Ten-vertex rhodadithiaborane chemistry:.

Neutral 8-(5-iodo-n-pentyl)-3-(eta(5)-pentamethylcyclopentadienyl)-arachno-3-rhoda-7,8-dithiaundecaborane, [Rh(C(5)H(19)B(8)IS(2))(C(10)H(15))], obtained from the [arachno-7,8-S(2)B(9)H(10)](-) anion by treatment with I(CH(2))(5)I followed by [Rh(C(5)Me(5))Cl(2)](2) and N,N,N',N'-tetramethyl-1,8-diaminonaphthalene, has the 11-vertex cluster geometry of [arachno-7,8-S(2)B(9)H(10)](-), but with an [Rh(C(5)Me(5))] unit in the 3-position instead of a [BH] unit, and with a -(CH(2))(5)I chain attached exo to an S atom.

A case of organic mania associated with open heart surgery.

Organic mania has been reported to have multiple etiologies. A case is described of a patient who developed mania following a coronary artery bypass graft and mitral valve replacement. Cerebral abnormalities were not detected by computed tomographic or magnetic resonance imaging scans, but an area of dysfunction was found using single photon emission computed tomographic (SPECT) imaging. The lesion resolved when the patient became clinically asymptomatic. The area of decreased cerebral perfusion associated with the patient's mania was in an atypical location, raising questions about which brain regions can result in well-defined psychiatric syndromes.

Expression in Escherichia coli of cDNA fragments encoding the gene for the host-protective antigen of infectious bursal disease virus.

The larger segment of the IBDV genome codes for a 32-kDa host-protective antigen. Inserts from a cDNA library in pBR 322, containing overlapping cDNA fragments of varying sizes and covering the entire large segment of the IBDV genome, were subcloned into a mixture of expression vectors pUR 290, 291, and 292. Clones expressing the host-protective antigen, or parts of it, were identified by an immunoblot assay and the fusion proteins were further characterized by Western blot analysis using a monoclonal antibody specific for the 32-kDa polypeptide. Hybridization of inserts from expressing clones to the original cDNA library led to the identification of the region of the IBDV genome that codes for the 32-kDa host-protective antigen. Clone D1 which encodes approximately 50% and clone D6 which encodes the entire 32-kDa protein were selected for further studies. The fusion proteins from clones D1 and D6 were affinity purified and tested for their immunogenicity in chickens. Both fusion proteins induced the synthesis of antibodies in both primed and unprimed chickens that reacted specifically with denatured 32-kDa viral protein, but less well with intact virus. It was concluded that the response to the fusion proteins was to linear rather than conformational epitopes on the 32-kDa viral protein.

The characterization and molecular cloning of the double-stranded RNA genome of an Australian strain of infectious bursal disease virus.

The genome of infectious bursal disease virus (IBDV) strain 002-73 was found to consist of two segments of double-stranded (ds) RNA which were 3400 bp (MW 2.06 X 10(6)) and 2900 bp (MW 1.76 X 10(6)) long, respectively. The ds IBDV RNA could be translated, in vitro, only after extensive denaturation. The small RNA segment was found to code for a single polypeptide of MW 90K, while the large RNA segment coded for three major polypeptides of MW 52K, 32K, and 28K, and two minor polypeptides of MW 41K and 16K. The large RNA segment could encode proteins of MW 125K while the MW of the translated products was 169K suggesting that a precursor-product relationship exists between some of the translation products. A method is described for the synthesis of ds cDNA from large ds RNA molecules. Analyses of recombinant colonies showed that inserts covering the entire IBDV genome had been cloned.