Solid waste management and Aedes aegypti infestation interconnections: A regression tree application.

Public health is at the core of all environmental and anthropic impacts. Urban and territorial planners should include public health concerns in their plans. Basic sanitation infrastructure is essential to maintaining public health and social and economic development. This infrastructure deficiency causes diseases, death and economic losses in developing countries. Framing interconnections among health, sanitation, urbanization and circular economy will assist sustainable development goal achievements. This study aims to identify the relationships between solid waste management indicators in Brazil and the Aedes aegypti mosquito infestation index. Regression trees were employed for modelling due to the complexity and characteristics of the data. The analyses were performed separately from data collected from 3501 municipalities and 42 indicators from the country's five regions. Results show that expenses and personnel indicators were the most critical indicators (in the mid-western, southeastern and southern regions), operational (northeastern (NE) region) and management (northern region). The mean absolute errors ranged from 0.803 (southern region) to 2.507 (NE region). Regional analyses indicate that the municipalities with better SWM results display lower infestation rates in buildings and residences. This research is innovative as it analyses infestation rates rather than dengue prevalence, using a machine learning method, in a multidisciplinary research field that needs further study.

Waste management, COVID-19 and occupational safety and health: Challenges, insights and evidence.

Different fields of knowledge have faced challenges due to the COVID-19 pandemic. Essential public services serving the population's needs were maintained. Previous studies have addressed the possibility of health hazards from handling solid waste (SW) among trash collection service workers, without introducing any data proving or dismissing those risks. Occupational health is stated in at least 2 of the 17 Sustainable Development Goals. Pearson Coefficient (r) was employed to analyze the correlation between the number of cases of COVID-19 in SW collection workers in the city of São Paulo, considering the district city halls where the COVID-19 patients live and the SW collection workers' route, from March 2020 to March 2021. Employees who collected wastes with COVID-19: 2% collected healthcare SD, 4% worked at the waste transshipment stations, 10% household variable route collection, and 83% in defined routes collecting household wastes. Positive and moderate correlation was confirmed (r = 0.59) between the total number of COVID-19 cases and the trash collection routes, and the number of contaminated COVID-19 workers from their trash collection routes. Considering concessionary companies separately, the correlation found in the Southeastern grouping is 0.77 (positive and high correlation), and the correlation found in the Northwestern grouping is 0.18 (positive but insignificant correlation). The Northwestern grouping's concessionary had implemented more effective occupational health and safety management policies and disclosed them among their stakeholders. Results suggest concern related to occupational health and safety for SW collection workers and substantiating the vulnerability of this essential activity during the pandemic.

Influência do agente cimentante e desajuste interno na adaptação marginal de coroas em resina nanocerâmica / Influence of the cement agent and internal mismatch in the marginal adaptation of nanoceramic resins crowns

