluó el rendimiento (100m estilo libre), e características antropométricas, cinemáticas, hidrodinámicas e la eficiencia. Se observó un efecto del sexo e del nivel de habilidad. Los niños mejoraron en una cantidad superior (%) en comparación con las niñas. En general, los nadadores de nivel de habilidad 2 (los dos sexos) presentaron una mayor variabilidad intra-individual. El rendimiento y la antropometría mostraron una variabilidad inter-individual significativa en la mayoría de los momentos, lo mismo no ocurrió con la hidrodinámica, la cinemática y la eficiencia. Dentro de cada nivel de habilidad, la hidrodinámica, la cinemática y la eficiencia fueron las variables que mostraron una alta variabilidad inter-individual. Como se ha observado un efecto de sexo e nivel de habilidad, entrenadores y practicantes deben poner el foco en los planes de formación específicos y personalizados para cada nivel de habilidad de los nadadores...

Inclusão do equivalente do lactato sanguíneo O2 na regressão de intensidade de exercício VO2 aumenta o gasto energético de corrida e diminui sua precisão / Inclusion of blood lactate O2 equivalent in the VO2 exercise intensity regression increases the energy cost of running and lowers its precision / La inclusión del equivalente del lactato sanguíneo O2 en la regresión de intensidad de ejercicio VO2 aumenta el gasto energético de la carrera y disminuye su precisión

INTRODUÇÃO E OBJETIVOS: Investigar se a inclusão do equivalente energético de lactato sanguíneo (LS) em intensidades submáximas alteraria o gasto energético de corrida (Gc) estimado para corrida supramáxima de alta intensidade assim como sua precisão MÉTODOS: O Gc foi determinado a partir da curva de regressão de velocidade do VO2. Para cada sujeito, uma regressão alternativa foi estabelecida em que energia equivalente a 3 ml de O2 kg-1∙mM-1 de LS foi adicionada ao VO2. Testes t pareados, correlações pareadas e plotagem de Bland-Altman foram utilizados para investigar se os dois métodos representam os mesmos fenômenos. RESULTADOS: Os principais achados foram que o Gc previsto para intensidades supramáximas incluídos entre 110 e 160% ao correspondente ao pico de VO2 dos indivíduos aumentou significativamente quando as medidas de LS foram incluídas nas regressões. A inclusão das medidas de LS também aumentaram a imprecisão do Gc a 110, 120, 130, 150 e 160%. CONCLUSÃO: Nossos resultados indicam que a inclusão do equivalente do lactato de O2 na regressão de intensidade de VO2-exercício aumenta o gasto energético e diminui sua precisão.

INTRODUCTION AND OBJECTIVES: Investigate whether the inclusion of the blood lactate (BL) energy equivalent at submaximal intensities would change the energy cost of running (CR) predicted to supramaximal high-intensity running as well as its precision. METHODS: The CR was determined from the slope of the VO2-speed regression. For each subject an alternative regression was established where an energy equivalent of 3 ml O2 kg-1∙mM-1 BL was added to the VO2. Paired t-tests, pairwise correlations and Bland-Altman plots were used to investigate whether the two methods represent the same phenomena. RESULTS: The main findings were that CR predicted to supramaximal intensities comprised between 110% and 160% of that corresponding to the individuals' peak VO2, increased significantly when the BL measurements were included in the regressions. The inclusion of BL measurements also increased the imprecision of the CR at 110%, 120%, 130%, 150% and 160%. CONCLUSIONS: Our results indicate that inclusion of lactate O2 equivalent in the VO2-exercise intensity regression increases the energy cost of running and lowers its precision.

