Low-Intensity Physical Exercise Decreases Inflammation and Joint Damage in the Preclinical Phase of a Rheumatoid Arthritis Murine Model.

Lifestyle modifications in preclinical Rheumatoid Arthritis (RA) could delay the ongoing pathogenic immune processes and potentially prevent its onset. Physical exercise (PE) benefits RA patients; however, its impact in reducing the risk of developing RA has scarcely been studied. The objective was to describe the effects of low-intensity PE applied at the disease's preclinical phase on the joints of DBA/1 mice with collagen-induced arthritis (CIA). Twelve mice with CIA were randomly distributed into two groups: the CIA-Ex group, which undertook treadmill PE, and the CIA-NoEx, which was not exercised. The effects of PE were evaluated through clinical, histological, transcriptomics, and immunodetection analyses in the mice's hind paws. The CIA-Ex group showed lower joint inflammation and damage and a decreased expression of RA-related genes (Tnf Il2, Il10, Il12a, IL23a, and Tgfb1) and signaling pathways (Cytokines, Chemokines, JAK-STAT, MAPK, NF-kappa B, TNF, and TGF-beta). TNF-α expression was decreased by PE in the inflamed joints. Low-intensity PE in pre-arthritic CIA reduced the severity through joint down-expression of proinflammatory genes and proteins. Knowledge on the underlying mechanisms of PE in preclinical arthritis and its impact on reducing the risk of developing RA is still needed.

Complete Freund's Adjuvant Induces a Fibroblast-like Synoviocytes (FLS) Metabolic and Migratory Phenotype in Resident Fibroblasts of the Inoculated Footpad at the Earliest Stage of Adjuvant-Induced Arthritis.

The fibroblast-like synoviocytes (FLS) have a crucial role in the pathogenesis of Rheumatoid Arthritis (RA); however, its precise mechanisms remain partially unknown. The involvement of the fibroblast in activating adjuvant-induced arthritis (AA) has not been previously reported. The objective was to describe the participation of footpads' fibroblasts in the critical initial process that drives the AA onset. Wistar rats were injected with Complete Freund's Adjuvant (CFA) or saline solution in the hind paws' footpads and euthanized at 24 or 48 h for genetic and histological analyses. Microarrays revealed the differentially expressed genes between the groups. The CFA dysregulated RA-linked biological processes at both times. Genes of MAPK, Jak-STAT, HIF, PI3K-Akt, TLR, TNF, and NF-κB signaling pathways were altered 24 h before the arrival of immune cells (CD4, CD8, and CD68). Key markers TNF-α, IL-1ß, IL-6, NFκB, MEK-1, JAK3, Enolase, and VEGF were immunodetected in fibroblast in CFA-injected footpads at 24 h but not in the control group. Moreover, fibroblasts in the CFA inoculation site overexpressed cadherin-11, which is linked to the migration and invasion ability of RA-FLS. Our study shows that CFA induced a pathological phenotype in the fibroblast of the inoculation site at very early AA stages from 24 h, suggesting a prominent role in arthritis activation processes.

Levofloxacin induces differential effects in the transcriptome between the gut, peripheral and axial joints in the Spondyloarthritis DBA/1 mice: Improvement of intestinal dysbiosis and the overall inflammatory process.

To analyze the effect of levofloxacin-induced intestinal microbiota modifications on intestinal, joint, and systemic inflammation in the DBA/1 mice with spontaneous arthritis. The study included two groups of mice, one of which received levofloxacin. The composition and structure of the microbiota were determined in the mice's stool using 16S rRNA sequencing; the differential taxa and metabolic pathway between mice treated with levofloxacin and control mice were also defied. The effect of levofloxacin was evaluated in the intestines, hind paws, and spines of mice through DNA microarray transcriptome and histopathological analyses; systemic inflammation was measured by flow cytometry. Levofloxacin decreased the pro-inflammatory bacteria, including Prevotellaceae, Odoribacter, and Blautia, and increased the anti-inflammatory Muribaculaceae in mice's stool. Histological analysis confirmed the intestinal inflammation in control mice, while in levofloxacin-treated mice, inflammation was reduced; in the hind paws and spines, levofloxacin also decreased the inflammation. Microarray showed the downregulation of genes and signaling pathways relevant in spondyloarthritis, including several cytokines and chemokines. Levofloxacin-treated mice showed differential transcriptomic profiles between peripheral and axial joints and intestines. Levofloxacin decreased the expression of TNF-α, IL-23a, and JAK3 in the three tissues, but IL-17 behaved differently in the intestine and the joints. Serum TNF-α was also reduced in levofloxacin-treated mice. Our results suggest that the microbiota modification aimed at reducing pro-inflammatory and increasing anti-inflammatory bacteria could potentially be a coadjuvant in treating inflammatory arthropathies.

