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1.
FEBS Lett ; 589(20 Pt A): 2975-86, 2015 Oct 07.
Article in English | MEDLINE | ID: mdl-26096785

ABSTRACT

Advanced techniques including the chromosome conformation capture (3C) methodology and its derivatives are complementing microscopy approaches to study genome organization, and are revealing new details of three-dimensional (3D) genome architecture at increasing resolution. The fission yeast Schizosaccharomyces pombe (S. pombe) comprises a small genome featuring organizational elements of more complex eukaryotic systems, including conserved heterochromatin assembly machinery. Here we review key insights into genome organization revealed in this model system through a variety of techniques. We discuss the predominant role of Rabl-like configuration for interphase chromosome organization and the dynamic changes that occur during mitosis and meiosis. High resolution Hi-C studies have also revealed the presence of locally crumpled chromatin regions called "globules" along chromosome arms, and implicated a critical role for pericentromeric heterochromatin in imposing fundamental constraints on the genome to maintain chromosome territoriality and stability. These findings have shed new light on the connections between genome organization and function. It is likely that insights gained from the S. pombe system will also broadly apply to higher eukaryotes.


Subject(s)
Chromosomes, Fungal/genetics , Genome, Fungal , Schizosaccharomyces/genetics , Animals , Chromosomes, Fungal/ultrastructure , Gene Order , Heterochromatin/physiology , Heterochromatin/ultrastructure , Humans , Meiosis , Schizosaccharomyces/cytology
2.
Nature ; 516(7531): 432-435, 2014 Dec 18.
Article in English | MEDLINE | ID: mdl-25307058

ABSTRACT

Eukaryotic genomes are folded into three-dimensional structures, such as self-associating topological domains, the borders of which are enriched in cohesin and CCCTC-binding factor (CTCF) required for long-range interactions. How local chromatin interactions govern higher-order folding of chromatin fibres and the function of cohesin in this process remain poorly understood. Here we perform genome-wide chromatin conformation capture (Hi-C) analysis to explore the high-resolution organization of the Schizosaccharomyces pombe genome, which despite its small size exhibits fundamental features found in other eukaryotes. Our analyses of wild-type and mutant strains reveal key elements of chromosome architecture and genome organization. On chromosome arms, small regions of chromatin locally interact to form 'globules'. This feature requires a function of cohesin distinct from its role in sister chromatid cohesion. Cohesin is enriched at globule boundaries and its loss causes disruption of local globule structures and global chromosome territories. By contrast, heterochromatin, which loads cohesin at specific sites including pericentromeric and subtelomeric domains, is dispensable for globule formation but nevertheless affects genome organization. We show that heterochromatin mediates chromatin fibre compaction at centromeres and promotes prominent inter-arm interactions within centromere-proximal regions, providing structural constraints crucial for proper genome organization. Loss of heterochromatin relaxes constraints on chromosomes, causing an increase in intra- and inter-chromosomal interactions. Together, our analyses uncover fundamental genome folding principles that drive higher-order chromosome organization crucial for coordinating nuclear functions.


Subject(s)
Cell Cycle Proteins/metabolism , Chromosomal Proteins, Non-Histone/metabolism , Genome, Fungal , Heterochromatin/metabolism , Schizosaccharomyces pombe Proteins/metabolism , Schizosaccharomyces/physiology , Molecular Conformation , Schizosaccharomyces/genetics , Cohesins
3.
Am J Med Genet A ; 161A(7): 1599-611, 2013 Jul.
Article in English | MEDLINE | ID: mdl-23666920

ABSTRACT

Hutchinson-Gilford Progeria Syndrome (HGPS) is a premature aging disorder caused by mutations in LMNA, which encodes the nuclear scaffold proteins lamin A and C. In HGPS and related progerias, processing of prelamin A is blocked at a critical step mediated by the zinc metalloprotease ZMPSTE24. LMNA-linked progerias can be grouped into two classes: (1) the processing-deficient, early onset "typical" progerias (e.g., HGPS), and (2) the processing-proficient "atypical" progeria syndromes (APS) that are later in onset. Here we describe a previously unrecognized progeria syndrome with prominent cutaneous and cardiovascular manifestations belonging to the second class. We suggest the name LMNA-associated cardiocutaneous progeria syndrome (LCPS) for this disorder. Affected patients are normal at birth but undergo progressive cutaneous changes in childhood and die in middle age of cardiovascular complications, including accelerated atherosclerosis, calcific valve disease, and cardiomyopathy. In addition, the proband demonstrated cancer susceptibility, a phenotype rarely described for LMNA-based progeria disorders. The LMNA mutation that caused LCPS in this family is a heterozygous c.899A>G (p.D300G) mutation predicted to alter the coiled-coil domain of lamin A/C. In skin fibroblasts isolated from the proband, the processing and levels of lamin A and C are normal. However, nuclear morphology is aberrant and rescued by treatment with farnesyltransferase inhibitors, as is also the case for HGPS and other laminopathies. Our findings advance knowledge of human LMNA progeria syndromes, and raise the possibility that typical and atypical progerias may converge upon a common mechanism to cause premature aging disease.