INTRODUCCIÓN Y OBJETIVOS: Investigar si la inclusión del equivalente energético de lactato sanguíneo (LS), en intensidades submáximas, modificaría el gasto energético de la carrera (Gc), estimado para carrera supramáxima de alta intensidad, así como su precisión. MÉTODOS: El Gc fue determinado a partir de la curva de regresión de velocidad del VO2. Para cada individuo, se estableció una regresión alternativa en la cual energía equivalente a 3 ml de O2 kg-1∙mM-1 de LS fue adicionada al VO2. Pruebas t por pares, correlaciones por pares y plotaje de Bland-Altman fueron utilizados para investigar si los dos métodos representan los mismos fenómenos. RESULTADOS: Los hallazgos principales fueron que el Gc previsto para intensidades supramáximas, incluidos 110% y 160% de lo que corresponde al pico de VO2 de los individuos, aumentó significativamente cuando las medidas de LS fueron incluidas en las regresiones. La inclusión de las medidas de LS también aumentó la imprecisión del Gc a 110%, 120%, 130%, 150% y 160%. CONCLUSIÓN: Nuestros resultados indican que la inclusión del equivalente del lactato de O2, en la regresión de intensidad de VO2-ejercicio, aumenta el gasto energético y disminuye su precisión.

Observação e caracterização técnica em natação pura desportiva: 200 m bruços / Observation and technical characterization in swimming: 200 m breaststroke

OBJETIVO: Caracterização da técnica de bruços, na relação entre os parâmetros cinemáticos e neuromusculares. MÉTODO: A eletromiografia de superfície (EMG) foi utilizada para analisar a dinâmica da atividade neuromuscular nos músculos peitoral maior (PM), bicípite braquial (BB), tricípite braquial (TB) e deltoide anterior (DA) em 12 nadadores de elite nacional. Foi utilizado um par de câmaras, fornecendo uma projeção dupla a partir de uma câmara subaquática e outra acima da superfície da água, para análise das variáveis cinemáticas: velocidade de nado (VN), frequência gestual (FG) e distância de ciclo (DC) em 200m bruços. RESULTADOS: A VN diminui de 1,41 (0,07) para 1,16 (0,09)m.s-1 (P < 0,05). A DC diminuiu de 2,32 (0,37) para 1,96 (0,24)m, enquanto a FG sofreu um decréscimo de 37,52 (5,16) para 34,40 (3,58) ciclo/min do primeiro percurso de 50m até ao terceiro percurso de 50 m, aumentando ligeiramente no último percurso para 35,82 (3,39) ciclo/min. O lactato sanguíneo aumentou de repouso para o pico de lactato sanguíneo de 1,12 (0,22) para 12,00 (3,23) mmol.L-1. Os resultados de EMG indicaram um aumento da frequência em relação à amplitude para todos os músculos em estudo, exceto para o DA. Correlações negativas foram obtidas entre a frequência e a VN, FG e DC, ou seja, para os músculos BB, TB e PM, verificou-se uma correlação forte entre VN, FG e DC, isto é, à medida que as variáveis cinemáticas aumentam a frequência diminui. As correlações sugerem que a ativação neuromuscular apresenta relação direta com as variáveis cinemáticas, nomeadamente para uma diminuição da frequência, nos músculos BB, TB e PM e para uma elevada amplitude e forte correlação com as variáveis cinemáticas em PM. CONCLUSÃO: A relação entre as variáveis cinemáticas e EMG são determinantes na avaliação da performance em natação pura desportiva, nomeadamente, no suporte na prescrição de exercícios para o aumento da resistência muscular dos músculos envolvidos na técnica de bruços.