A adaptação marginal é um dos requisitos fundamentais para a longevidade e o sucesso das restaurações. O objetivo desse estudo foi avaliar a influência do desajuste interno associado à diferentes agentes cimentantes na desadaptação marginal de coroas em resina nanocerâmica (Lava Ultimate 3M ESPE) confeccionadas pelo sistema CAD-CAM. A partir do escaneamento de um preparo de coroa total em um pré-molar, foram obtidos 36 modelos em metal. Esses foram divididos em seis grupos de acordo com o desajuste interno (70 µm ou 100 µm) e o cimento (Fosfato de zinco- SS White, Cimento de ionômero de vidro- Ketac Cem 3M/ESPE e Cimento resinoso- Relyx Ultimate 3M/ESPE). Após escaneamento de todos os modelos as coroas foram confeccionadas a partir da fresagem de blocos de resina nanocerâmica (Lava Ultimate 3M/ESPE). Com auxílio de microscópio óptico comparador (40X) foram medidas as desadaptações marginais prévia e posteriormente à cimentação, sendo considerados 8 pontos ao longo do término em todas as faces do corpo de prova. Os valores obtidos após serem submetidos aos testes two-way anova e teste t, demonstraram que as coroas confeccionadas com desajuste interno de 70 µm apresentam desadaptação marginal inferior estatisticamente significante às coroas com desajuste interno de 100 µm. Para o desajuste interno de 70 µm o grupo do cimento fosfato de zinco apresentou o menor gap marginal, sendo que este valor não foi estatisticamente diferente em relação ao grupo do Ketac Cem. Ainda com esse desajuste, o grupo do Ketac Cem não apresentou diferença estatística significante em relação ao grupo do Relyx Ultimate, no entanto esse último promoveu "gap" marginal estatisticamente maior em relação ao grupo do cimento fosfato de zinco. Para o desajuste interno de 100 µm não houve diferença estatisticamente significante no "gap" marginal entre os três cimentos avaliados. O presente estudo demonstrou através da metodologia utilizada, que a menor desadaptação marginal observada nas restaurações de Lava Ultimate, foi proveniente do desajuste interno de 70 µm. Neste grupo os cimentos de fosfato de zinco e de ionômero de vidro demonstraram melhor comportamento comparado ao cimento resinoso, embora o cimento de ionômero de vidro numericamente, mas não estatisticamente tenha apresentado resultado inferior ao do fosfato de zinco

The marginal adaptation is one of the basic requirements for the longevity and success of restorations. The aim of this study was to evaluate the influence of the internal imbalances associated with different luting agents on marginal leakage of crowns Nanoceramic resin ( Lava Ultimate 3M ESPE) made by CAD-CAM system. From the scanning of a full crown preparation in a pre molar were obtained 36 metal models. These were divided into six groups according to the internal imbalances ( 70 or 100 micrometres) and cement (SS White Zinc phosphate, glass ionomer cemet Ketac Cem 3M/ Espe and resinous cement Relyx Ultimate 3M / Espe). After scanning all models crowns were made from milling Nanoceramic resin blocks (Lava Ultimate 3M / Espe). With the aid of optical comparator microscope (40X) were measured prior marginal mismatches and after the cementation, being considered 8 points along the end in all specimen faces. The values were submitted to ANOVA two-way test and t test. Results showded that crowns made with internal imbalances of 70 uM show statistically significant lower marginal leakage to the crows with internal imbalances of 100 micrometres. For the internal imbalances 70 uM zinc phosphate cement group showed the lowest "gap" marginal, and this figure does not show a statistically significant difference compared to Ketac Cem gropu. Even with this misfit, Ketac Cem group showed no statistically significant difference in relation to Relyx Ultimate group, though the latter showed a statistically significant difference compared to zinc phosphate cement group. For the internal imbalances of 100 micrometres there was no statistically significant difference in the "gap" marginal among the three cements. This study demostrated through the methodology used, the lower marginal leakage oberved in Lava Ultimate restorations, was from the inner misfit 70 micrometers...

Documentação fotográfica intra-operatória / Intraoperative photographic documentation

O prontuário médico é um dever ético, sendo a documentação fotográfica uma extensão deste, e muitas vezes de grande importância, principalmente na Cirurgia Plástica. A documentação fotográfica pré-operatória e pós-operatória em Cirurgia Plástica é rotineiramente utilizada, enquanto a fotografia intra-operatória ainda não, apesar de facilitar e auxiliaras divulgações científicas e ser uma forte aliada nos registros médicos. O presente artigo apresenta uma forma simples, de baixo custo, capaz de permitir a documentação fotográfica intra-operatória com ótima qualidade, preservando-se o ângulo de visão do próprio cirurgião, com menores riscos de contaminação, demandando menor tempo de interrupção e independente de auxiliares externos. Tal ferramenta consiste na associação de uma capa à prova de água à câmera fotográfica digital.