Subject(s)
Lamin Type A/genetics , Mutation , Progeria/genetics , Adult , Age of Onset , Animals , Atherosclerosis/genetics , Cardiovascular Diseases/genetics , Enzyme Inhibitors/pharmacology , Farnesyltranstransferase/antagonists & inhibitors , Farnesyltranstransferase/metabolism , Female , Fibroblasts/drug effects , Fibroblasts/pathology , Genes, Dominant , Genetic Predisposition to Disease , Heterozygote , Humans , Lamin Type A/metabolism , Male , Mice , NIH 3T3 Cells , Neoplasms/genetics , Nuclear Envelope/genetics , Nuclear Envelope/ultrastructure , Progeria/complications , Progeria/epidemiology , Progeria/pathology , Protein Modification, Translational , Syndrome
4.
Microbiol Mol Biol Rev ; 76(3): 626-51, 2012 Sep.
Article in English | MEDLINE | ID: mdl-22933563

ABSTRACT

The mating pheromone a-factor secreted by Saccharomyces cerevisiae is a farnesylated and carboxylmethylated peptide and is unusually hydrophobic compared to other extracellular signaling molecules. Mature a-factor is derived from a precursor with a C-terminal CAAX motif that directs a series of posttranslational reactions, including prenylation, endoproteolysis, and carboxylmethylation. Historically, a-factor has served as a valuable model for the discovery and functional analysis of CAAX-processing enzymes. In this review, we discuss the three modules comprising the a-factor biogenesis pathway: (i) the C-terminal CAAX-processing steps carried out by Ram1/Ram2, Ste24 or Rce1, and Ste14; (ii) two sequential N-terminal cleavage steps, mediated by Ste24 and Axl1; and (iii) export by a nonclassical mechanism, mediated by the ATP binding cassette (ABC) transporter Ste6. The small size and hydrophobicity of a-factor present both challenges and advantages for biochemical analysis, as discussed here. The enzymes involved in a-factor biogenesis are conserved from yeasts to mammals. Notably, studies of the zinc metalloprotease Ste24 in S. cerevisiae led to the discovery of its mammalian homolog ZMPSTE24, which cleaves the prenylated C-terminal tail of the nuclear scaffold protein lamin A. Mutations that alter ZMPSTE24 processing of lamin A in humans cause the premature-aging disease progeria and related progeroid disorders. Intriguingly, recent evidence suggests that the entire a-factor pathway, including all three biogenesis modules, may be used to produce a prenylated, secreted signaling molecule involved in germ cell migration in Drosophila. Thus, additional prenylated signaling molecules resembling a-factor, with as-yet-unknown roles in metazoan biology, may await discovery.


Subject(s)
Peptides/genetics , Peptides/metabolism , Progeria/etiology , Saccharomyces cerevisiae/physiology , Animals , Drosophila/metabolism , Drosophila/physiology , Humans , Mating Factor , Peptides/chemistry , Progeria/genetics , Progeria/metabolism , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism , Signal Transduction
5.
Hum Mol Genet ; 21(18): 4084-93, 2012 Sep 15.
Article in English | MEDLINE | ID: mdl-22718200