INTRODUCTION: Characterization of the breaststroke technique, regarding the relationship between kinematic and neuromuscular parameters. METHOD: Surface electromyographic signals (EMG) were used to analyze the dynamics of neuromuscular activity of the muscles pectoralis major (PM), biceps brachii (BB), triceps brachii (TB) and anterior deltoid (AD), in twelve national elite swimmers. A couple of cameras (an underwater camera and an above the water surface camera) were used to provide a dual projection that permits analysis of kinematic variables (Speed, SF, SL) in the 200 m breaststroke event. RESULTS: Swimming speed decreased from 1.41 (0.07) to 1.16 (0.09) m.s-1(P<0.05). Stroke length decreased from 2.32 (0.37) to 1.96 (0.24) m, while stroke frequency suffered decrease from 37.52 (5.16) to 34.40 (3.58) cycle/min of 1st lap 50 m until the 3rd lap of 50 m, slightly increasing in the last lap to 35.82 (3.39) cycle/min. Blood lactate increased from 1.12 (0.22) to 12.00 (3.23) mmol.L-1. EMG results indicated increase in frequency concerning amplitude for all muscles studied: BB, PM and TB, except for the AD. Negative correlation between speed frequency, SF and SL was obtained, i.e. to the muscles BB, TB and PM there was a correlation between speed, SF and SL, meaning that as the kinematic variables increase, the frequency decreases. The correlations suggested that the neuromuscular activation presents a direct correlation with the kinematic variables, especially for frequency reduction in the BB, TB and PM muscles, and to a high extent and correlation with the kinematic variables in PM. CONCLUSION: The relationship between the kinematic variables and EMG is decisive in the swimming performance evaluation, in training exercises outside the pool to increase muscular endurance of muscles involved in the breaststroke technique.

Análise dos parâmetros cinemáticos determinantes do desempenho na prova de 200 m nado livre / Analysis of the determinant kinematical parameters for performance in the 200-m freestyle swimming event

Os objetivos deste estudo foram: i) caracterizar o nado submáximo e máximo do ponto de vista cinemático; ii) verificar a influência das variáveis cinemáticas na prova máxima de 200 metros crawl. Nove nadadores de elite nacionais realizaram dois testes: um submáximo, descontínuo de intensidade progressiva; outro máximo, que consistiu em uma simulação de uma prova de 200 m crawl. Foram estudados os parâmetros cinemáticos gerais da mecânica da braçada, a duração de cada fase do ciclo gestual, a velocidade do centro de massa e a variação intracíclica da velocidade horizontal. Verificaram-se diferenças cinemáticas significativas da frequência gestual, velocidade de deslocamento do centro de massa, duração total do ciclo gestual, duração da ação subaquática propulsora e da velocidade do centro de massa na fase de recuperação entre os testes. Identificou-se associações significativas entre o teste máximo e algumas variáveis cinemáticas como a velocidade do centro de massa e índice de nado.

The main aims of this study were i) to perform a kinematic characterization of sub and maximal swimming speed; ii) to investigate the associations between the kinematic profiles and the 200 m front crawl style. Nine national level male swimmers performed two speed tests: a submaximal discontinuous speed trial of progressive speed intensity, and a maximal one, simulating a 200 m front crawl style. The stroke general kinematic parameters, the phases duration of the stroke cycle, the velocity of the centre of mass and the intra cyclic variation of the horizontal velocity were herein studied. Significant kinematic differences in relation to stroke rate, horizontal velocity of the centre of mass, total cycle duration, absolute and relative duration of the propulsive sub aquatic action and the velocity of the centre of mass during the arms recovery were compared between the speed tests. Significant correlations between the performance in the maximal speed and some kinematic variables were observed.

Stingless bees damage broccoli inflorescences when collecting fibers for nest building

The stingless bee Trigona spinipes (Fabricius, 1793) (Hymenoptera: Apidae) is an important pollinator for various crops, but constitutes an occasional pest of other plant species since it causes injury to leaves, stems, flowers and fruits while collecting nest materials. The aim of the present study was to determine the damage caused by T. spinipes to a broccoli (Brassica oleracea L. var. italica, Brassicaceae) growing on an organic farm. A significant number of plants (72.5 %) presented damaged inflorescences, while 39% of all of the inflorescences suffered some degree of injury. The activities of T. spinipes caused scarifications on the stems of the inflorescences, and these typically evolved to epidermal cicatrices up to 10 mm wide. In some cases, the lesions were sufficiently deep to cause partial destruction of the vascular tissues, and this lead to thinner ( 5 mm diameter) floral stems that may collapse. To the best of our knowledge, this is the first report concerning the attack of broccoli plants by T. spinipes. The results obtained should serve to highlight the possibility that stingless bees could be responsible for direct and/or indirect damage to vegetable crops, and to stimulate the development of control strategies for these incidental pests.