Heavy metal concentrations in sharks, rays and chimaeras from the western Mediterranean Sea.

The potential bioaccumulation of pollutants, such as heavy metals, may pose a threat to the western Mediterranean chondrichthyans and human consumers. Therefore, the first extensive assessment of cadmium (Cd), lead (Pb), and copper (Cu) concentrations in the muscle tissue of 17 species of sharks, rays, and chimaeras in this region was conducted via Microwave Assisted Extraction (MAE) and Graphite Furnace Atomic Absorption Spectrometry (GFAAS). Significant differences between species were observed, particularly related to the rabbit fish (Chimaera monstrosa) and the velvet belly lantern shark (Etmopterus spinax), which exceeded the European Union (EU) Commission Regulation 2023/915 threshold of Cd. Overall, heavy metal concentrations correlated negatively with size and trophic level but positively with depth. Although the consumption of these species may entail minimal risk to adult humans, caution is advised, especially for children. These findings are important due to the widespread consumption of chondrichthyans in many western Mediterranean regions.

Fishing for litter, accidental catch in bottom trawl nets along the Catalan coast, Northwestern Mediterranean.

The seafloor of the Mediterranean Sea accumulates marine litter (ML), an area where bottom trawlers operate and can accidentally catch the litter from the seafloor. This study aims to describe and quantify the ML caught by bottom trawlers along the Catalan coast (NW Mediterranean Sea) and estimate the potential of the bottom trawl fleet to extract ML from the area as a Fishing for Litter (FFL) initiative to tackle the ML issue. Marine litter was collected from commercial trawlers and was classified as metal, plastic, rubber, textile, wood, and other waste and weighed (kg) from 305 hauls performed during three years (2019-2021) from 9 different ports at 3 different depths. ML was present in 97 % of the hauls, with plastic being the most abundant material. The composition varied according to zone, port and depth, with the highest densities found in highly urbanized areas (13.75 ± 3.25 kg km-2), which mainly contained plastics (74.3 %). The port of Barcelona had the highest presence of plastics (23.62 ± 6.49 kg km-2), mainly wet wipes. Regarding depth, the continental shelf had the highest density of ML, with 12.24 ± 2.40 kg km-2. The potential ML removal (t year-1) was calculated using fishing effort (hours). It is estimated that the bottom trawlers may potentially remove 237 ± 36 t year-1 of ML in the Catalan coast. FFL initiatives should be part of a multidisciplinary approach to tackle marine litter, which must include prevention, monitoring, and cleaning actions.

Feeding ecology of a Mediterranean endemic mesopredator living in highly exploited ecosystems.

Knowledge of marine predator trophic ecology is essential for defining their ecological role and trophic position in ecosystems. Based on their trophic habits, sharks and batoids occupy higher and medium trophic levels in the food webs, although differences in the trophic preferences exist between species. They are important organisms in marine ecosystems by maintaining the species below them in the food chain and serving as an indicator for ocean health. In comparison to sharks, batoids usually receive less research attention, with very little diet information available. This is true of the speckled ray (Raja polystigma), one of the three endemic batoids in Mediterranean waters. Here, by combining analyses of stomach contents and stable isotopes, we examined the trophic ecology (dietary composition and trophic position) of this ray in the north-western Mediterranean Sea. We also compared its trophic niche with the trophic position of other sympatric elasmobranchs present in same marine ecosystem. The results revealed that R. polystigma mainly consumes shrimps and to a lesser extent crabs, fin-fish, cephalopods, polychaetes and, surprisingly, small demersal sharks. We also found that R. polystigma shows similar trophic position to other crustacean-consumer elasmobranchs such rays and small demersal sharks. The results of this study provide new insights into the ecological role of this endemic ray species in the Mediterranean Sea.

Synthesis and biological evaluation of potential acetylcholinesterase inhibitors based on a benzoxazine core.