ABSTRACT

The zinc metalloprotease ZMPSTE24 plays a critical role in nuclear lamin biology by cleaving the prenylated and carboxylmethylated 15-amino acid tail from the C-terminus of prelamin A to yield mature lamin A. A defect in this proteolytic event, caused by a mutation in the lamin A gene (LMNA) that eliminates the ZMPSTE24 cleavage site, underlies the premature aging disease Hutchinson-Gilford Progeria Syndrome (HGPS). Likewise, mutations in the ZMPSTE24 gene that result in decreased enzyme function cause a spectrum of diseases that share certain features of premature aging. Twenty human ZMPSTE24 alleles have been identified that are associated with three disease categories of increasing severity: mandibuloacral dysplasia type B (MAD-B), severe progeria (atypical 'HGPS') and restrictive dermopathy (RD). To determine whether a correlation exists between decreasing ZMPSTE24 protease activity and increasing disease severity, we expressed mutant alleles of ZMPSTE24 in yeast and optimized in vivo yeast mating assays to directly compare the activity of alleles associated with each disease category. We also measured the activity of yeast crude membranes containing the ZMPSTE24 mutant proteins in vitro. We determined that, in general, the residual activity of ZMPSTE24 patient alleles correlates with disease severity. Complete loss-of-function alleles are associated with RD, whereas retention of partial, measureable activity results in MAD-B or severe progeria. Importantly, our assays can discriminate small differences in activity among the mutants, confirming that the methods presented here will be useful for characterizing any new ZMPSTE24 mutations that are discovered.


Subject(s)
Contracture/genetics , Craniofacial Abnormalities/genetics , Lipodystrophy/genetics , Membrane Proteins/genetics , Metalloendopeptidases/genetics , Mutation , Progeria/genetics , Proteolysis , Skin Abnormalities/genetics , Alleles , Amino Acid Sequence , Contracture/enzymology , Craniofacial Abnormalities/enzymology , Lipodystrophy/enzymology , Membrane Proteins/chemistry , Membrane Proteins/metabolism , Metalloendopeptidases/chemistry , Metalloendopeptidases/metabolism , Models, Molecular , Progeria/enzymology , Saccharomyces cerevisiae/enzymology , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae Proteins/chemistry , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism , Skin Abnormalities/enzymology
6.
PLoS One ; 7(2): e32120, 2012.
Article in English | MEDLINE | ID: mdl-22355414

ABSTRACT

BACKGROUND: The proteolytic maturation of the nuclear protein lamin A by the zinc metalloprotease ZMPSTE24 is critical for human health. The lamin A precursor, prelamin A, undergoes a multi-step maturation process that includes CAAX processing (farnesylation, proteolysis and carboxylmethylation of the C-terminal CAAX motif), followed by ZMPSTE24-mediated cleavage of the last 15 amino acids, including the modified C-terminus. Failure to cleave the prelamin A "tail", due to mutations in either prelamin A or ZMPSTE24, results in a permanently prenylated form of prelamin A that underlies the premature aging disease Hutchinson-Gilford Progeria Syndrome (HGPS) and related progeroid disorders. METHODOLOGY/PRINCIPAL FINDINGS: Here we have investigated the features of the prelamin A substrate that are required for efficient cleavage by ZMPSTE24. We find that the C-terminal 41 amino acids of prelamin A contain sufficient context to allow cleavage of the tail by ZMPSTE24. We have identified several mutations in amino acids immediately surrounding the cleavage site (between Y646 and L647) that interfere with efficient cleavage of the prelamin A tail; these mutations include R644C, L648A and N650A, in addition to the previously reported L647R. Our data suggests that 9 of the 15 residues within the cleaved tail that lie immediately upstream of the CAAX motif are not critical for ZMPSTE24-mediated cleavage, as they can be replaced by the 9 amino acid HA epitope. However, duplication of the same 9 amino acids (to increase the distance between the prenyl group and the cleavage site) impairs the ability of ZMPSTE24 to cleave prelamin A. CONCLUSIONS/SIGNIFICANCE: Our data reveals amino acid preferences flanking the ZMPSTE24 cleavage site of prelamin A and suggests that spacing from the farnesyl-cysteine to the cleavage site is important for optimal ZMPSTE24 cleavage. These studies begin to elucidate the substrate requirements of an enzyme activity critical to human health and longevity.


