Estudo in vitro do potencial citotóxico da Annona muricata L / In vitro study of the cytotoxic potential of Annona muricata L

O presente trabalho tem como objetivo avaliar a atividade citotóxica do extrato etanólico da casca do caule (AMC) e folha (AMF) da Annona muricata Linn. Para a realização desse estudo, inicialmente foi verificada a atividade do extrato etanólico nas concentrações de 1000, 800, 600, 200 e 100 ?gmL-1 para AMF e concentrações de 200, 150, 100, 50, 10 ?gmL-1 para AMC através do ensaio de Artemia salina Leach que é considerado um bioensaio preliminar no estudo de extratos com forte atividade biológica e permite realizar a avaliação da toxicidade envolvendo apenas um parâmetro: vida ou morte. Posteriormente foi realizado o ensaio de citotoxicidade através do método do MTT (3-(4,5-dimetiltiazol-2yl)-2,5-difenil brometo de tetrazolina em linhagens de SF-295 (glioblastoma - humano), OVCAR-8 (ovário) HCT-116 (colón) e HL-60 (leucemia pormielocítica). Os extratos foram testados na concentração de 50 ?g/mL para o teste de citotoxicidade de concentração única para verificar ausência ou presença de atividade. Para a determinação da concentração inibitória (CI50), todas as amostras foram testadas em concentrações seriadas que variaram de 0,09 a 50 ?g/mL utilizando 2 como fator de diluição. No presente estudo, as duas amostras utilizadas através do ensaio de Artemia salina Leach apresentaram concentração letal (CL50) superiores a 80 ?gmL-1. A folha apresentou CL50 = 324, 07?gmL-1 e a casca do caule apresentou CL50 = 196, 04?gmL-1. Já através do teste do MTT os valores de concentração inibitória (CI50) variaram de 12,81 a 22,65 ?g/mL para AMF e de 0,09 a <5 ?g/mL para AMC, frente as diferentes linhagens tumorais avaliadas. Diante dos resultados obtidos para a casca e folhas de A. muricata, avaliadas neste trabalho através dos bioensaios de toxicidade com Artemia salina Leach e com as células tumorais SF- 295, OVCAR-8, HCT-116 e HL-60, pode-se verificar o potencial tóxico de ambas as amostras, destacando-se mais as cascas que tiveram um potencial citotóxico maior. Devido a este fato, novos experimentos devem ser conduzidos para investigar melhor o potencial antitumoral das cascas do caule de A. muricata .(AU)

This study aims to evaluate the cytotoxic activity of the ethanol extract of the stem bark (AMC) and leaf (MFA) of Annona muricata Linn. To conduct this study was initially verified the activity of the ethanol extract at concentrations of 1000, 800 , 600 , 200 and 100 ?gmL-1 for MFA and concentrations of 200 , 150 , 100 , 50 , 10 ?gmL-1 for AMC through assay of Artemia salina Leach which is considered a preliminary study on bioassay of extracts with strong biological activity. Subsequently, the cytotoxicity assay was performed using the MTT (method 3 - (4,5 -dimethylthiazol- 2YL ) -2,5- diphenyl bromide in tetrazolina lines SF- 295 (glioblastoma - human), OVCAR -8 (ovarian) HCT -116 (colon) and HL -60 (promyelocytic leukemia). The extracts were tested at a concentration of 50 ?g/mL for the single concentration cytotoxicity test to determine the presence or absence of activity. For determination of inhibitory concentration (IC50), all samples were tested in serial concentrations ranging from 0, 09 to 50 ?g/mL using 2 as the dilution factor. The present study, both samples used by testing Artemia salina Leach had higher LC50 80 ?gmL-1. Sheet presented LC50 = 324, 07 ?gmL-1 and stem bark showed LC50 = 196, 04 ?gmL-1. Already through MTT inhibitory concentration values (IC50) ranged from 12, 81 to 22,65 ?g/mL for MFA and from 0,09 to <5?g/ mL for AMC, evaluated against various tumor cell lines. Results obtained for the bark and leaves of A. muricata, evaluated in this work through toxicity bioassays with Artemia salina L and tumor cells SF-295, OVCAR-8, HCT-116 and HL-60, we can verify the toxic potential of both samples, especially more hulls which had a higher cytotoxic potential. Due to this fact, further experiments should be conducted to further investigate the antitumor potential of the stem bark of A. muricata.(AU)

Antibacterial Activity of Lantana camara Linn and Lantana montevidensis Brig Extracts from Cariri-Ceará, Brazil.

The use of medicinal plants with therapeutics properties represents a secular tradition in different cultures, mainly in underdeveloped countries. Lantana camara Linn and Lantana montevidensis Briq (Verbenaceae) found in tropical and subtropical areas around the world are popularly known as "camará" or "chumbinho." In popular medicines, both plants are used as antipyretic and carminative and in the treatment of respiratory system infections. In this study, the antibacterial activity of the ethanolic extracts of L. camara and L. montevidensis leaves and roots against gram-positive and gram-negative strains standard and multi-resistant bacteria isolated from clinical material are presented. In order to determine the minimal inhibitory concentration (MIC), the microdilution method was used. The extracts demonstrated antibacterial activity against all tested bacteria, but the L. montevidensis fresh leaves extract present the best result against P. aeruginosa (MIC 8 µg/mL) and against multi-resistant E. coli (Ec 27) (MIC 16 µg/mL). These results drive new researches with both species in order to isolate the constituents responsible for the activity.