ABSTRACT
The development of experimental cholera in suckling rabbits is associated with typical cholerogenic syndrome: the presence of Vibrio cholerae in the blood, bile (in 60 and 70% cases, respectively), small and large intestine (in 100% cases). Simultaneously with enterocyte desquamation and increased permeability of the blood-enterocyte barrier, the vibrios are released into villous stroma and then into the microcirculatory bed. the zot toxin is involved in the mechanism of Vibrio cholerae invasion, the corresponding gene is present in the genome of the studied strain.
Subject(s)
Cholera/metabolism , Intestine, Small , Vibrio cholerae/metabolism , Animals , Animals, Newborn , Animals, Suckling , Cholera/microbiology , Cholera Toxin/metabolism , Endotoxins , Erythrocytes/microbiology , Erythrocytes/ultrastructure , Intestine, Small/metabolism , Intestine, Small/pathology , Intestine, Small/ultrastructure , Rabbits , Vibrio cholerae/ultrastructureABSTRACT
Investigations on experimental models of cholera ("sealed" mice and suckling rabbits) demonstrated that previous daily oral administration of the ferment culture of Lactobacillus acidophilus BKM B-2020[symbol: see text] in a dose of 3.0 x 10(8) microbial cells/ml daily for 5-7 days prevented to the development of Vibrio cholerae infection. The curative effect observed after 3 administrations of lactobacilli within 48 hours after infection with V. cholerae was registered in 50% of cases. This strain of lactobacilli was found to be suitable for use as the basis component of probiotic, an additional remedy for the prophylaxis and treatment of cholera.
Subject(s)
Cholera/therapy , Lactobacillus acidophilus , Probiotics/therapeutic use , Animals , Animals, Suckling , Cholera/prevention & control , Mice , RabbitsABSTRACT
The study revealed the possibility, on principle, for L. acidophilus strain VKM V-2020 D to colonize the intestine of white mice with the preservation of the viability of lactobacilli subjected to the action of antibiotics. The culture of this strain, isolated from the animals, showed the stability of its biological properties: resistance to polymyxin M, kanamycin, cyprofloxacin, nalidixic acid (including acquired resistance to rifampicin), as well as pronounced antagonism with respect to Vibrio cholerae. Good prospects for the use of L. acidophilus strain VKM V-2020 D for further studies regarding its use for prophylaxis and therapy were noted.
Subject(s)
Anti-Bacterial Agents/therapeutic use , Intestines/microbiology , Lactobacillus acidophilus/drug effects , Animals , Antibiosis , Drug Resistance, Microbial , Drug Resistance, Multiple , Feces/microbiology , Lactobacillus acidophilus/isolation & purification , Mice , Time Factors , Vibrio choleraeABSTRACT
The virulence of V. cholerae isolated on the territory of Russia and other CIS countries from environmental objects and from humans in different epidemic situations during the period of 1987-1991 was studied. The analysis of the data obtained in this study revealed that intensive epidemic complications were linked with the realization of the pathogenic properties of strains, characterized by the presence of the ctx gene and the absence of hemolytic activity. As a rule, in single cases or in isolated group cases of cholera V. cholerae hemolysin-negative strains without the cholera toxin gene were isolated.
