[In vivo fluorimetry in physiological studies and clinical practice]. / Prizhiznennaia fliuorimetriia v fiziologii i klinike.

The paper describes two devices (an intraoperative luminescence probe and a medical fiberoptic spectrofluorimeter) to be used for fluorimetry of visceral tissues in man and animals and presents the results of their use in experiments and clinical practice. The application spheres of lifetime fluorimetry in physiological studies are dealt with.

[Optimized recording of results of the lymphocytotoxicity test using a luminescent attachment to Biolam-P-I microscope]. / Optimiziirovannyi uchet rezul'tatov limfotsitotoksicheskogo testa s pomoshch'iu liuminestsentnoi nasadki k mikroskopu "Biolam-P-I".

The fluorescent attachment appreciably simplifies the procedure of recording the results of the lymphocytotoxic test, is compact, economic, and easily inserted in the Biolam-P-I inverted microscope. Use of fluorescent stains helps selectively stain viable and intoxicated cells, thus simplifying their estimation. The said modification may be used in HLA typing and assessment of the principal lymphocyte subpopulations in the peripheral blood.

[A comparative analysis of the action of oxidative metabolic inhibitors on the luminescence parameters of normal and tumorous cells]. / Sravnitel'nyi analiz deistviia ingibitorov okislitel'nogo metabolizma na liuminestsentnye parametry normal'nykh i opukholevykh kletok.

Comparative investigation of different mitochondrial oxidative metabolism inhibitors action on NAD(P)H and flavoproteins fluorescence intensity of minimal transformed 3T3 NIH mouse fibroblasts and rat HTC hepatoma cells was made. Principle differences were shown between these cells in oxidized flavoproteins fluorescence intensity changes under the action of used inhibitors. It is suggested that the unusual HTC hepatoma cells flavin fluorescence intensity increase is connected with the oxidation of unidentified flavin-containing component functionally attached to mitochondrial respiratory chain.

[A photographic microfluorimetric method for determining the DNA content in individual human chromosomes]. / Fotograficheskii mikrofluorimetricheskii metod opredeleniia soderzhaniia DNK v individual'nykh khromosomakh cheloveka.

A method for determination of DNA contents in individual human chromosomes has been elaborated based of the two step analysis: 1) identification of chromosomes by Q-banding; 2) photographic microfluorimetry of chromosomes after the Feulgen staining (a fluorescent variant using a Schiff-type reagent of Auramine-SO2). The DNA content in 24 human chromosomes was calculated in absolute values (fg). The data obtained are compared with the evidence published elsewhere and provided by different cytophotometric methods.

[Experimental endofluorimeters for evaluating the intensity of luminescence of tissues in normal and pathological conditions during endoscopic studies and surgical interventions]. / Eksperimental'nye éndofluorimetry dlia otsenki intensivnosti liuminestsentsii tkanei v norme i pri patologii pri éndoskopicheskikh issledovaniiakh i operativnykh vmeshatel'stvakh.

Various analogs of devices recording the fluorescence intensity of tissues directly in the body chambers (fluorescent endoscopes/photometers, microfluorometers for investigation of biopsies and intraoperative probes) are described.

[Inhibitory analysis of the functions of the oxidative metabolic systems of tumor cells in tissue culture]. / Ingibitornyi analiz funktsional'nykh osobennostei sistem okislitel'nogo metabolizma opukholevykh kletok v kul'ture tkanei.

As has been previously reported an increased intensity of light-induced green fluorescence is observed for some tumor cells. The present paper deals with the cause of this phenomenon, employing for this hepatoma cells of line HTC acted upon with 2,4-DNP, amytal and malonate. It has been shown that the light-induced increase in green fluorescence in cells is due to the oxidation of NADH-dehydrogenase, a mitochondrial flavine-containing enzyme, occurring at the time of fluorescence induction. The increased intensity of green fluorescence of flavoproteins in tumor cells is associated with an infringement in oxidation of NAD-dependent substrates in these, and with the activation of the reverse electron transport in the oxidative chain. The exciding light activates NADH-dehydrogenase and accelerates the translocation of reduced equivalents from this enzyme, which results in its oxidation, and thus--in the observed effect of increased intensity of green fluorescence.

[Microfluorimeter for chromosome study]. / Mikrofluorimetr dlia issledovaniia khromosom.

A microfluorimeter is described for estimating amounts of chemical components in individual cells or in chromosomes, and for registering these components' distribution along the chromosomes. To solve the former problem, photoelectrical photometry of the object's fluorescence intensity is employed. To solve the latter problem, the photographic technique is used--making photos of metaphase plates in automatic or semiautomatic regime of exposure, with the following measuring of the intensity of the chromosome image on the negative performed on the same apparatus. The results of estimation of DNA content in individual chromosomes of Muntiacus muntjak are presented.

[Characteristics of "photo depletion" of the green fluorescence of tumor cells]. / Ob osobennostiakh "fotovygoraniia" zelenoi fluorestsentsii nekotorykh opukholevykh kletok.

The green (oxidized flavoproteins) fluorescence intensity was found to increase during investigation of NADH and oxidized flavoproteins fluorescence with the use of optimal excitation of different fluorescence bands. This effect was observed under excitation with blue light (436 nm). It is suggested that in some malignant cells, the structure of flavoproteins (probably of mitochondrial ones) may be altered in the way of increasing the quantum yield under the action of light irradiation.

[Spectral luminescence studies of cells stained with acrichine derivatives]. / Spektral'nye liuminestsentnye issledovaniia kletok, okrashennykh proizvodnymi akrikhina.

Excitation and fluorescence spectra are given of quinacrine derivative solutions, of buccal epithelium cell nuclei, of peripheral blood cells, and of isolated chromosomes treated with propyl-quinacrine mustard. It is confirmed that the differential cell treatment with quinacrine derivates may be observed in aqueous solutions only. Data obtained allow us to give some recommendations for employment of optimal filters and dichroic beam-splitters in the fluorescence microscopy of chromosomes treated with quinacrine derivatives.

[New measurement standard for cytofluorimetry]. / Novyi étalon dlia tsitofluorimetrii.

New phosphor crystals, on the basis of zeolit, activated by tin (Sn), appeared to be a very suitable permanent fluorescent test-object for setting up microscopes, intructing new users and for calibrating microfluorimeters. Their properties provide the control of conditions of various fluorescent experiments in both visible and ultraviolet regions. The crystal size may vary from 1 to 100 micron along the facet. Spectral, polarization and fading fluorescence characteristics of the new crystals are described.

[Recording of azimuthal characteristics of the polarized fluorescence of microstructures using a semi-wave plate]. / Registratsiia azimutal'nykh kharakteristik poliarizovannoi fluorestsentsii mikrostruktur s pomoshch'iu poluvolnovoi plastinki

We have suggested and grounded theoretically application of lambda/2 plate for the registration azimuth characteristics (AC) of polarized fluorescence of microstructures. Proper device of polarization microfluorimeter has been worked out. Application of this device in case of transmitted light illumination has allowed to record AC of a very small objects (up to 1 mkm) and add to a-curacity of measurements. In the case of incident light illumination one need to make achromatic lambda/2 plates.