Subject(s)
Cell Nucleus/metabolism , Membrane Proteins/physiology , Metalloendopeptidases/physiology , Nuclear Proteins/metabolism , Protein Precursors/metabolism , Protein Prenylation , Proteolysis , Aging, Premature , Amino Acid Sequence , Animals , Blotting, Western , Cells, Cultured , Fibroblasts/cytology , Fibroblasts/metabolism , Humans , Kidney/cytology , Kidney/metabolism , Lamin Type A , Mice , Mice, Knockout , Molecular Sequence Data , Mutation/genetics , Plasmids/genetics , Sequence Homology, Amino Acid
7.
Nat Rev Mol Cell Biol ; 11(11): 759-63, 2010 11.
Article in English | MEDLINE | ID: mdl-20966969

ABSTRACT

Transport protein particle (TRAPP; also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor for the yeast GTPase Ypt1 and its mammalian homologue, RAB1, regulates multiple membrane trafficking pathways. TRAPP complexes exist in three forms, each of which activates Ypt1 or RAB1 through a common core of subunits and regulates complex localization through distinct subunits. Whereas TRAPPI and TRAPPII tether coated vesicles during endoplasmic reticulum to Golgi and intra-Golgi traffic, respectively, TRAPPIII has recently been shown to be required for autophagy. These advances illustrate how the TRAPP complexes link Ypt1 and RAB1 activation to distinct membrane-tethering events.


Subject(s)
Guanine Nucleotide Exchange Factors/metabolism , Vesicular Transport Proteins/metabolism , rab1 GTP-Binding Proteins/metabolism , Animals , Carrier Proteins/genetics , Carrier Proteins/metabolism , Endoplasmic Reticulum/metabolism , Golgi Apparatus/metabolism , Guanine Nucleotide Exchange Factors/genetics , Humans , Intercellular Signaling Peptides and Proteins , Models, Biological , Mutation , Vesicular Transport Proteins/genetics
8.
Mol Biol Cell ; 20(19): 4205-15, 2009 Oct.
Article in English | MEDLINE | ID: mdl-19656848

ABSTRACT

The GTPase Rab1 regulates endoplasmic reticulum-Golgi and early Golgi traffic. The guanine nucleotide exchange factor (GEF) or factors that activate Rab1 at these stages of the secretory pathway are currently unknown. Trs130p is a subunit of the yeast TRAPPII (transport protein particle II) complex, a multisubunit tethering complex that is a GEF for the Rab1 homologue Ypt1p. Here, we show that mammalian Trs130 (mTrs130) is a component of an analogous TRAPP complex in mammalian cells, and we describe for the first time the role that this complex plays in membrane traffic. mTRAPPII is enriched on COPI (Coat Protein I)-coated vesicles and buds, but not Golgi cisternae, and it specifically activates Rab1. In addition, we find that mTRAPPII binds to gamma1COP, a COPI coat adaptor subunit. The depletion of mTrs130 by short hairpin RNA leads to an increase of vesicles in the vicinity of the Golgi and the accumulation of cargo in an early Golgi compartment. We propose that mTRAPPII is a Rab1 GEF that tethers COPI-coated vesicles to early Golgi membranes.


Subject(s)
COP-Coated Vesicles/metabolism , Coat Protein Complex I/metabolism , Vesicular Transport Proteins/metabolism , rab1 GTP-Binding Proteins/metabolism , Animals , COS Cells , Chlorocebus aethiops , Coat Protein Complex I/genetics , Cytosol/metabolism , Cytosol/ultrastructure , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Guanine Nucleotide Exchange Factors/genetics , Guanine Nucleotide Exchange Factors/metabolism , Mice , Microscopy, Electron , Microscopy, Fluorescence , NIH 3T3 Cells , RNA Interference , Vesicular Transport Proteins/genetics , rab1 GTP-Binding Proteins/genetics
9.
Biol Chem ; 390(8): 761-73, 2009 Aug.
Article in English | MEDLINE | ID: mdl-19453269

ABSTRACT

ZMPSTE24 is an integral membrane zinc metalloprotease originally discovered in yeast as an enzyme (called Ste24p) required for maturation of the mating pheromone a-factor. Surprisingly, ZMPSTE24 has recently emerged as a key protease involved in human progeroid disorders. ZMPSTE24 has only one identified mammalian substrate, the precursor of the nuclear scaffold protein lamin A. ZMPSTE24 performs a critical endoproteolytic cleavage step that removes the hydrophobic farnesyl-modified tail of prelamin A. Failure to do so has drastic consequences for human health and longevity. Here, we discuss the discovery of the yeast and mammalian ZMPSTE24 orthologs and review the unexpected connection between ZMPSTE24 and premature aging.