With the purpose of expanding the structural variety of chemical compounds available as pharmacological tools for the treatment of Alzheimer's disease, we synthesized and evaluated a novel series of indole-benzoxazinones (Family I) and benzoxazine-arylpiperazine derivatives (Family II) for potential human acetylcholinesterase (hAChE) inhibitory properties. The most active compounds 7a and 7d demonstrated effective inhibitory profiles with Ki values of 20.3 ± 0.9 µM and 20.2 ± 0.9 µM, respectively. Kinetic inhibition assays showed non-competitive inhibition of AChE by the tested compounds. According to our docking studies, the most active compounds from both series (Families I and II) showed a binding mode similar to donepezil and interact with the same residues.

Plastic debris straps on threatened blue shark Prionace glauca.

Juveniles of blue shark Prionace glauca caught in pelagic longlines targeting tuna and swordfish in the Atlantic Ocean and the Mediterranean Sea were found entangled with plastic straps around their gill region. The plastic debris were identified as strapping bands and caused several degrees of injuries on the dorsal musculature and pectoral fins. They were also obstructing the gill slits probably causing breathing issues. These records were uploaded in the web site seawatchers.org, and highlight the potential of citizen science in revealing the occurrence of such problems which could help to measure the effects of plastic debris on marine life.

Synthesis and Docking of Novel 3-Indolylpropyl Derivatives as New Polypharmacological Agents Displaying Affinity for 5-HT1A R/SERT.

A series of novel 3-indolylpropyl derivatives was synthesized and evaluated for their binding affinities at the serotonin-1A receptor subtype (5-HT1A R) and the 5-HT transporter (SERT). Compounds 11b and 14b exhibited the highest affinities at the 5-HT1A R (Ki = 43 and 56 nM), whereas compounds 11c and 14a were the most potent analogs at the SERT (Ki = 34 and 17 nM). On the other hand, compounds 14b and 11d showed potent activity at both targets, displaying a profile that makes them promising leads for the search for novel potent ligands with a dual mechanism of action. Molecular docking studies in all the compounds unveiled relevant drug-target interactions, which allowed rationalizing the observed affinities.

The phylogenetic position of the giant devil ray Mobula mobular (Bonnaterre, 1788) (Myliobatiformes, Myliobatidae) inferred from the mitochondrial genome.

The giant devil ray, Mobula mobular, is a member of one of the most distinct groups of cartilaginous fishes, the Mobulidae (manta and devil rays), and is the only mobulid assessed as Endangered due its restricted distribution, high bycatch mortality and suspected population decline. The complete mitochondrial genome is 18 913 base pairs in length and comprises 2 rRNAs, 13 protein-coding genes, 22 tRNAs and 2 non-coding regions. Comparison with the partial mitogenome of M. japanica suggests a sister-cryptic species complex and two different taxonomic units. However, the limited divergence within the species (>99.9% genetic identity) may be the result of a geographically and numerically restricted population of M. mobular within the Mediterranean Sea.

Observations on the occurrence of Spraguea lophii in Mediterranean lophiids.

In the Mediterranean Sea, anglerfish Lophius budegassa and Lophius piscatorius support an important fishery, and landings have increased in recent years. These species are infected by the microsporean Spraguea lophii, a parasite that infects their nervous system giving rise to multiple cysts. Due to the high value of these fish and conspicuous nature of the infection, we determined the apparent prevalence of this parasite in both Lophius spp. Specimens were obtained from commercial catches along the NW Mediterranean Sea during 2013. The parasite was observed in the nerves from the peripheral nervous system of the individuals and was detected at the prevalence of 69.2% (202/292) and 100% (58/58) in L. budegassa and L. piscatorius, respectively. Non-significant differences were noted between sexes of L. budegassa (χ(2) = 0.683; p = 0.409), although a positive correlation was noted between host size and prevalence (χ(2) = 6.134; p = 0.013). During the sampling, two specimens of L. budegassa with atypical morphological characteristics, pigment anomalies and blindness and infected with S. lophii xenomas were described.