Damage Localization in Composite Plates Using Wavelet Transform and 2-D Convolutional Neural Networks.

Nondestructive evaluation of carbon fiber reinforced material structures has received special attention in the last decades. Usage of Ultrasonic Guided Waves (UGW), particularly Lamb waves, has become one of the most popular techniques for damage location, due to their sensitivity to defects, large range of inspection, and good propagation in several material types. However, extracting meaningful physical features from the response signals is challenging due to several factors, such as the multimodal nature of UGW, boundary conditions and the geometric shape of the structure, possible material anisotropies, and their environmental dependency. Neural networks (NN) are becoming a practical and accurate approach to analyzing the acquired data using data-driven methods. In this paper, a Convolutional-Neural-Network (CNN) is proposed to predict the distance-to-damage values from the signals corresponding to a transmitter-receiver path of transducers. The NN input is a 2D image (time-frequency) obtained as the Wavelet transform of the acquired experimental signals. The distances obtained with the NN are the input of a novel damage location algorithm which outputs a bidimensional image of the structure's surface showing the estimated damage locations with a deviation of the actual position lower than 15 mm.

Informed Consent for Mobile Phone Health Surveys in Colombia: A Qualitative Study.

Public health surveys deployed through automated mobile phone calls raise a set of ethical challenges, including succinctly communicating information necessary to obtain respondent informed consent. This study aimed to capture the perspectives of key stakeholders, both experts and community members, on consent processes and preferences for participation in automated mobile phone surveys (MPS) of non-communicable disease risk factors in Colombia. We conducted semi-structured interviews with ethics and digital health experts and focus group discussions with community representatives. There was meaningful disagreement within both groups regarding the necessity of consent, when the purpose of a survey is to contribute to the formulation of public policies. Respondents who favored consent emphasized that consent communications ought to promote understanding and voluntariness, and implicitly suggested that information disclosure conform to a reasonable person standard. Given the automated and unsolicited nature of the phone calls and concerns regarding fraud, trust building was emphasized as important, especially for national MPS deployment. Community sensitization campaigns that provide relevant contextual information (such as the name of the administering institution) were thought to support trust-building. Additional ways to achieve the goals of consent while building trust in automated MPS for disease surveillance should be evaluated in order to inform ethical and effective practice.

Influence and Compensation of Temperature Effects for Damage Detection and Localization in Aerospace Composites.

Structural Health Monitoring (SHM) of Carbon Fiber Reinforced Polymers (CFRP) has become, recently, in a promising methodology for the field of Non-Destructive Inspection (NDI), specially based on Ultrasonic Guided Waves (UGW), particularly Lamb waves using Piezoelectric Transducers (PZT). However, the Environmental and Operational Conditions (EOC) perform an important role on the physical characteristics of the waves, mainly the temperature. Some of these effects are phase shifting, amplitude changes and time of flight (ToF) variations. In this paper, a compensation method for evaluating and compensating the effects of the temperature is carried out, performing a data-driven methodology to calculate the features from a dataset of typical temperature values obtained from a thermoset matrix pristine plate, with a transducer network attached. In addition, the methodology is tested on the same sample after an impact damage is carried out on it, using RAPID (Reconstruction Algorithm for Probabilistic Inspection of Damage) and its geometrical variant (RAPID-G) to calculate the location of the damage.

A DIY Low-Cost Wireless Wind Data Acquisition System Used to Study an Arid Coastal Foredune.

Environmental studies on coastal dune systems are faced with a considerable cost barrier due to the cost of the instrumentation and sensory equipment required for data collection. These systems play an important role in coastal areas as a protection against erosion and as providers of stability to coastal sedimentary deposits. The DIY (Do-It-Yourself) approach to data acquisition can reduce the cost of these environmental studies. In this paper, a low-cost DIY wireless wind data acquisition system is presented which reduces the cost barrier inherent to these types of studies. The system is deployed for the analysis of the foredune of Maspalomas, an arid dune field situated on the south coast of Gran Canaria (Canary Islands, Spain), for the specific purpose of studying the dynamics of a dune type (tongue dunes), which is typical of this environment. The results obtained can be of interest for the study of these coastal environments at both the local level, for the management of this particular dune field, and at the general level for other similar dune fields around the world.