Subject(s)
Aging, Premature/genetics , Membrane Proteins/physiology , Metalloendopeptidases/physiology , Progeria/genetics , Saccharomyces cerevisiae Proteins/physiology , Amino Acid Sequence , Animals , Enzyme Inhibitors/therapeutic use , Farnesyltranstransferase/antagonists & inhibitors , HIV Protease Inhibitors/pharmacology , Humans , Lamin Type A/genetics , Lamin Type A/metabolism , Lipoproteins/metabolism , Membrane Proteins/genetics , Metalloendopeptidases/genetics , Mice , Molecular Sequence Data , Nuclear Proteins/metabolism , Pheromones/metabolism , Progeria/drug therapy , Protein Precursors/metabolism , Saccharomyces cerevisiae Proteins/metabolism
10.
Mol Biol Cell ; 19(12): 5398-408, 2008 Dec.
Article in English | MEDLINE | ID: mdl-18923140

ABSTRACT

Proteins establish and maintain a distinct intracellular localization by means of targeting, retention, and retrieval signals, ensuring most proteins reside predominantly in one cellular location. The enzymes involved in the maturation of lamin A present a challenge to this paradigm. Lamin A is first synthesized as a 74-kDa precursor, prelamin A, with a C-terminal CaaX motif and undergoes a series of posttranslational modifications including CaaX processing (farnesylation, aaX cleavage and carboxylmethylation), followed by endoproteolytic cleavage by Zmpste24. Failure to cleave prelamin A results in progeria and related premature aging disorders. Evidence suggests prelamin A is imported directly into the nucleus where it is processed. Paradoxically, the processing enzymes have been shown to reside in the cytosol (farnesyltransferase), or are ER membrane proteins (Zmpste24, Rce1, and Icmt) with their active sites facing the cytosol. Here we have reexamined the cellular site of prelamin A processing, and show that the mammalian and yeast processing enzymes Zmpste24 and Icmt exhibit a dual localization to the inner nuclear membrane, as well as the ER membrane. Our findings reveal the nucleus to be a physiologically relevant location for CaaX processing, and provide insight into the biology of a protein at the center of devastating progeroid diseases.


Subject(s)
Amino Acid Motifs , Cell Nucleus/metabolism , Nuclear Proteins/metabolism , Protein Precursors/metabolism , Protein Processing, Post-Translational , Alkyl and Aryl Transferases/genetics , Alkyl and Aryl Transferases/metabolism , Animals , Cell Nucleus/ultrastructure , Endoplasmic Reticulum/metabolism , HeLa Cells , Humans , Lamin Type A , Membrane Proteins/genetics , Membrane Proteins/metabolism , Metalloendopeptidases/genetics , Metalloendopeptidases/metabolism , Mice , Mice, Knockout , NIH 3T3 Cells , Nuclear Envelope/metabolism , Nuclear Envelope/ultrastructure , Nuclear Proteins/genetics , Protein Methyltransferases/genetics , Protein Methyltransferases/metabolism , Protein Precursors/genetics , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism
12.
J Biol Chem ; 278(22): 19878-84, 2003 May 30.
Article in English | MEDLINE | ID: mdl-12657649

ABSTRACT

Here we report that Yip1p and Yif1p, two members of an integral membrane protein complex that bind to the Rab Ypt1p, are required for membrane fusion with the Golgi in vitro. To block fusion, anti-Yip1p or anti-Yif1p antibodies must be added before vesicles bud from the endoplasmic reticulum (ER). These antibodies do not block the packaging of Yip1p, Yif1p, or the soluble NSF attachment protein receptor (SNAREs) into vesicles. We propose that Yip1p and Yif1p perform a critical role in establishing the fusion competence of ER to Golgi vesicles at the time of budding. Consistent with this proposal, we observe that the Yip1p.Yif1p complex binds to the ER to Golgi SNAREs Bos1p and Sec22p, two components of the membrane fusion machinery.


Subject(s)
Endoplasmic Reticulum/metabolism , Fungal Proteins/metabolism , Saccharomyces cerevisiae Proteins/metabolism , Adaptor Proteins, Vesicular Transport , Animals , Antibodies/immunology , Fungal Proteins/immunology , Golgi Apparatus/metabolism , Membrane Fusion/immunology , Membrane Proteins , Rabbits , Saccharomyces cerevisiae Proteins/immunology , Vesicular Transport Proteins
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