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1.
Transbound Emerg Dis ; 62(4): 407-10, 2015 Aug.
Article in English | MEDLINE | ID: mdl-23931548

ABSTRACT

Bovine respiratory syncytial virus (BRSV) plays a significant role in the etiopathogenesis of the respiratory syndrome in young cattle during their first year of life. Development of rapid and accurate BRSV diagnostic tools would aid in the appropriate control of this important pathogen. The objective of this study was to characterize infections induced by BRSV by means of rapid patient-side immunomigration assays used for diagnosis of human respiratory syncytial virus (hRSV) in humans. Nasal and tracheal swabs were obtained from healthy calves of various beef and dairy breeds - Holstein-Friesian, Simmental, Charolais, Belgian Blue and Limousin, between the ages of 5 and 12 months, from 26 farms. BRSV was identified using two rapid immunomigration assays, TruRSV® and Clearview® RSV, and compared with RT-PCR as a reference technique. BRSV was found in 73.1% of all the herds tested. High agreement with RT-PCR was obtained for TruRSV® (κ = 0.824), while in the case of the Clearview® RSV test, agreement with PCR was moderate (κ = 0.420). The results demonstrate that rapid patient-side immunomigration assays designed to detect hRSV can be used to accurately detect BRSV in field samples collected from cattle.


Subject(s)
Cattle Diseases/diagnosis , Immunoassay/methods , Respiratory Syncytial Virus Infections/veterinary , Respiratory Syncytial Virus, Bovine/isolation & purification , Animals , Antigens, Viral/classification , Antigens, Viral/isolation & purification , Cattle , Cattle Diseases/immunology , Cattle Diseases/virology , Nasal Mucosa/virology , Poland , Polymerase Chain Reaction , Reagent Kits, Diagnostic , Respiratory Syncytial Virus Infections/diagnosis , Respiratory Syncytial Virus Infections/immunology , Respiratory Syncytial Virus, Bovine/immunology , Trachea/virology
2.
Microb Pathog ; 45(4): 241-57, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18586081

ABSTRACT

We hypothesized that particular genetic backgrounds enhance rates of colonization, increase severity of enteritis, and allow for extraintestinal spread when inbred IL-10(-/-) mice are infected with pathogenic C. jejuni. Campylobacter jejuni stably colonized C57BL/6 and NOD mice, while congenic strains lacking IL-10 developed typhlocolitis following colonization that mimicked human campylobacteriosis. However, IL-10 deficiency alone was not necessary for the presence of C. jejuni in extraintestinal sites. C3H/HeJ tlr4(-/-) mice that specifically express the Cdcs1 allele showed colonization and limited extraintestinal spread without enteritis implicating this interval in the clinical presentation of C. jejuni infection. Furthermore, when the IL-10 gene is inactivated as in C3Bir tlr4(-/-) IL-10(-/-) mice, enteritis and intensive extraintestinal spread were observed, suggesting that clinical presentations of C. jejuni infection are controlled by a complex interplay of factors. These data demonstrate that lack of IL-10 had a greater effect on C. jejuni induced colitis than other immune elements such as TLR4 (C3H/HeJ, C3Bir IL-10(-/-)), MHC H-2g7, diabetogenic genes, and CTLA-4 (NOD) and that host genetic background is in part responsible for disease phenotype. C3Bir IL-10(-/-) mice where Cdcs1 impairs gut barrier function provide a new murine model of C. jejuni and can serve as surrogates for immunocompromised patients with extraintestinal spread.


Subject(s)
Campylobacter Infections/genetics , Campylobacter jejuni/physiology , Enteritis/microbiology , Host-Pathogen Interactions , Interleukin-10/immunology , Animals , Antibodies, Bacterial/blood , Campylobacter Infections/immunology , Campylobacter Infections/microbiology , Campylobacter Infections/pathology , Campylobacter jejuni/immunology , Campylobacter jejuni/pathogenicity , Enteritis/genetics , Enteritis/immunology , Enteritis/pathology , Humans , Interleukin-10/genetics , Mice , Mice, Inbred Strains , Mice, Knockout , Phenotype
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