ABSTRACT
The effect of gamma-radiation doses ranging between 10 and 180 Gy on 4-6-day-old adult males of Glossina tachinoides, Glossina fuscipes fuscipes and Glossina brevipalpis was studied. Fecundity of their mates was reduced by 95% following exposure to 120, 80-100 and 50 Gy of adult male G. tachinoides, G. f. fuscipes and G. brevipalpis respectively. Insemination ability of the males and sperm motility were not adversely affected by the radiation treatment. The higher proportion of dominant lethals in the sperm of the three species with increasing radiation doses was reflected in the reproductive status of the female mates, i.e. an increasing percentage of females showing imbalances between intrauterine content and ovarian development (females with an empty uterus due to expulsion of a dead embryo after embryonic arrest or a degenerating egg in utero) and an acceleration in follicle development associated with successive unsuccessful cycles. In the F1 progeny of all treated groups, no significant bias of the sex ratio was found. The average life span of G. tachinoides and G. f. fuscipes males treated with doses of > or = 80 Gy and of G. brevipalpis males treated with doses >140 Gy was significantly reduced as compared with untreated controls. Male G. brevipalpis treated with doses ranging between 10 and 40 Gy, however, showed a significant radiation induced increase in average life span.
Subject(s)
Gamma Rays , Glossinidae/radiation effects , Radiation Tolerance , Animals , Female , Fertility/radiation effects , Glossinidae/physiology , Male , Reproduction/radiation effects , Species SpecificityABSTRACT
Comparison of the survival, fecundity and offspring size of Glossina palpalis palpalis females fed reconstituted oven-dried blood, fresh, frozen/thawed, or reconstituted freeze-dried blood showed that oven-drying at 45 degrees C does not diminish the nutritional quality of blood. The significance of this finding is discussed with a view to optimizing costs and conditions of blood-diet storage and transportation in the context of mass-rearing of tsetse flies.