The medical records are an ethic obligation and the photographic documentation is part of it; many times with huge importance, mainly in Plastic Surgery. The preoperative and postoperative photographic documentation are commonly used in Plastic Surgery, while the intraoperative photograph is not yet, although it helps and facilitates scientific reports and it is a strong ally in medical records. The present article shows a simple way of taking intraoperative photography documentation, with high quality, low cost, preserving the surgeons angle of vision, with low risk of contamination, demanding less time of interruption and independent of external. This tool consists simply the association of a waterproof case with the digital camera.

Rapid methodology for antigenic profiling of FMDV field strains and for the control of identity, purity and viral integrity in commercial virus vaccines using monoclonal antibodies.

Monoclonal antibodies (MAbs) developed against different foot-and-mouth disease virus (FMDV) vaccine strains were extensively used to study any possible antigenic variations during vaccine production in Argentine facilities. Additionally, a typing ELISA using strain specific MAbs was developed to detect potential cross contaminations among FMDV strains in master and working seeds with high specificity and sensitivity and to confirm strains identity in formulated vaccines. This assay was carried out for the South American strains currently in use in production facilities in Argentina (A24/Cruzeiro, A/Argentina/01, O1/Campos and C3/Indaial) and for the strain O/Taiwan, produced only for export to Asia. These non-cross reactive MAbs were also used to analyze the integrity of viral particles belonging to each one of the individual strains, following isolation of 140S virions by means of sucrose density gradients from the aqueous phase of commercial polyvalent vaccines. Antigenic profiles were defined for FMDV reference strains using panels of MAbs, and a coefficient of correlation of reactivity with these panels was calculated to establish consistent identity upon serial passages of master and production seeds. A comparison of vaccine and field strain antigenic profiles performed using coefficients of correlation allowed the rapid identification of two main groups of serotype A viruses collected during the last FMD epidemic in Argentina, whose reactivity matched closely to A/Argentina/2000 and A/Argentina/2001 strains.

Prenatal stress and early adoption effects on benzodiazepine receptors and anxiogenic behavior in the adult rat brain.

Chronic maternal stress during pregnancy has been associated with behavioral alterations that persist into adulthood. Moreover, adoption procedures performed immediately after birth can reverse these alterations. In this study, we examined the effects of prenatal restraint stress and adoption at birth (cross-fostering) on the behavioral response to an anxiety-provoking situation and on the adult male offspring expression of benzodiazepine (BDZ) receptors in selected brain areas. Adult offspring of rats stressed during the last week of pregnancy exhibited higher levels of anxiety than control rats. The anxiogenic behavior found at the elevated plus maze (EPM) has been related to the reduced levels of BDZ receptor levels in specific brain areas. Adult offspring of rats stressed during pregnancy exhibited a decrease in the number of BDZ receptors binding sites in the central amygdaloid nucleus (Ce), CA1, CA3, and the dentate gyrus regions of the hippocampus when compared to controls. Regarding the adoption procedure, control pups raised by a foster gestationally stressed mother showed similar levels of anxiety as stressed groups. Stressed offspring raised by a foster control mother showed reduced anxiety levels compared to that of the control groups. Adoption per se showed no difference in time spent, neither in the open arms of the plus maze nor in BDZ receptor levels, when compared to the corresponding control and stressed groups. Stressed offspring raised by a foster control mother reverted BDZ receptor levels to control values. However, control pups raised by a gestationally stressed foster mother showed similar values compared to the control offspring in hippocampus, in spite of showing an anxiogenic behavior in the EPM. We found a significant increase of Ce BDZ receptor levels in control offspring raised by a foster stressed mother that could be explained as a compensatory effect to a GABA receptor desensitization. In summary, the behavioral outcome of the adult offspring is vulnerable both to the stress experience during the late prenatal period as well as to possible variations in care during lactation by mothers subjected to chronic stress during gestation. There seems to be a direct correlation between anxiety state and BDZ receptor levels in the adult offspring raised by their biological mothers. However, the mechanism of BDZ regulation leading to an anxious behavior might be different if the insult is received only postnatally as opposed to both pre and postnatally.

