Robustness of focused and global impedance estimates of bladder volumes against uncertainty of urine conductivity.

Bioimpedance measurements are currently used to monitor various biological processes and are potentially useful for studies of urodynamics. Global impedance (GI) and focused impedance measurements (FIM) can be used to monitor bladder volumes, but these are subject to varying conductivity of urine. To address this, we emulated a human bladder using an agar phantom filled with saline solutions of varying conductivities and estimated volumes using a modified FIM-based approach. Using this novel strategy, electrical potentials did not change significantly with constant liquid volumes, even when the conductivity of the saline solutions was varied between 1.027 to 1.877 and 2.610 S/m. Conversely, GI and classic FIM measurements of constant liquid volumes varied with conductivity. These observations suggest that the proposed FIM approach is suitable for bladder volume estimation due to its robustness against uncertainties of conductivity. The bioimpedance hardware used in our experiments comprised 8 electrodes and a a small and low cost impedance measurement system based on an AFE4300 direct impedance measurement device.

Impedance Alterations in Healthy and Diseased Mice During Electrically Induced Muscle Contraction.

Alterations in the health of muscles can be evaluated through the use of electrical impedance myography (EIM). To date, however, nearly all work in this field has relied upon the measurement of muscle at rest. To provide an insight into the contractile mechanisms of healthy and disease muscle, we evaluated the alterations in the spectroscopic impedance behavior of muscle during the active process of muscle contraction. The gastrocnemii from a total of 13 mice were studied (five wild type, four muscular dystrophy animals, and four amyotrophic lateral sclerosis animals). Muscle contraction was induced via monophasic current pulse stimulation of the sciatic nerve. Simultaneously, multisine EIM (1 kHz to 1 MHz) and force measurements of the muscle were performed. Stimulation was applied at three different rates to produce mild, moderate, and strong contractions. We identified changes in both single and multifrequency data, as assessed by the Cole impedance model parameters. The processes of contraction and relaxation were clearly identified in the impedance spectra and quantified via derivative plots. Reductions in the center frequency fc were observed during the contraction consistent with the increasing muscle fiber diameter. Different EIM stimulation rate-dependencies were also detected across the three groups of animals.