ABSTRACT
A chromatographic method using ultra performance LC with ultraviolet spectrophotometry detection system (UPLC-UV-visible (UV/Vis)) (UPLC, ultra performance LC) was developed to determine Chimassorb 944 and Tinuvin 770, high- and low-molecular mass hindered amine light stabilizers, respectively. The use of a bridge ethylene hybrid-based particles C(18) UPLC column, being compatible with a buffered mobile phase adjusted to pH 11.5, was used to achieve a satisfactory elution of possible non-ionizated units of polymeric Chimassorb 944. In addition, a HPLC with positive ion mode ESI and hybrid linear ion trap (LTQ) Fourier transform (FT) Orbitrap MS detection system (HPLC-UV/Vis-ESI-LTQ(FT)Orbitrap) was used for screening and identification purposes. Reliable determinations of monomeric units of Chimassorb 944 and molecule of Tinuvin 770 were achieved by HPLC-ESI(+)-LTQ FT Orbitrap MS method using scan mode. Quality parameters of UPLC-UV/Vis method were evaluated. Obtained LODs and LOQs were 3.41 and 11.4 mg/L for Tinuvin 770 and 0.83 and 2.78 mg/L for Chimassorb 944, respectively. Satisfactory quality parameters of this chromatography method provided the chance to count on a good tool to know the compliance with legal requirements.
ABSTRACT
An analytical method for the simultaneous separation and quantitation of ten colorants and six antioxidants used as polymer additives has been developed by transferring chromatographic conditions from two independent HPLC methods to a single ultraperformance liquid chromatography system. An experimental design was carried out to achieve the best elution gradient for separation of all the studied compounds with the best possible peak resolution and in the shortest possible analysis time. The new method gives good values for the analytical quality parameters evaluated.