Morphine withdrawal syndrome and its prevention with baclofen: Autoradiographic study of mu-opioid receptors in prepubertal male and female mice.

Although the expression of the morphine (MOR) withdrawal syndrome is more marked in male mice than in females, we have demonstrated that the GABAB agonist baclofen (BAC) is able to attenuate MOR withdrawal signs in either sex. In order to extend these previous observations, the aim of the present study was to evaluate the mu-opioid receptor labeling in various brain areas in mice of either sex, during MOR withdrawal and its prevention with BAC. Prepubertal Swiss-Webster mice were rendered dependent by intraperitonial (i.p.) injection of MOR (2 mg/kg) twice daily for 9 days. On the 10th day, dependent animals received naloxone (NAL; 6 mg/kg, i.p.) 60 min after MOR, and another pool of dependent mice received BAC (2 mg/kg, i.p.) previous to NAL. Thirty minutes after NAL, mice were sacrificed and autoradiography with [3H]-[D-Ala2, N-Me-Phe4, -glycol5] enkephalin (DAMGO) was carried out on mice brains at five different anatomical levels. Autoradiographic mapping showed a significant increase of mu-opioid receptor labeling during MOR withdrawal in nucleus accumbens core (NAcC), caudate putamen (CPu), mediodorsal thalamic nucleus (MDTh), basolateral and basomedial amygdala, and ventral tegmental area vs. respective control groups in male mice. In contrast, opiate receptor labeling was not significantly modified in any of the brain areas studied in withdrawn females. BAC reestablished mu-opioid receptor binding sites during MOR withdrawal only in NAcC of males, and a similar tendency was observed in CPu and MDTh, even when it was not statistically significant. The sexual dimorphism observed in the present study confirms previous reports indicating a greater sensitivity of males in response to MOR pharmacological properties. The present results suggest that the effect of BAC in preventing the expression of MOR withdrawal signs could be related with the ability of BAC to reestablish the mu-opioid receptor labeling in certain brain areas.

Astrocyte-neuron vulnerability to prenatal stress in the adult rat brain.

Chronic activation of the stress response during pregnancy has been shown to be injurious to the development of the offspring. We have previously demonstrated that restraint prenatal stress inflicted during the last week of pregnancy in rats increased dopamine and glutamate receptors in forebrain areas of the adult offsprings. In this study, the same prenatal insult was employed to assess morphological changes in astrocytes and in the dendritic arborization in frontal cortex, striatum, and hippocampus of the adult rat brain. On postnatal day 90, brains were processed for immunocytochemistry using primary antibodies to glial fibrillary acidic protein (GFAP; the main cytoskeletal astroglial protein), S100B protein (an astroglial-derived neurotrophic factor), MAP-2 (a microtubule-associated protein present almost exclusively in dendrites), and synaptophysin (Syn; one major integral protein of the synaptic vesicles membrane). The results show a significant increase in the cell area of GFAP-immunoreactive (-IR) astrocytes, with high levels of S100B protein and a significant decrease in the relative area of MAP-2-IR neuronal processes in prenatally stressed adult rats. The expression of synaptophysin decreased in all areas studied. These results demonstrate that prenatal stress induces a long-lasting astroglial reaction and a reduced dendritic arborization, with synaptic loss in the brain of adult offspring. In addition to the neurochemical alterations previously reported, these morphological changes might be underlying the behavioral and learning impairment previously observed in prenatally stressed rats.

Early adoption modifies the effects of prenatal stress on dopamine and glutamate receptors in adult rat brain.

