ABSTRACT
We propose a model for a description of dynamics of cracklike processes that occur at the interface between two blocks prior to the onset of frictional motion. We find that the onset of sliding is preceded by well-defined detachment fronts initiated at the slider trailing edge and extended across the slider over limited lengths smaller than the overall length of the slider. Three different types of detachment fronts may play a role in the onset of sliding: (i) Rayleigh (surface sound) fronts, (ii) slow detachment fronts, and (iii) fast fronts. The important consequence of the precursor dynamics is that before the transition to overall sliding occurs, the initially uniform, unstressed slider is already transformed into a highly nonuniform, stressed state. Our model allows us to explain experimental observations and predicts the effect of material properties on the dynamics of the transition to sliding.
ABSTRACT
Among the glutamate-requiring strains of Schizosaccharomyces pombe previously described [1], glu2 and glu3 strains were both shown to lack NAD-specific isocitrate dehydrogenase. glu4 strains were shown to lack glutamine:2-oxoglutarate aminotransferase (GOGAT), and to be defective in ammonia assimilation. The regulation of GOGAT activity in wild-type cells was investigated and was consistent with GOGAT and glutamine synthetase being involved in ammonium assimilation, particularly under conditions of nitrogen limitation.
Subject(s)
Glutamates/metabolism , Schizosaccharomyces/enzymology , Gene Expression Regulation, Enzymologic , Gene Expression Regulation, Fungal , Glutamate Synthase/genetics , Glutamic Acid , Isocitrate Dehydrogenase/genetics , Mutation/physiology , Schizosaccharomyces/genetics , Schizosaccharomyces/growth & developmentABSTRACT
From a gene bank of S. pombe DNA, a 5.6 kb clone was isolated which complemented mutants defective in glutamine synthetase (GS) activity. Sub-cloning fragments of this 5.6 kb clone showed that the complementing activity was localised in a 1.6 kb HindIII-AvaI fragment and a partial DNA sequence revealed an open reading frame preceded by TATA sequences and a TGACTA sequence. Plasmid constructs carrying up to 3.4 kb of DNA used to transform gln- strains gave transformants which showed a wide range of GS activity, in some cases 100 times the wild-type level. These constructs identify DNA sequences lying downstream from the putative coding sequence which have effects on the total amount of enzyme activity, but do not affect the control imposed by the nitrogen source on which the cells are grown.
