Fecal glucocorticoid metabolites and ectoparasites as biomarkers of heat stress close to roads in a Mediterranean lizard.

Differences between air and ground temperatures are expected to narrow with the advance of the season in temperate regions (aka seasonal restriction in the availability of thermal microhabitats), which may activate behavioral and physiological responses of ectotherm species adapted to temperate climates. However, according to cost-benefit models of ectotherm thermoregulation, we hypothesize that these responses may also carry some costs. We quantified seasonal shifts in thermoregulatory precision, concentration of fecal glucocorticoid metabolites, and load of ectoparasites in a Mediterranean lizard, Psammodromus algirus. We also tested whether the proximity to a road, a putative source of chronic stress, can facilitate the glucocorticoid-mediated response of lizards to heat stress. As expected, differences between body and environmental temperatures narrowed during the reproductive season and lizards responded by increasing their thermoregulatory precision and the secretion of glucocorticoids, as indicated by metabolites in feces. Interestingly, lizards tended to have higher glucocorticoid concentration when captured far from the road. This might reflect either a putative impairment of the glucocorticoid-mediated response of the lizards to heat stress close to the road or the plastic capability of P. algirus to acclimate to sources of moderate chronic stress. In the latter direction, the increase of both glucocorticoid metabolites and thermoregulatory precision supported that this Mediterranean species responds to environmental thermal restrictions with adaptive behavioral and physiological mechanisms. However, this was also associated with an increase in its susceptibility to ectoparasites, which represents an added cost to the current cost-benefit models of ectotherm thermoregulation.

The landscape of fear: Why some free-ranging rodents choose repeated live-trapping over predation risk and how it is associated with the physiological stress response.

Live trapping is an essential element of field ecological studies. However, the act of trapping provides two types of conditional benefits (food from the bait when hungry, and refuge from a predator when threatened) against one type of drawback (confinement). Our understanding of how animals assess the two benefits against the lone risk determines how we interpret classic field studies in chemical ecology and wildlife management. Here, we studied wood mice responses to these risks and rewards of field trapping by examining experience through recapture and faecal corticosterone metabolites (FCM) as a physiological response indicator. Wood mice were live-trapped in two different plots subjected to two distinct phases: phase 1, absence of predator cues, and phase 2, in which traps were treated with red fox faeces. During phase 1, the recapture percentage was lower indicating that mice avoided traps while FCM levels in recaptured mice were higher. On the contrary, during phase 2, despite the total number of captures was lower we found an increase in the recapture percentage and FCM levels did not increase in recaptured mice. Our results suggest that under increased risk perception traps could be likely considered as a suitable shelter and thus, for some individuals the benefits of traps may outweigh their risks. In addition, we discovered that the effects of combining two stressors do not result in the addition of the response originated by each factor separately.

Quantification of sexual steroid hormones in faeces of Iberian wolf (Canis lupus signatus): a non-invasive sex typing method.

The determination of gender in wild animals is essential for behavioural and ecological studies, and also for conservation. The objectives of this study were (i) the determination of gender in faecal samples of Iberian wolf based on the differential concentrations of sexual steroid hormones (SSH) and (ii) to analyse the profiles of SSH in males and females (considering the gender determination carried out previously) during the non-reproductive and reproductive periods. The quantification of androgens (testosterone, T), progestin (progesterone, P) and oestrogen (oestradiol, E) was conducted by means of enzyme immunoassay. The k-means conglomerate analysis showed that the 59 faecal samples grouped into three different conglomerates, considering SSH levels. Groups 1 and 2 showed higher levels of T than group 3. Therefore, the faecal samples included in groups 1 and 2 (17 samples) corresponded to males and those of group 3 (42 samples) to females. The levels of T + P + E and T/P were higher in the group of males than in the group of females. The results of this study also showed that levels of T in males were higher during the reproductive period than in the non-reproductive period. However, the concentrations of P and E turned out to be higher during the non-reproductive season. In females, the levels of the three hormones (T, P and E) were higher during the reproductive period.