Effect of Metarhizium anisopliae (Ascomycete), Cypermethrin, and D-Limonene, Alone and Combined, on Larval Mortality of Rhipicephalus sanguineus (Acari: Ixodidae).

The effect of the fungus Metarhizium anisopliae Ma14 strain, D-limonene, and cypermethrin, alone and combined, on the mortality of Rhipicephalus sanguineus Latreille larvae was evaluated. Eight separate groups with 25 tick larvae were inoculated with the fungus, cypermethrin, and D-limonene, and four groups were used as untreated controls. The groups were inoculated with serial dilutions of each treatment material: for example, conidial concentrations were 1 × 101, 1 × 102, 1 × 103, 1 × 104, 1 × 105, 1 × 106, 1 × 107, and 1 × 108. A complete randomized experimental design was used. Significant differences were obtained between fungal concentrations, with larval mortalities ranging from 29 to 100%; the D-limonene concentrations showed significant differences, with mortalities that ranged from 47.9 to 82.6%, and cypermethrin mortalities ranged from 69.9 to 89.9% when each was applied alone. In the combined application, the serial dilution of the Ma14 fungus plus cypermethrin at 0.1% concentration caused mortalities ranging from 92.9 to 100%; the mix of serially diluted Ma14 plus D-limonene at 0.1% caused mortalities from 10.3 to 100%; and the mix consisting of serially diluted D-limonene plus cypermethrin at 0.1% caused mortalities from 7.4 to 35.9%. Further laboratory and field research could show that these materials, alone and in combinations, are useful in future tick management and control programs.

Risks Involved in the Use of Enrofloxacin for Salmonella Enteritidis or Salmonella Heidelberg in Commercial Poultry.

The objectives of the present study were to evaluate the risks involved in the use of Enrofloxacin for Salmonella Enteritidis (SE) or Salmonella Heidelberg (SH) in commercial poultry and determine the effects of a probiotic as an antibiotic alternative. Two experiments were conducted to evaluate the risks involved in the use of Enrofloxacin for SE or SH in commercial poultry. Experiment 1 consisted of two trials. In each trial, chickens were assigned to one of three groups; control + SE challenged; Enrofloxacin 25 mg/kg + SE; and Enrofloxacin 50 mg/kg + SE. Chickens received Enrofloxacin in the drinking water from days 1 to 5 of age. On day 6, all groups received fresh water without any treatment. All chickens were orally gavaged with 10(7) cfu/chick of SE at 7 days of age and euthanized on 8 days of age. In Experiment 2, turkey poults were assigned to one of the three groups; control + SH; probiotic + SH; and Enrofloxacin 50 mg/kg + SH. Poults received probiotic or Enrofloxacin in the drinking water from days 1 to 5 of age. On day 6, poults received fresh water without any treatment. Poults were orally gavaged with 10(7) cfu/poult of SH at 7 days of age. Poults were weighed and humanely killed 24 h post-SH challenge to evaluate serum concentration of fluorescein isothiocyanate-dextran to evaluate intestinal permeability, metagenomics, and SH infection. In both trials of Experiment 1, chickens treated with Enrofloxacin were more susceptible to SE organ invasion and intestinal colonization when compared with control non-treated chickens (P < 0.05). In Experiment 2, poults treated with 50 mg/kg of Enrofloxacin showed an increase in body weight, however, this group also showed an increase in SH susceptibility, intestinal permeability, and lower proportion of Firmicutes and Bacteroidetes, but with control group had the highest proportion of Proteobacteria. By contrast, poults that received the probiotic had the highest proportion of Firmicutes and Bacteroidetes, but lowest Proteobacteria. The results of the present study suggest that prophylactic utilization of Enrofloxacin at five times the recommended dose in poultry increases the susceptibility to salmonellae infections, and confirms that probiotics may be an effective tool in salmonellae infections.

La aceptabilidad jurídica de Ia técnica de gestación de vida humana por sustitución de vientre / No disponible

En el presente artículo se expondrán algunos de los argumentos que se esgrimen sobre la aceptabilidad jurídica de la técnica de gestación de vida humana por sustitución de vientre, comenzando por establecer las múltiples denominaciones que de ella se han realizado y que han conllevado a conocerla con los nombres de alquiler de vientre, maternidad subrogada o maternidad sustituta. Posteriormente se determinarán los aspectos legales que conlleva el tránsito de la técnica a su realización a través de contratos, precisando de esta manera los elementos esenciales (capacidad, consentimiento, causa y objeto licito), el contenido y la posible forma de los contratos de gestación de vida humana por sustitución de vientre (AU)

This article will present some of the arguments that have been exposed about the legal acceptability in the technique of gestation of human life by surrogacy, beginning for establish the multiple denominations that have been realized and which make know this technique as: surrogacy, surrogate motherhood or substitute motherhood. Then it will determine the legal aspects involved in the transit of this technique to its accomplishment through contracts, thereby specifying the essential elements (capacity, consent, cause and lawful purpose), the content and the possible manner of gestation human life contracts by surrogacy (AU)

[Not Available]. / La aceptabilidad jurídica de la técnica de gestación de vida humana por sustitución de vientre.