Synthesis, docking and pharmacological evaluation of novel homo- and hetero-bis 3-piperazinylpropylindole derivatives at SERT and 5-HT1A receptor.

A series of 3-(3-(4-(3-(1H-indol-3-yl)propyl)piperazin-1-yl)propyl)-1H-indole derivatives (3a-d and 5a-f) as homo- and hetero-bis-ligands, were synthesized and evaluated for in vitro affinity at the serotonin transporter (SERT) and the 5-HT1A receptor. Compounds 5b and 5f showed nanomolar affinities for both targets. The experimental data were rationalized according to results obtained from docking experiments. These findings are in agreement with our proposal that bis-indole derivatives can bind both targets, and might serve as leads in the quest of ligands endowed with a dual mechanism of action.

Synthesis, 5-hydroxytryptamine1A receptor affinity and docking studies of 3-[3-(4-aryl-1-piperazinyl)-propyl]-1H-indole derivatives.

A series of 3-[3-(4-aryl-1-piperazinyl)-propyl]-1H-indole derivatives (12a-h) was synthesized and evaluated for binding affinity at the human 5-hydroxytryptamine(1A) receptor (5-HT(1A)R) compounds (12b) and (12h) showed the highest 5-HT(1A) receptor affinity (IC(50)=15 nM). Molecular docking studies with all the compounds in a homology model of 5-HT(1A) showed that the main interaction anchoring the ligand in the receptor was a charge-reinforced bond between the protonated nitrogen atom (N-4) of the piperazine ring and Aspartate(3.32).

Synthesis, docking studies and biological evaluation of benzo[b]thiophen-2-yl-3-(4-arylpiperazin-1-yl)-propan-1-one derivatives on 5-HT1A serotonin receptors.

A series of novel benzo[b]thiophen-2-yl-3-(4-arylpiperazin-1-yl)-propan-1-one derivatives 6a-f, 7a-f and their corresponding alcohols 8a-f were synthesized and evaluated for their affinity towards 5-HT(1A) receptors. The influence of arylpiperazine moiety and benzo[b]thiophene ring substitutions on binding affinity was studied. The most promising analogue, 1-(benzo[b]thiophen-2-yl)-3-(4-(pyridin-2-yl)piperazin-1-yl)propan-1-one (7e) displayed micromolar affinity (K(i) = 2.30 µM) toward 5-HT(1A) sites. Docking studies shed light on the relevant electrostatic interactions which could explain the observed affinity for this compound.

The International Study of Wheezing in Infants: questionnaire validation.

BACKGROUND: There are no internationally validated questionnaires to investigate the prevalence of infant wheezing. This study was undertaken to validate a questionnaire for the International Study on the Prevalence of Wheezing in Infants (Estudio Internacional de Sibilancias en Lactantes, EISL). MATERIAL AND METHODS: Construct and criterion validity were tested for the question 'Has your baby had wheezing or whistling in the chest during his/her first 12 months of life?'. Construct validity (i.e. the ability of parents and doctors to refer to the same symptoms with the same words) was tested in a sample of 50 wheezing and 50 non-wheezy infants 12-15 months of age in each of 10 centres from 6 different Spanish- or Portuguese-speaking countries. Criterion validity (i.e. the ability of parents to correctly detect the symptom in the general population) was evaluated in 2 samples (Santiago, Chile and Cartagena, Spain) of 50 wheezing and 50 non-wheezing infants (according to parents) of the same age, randomly selected from the general population, who were later blindly diagnosed by a paediatric pulmonologist. RESULTS: Construct validity was very high (kappa test: 0.98-1) in all centres. According to Youden's index, criterion validity was good both in Cartagena (75.5%) and in Santiago (67.0%). Adding questions about asthma medication did not improve diagnosis accuracy. CONCLUSIONS: The EISL questionnaire significantly distinguished wheezy infants from healthy ones. This questionnaire has a strong validity and can be employed in large international multicentre studies on wheezing during infancy.