1.
Proc Natl Acad Sci U S A
; 74(10): 4248-50, 1977 Oct.
Article
in English
| MEDLINE
| ID: mdl-200910
ABSTRACT
Horse heart cytochrome c can be split with cyanogen bromide into a heme peptide (residues 1-65) and a nonheme peptide (residues 66-104). In a process involving (i) complex formation between the two fragments and (ii) restoration of the severed peptide linkage, a fully active cytochrome c preparation can be re-formed. Use has been made of this process to couple the heme peptide to peptide 66-104 synthesized by the Merrifield solid-phase procedure. The semisynthetic product formed in this manner is indistinguishable from reconstituted cytochrome c prepared with nonsynthetic peptide 66-104.
Subject(s)
Cytochrome c Group/chemical synthesis , Amino Acids/analysis , Animals , Chemical Phenomena , Chemistry , Cytochrome c Group/analysis , Horses , Myocardium/enzymology , Peptides/analysis , Peptides/chemical synthesis , Radioimmunoassay
2.
Anal Chem
; 44(2): 343-50, 1972 Feb.
Article
in English
| MEDLINE
| ID: mdl-5059082