This article will present some of the arguments that have been exposed about the legal acceptability in the technique of gestation of human life by surrogacy, beginning for establish the multiple denominations that have been realized and which make know this technique as: surrogacy, surrogate motherhood or substitute motherhood. Then it will determine the legal aspects involved in the transit of this technique to its accomplishment through contracts, thereby specifying the essential elements (capacity, consent, cause and lawful purpose), the content and the possible manner of gestation human life contracts by surrogacy.

Ultrasonic wave-based structural health monitoring embedded instrument.

Piezoelectric sensors and actuators are the bridge between electronic and mechanical systems in structures. This type of sensor is a key element in the integrity monitoring of aeronautic structures, bridges, pressure vessels, wind turbine blades, and gas pipelines. In this paper, an all-in-one system for Structural Health Monitoring (SHM) based on ultrasonic waves is presented, called Phased Array Monitoring for Enhanced Life Assessment. This integrated instrument is able to generate excitation signals that are sent through piezoelectric actuators, acquire the received signals in the piezoelectric sensors, and carry out signal processing to check the health of structures. To accomplish this task, the instrument uses a piezoelectric phased-array transducer that performs the actuation and sensing of the signals. The flexibility and strength of the instrument allow the user to develop and implement a substantial part of the SHM technique using Lamb waves. The entire system is controlled using configuration software and has been validated through functional, electrical loading, mechanical loading, and thermal loading resistance tests.

Extended-spectrum beta-lactamase-producing bacteria in a tertiary care hospital in Madrid: epidemiology, risk factors and antimicrobial susceptibility patterns.

INTRODUCTION: Extended-spectrum beta-lactamase (ESBL) producing bacteria have been increasingly reported as causal agents of nosocomial infection worldwide. Resistance patterns vary internationally, and even locally, from one institution to the other. We investigated the clinical isolates positive for ESBL-producing bacteria in our institution, a tertiary care hospital in Madrid (Spain), during a 2-year period (2007-2008). METHODS: Clinical and microbiological data were retrospectively reviewed. Two hundred and nineteen patients were included in the study. RESULTS: Advanced age, diabetes, use of catheters, previous hospitalization and previous antibiotic treatment were some of the risk factors found among patients. Escherichia coli was the most frequent isolate, and urinary tract the most common site of isolation. Internal Medicine, Intensive Care Unit (ICU) and General Surgery presented the highest number of isolates. There were no outbreaks during the study period. Antibiotic patterns showed high resistance rates to quinolones in all isolates. There was 100% sensitivity to carbapenems. CONCLUSION: Carbapenems continue to be the treatment of choice for ESBL-producing bacteria. Infection control measures are of great importance to avoid the spread of these nosocomial infections.

Teoría Crítica, riesgo y justicia en salud pública / Critical Theory, Risk and Justice in Public Health / Teoria, crítica, risco e justiça em saúde pública

En este trabajo discuto la pertinencia del programa de Teoría crítica de la sociedad para examinar las demandas de la justicia en salud pública desde una perspectiva no reduccionista, es decir, no sectorial, que a la vez que dé cuenta de la relación íntima que existe entre arreglos sociales y salud permita explorar un poco la pregunta, más fundamental, por las condiciones necesarias para la autorrealización humana...

In this paper I discuss the relevance of the program of social critical theory to examine the demands for justice in public health from a non-reductionist perspective —that is, a not sectorial one— that accounts for the intimate relationship between health and socialarrangements and allows further exploration of the fundamental question about the necessary conditions for human self-realization...

Neste artigo discuto a pertença do programa da Teoria crítica da sociedade para examinar exigências da justiça em saúde pública a partir de uma perspectiva não reducionista, isto é,não setorial, embora consciente da relação íntima existente entre arranjos sociais e saúde permite descobrir um pouco a questão mais fundamental, das condições necessárias à auto-realização humana...