Stressful stimuli during pregnancy induce complex effects that influence the development of offspring. These effects can be prevented by environmental manipulations during the early postnatal period. Repeated restraint during the last week of pregnancy was used as a model of prenatal stress, and adoption at birth was used to change the postnatal environment. No differences were found in various physical landmarks, except for testis descent, for which all prenatally stressed pups showed a 1-day delay in comparison with control rats, regardless of the postnatal adoption procedure. Levels of dopamine (DA) D(2) and glutamate (Glu) N-methyl-D-aspartate (NMDA) receptors were differentially regulated in different forebrain regions of cross-fostered adult offspring. Increased concentrations of cortical D(2) receptors detected in stressed pups, raised by a gestationally stressed biological mother, were not detected when the pups were raised by a control mother. Control pups raised by a foster mother whether gestationally stressed or not had higher levels of NMDA receptors in cortical areas. These findings suggest that the normal expression of DA and Glu receptors is influenced by in utero experience and by lactation. The complex pattern of receptor changes reflects the high vulnerability of DA and Glu systems to variations both in prenatal and in postnatal environment, particularly for cortical D(2) receptors and NMDA receptors in cerebral cortex and nucleus accumbens. In contrast, testis descent appears to be more susceptible to prenatal than to postnatal environmental events.

Corticosterone down-regulates dopamine D4 receptor in a mouse cerebral cortex neuronal cell line.

We have previously reported that restraint stress applied to the gestant mother results in long-lasting effects in the offspring that show an increase in the number of dopamine D2-type receptors in limbic areas on the adult rat brain cortex. Evidence that stress during pregnancy results in activation of the hypothalamic-pituitary-adrenal (HPA) axis has been extensively demonstrated. Therefore, high levels of corticosterone secreted in response to stress by the gestant mother might be one of the predisposing factors for the changes observed in dopamine receptors in the adult rat brain. In this study we addressed the question whether corticosterone would directly up-regulate D2-type receptors in vitro. We have investigated the effect of different concentrations of corticosterone on D4 dopamine receptor in immortalized cell lines from cerebral cortex of normal mouse fetuses, detected by immunocytochemistry employing polyclonal antibodies generated against synthetic peptides homologous to an extracellular domain of D4 receptor. The results show that corticosterone in vitro decreases the number of dopamine D4 receptors, suggesting that the increase of D2-type receptors in adult rats following prenatal stress is not related to a direct action of corticosterone on receptor expression.

Long-term effects of prenatal stress on dopamine and glutamate receptors in adult rat brain.

Prenatal stress greatly influences the ability of an individual to manage stressful events in adulthood. Such vulnerability may result from abnormalities in the development and integration of forebrain dopaminergic and glutamatergic projections during the prenatal period. In this study, we assessed the effects of prenatal stress on the expression of selective dopamine and glutamate receptor subtypes in the adult offsprings of rats subjected to repeated restraint stress during the last week of pregnancy. Dopamine D2-like receptors increased in dorsal frontal cortex (DFC), medial prefrontal cortex (MPC), hippocampal CA1 region and core region of nucleus accumbens (NAc) of prenatally stressed rats compared to control subjects. Glutamate NMDA receptors increased in MPC, DFC, hippocampal CA1, medial caudate-putamen, as well as in shell and core regions of NAc. Group III metabotropic glutamate receptors increased in MPC and DFC of prenatally stressed rats, but remained unchanged in all other regions examined. These results indicate that stress suffered during the gestational period has long lasting effects that extend into the adulthood of prenatally stressed offsprings. Changes in dopamine and glutamate receptor subtype levels in different forebrain regions of adult rats suggest that the development and formation of the corticostriatal and corticolimbic pathways may be permanently altered as a result of stress suffered prenatally. Maldevelopment of these pathways may provide a neurobiological substrate for the development of schizophrenia and other idiopathic psychotic disorders.

Controle corporativo no Brasil e o papel dos fundos de pensäo: um enfoque institucional / Corporate governance in Brazil and the pension funds paper: a institutional view

Abordando a questäo da previdência social, analisa o papel dos fundos de pensäo no Brasil e no mundo. Analisa a teoria do controle corporativo como suporte para um melhor entendimento do papel que os fundos de pensäo poderäo desempenhar na economia brasileira após a estabilizaçäo.