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Proc Natl Acad Sci U S A ; 74(10): 4248-50, 1977 Oct.
Article in English | MEDLINE | ID: mdl-200910

ABSTRACT

Horse heart cytochrome c can be split with cyanogen bromide into a heme peptide (residues 1-65) and a nonheme peptide (residues 66-104). In a process involving (i) complex formation between the two fragments and (ii) restoration of the severed peptide linkage, a fully active cytochrome c preparation can be re-formed. Use has been made of this process to couple the heme peptide to peptide 66-104 synthesized by the Merrifield solid-phase procedure. The semisynthetic product formed in this manner is indistinguishable from reconstituted cytochrome c prepared with nonsynthetic peptide 66-104.


Subject(s)
Cytochrome c Group/chemical synthesis , Amino Acids/analysis , Animals , Chemical Phenomena , Chemistry , Cytochrome c Group/analysis , Horses , Myocardium/enzymology , Peptides/analysis , Peptides/chemical synthesis , Radioimmunoassay
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